This work presents investigation of chemical composition and antioxidant activity of ethanolic extracts of leaves with flowers and berries prepared by ultrasound and Soxhlet extractions of Crataegus monogyna from Bosnia and Herzegovina. Total phenolic, flavonoid, and anthocyanin contents were measured by spectrophotometric methods. The sample of leaves with flowers extracted by Soxhlet extraction was the richest in the content of total phenolic compounds (14.43 mg GAE/g DW) and total flavonoids (2.22 mg QE/g DW). Same extract showed the best antioxidant activity with an IC50 value of 0.71 mg/mL for DPPH and 0.38 mg/mL for ABTS assay, as well as the highest content of gallic acid, caffeic acid, and hyperoside 0.04 mg GAE/g DW, 0.60 mg CA/g DW and 2.61 mg HYP/g DW, respectively, determined by HPLC-ED. Vitexin was not detected. The extract of berries obtained by ultrasound extraction had the highest amount of total anthocyanins (1.69 mg/100 g DW).

The spotted wing drosophila (SWD), Drosophila suzukii Matsumura (Diptera: Drosophilidae), is an invasive polyphagous pest of soft-skinned fruit that has started to threaten small fruit production in Europe since 2008. High reproductive capacity, short generation time, and difficulties in visualizing early damage contribute to its rapid spread. Currently, the control strategy against D. suzukii mostly relies on treatment with synthetic insecticides. Keeping in mind that this pest causes the greatest damage during the harvesting period, control using chemicals is not recommended due to the increased risk of high pesticide residue levels in the fruit. With the aim of reducing the use of insecticides, there is a need for developing an environmentally safer way of control. Alternative solutions could rely on the use of essential oils (EOs), which can be used in conventional and organic production systems. Four essential oils, geranium (Pelargonium graveolens), dill (Anethum graveolens), Scots pine (Pinus sylvestris), and bergamot (Citrus bergamia), were assessed for their insecticidal effects using four different tests (contact toxicity, fumigant-contact toxicity, repellent effect, and multiple-choice tests). The EOs applied were dissolved in acetone at three different concentrations. The most promising one was the geranium essential oil, which had the best effect in all conducted tests, even at the lowest applied concentration. Geranium oil caused very high mortality (95%), even at the lowest concentration applied, after 24 h in the fumigant-contact test, and it caused a mortality of over 85% in the contact test. It was also noticed that geranium demonstrated a deterrent effect by repelling females from laying eggs for four days after at the lowest applied concentration. Scots pine and dill EOs have moderate to strong effects in most tests. The mortality of 100% was achieved for the highest applied concentration in the fumigant-contact and contact tests. Bergamot EO did not have any significant insecticidal activity. Geranium, Scots pine, and dill have great potential to be used as an environmentally friendly way of controlling D. suzukii as they exhibit deterrent, repellent, and insecticidal effects.

Plant extracts are increasingly being examined in the corrosion inhibition of metal and alloys in various environments due to their potent antioxidant properties. The use of Artemisia annua L. aqueous extract (AAE) as an aluminium alloy 5083 (ALA) corrosion inhibitor in artificial seawater (ASW) was investigated using electrochemical tests and spectroscopy tools, while the active biocompounds found in AAE were analyzed using high-performance liquid chromatography (HPLC). Electrochemical results showed that AAE acts as an anodic inhibitor through the physisorption (ΔG ≈ –16.33 kJ mol−1) of extract molecules on the ALA surface, thus reducing the active sites for the dissolution of the alloy in ASW. Fourier-transform infrared spectra confirmed that phenolic acids found in AAE formed the surface layer that protects ALA against the corrosive marine environment, while HPLC analysis confirmed that the main phytoconstituents of AAE were chlorogenic acid and caffeic acid. The inhibition action of phenolic acids and their derivatives found in the AAE was based on the physisorption of caffeic acid on the ALA surface, which improved physicochemical properties of the barrier film and/or conversion of Al3+ to elemental aluminium by phenolic acids as reducens, which slowed down the diffusion rate of Al3+ to or from the ALA surfaces. The protective effect of the surface layer formed in the presence of AAE against ASW was also confirmed by inductively coupled plasma–optical emission spectrometry (ICP-OES) whereby the measured concentration of Al ions after 1 h of immersion of ALA in the pure ASW was 15.30 μg L−1 cm−2, while after the addition of 1 g L−1 AAE, the concentration was 3.09 μg L−1 cm−2.

Pyrophyllite was investigated in this study as a potential adsorbent for the removal of glyphosate, a common herbicide for the control of a wide variety of weeds. There are several publications in the literature that discuss how glyphosate interacts with clay minerals such as montmorillonite, kaolinite, and bentonite, but there is no information regarding the possibility of glyphosate removal from aqueous solution using pyrophyllite. In this study, the adsorption of glyphosate from water samples onto pyrophyllite, a mineral clay from the Parsovici, Konjic mine, was investigated. The ninhydrin reaction was used to spectrophotometrically quantify glyphosate. At 570 nm, the purple Ruhemman product's absorbance was determined. Adsorption capacity (qe), removal efficiency (R), and the partition coefficient are used to express the results (Kd). The outcomes demonstrated that distinct ionic species of glyphosate at different pHs cause the adsorption of glyphosate on pyrophyllite to be pH-dependent. The findings demonstrated that during different contact times, the basic medium can remove up to 75% of glyphosate. According to results from fitting isotherm models, the Freundlich model is slightly better fitted. According to the results of kinetic investigations, the pseudo-2nd order model is more suitable for this kind of adsorption.

