Rhyacophila Pictet 1834 is globally distributed and highly diverse genus of caddisflies (Trichoptera), characterized by numerous regionally endemic species. In the Balkan Peninsula, the highest number of Rhyacophila species (23) was recorded for Bosnia and Herzegovina. Rhyacophila bosnica Schmid, 1970 is found only in the Balkan Dinaric region, with a locus typicus in Vučja Luka, Bosnia and Herzegovina. Like with many species of Trichoptera, the morphology of its larva is still unknown. Therefore, DNA barcoding approach was used to link two developmental stages. In this paper, we report on the first DNA barcode record for this species.

Inducing cell death in tumor cells has been recognized as a promising strategy in curing tumors. Parallely, natural products, especially those with long-known usage in folk medicine, are gaining demanding and extensive clinical interests. Aiming to contribute to overall knowledge of curcumin and luteolin antitumour potentials, we analyzed their effects on cell death induction in NFS-60 cell line, using Trypan blue exclusion assay and TransDetect® Anenexin V-EGFP/PI assay. Results show that both tested agents induce cell death, especially in higher applied concentrations, but further investigations are needed to elucidate the mechanisms behind it.

Apart from its physiological role in the cellular oxidation of ethanol interesting feature of the ADH1B gene locus is its characteristic geographical distribution in which certain variants of ADH1B peak in different parts of the world.  Therefore, ADH1B rs2066701 polymorphism is exploited as a genetic marker in tracing of the evolutionary processes and human migrations in the past. Taking into consideration the complexity of population genetic structure and several migrations in the history of the Balkan populations, including Bosnian and Herzegovinian, this study aimed to estimate the frequency of ADH1B rs2066701 polymorphism in the population of Bosnia and Herzegovina. The total of 101 randomly sampled individuals was genotyped for rs2066701 polymorphism in ADH1B gene using PCR-RFLP method. The obtained frequencies were used to calculate heterozygosity, fixation indices and Hardy-Weinberg equilibrium. Observed population-structure parameters were compared with other population values available in ALFRED database. Dimensional relations between the investigated populations were visualised with the NM-MDS (non metric multidimensional scaling) analysis using PAST. The minor allele frequency for rs2066701 was 0,257. Inter-population analysis including other European and non-European populations from the ALFRED database proved the above-mentioned European genetic background of the B&H population.

MODY (maturity-onset diabetes of the young) is an autosomal dominant form of diabetes that is usually manifested before the 25-year of life. This type of diabetes is caused by defects in the primary insulin secretion. There are several types of MODY, which are monogenic diseases, where mutations in a single gene are responsible for a particular type of MODY. Currently, there are eleven types of MODY, from which the most common types are MODY 2 and MODY 3 (with mutations on GCK and HNF1A genes, respectively). We identified very rare MODY 7 type of diabetes in three family members by MLPA analysis.

Following the “horse meat scandal” in 2013, European Union countries have conducted official control of EU market and unraveled food fraud which implicated a number of processed food products and food businesses. Five years after the breakout of the scandal, no official information on market surveillance in Bosnia and Herzegovina is available. Therefore, 73 randomly selected meat products from retail were collected and analyzed for the presence of horse DNA. Horse DNA was detected in 21 products (28.77%). Particularly disturbing for B&H consumers is high proportion of sujuk samples positive for horse DNA (46.15%) with lower incidence among the products of small manufactures. Also disturbing is the finding that 71.43% of the products that contain horse DNA were produced in B&H. According to our data there is a requirement for stricter surveillance of both import and internal market.

