The Tracking Cancer Evolution Through Therapy (TRACERx) program represents the most comprehensive effort to characterize tumor evolution in real time. Through longitudinal, multiregion, and multiomic profiling of tumors—and particularly of non-small-cell lung cancer and clear cell renal cell carcinoma—TRACERx has illuminated the dynamic interplay between genetic, nongenetic, and (micro)environmental factors that drive cancer progression, immune evasion, and therapeutic resistance. A central insight from TRACERx has been that not all tumor evolution is genomic: Transcriptomic diversity, epigenetic alterations, RNA editing, and changes in cell–cell interactions also drive adaptation. Methodological innovations—including tumor-informed and ultrasensitive circulating tumor DNA assays, representative sequencing, and integrative immune–genomic analyses—have yielded biomarkers resistant to sampling bias and/or predictive of recurrence, metastasis, and treatment response. By demonstrating that intratumor heterogeneity is a key determinant of clinical outcome and revealing its molecular, transcriptional, and ecosystem-level drivers, TRACERx has established a framework for linking evolutionary dynamics to patient care. As both a scientific framework and a clinical paradigm, TRACERx demonstrates how adaptive, iterative research can refine evolutionary models, improve patient risk stratification, and inspire next-generation cancer evolution studies across malignancies.

Purpose: To emphasize the diagnostic role of magnetic resonance imaging (MRI) in identifying Fitz-Hugh–Curtis syndrome (FHCS) in an adolescent patient with a subtleclinical presentation. Methods: A 17-year-old female presented with acute onset right upper quadrant and right shoulder pain. The initial diagnostic workup, including ultrasound (US), computedtomography (CT), and laboratory testing was inconclusive. Due to persistent symptoms and elevated inflammatory markers, MRI of the abdomen and pelvis was subsequentlyperformed. Results: MRI demonstrated a bilateral pyosalpinx with restricted diffusion, free fluid in the pelvis and subhepatic region, and linear adhesions between the hepatic capsule and peritoneum, which was consistentwith pelvic inflammatory disease (PID) complicated by FHCS. Microbiologic analysis confirmed a Chlamydia trachomatis infection. The patient received targeted antibiotic therapy with clinical improvement. Conclusion: FHCS represents a rare but importantcomplication of PID that may present with non-specificor extra-pelvic symptoms. MRI is the modality of choicefor confirming PID-related complications and perihepatic inflammation, offering high sensitivity and the advantage of radiation-free imaging, which is especially relevant in the adolescent population.

Lemon essential oil is widely used in both commercial and domestic products. It consists of a mixture of volatile, liposoluble compounds responsible for its characteristic fragrance and bioactive properties, including antimicrobial, anti-inflammatory and antioxidant activities. The importance of lemon essential oil is even more significant when considering the increasing interest in natural products that promote human health. In this study, domestically grown lemons were used. The essential oil was extracted from the peel using the hydrodistillation method, an environmentally friendly process. The composition of the resulting essential oil was analysed using gas chromatography coupled with mass spectrometry (GC-MS). The analysis revealed that d-limonene, β-pinene, and γ-terpinene are the predominant components found in lemon essential oil. The antimicrobial activity of the essential oil was tested and found to be effective against Staphylococcus aureus. Molecular docking analysis revealed that all three principal compounds were able to bind to the DNA gyrase enzyme, with d-limonene exhibiting the lowest binding energy. The essential oil also exhibited significant DPPH radical scavenging activity, highlighting its antioxidant potential. The results indicate that both concentration and incubation time affect the antioxidant capacity of the essential oil.

Previous studies show that interventions to improve attitudes toward stuttering yield inconsistent results on the Public Opinion Survey of Human Attributes–Stuttering (POSHA–S). Comparisons of pre- and post-intervention samples indicate that success depends on the percentage of respondents who improved rather than the magnitude of change. A “crossover” effect emerged: respondents with the most positive pre-test attitudes showed lower post-test ratings, whereas those with the most negative pre-test attitudes showed the greatest improvement; respondents with intermediate attitudes showed little change. Similar patterns appeared in non-intervention samples, where one-third fell into positive, minimal, or negative change groups. The study analyzed 943 respondents from 29 intervention samples classified as unsuccessful (U), marginally successful (MS), successful (S), or very successful (VS), plus 345 respondents from 12 non-intervention samples. Using non-intervention data as a baseline, we calculated percentages shifting among the three change groups. In the VS category, interventions moved people from the negative and minimal change groups into the positive change group. In the S category, gains in the positive change group came from the negative change group. In the MS category, all intervention-related changes began in the negative change group, yielding modest growth in the positive and minimal change group. The U category showed shifts into both the positive and negative change group, mainly reducing the minimal change group. These findings suggest that interventions to improve attitudes toward stuttering should apply strategies tailored to individuals in positive, minimal, and negative change groups.

