The Vehicle Routing Problem (VRP) is among the most complex optimization problems. Practical solutions require addressing real-world constraints such as time windows, vehicle capacities, delivery restrictions, driver working hours, and heterogeneous vehicle fleets. Solutions are often implemented in two stages: the first involves clustering customers, while the second focuses on incremental routing of these clusters to reduce complexity and improve solution control and explainability. However, the second stage heavily depends on the quality of the first, and clustering methods vary depending on client requirements. This paper explores various clustering methods and their impact on the final routing results, with a focus on real-world examples. The study includes diverse client scenarios, ranging from small-scale distribution systems with a limited number of customers to large-scale operations managing more than thousand of deliveries daily, covering both small and large orders. From fixed clustering and geographic partitioning to dynamic clustering algorithms and hybrid approaches, the advantages and limitations of each method are analyzed. The findings aim to provide actionable insights into selecting clustering methods that align with specific use cases, ensuring enhanced efficiency and adaptability in practical applications.

The paper examines the impact of budgetary accounting organization on the perception of corruption in the public sector, focusing on three key independent variables: the financial reporting framework, the accounting basis, and the level of independence of state auditing. The Corruption Perceptions Index (CPI), which measures the perceived level of corruption in the public sector, is used as an indicator of the dependent variable. The study includes data from 89 countries. For statistical analysis, categorical independent variables were encoded using the one-hot encoding method. Statistical tests were applied to assess the correlation between the independent variables and the CPI. The results show variations in correlation depending on the combination of financial reporting factors, the regulatory framework, and the quality of state auditing. The obtained results of multiple linear regression indicate that the model has a statistically significant impact on the CPI (p = 0.0217) and explains 21% of its variability. Keywords: public sector accounting, budgetary accounting organization, perception of corruption, public financial management reform.

This study aimed to compare anthropometric characteristics, body composition, and physical performance parameters between more and less experienced wrestlers to determine the influence of competitive experience on key performance indicators. A total of 47 male wrestlers were divided into two groups based on their competitive experience: more experienced (n=22, >6 years) and less experienced (n=25, <6 years). Anthropometric measurements included body mass, height, body mass index (BMI), and body fat percentage (BF%). Performance assessments consisted of the countermovement jump (CMJ), handgrip strength (HGS), and the Specific Wrestling Fitness Test (SWFT). Group differences were analyzed using t-tests for independent samples, and discriminant canonical analysis. More experienced wrestlers had significantly lower body fat percentage (p=0.04) and better performance in the SWFT repetitions test (p=0.001) compared to their less experienced counterparts. They also exhibited a lower heart rate response during the SWFT (p=0.01), indicating better recovery capacity. Also, competing experience was correlated to SWFT repetitions (R=0.51) and SWFT index (R=-0.45). Wrestling experience appears to be associated with wrestling-specific performance. However, the experience did not significantly influence absolute strength or explosive power. These findings suggest that training programs for less experienced wrestlers should emphasize wrestling-specific performance development to improve performance.

Abstract In the context of increasing challenges in the fight against financial crime, this paper analyzes the significance of forensic accounting in identifying suspicious transactions within the financial sector, with the aim of strengthening efforts to combat money laundering in Bosnia and Herzegovina (BiH). Money laundering is a complex form of financial crime that can be committed by individuals or large organizations. This form of financial crime is known from individuals to large organizations, and over time, different methodologies for carrying it out have developed. The detection and prevention of money laundering and the financing of terrorist activities in BiH are regulated by the Law on the Prevention of Money Laundering and Financing of Terrorist Activities (“Official Gazette of BiH,” No. 13/24), as well as the Rulebook on the Implementation of the Law on the Prevention of Money Laundering and Financing of Terrorist Activities (“Official Gazette of BiH,” No. 41/15 and 24/23). This paper aims to identify weaknesses in the financial and regulatory oversight systems in BiH. It also explores the level of understanding of forensic accounting techniques and money laundering mechanisms within organizations in the country, and assesses their impact on the overall effectiveness of anti-money laundering efforts.

