Multiple sclerosis (MS) is a chronic inflammatory neurodegenerative disorder that typically affects young adults and is primarily characterized by demyelinating lesions in the central nervous system (CNS). According to the Revised McDonald Criteria, the clinical diagnosis of MS can be established based on a combination of clinical observations, the presence of focal lesions in at least two distinct CNS areas on magnetic resonance imaging (MRI) and the detection of specific oligoclonal bands in the cerebrospinal fluid. Conventional MRI remains a cornerstone of MS diagnosis and disease monitoring, providing high-resolution assessments of lesion burden and brain atrophy. In addition, advanced MRI methods are increasingly applied in research settings to probe myelin integrity, iron deposition, and biochemical changes, with the potential to complement established diagnostic workflows in the future. Despite remarkable advances in the management of MS over the past two decades, complex differential diagnoses and the lack of effective imaging tools for therapy monitoring remain major obstacles, thus channeling the development of innovative molecular imaging probes that can be harnessed in clinical practice. Indeed, positron emission tomography (PET) has a significant potential to advance the contemporary diagnosis and management of MS. Given the solid body of evidence implicating myelin dysfunction in the pathophysiology of MS, myelin-targeted imaging probes have been developed, and are currently under clinical evaluation for MS diagnosis and therapy monitoring. In parallel, ligands for the 18 kDa translocator protein (TSPO) and the cannabinoid receptor type 2 (CB2R) have been employed to capture neuroinflammatory processes by visualizing microglial activation, while other tracers allow the assessment of synaptic integrity across various disease stages of MS. Further, PET probes have been employed to delineate the role of activated microglia and facilitate the assessment of synaptic dysfunction across all disease stages of MS. This review discusses the challenges and opportunities of translational molecular imaging by highlighting key molecular concepts that are currently leveraged for diagnostic imaging, patient stratification, therapy monitoring and drug development in MS. Moreover, we shed light on potential future developments that hold promise to advance our understanding of MS pathophysiology, with the ultimate goal to provide the best possible patient care for every individual MS patient.

Background:Irritable Bowel Syndrome (IBS), a disorder of gut-brain interaction, is diagnosed using symptom-based Rome criteria. These criteria classify IBS patients into four subtypes in accordance to their stool patterns. However, whether this subtyping approach is based on true differences in the underlying biology of IBS patients, is unclear. Volatile organic compounds (VOCs) in the faecal headspace reflect both the gut microbial and host intestinal intraluminal processes and thereby may be used to study pathophysiological differences between IBS and its subtypes. Methods:We profiled faecal headspace VOCs in a cohort of 164 patients with IBS and 143 healthy controls using gas chromatography-mass spectrometry (GC-MS). Random forest models were employed to impute missing values and identify discriminatory VOCs to differentiate IBS patients from healthy controls. We corrected for faecal water content using Partial Least Squares Regression. Multivariate associations between the obtained volatile profiles and Rome III IBS subtypes were evaluated using regularized MANOVA. Results:A total of 39 VOCs, including short-chain fatty acid esters, neurotransmitter-related metabolites, alcohols, and sulphides, were selected as significantly altered in patients with IBS. Our classification model achieved an area under the curve (AUC) of 0.82 on both training and independent test sets, demonstrating robust separation between IBS patients and healthy individuals. However, VOC profiles did not associate to Rome III -based IBS subtypes. Conclusion:This study highlights the potential of faecal VOC profiling as a non-invasive tool for studying and characterising IBS, yet they also reveal a disconnect between metabolic signatures and current stool-based subtypes. While the Rome criteria remain the clinical standard for diagnosis and subtyping of IBS, they offer limited insight into underlying disease mechanisms. Future research should focus on integrating VOC analysis with other omics approaches to refine IBS sub-classification into biologically relevant clusters, which may aid to improve personalised therapeutic strategies.

Cultural and event tourism plays a key role in the global tourism industry, contributing significantly to national, regional, and cross-border development strategies. It attracts visitors interested in cultural heritage, artistic events, and local traditions, supporting the preservation and promotion of local identity. Assessing cultural tourist flows become essential to understand their economic and social impact, and this article proposes an integrated methodology, combining quantitative and qualitative approaches, including data collection on cultural events, participant estimation, economic analysis, satisfaction assessment, data visualization, reporting, and continuous monitoring. The proposed model ensures a comprehensive evaluation of cultural tourist flows and supports the sustainable development of cultural tourism. Its relevance is both theoretical and applied: it offers a conceptual and analytical framework for studying this complex phenomenon, but also, it provides useful insights for decision-makers and stakeholders involved in tourism development, helping to design effective policies and strategies for promoting and managing cultural tourism. The novelty of the research lies in the proposal of a detailed and integrated methodology tailored to the specific challenges of evaluating cultural tourism, aiming to improve data accuracy, strategic planning, and long-term sustainability.