Bu calismanin amaci heavy hybrid hindilerin ince ve kalin bagirsaklarindaki duz kaslarda bulunan adrenerjik reseptorlerin olasi varligini in vitro kosullar altinda farkli konsantrasyonlarda non-selektif adrenerjik reseptor agonisti, noradrenalin, kullanarak belirlemektir. Calisma 24 besi hindisinde gerceklestirildi. Organ izolasyon cihazinda duodenum, jejunum, ileum, cekum ve kolonun muskuler longitudinal ve sirkuler tabakalarinda testler farkli konsantrasyonlarda noradrenalin hydrogentartarate solusyonu kullanilarak test edildi. Calisma besi hindilerinin ince ve kalin bagirsaklarindaki duz kaslarda bulunan adrenerjik reseptorlerin olasi varligini ortaya koymdu. Noradrenalin’in longitudinal tabaka uzerindeki etkisi, besi hindilerinin ince ve kalin bagirsaklarinda ki tunica muscularis sirkuler tabaka uzerinde ki etkisinden daha gucluydu (P<0.001). Farkli konsantrasyonlarda noradrenalin kullanimina iliskin olarak elde edilen sonuclarda da istatistiksel olarak anlamli farkliliklar vardi. Noradrenalinin ince ve kalin bagirsaklarin farkli bolgelerindeki etkilerinde de onemli farkliliklar bulundu. Selektif agonistler ve α ve β reseptor antagonistleri kullanilarak, besi hindilerinin ince ve kalin bagirsak duz kaslarinda daha fazla arastirma yapilmasi gerekmektedir.

As in many developing countries, Libya is still managing the solid waste improperly. This fact has led to increase the amount of solid wastes accumulated in the country. With low fuel costs, the companies make low consideration to the transportation cost. The research in municipal solid waste in Libya is rare, and focused on waste classification area. The objective of this paper is to evaluate the municipal solid waste management system in Misurata city, Libya, and to suggest a model which minimizes the total cost of waste management by adding a collection stations. The paper evalutes two models: in the first model only collection vehicles are used, and the waste transfered directly to the dumping site. In the second model collection sites are tested, and the best one is selected according to the total cost. ADD algorithm used in the second model. The second model showed that there is a reduction in the total distance travelled by the trucks up to 45%.

Biodiesel fuel is the realistic fuel for the future due to its environmental, economic and energetic benefits. However, the degradation and instability during biodiesel storage and application present the major disadvantages and hence a modern scientific challenge. The aim of this study was to choose optimal parameters for biodiesel synthesis from fresh and waste sunflower cooking oil and to investigate the possibilities of increasing its resistance towards oxidative degradation. Various physico-chemical characteristics essential for the quality estimation of the resulting biodiesel products were compared before testing the effectiveness of natural and synthetic antioxidants. Butylated hydroxytoluene (BHT), carvacrol and α-tocopherol were added to the biodiesel in different concentrations in order to determine their efficiency during the Schaal oven test. Results proved that both, fresh and waste oil can be valuable sources for the synthesis of biodiesel that meets European and American quality standards. Among the antioxidants, BHT was the most efficient one in both types of biodiesel and its usage would be recommended at the concentration of 1000 ppm. The findings present a cost-effective and environmentally friendly source for biodiesel production with improved properties - considerably enhanced resistance to oxidative degradation, where synthetic antioxidants are given the priority. DOI: http://dx.doi.org/10.17807/orbital.v10i7.1174

Cadmium (Cd) is abundant heavy metal with highly toxic effect on plants. The aim of presented study was to investigate the effect of seed priming using salicylic acid on cadmium tolerance in lettuce. The tolerance level was evaluated using germination speed and percentage, fresh and dry seedling weight, water and photosynthetic pigments content. Control seeds were primed using distilled water. In control plants cadmium induced reduction of germination rate, fresh and dry weight, water and chlorophyll content. Seed priming using salicylic acid, on the other hand, induced increased resistance of lettuce to cadmium toxicity. Increase in germination rate, fresh mass and water content were recorded for plants subjected to 5 mM Cd, grown from seeds primed using salicylic acid. Results obtained by this study suggested that salicylic acid can be used as priming agent in order to alleviate Cd toxicity on lettuce.