The Constitution of Bosnia and Herzegovina is primarily notable for being an annex to the Dayton Peace Treaty that ended the 1992–1995 war. A significant aspect of this constitutional framework is its emphasis on the three main ethnic groups – Bosniaks, Croats, and Serbs – designated as constituent peoples, which play a central role in the state’s governance and institutions. The recent decision of the European Court of Human Rights (ECtHR) in the case of Savickis and Others v. Latvia has indirectly highlighted the (in)compatibility of certain constitutional solutions in Bosnia and Herzegovina with the principle of non-discrimination of the European Convention on Human Rights. In this article, the authors explain how the Constitutional Court of Bosnia and Herzegovina did not seek to implement the rulings issued by the ECtHR, but rather interpreted the constitutional identity of the country as being based precisely on the central role given to the constituent peoples. In so doing, the Constitutional Court, now also supported by the case Savickis and Others v. Latvia, created the prospect of abuse of constitutionalism in Bosnia and Herzegovina.

Background/Objectives: Colistin is a last-resort treatment for Pseudomonas aeruginosa multidrug-resistant infections, but resistance to it is emerging. While colistin resistance in P. aeruginosa is typically associated with chromosomal mutations inducing lipopolysaccharide (LPS) aminoarabinosylation, other mutations unrelated to LPS modifications have been proposed to influence the extent of colistin resistance. Here, we examined whether the genetic background and culture conditions affect the evolution of high-level colistin resistance in this bacterium. Methods: We performed in vitro evolution experiments in the presence or absence of increasing colistin concentrations with two phylogenetically distant reference strains in a standard laboratory medium and in two media mimicking P. aeruginosa growth during lung or systemic infections. Resistance-associated mutations were identified by comparative genomics, and the role of selected mutated genes was validated by allele replacement, deletion, or conditional mutagenesis. Results: Most colistin-resistant mutants carried mutations in genes belonging to four functional groups: two-component systems controlling LPS aminoarabinosylation (PmrAB, PhoPQ), LPS biosynthesis, the production of the polyamine norspermidine, and fatty acid metabolism. No mutation was exclusively and invariably associated with a specific strain or medium. We demonstrated that norspermidine is detrimental to the acquisition of colistin resistance upon PmrAB activation and that impaired fatty acid biosynthesis can promote colistin resistance, even if it increases susceptibility to other antibiotics. Conclusions: The evolution of colistin resistance in P. aeruginosa appeared to be only marginally affected by the genetic background and culture conditions. Notably, mutations in fatty acid biosynthetic genes represent a newly identified genetic determinant of P. aeruginosa colistin resistance, warranting further investigation in clinical isolates.

This manuscript reports the synthesis and characterization of 19 novel heterostilbene carbamates, designed as selective butyrylcholinesterase (BChE) inhibitors with potential applications in the treatment of neurodegenerative disorders, particularly Alzheimer’s disease. The compounds were synthesized from resveratrol analogs, and their structures were confirmed by NMR spectroscopy, high-resolution mass spectrometry (HRMS), and single-crystal X-ray diffraction for selected derivatives (compounds 1 and 4). In vitro assays demonstrated high selectivity toward BChE over acetylcholinesterase (AChE), with compound 16 exhibiting exceptional inhibitory activity (IC50 = 26.5 nM). Furthermore, compound 16 showed moderate anti-inflammatory effects by inhibiting LPS-stimulated TNF-α production in peripheral blood mononuclear cells. In silico ADME(T) profiling revealed favorable pharmacokinetic properties and low mutagenic potential for the majority of compounds. Molecular docking and molecular dynamics simulations confirmed stable binding interactions within the BChE active site. These results highlight heterostilbene carbamates as promising lead structures for developing novel therapeutic agents targeting neurodegenerative diseases.