Abstract Using HPLC-DAD the presence of catechin, epicatechin, quercetin, kaempferol and protocatehuic acid was analysed in herb extracts of eight Ephedra species (Ephedra sinica, Ephedra major, Ephedra chilensis, Ephedra ciliata, Ephedra foeminea, Ephedra equisetina, Ephedra gerardiana and Ephedra distachya). For comparison purposes, the same phenolics were determined in Camellia sinensis herb, which is a common source of catechins and a medicinal plant with valuable antioxidant properties. The catechin content in Ephedra spp. ranged between 0.83 mg/g d.w.– 8.77 mg/g d.w., while the epicatechin content was between 0.11 mg/g d.w.– 3.38 mg/g d.w. In comparison to black tea, Ephedra major, Ephedra distachya and Ephedra equisetina had a higher catechin content. The selected Ephedra spp. are rich in catechins and the obtained results serve as the chemical rationale for the anti-inflammatory, antioxidant and anti-proliferative activities of Ephedra that are currently being investigated. Graphical Abstract

In this study, the chemical composition and antioxidant activity of the hydrodistilled essential oil of Achillea lingulata, an endemic species of the Euro-Mediterranean region, originating from Bosnia and Herzegovina, was investigated for the first time. For comparison, an analysis of the essential oil of the widely distributed Achillea millefolium, which grows together in the same habitat, was made. Ninety-six components were identified in A. lingulata and A. millefolium oils comprising 97.8% and 85.8%, of the total oil, respectively. The oil of A. lingulata was characterized by a high content of oxygenated monoterpenes (76.8%). The main compounds were borneol (30.1%), trans-verbenol (15.5%), 2-tridecanone (12.2%), fragranol (8.3%), and myrtenol (7.9%). In contrast, essential oil of A. millefolium had oxygenated sesquiterpenes (60.8%) as the most abundant compounds, with elemol (32.9%) as the main constituent. In addition, γ-eudesmol (12.9%), caryophyllene oxide (7.7%), transcaryophyllene (5.7%) and γ-muurolene (4.7%) were present in a significant percentage in A. millefolium oil. Antioxidant activity was tested by three methods, ABTS, DPPH and FRAP, and the obtained results showed low activity of both investigated oils.

Biodiesel fuel is the realistic fuel for the future due to its environmental, economic and energetic benefits. However, the degradation and instability during biodiesel storage and application present the major disadvantages and hence a modern scientific challenge. The aim of this study was to choose optimal parameters for biodiesel synthesis from fresh and waste sunflower cooking oil and to investigate the possibilities of increasing its resistance towards oxidative degradation. Various physico-chemical characteristics essential for the quality estimation of the resulting biodiesel products were compared before testing the effectiveness of natural and synthetic antioxidants. Butylated hydroxytoluene (BHT), carvacrol and α-tocopherol were added to the biodiesel in different concentrations in order to determine their efficiency during the Schaal oven test. Results proved that both, fresh and waste oil can be valuable sources for the synthesis of biodiesel that meets European and American quality standards. Among the antioxidants, BHT was the most efficient one in both types of biodiesel and its usage would be recommended at the concentration of 1000 ppm. The findings present a cost-effective and environmentally friendly source for biodiesel production with improved properties - considerably enhanced resistance to oxidative degradation, where synthetic antioxidants are given the priority. DOI: http://dx.doi.org/10.17807/orbital.v10i7.1174

The aim of this work was the qualitative and quantitative determination of selected phenolic compounds in three Crataegus species grown in Bosnia. Crataegus plants are consumed for medicinal purposes and as foodstuff in the form of canned fruit, jam, jelly, tea, and wine. Two samples of plant material, dry leaves with flowers, and berries of three Crataegus species—Crataegus rhipidophylla Gand., Crataegus x subsphaericea Gand., and Crataegus x macrocarpa Hegetschw.—were analyzed. Twelve ethanolic extracts were isolated from the selected plant material using Soxhlet and ultrasound extraction, respectively. Soxhlet extraction proved to be more effective than ultrasound extraction. A simple and sensitive method, high-performance liquid chromatography with electrochemical detection, HPLC-ED, was used for the simultaneous determination of phenolic acids and flavonoids in Crataegus species. The content of gallic acid in the extracts ranged from 0.001 to 0.082 mg/g dry weight (DW), chlorogenic acid from 0.19 to 8.70 mg/g DW, and rutin from 0.03 to 13.49 mg/g DW. Two flavonoids, vitexin and hyperoside, commonly found in chemotaxonomic investigations of Crataegus species, were not detected in the examined extracts. In general, leaves with flowers samples are richer in gallic acid and rutin, whereas the berries samples are richer in chlorogenic acid. Distinct similarities were found in the relative distribution of gallic acid among the three species. Extracts of C. x macrocarpa had the highest content of all detected compounds, while significant differences were found in rutin content, depending on the plant organ. To the best of our knowledge, this is the first study reporting content of phenolic compounds in Crataegus rhipidophylla Gand., Crataegus x subsphaericea, and Crataegus x macrocarpa from Bosnia.