Ovaj rad pruža (sveobuhvatnu) analizu razvoja rimskog kaznenog (krivičnog) postupka od kraljevskog doba do kraja Republike. Autor istražuje kako je rano rimsko društvo prešlo sa privatne osvete na državno provođenje pravde, naglašavajući nerazdvojivost prava, religije i politike. Kroz detaljno razmatranje institucija kao što su quaestores parricidii, quaestiones perpetuae, te reformi koje su sproveli Sula, Ciceron i Pompej, tekst prikazuje sve veću formalizaciju, specijalizaciju i politizaciju krivičnog prava. Posebna pažnja posvećena je procesnim principima, uključujući pretpostavku nevinosti, akuzatorni model i pravila dokazivanja. Zaključno, rimsko krivično pravo – iako često zapostavljeno u korist privatnog prava – imalo je značajan uticaj na temeljne koncepte savremenih evropskih  krivičnopravnih sistema.

Background: The symptoms of COPD differ based on the stage of the disease, becoming more noticeable in its severe stages and during exacerbations. They are associated with temporary incapacity for work and cause a decrease in working capacity. Also, occupations with special working conditions and exposure to pollutants at work cause increased bronchial secretion and cough. This research aims to identify the most common symptoms in COPD patients and evaluate their work capacity based on symptom prevalence. Methods: 200 subjects were included in the prospective study. Participants were divided into two groups: a study group and a control group. Within each group, subgroups were created based on smoking habits, a key risk factor for COPD development. Results: The dominant symptom in both groups was cough, with 34.67%. In subjects with preserved working capacity, the most common symptoms are chest pressure with 13.33% and shortness of breath with 9.33%, while in the group with reduced working capacity, fatigue (30%) and dyspnea with 26%. In the control group, most subjects had obstructive bronchitis, asthma and chronic simplex bronchitis. Conclusion: According to the GOLD classification, modified working capacity decreased by 51.33%, while retained working capacity declined by 48.67% in relation to disease severity. Cough was the dominant symptom in both groups, while other symptoms varied independently of the stage of the disease.

Dear editor,We would like to express our sincere gratitude for the attention given to our published scientific paper, as well as for the positive comments, valuable suggestions, and thoughtful questions in the Comment on the article by Bajpai. We consider his contribution extremely important for improving the quality of future research in this field.

Define the most common bacterial isolates of urinary tract infections and the difference in the antibiotic resistance before and during the coronavirus pandemic.Methods: The study included 213 patients with a diagnosis of urinary tract infection treated at the Clinic of infectious diseases in Mostar in a two-year period, 2019 and 2021. We analyzed the incidence of urinary infections, uropathogens and antibiotic resistance.Main findings: We had total of 213 patients, more female patients, the average age was 66 and over, with the most clinical diagnosis of acute cystitis. The most common bacterial isolates were E. coli, K. pneumoniae and Entercoccus spp. We showed in 2019 decrease the incidence of E. coli and the increase in incidence of K. pneumoniae in 2021. Antibiogram results showed an increase in the antibiotic resistance in 2021 for all bacterial isolates except for K. pneumoniae.Principal conclusion: The frequency of urinary tract infections was highest in older women with the most common diagnosis of acute cystitis. The most common bacterial pathogen of urinary tract infections are E. K. pneumoniae and Enterococcus spp. as the main cause of urinary tract infections and general an increase in the antibiotic resistance of uropathogens. Key words: Antibiotic resistance, E. coli, urinary tract infections, K. pneumoniae

Introduction: Breast cancer represents a global public health challenge, often diagnosed at advanced stages due to a lack of awareness and insufficient practice of breast self-examination (BSE). This study aims to assess and compare the knowledge, attitudes, and practices of female students regarding BSE in the context of breast cancer prevention and early detection. Methods: The cross-sectional study was conducted from March to April 2023 and included 151 female students from the Faculty of Health Studies and the Faculty of Economics and Business University of Sarajevo. The research instrument was a modified questionnaire based on an existing questionnaire from previous research and adapted to the objectives of this study. Results: The results showed that almost all participants were familiar with BSE, with no significant differences between faculties. Students from the Faculty of Health Studies were more likely to report knowing the right time for BSE (45.1%), while students from the Faculty of Economics and Business were more uncertain and relied more on information from the internet. The main reason for not performing the BSE was a lack of knowledge about the correct technique, which was most frequently stated by economics students. Nevertheless, the majority of participants (91.4%) recognized BSE as an important method for early detection of breast tumors, highlighting the need for further education to strengthen practical skills. Conclusion: The study found that although most students are aware of the importance of BSE, regular practice is low, mainly due to uncertainty about the correct technique. The results of this study show that there is a need to integrate targeted educational programs into university curricula and health promotion campaigns to reinforce preventive behaviors.