To address the issue of groundwater vulnerability management and analysis, an algorithm was developed based on which the necessary research was conducted in the broader area surrounding the water source Novoselija used for water supply of the population in the city of Banja Luka (Republic of Srpska, Bosnia and Herzegovina). Within the GIS, as one of the spatial data techniques, the GOD method for vulnerability assessment was used. Data visualization within the GIS program package, combined with the aforementioned GOD method, enabled a clearer understanding of the spatial distribution of groundwater vulnerability in this area, which made it possible to delineate different zones: from negligible to extreme vulnerability. The high degree of the parameter has been determined in the zone of the actual water source and is associated with terrace and gravels sediments. In the alluvial sediment zone, located south of the water source, vulnerability has been assessed as extreme. In this area, there is certainly a potential risk of groundwater vulnerability to all pollutants, with a very rapid impact in most contamination scenarios. From the east and the west side, the aquifer in question is confined by flysch deposits of Cretaceous age, which are hydrogeologically characterized as conditionally “waterless” parts of the terrain, and within them, the vulnerability has been classified as negligible. The results of this research are also important for later delineation of the sanitary protection zone of the aquifer in question.

Neuroinvasive arboviruses such as tick-borne encephalitis virus (TBEV), West Nile virus (WNV), Usutu virus (USUV), and Toscana virus (TOSV) have (re-)emerged with increasing incidence and geographic range. We analyzed the epidemiology of arboviral infections in Croatia during the 2024 transmission season. A total of 154 patients with neuroinvasive diseases (NID), 1596 horses, 69 dead birds, and 7726 mosquitoes were tested. Viral RNA was detected using RT-qPCR. IgM/IgG-specific antibodies were detected using commercial ELISA or IFA, with confirmation of cross-reactive samples by virus neutralization test. RT-qPCR-positive samples were Sanger sequenced. Arboviral etiology was confirmed in 33/21.42% of patients with NID. WNV was most frequently detected (17/11.03%), followed by TBEV (10/6.49%), USUV (5/3.24%), and TOSV (1/0.64%). WNV infections were reported in regions previously known as endemic, while in one continental county, WNV was recorded for the first time. USUV infections re-emerged after a six-year absence. In addition to human cases, acute WNV infections were recorded in 11/395 (2.78%) of horses and two dead crows. WNV IgG seropositivity was detected in 276/1168 (23.63%) and TBEV IgG seropositivity in 68/428 (15.88%) horses. None of the tested mosquito pools were positive for WNV and USUV RNA. Phylogenetic analysis showed the circulation of WNV lineage 2 and Usutu Europe 2 lineage. Climate conditions in 2024 in Croatia were classified as extremely warm, which could, at least in part, impact the quite intense arboviral season. The spreading of flaviviruses in Croatia highlights the need for continuous surveillance in humans, animals, and vectors (“One Health”).

Beetroot (Beta vulgaris L.) is a plant grown for its roots, which are used to obtain sugar, feed animals, and for human use. Beetroot skin, a by-product of food processing, is a significant source of bioactive compounds, including dietary fiber and antioxidants. The primary objective of this work was to utilize beetroot skin powder to produce value-added nougat. Analytical methods, like antioxidant activity tests, proximate analysis, and sensory assessments, are used to determine the impact of beetroot skin powder on the final product. The beetroot skin powder extract had a remarkable content of phytochemicals and antioxidant activity. The inhibitory effect of the extract was tested on enzymes linked to metabolic syndrome, oxidative stress, and inflammation. The beetroot skin powder extract inhibited α-glucosidase, α-amylase, lipase, and lipoxygenase enzymes. The characterization of value-added nougat illustrates the multifunctionality of beetroot peel powder within its composition, serving as a significant source of natural compounds with antioxidant, coloring, and flavoring properties. This enhances sensory attributes, including color, aroma, and texture, augmenting product diversity and consumer appeal. This is evidenced by the increase in the total content of betalains (3.77 ± 0.09 mg/g DW.) and polyphenols (69.48 ± 2.88 mg GAE/100 g DW.), which lead to high antioxidant activity (73.89 ± 3.65 mM Trolox/100 g DW.) for the nougat sample with 6% added beetroot powder. Thus, beetroot skin powder replaced chemically synthesized additives with antioxidants and natural pigments, improving life quality and implicitly capitalizing on beetroot processing by-products, supporting circular economy principles at the global level.