Many cyberattacks succeed because they exploit flaws at the human level. To address this problem, organizations rely on security awareness programs, which aim to make employees more resilient against social engineering. While some works have suggested that such programs should account for contextual relevance, the common praxis in research is to adopt a"general"viewpoint. For instance, instead of focusing on department-specific issues, prior user studies sought to provide organization-wide conclusions. Such a protocol may lead to overlooking vulnerabilities that affect only specific subsets of an organization. In this paper, we tackle such an oversight. First, through a systematic literature review, we provide evidence that prior literature poorly accounted for department-specific needs. Then, we carry out a multi-company and mixed-methods study focusing on two pivotal departments: human resources (HR) and accounting. We explore three dimensions: threats faced by these departments; topics covered in the security-awareness campaigns delivered to these departments; and delivery methods that maximize the effectiveness of such campaigns. We begin by interviewing 16 employees of a multinational enterprise, and then use these results as a scaffold to design a structured survey through which we collect the responses of over 90 HR/accounting members of 9 organizations. We find that HR is targeted through job applications containing malware and executive impersonation, while accounting is exposed to invoice fraud, credential theft, and ransomware. Current training is often viewed as too generic, with employees preferring shorter, scenario-based formats like videos and simulations. These preferences contradict the common industry practice of annual sessions. Based on these insights, we propose recommendations for designing awareness programs tailored to departmental needs and workflows.

Background: Peritoneal lymphangiomatosis is a rare benign lymphatic disorder with diagnostic and therapeutic challenges. It may mimic peritoneal carcinomatosis or mesothelioma, often requiring histological confirmation. Management options are limited, and long-term data on targeted therapies, including sirolimus, remain scarce. Case Presentation: We present the case of a 45-year-old woman with a history of surgically corrected atrial septal defect, who developed progressive abdominal distension and dysuria in May 2014. Abdominal ultrasound (US) and contrast-enhanced abdominal computed tomography (CT) revealed diffuse ascites, peritoneal thickening, and cystic changes. Diagnostic uncertainty led to an exploratory laparotomy in May 2015, where histopathology confirmed peritoneal lymphangiomatosis. After failed conservative management including dietary modification, diuretic therapy, and repeated paracenteses, she was started on sirolimus 2 mg/day in August 2016. Her serum trough levels were maintained between 5–15 ng/mL. She achieved a Karnofsky Performance Score of 100% (indicating full functional capacity) within two months and has remained in complete clinical and radiologic remission for over 8 years. Diabetes mellitus type 2 was diagnosed during follow-up and is well controlled with insulin therapy. Conclusion: This case underscores the difficulty of diagnosing peritoneal lymphangiomatosis and highlights the transformative potential of sirolimus therapy. To our knowledge, this is one of the longest documented remissions of peritoneal lymphangiomatosis under mechanistic target of rapamycin (mTOR) inhibition. Keywords: peritoneal lymphangiomatosis, sirolimus, mTOR inhibitor, rare disease, ascites, lymphatic malformation, case report, remission, Karnofsky score, targeted therapy.

The indoor environment of buildings is of fundamental importance for the health of people and other living organisms residing in them. From this perspective, key factors include indoor temperature, relative humidity and the concentration of CO2 or other pollutants. These healthy indoor conditions are typically maintained through functional heating and ventilation systems. However, in the case of indoor humidity, increasing moisture levels when they are low can be relatively challenging. There are more energy-efficient solutions that can be combined with ventilation systems. These include, for example, placing plants and green walls in the interior, which have a significant impact not only on microclimatic and acoustic conditions of the interior, but also on the overall psychological well-being of occupants. Green elements contribute to the effective regulation of CO2 and certain other harmful substances within the indoor environment. Another possible solution involves the use of sorption-active materials in the form of cladding panels—elements capable of functioning as indoor regulators, i.e., absorbing moisture and releasing it back into the indoor environment when necessary. This study investigates the moisture behavior of natural composites based on montmorillonite clay and straw fibers, as well as their possible integration with green elements to create healthy indoor conditions for their inhabitants. The developed clay composite can be classified as water and steam absorption class WSIII according to DIN 18948—the moisture buffering capacity value was 152.73 g/m2 after 12 h. Based on the research results, it can be stated that these composites could serve as interior cladding elements in synergy with green elements (Chlorophytum comosum, Epipremnum aureum), ideally regulating the indoor microclimatic conditions, especially as an effective solution for short-term humidity changes. The maximum difference in relative humidity between the reference testing chamber (without green elements and clay plates) and the chamber containing plant Chlorophytum comosum and three clay composite plates was 23.04%.

Shared administration – EU migration agencies – The European Border and Coast Guard Agency Frontex – European Integrated Border Management and responsibility gaps – Unclear division of obligations in Frontex’s mandate – EU composite legal order and limits of EU judicial remedies – National courts and the right to effective judicial protection under Article 19(1) TEU and Article 47 Charter – Domestic judges as European judges – Advantages and potential of domestic remedies in multi-actor situations – Importance of preliminary references in shared administration– Domestic adjudication of human rights violations in European Integrated Border Management – Adjudication on member state responsibility – Adjudication on individual responsibility – Strengthening domestic judicial systems.