Workplace behaviors and employee outcomes, such as team functioning, job satisfaction, and intentions to leave, are crucial for healthcare quality and safety. It highlights the substantial productivity, societal, and economic costs of worker well-being. Against this backdrop, this study examines how two dimensions of organizational culture: ethical climate and perceived managerial competence, together with team support, relate to job satisfaction and turnover intention among healthcare professionals. A quantitative, cross-sectional survey was conducted with 430 physicians, nurses, and other clinical staff in public and private institutions across the Federation of Bosnia and Herzegovina. Using established scales and structural equation modeling (SEM) in AMOS, we first verified satisfactory reliability and construct validity via exploratory and confirmatory factor analyses. The structural model showed that ethical organizational culture and managerial competence are positively related to team support and, directly or indirectly, to higher job satisfaction and lower turnover intention. Team support was positively related to job satisfaction and negatively related to turnover intention and significantly mediated the effects of both ethical climate and managerial competence on these outcomes. In addition, job satisfaction was strongly and negatively correlated with turnover intention, underscoring its central role in retention.

The aim of this study was to investigate the effectiveness, tolerability and safety of using lisinopril tablets in the treatment of essential hypertension for a period of 12 weeks.Methods:A multicenter, observational, non-interventional, postmarketing clinical trial was conducted on adult patients older than 30 years with a diagnosis of hypertension and blood pressure values ≥ 140/90 mmHg. Patients taking lisinopril were monitored for 12 weeks, during one initial and three control examinations.Main findings: The study included 334 patients:group A-aged 30 to 59 (n=167) and group B -aged 60 and older (n=167). In the group of younger respondents, the systolic/diastolic pressure before therapy was on average 150/95 mmHg, and with the use of lisinopril therapy, decreased to an average of 130/80 mmHg after 4 weeks. For the older respondents, the systolic/diastolic pressure before therapy was on average 155/95 mmHg, while afterstarting the lisinopril therapy, it decreased to an average of 135/80 mmHg. The differences in systolic/diastolic pressure were assessed as very good, namely 70.9% in the older and 87.4% in the younger group. More patients from the elderly group (34 patients) had adverse events compared to the younger group (24 patients). Adherence was generally good. Comparison of the two groups showed better results in the younger group compared to older group, 82.6% vs 69.3%.Principal conclusion:Lisinopril showed goodefficacy and safety in reducing essential hypertension in patients. Tolerability and high adherence to lisinopril therapy was observed. Key words: Lisinopril, hypertension, systolic blood pressure, diastolic blood pressure

Heart-rhythm disorders and related congenital anomalies in children often remain silent until physical activitybrings on symptoms. We report an 11-year-old girl who experienced her first signs of heart disease only after exertion. Although late detection of such anomalies can sometimes be fatal, careful clinical examination may reveal these conditions early.

Background: A doubt that occupational medicine specialists face is whether exposure to solvents is an independent risk factor for non-alcoholic fatty liver disease. Aim: The present study investigates the connection between the length of work experience (exposure) at workplaces that are exposed to organic volatile substances with eventual changes in the morphological structure of the liver parenchyma or laboratory parameters of liver function, and to investigate whether organic volatile substances, as an independent factor can lead to liver steatosis or changes in laboratory parameters of liver function.  Methods: A prospective study was conducted from January to April 2023. and included 62 coke industry workers who were divided into two groups of 31 workers each (experimental with workers exposed to organic volatile substances at the workplace and control with workers who were not exposed). Laboratory parameters of liver function were monitored, as well as ultrasound findings of liver parenchyma in coke industry workers undergoing examination. Exclusion criteria were known history of liver disease, blood transfusion, alcohol consumption and diabetes mellitus. Results: Changes in laboratory parameters of liver function and ultrasound findings of liver steatosis were found with high statistical significance in the workers of the experimental group, for which no statistically significant association with elevated values of lipid status was found. With diabetes as an exclusion factor and the insignificant prevalence of hypertension in the examined workers, it can be concluded that metabolic syndrome is not related to our results. During the correlation analysis, it can be concluded that organic volatile substances are an independent cause and trigger for fatty infiltration of the liver parenchyma and changes in the laboratory findings of liver function parameters. Conclusions: Exposure to organic volatile substances at the workplace as an independent factor causes liver steatosis with elevated values of laboratory parameters of liver function and it can be said that it is an independent cause of non-alcoholic fatty liver disease.