Antifungal, antimicrobial, insecticidal and antioxidant activities of Origanum vulgare L. provide the basis for suggesting that oregano plant extracts may be useful for prevention and treatment of many infection. The main goal of this study was to determine antimicrobial and antioxidant activities of methanolic and aqueous extracts from the leaves and flowers of Origanum vulgare. Antimicrobial testing of plant extracts was done using well diffusion method. Activity of extracts were tested against Gram positive bacteria: Staphylococcus aureus ATCC 25923, methicillin-resistant Staphylococcus aureus (MRSA) ATCC 33591, Bacillus subtilis ATCC 6633, Enterococcus faecalis ATCC 29212 and five Gram-negative bacteria: Salmonella abony ATCC 6017, Salmonella enterica serovar Enteritidis ATCC 31194, Pseudomonas aeruginosa ATCC 9027, Escherichia coli ATCC 25922, extended-spectrum β-lactamase (ESBL) producing Escherichia coli ATCC 35218 and fungi Candida albicans ATCC 1023. Antibiotics ampicillin, streptomycin and antimycotic nystatin were used as positive controle. The antioxidant activity was determined by using the DPPH (1,1-diphenyl-2-picrylhydrazyl) method. The highest values for inhibition zone for methanolic and aqueous extracts were recorded for Gram positive Bacillus subtilis, Staphylococcus aureus and MRSA. Methanolic extracts exhibited antibacterial activity against tested Gram negative bacteria in variable degree while the growth of these bacteria was not inhibited by aqueous extracts. Tested fungi Candida albicans was not susceptible to investigated oregano extracts. All the extracts showed moderate to potent antioxidant activity, among which the methanolic flower extract demonstrated the strongest antioxidant activity with the IC50 value of 0.205 mg/mL. Therefore it can be concluded that flower and leaf oregano extracts have great antibacterial and antioxidant potential.

Background: Children with autism in their characteristics show a series of unusual reactions to stimuli in all areas of the sensory system. Aim: The aim of this paper was to compare the tactile and auditory processes, i.e. to determine the deficits of these processes by children with autism spectrum disorder (Diagnostic and Statistical Manual of Mental Disorders, 5th ed.) in relation to children with intellectual disabilities and children of the typical population. Methods: The sample consisted of a total of 105 children. During the survey, the method of proportional stratified sample was used and the data collection was carried out in 2017 on the entire territory of Bosnia and Herzegovina. Short Sensory Profile was applied (Dunn, 1999) and through 13 items, Tactile Perception and Hearing Perception were examined. Results: It was found that 71.4% of children with autism had significant difficulties in the area of tactile perception and 65.7% in the area of hearing perception. Tactile and hearing sensitivity is also common by children with intellectual disabilities, which undermines the inclusion of the difficulty of sensory processing as a key diagnostic criterion for autism.

Aim To assess the relationship between the clinical sign of excessive hip abduction (abduction of the hip 90 degrees or more) and developmental dysplasia of the hip (DDH). Methods The research was conducted on 450 newborns in the Neonatal Unit at the Clinic of Gynecology and Obstetrics and the Clinic of Orthopedics and Traumatology of the University Clinical Centre, Tuzla, between 30th August 2011 and 30th April 2012. Clinical (degree of hip abduction) and ultrasound examination of all newborns hip were performed using the Graf method on their first day of life. Results Clinical sign of excessive hip abduction showed significant predictive value for DDH. There were 37 (8.2%) newborns with the clinical sign of excessive hip abduction, of which 13 of them had DDH while 24 of them did not have this clinical sign. There were 89 newborns, in the overall sample, with one of the forms of DDH, 13 (12.7%) of them had this clinical sign, while from 324 newborns witouth DDH, 24 (6.9%) had this clinical sign. Excessive hip abduction had a positive predictive value (PPV) of 35.1% and a negative predictive value (NPV) of 78.5% for DDH. Conclusion Excessive hip abduction is an useful and important clinical sign of DDH. Doctors who perform the first examination of the child after birth, would have to turn their attention to this clinical sign. The newborns with this clinical sign would have to go to an ultrasound examination of the hips for further diagnosis.

The aim of this paper is to examine the roles of the Islamic voluntary sector in two jurisdictions –one is representing an Asian country, Malaysia, and the other is the newest Member State of European Union, Croatia. The investigation undertaken in the research is purely library-based, engaging in secondary data including written rules, guidelines and reports in both jurisdictions. The preliminary findings reveal that there are numerous voluntary efforts being carried out by numerous organizations in both jurisdictions (Malaysia and Croatia), in sectors such as public finance, education, quality of life and health. The research further found that these efforts could potentially bring huge impacts to the development and well-being of the society, in four different dimensions: social, finance, organizational, and legal. This research will contribute to the body of knowledge on voluntary sectors especially on the roles and implications of the voluntary sectors towards shared prosperity of the people and economy in Malaysia and Croatia.