The proper application of retrosynthesis to identify possible transformations for a given target compound requires a lot of chemistry knowledge and experience. However, because the complexity of this technique scales together with the complexity of the target, efficient application on compounds with intricate molecular structures becomes almost impossible for human chemists. The idea of using computers in such situations has existed for a long time, but the accuracy was not sufficient for practical applications. Nevertheless, with the steady improvement of machine learning and artificial intelligence in recent years, computer-assisted retrosynthesis has been gaining research attention again. Because of the overall lack of chemical reaction data, the main challenge for the recent retrosynthesis methods is low exploration ability during the analysis of target and intermediate compounds. The main goal of this research is to develop a novel, template-free approach to address this issue. Only individual molecular substructures of the target are used to determine potential disconnection sites, without relying on additional information such as chemical reaction class. The model for the identification of potential disconnection sites is trained on novel molecular substructure fingerprint representations. For each of the disconnections suggested using the model, a simple structural similarity-based reactant retrieval and scoring method is applied, and the suggestions are completed. This method achieves 47.2% top-1 accuracy for the single-step retrosynthesis task on the processed United States Patent Office dataset. Furthermore, if the predicted reaction class is used to narrow down the reactant candidate search space, the performance is improved to 61.4% top-1 accuracy.

The research dealt with the identification of significant differences in the basic dimensions of personality according to the five-factor model between two groups of respondents: heroin addicts and the control group of respondents, non-consumers of psychoactive substances. Also, we wanted to determine whether the respondents - heroin addicts in relation to the non-clinical group differ significantly in personality traits given the completeness of the family environment in which they grew up, and the propensity of parents to alcoholism and violent behavior. The study included a sample of 297 subjects, of which 155 were heroin users, and 142 were non-clinical control group subjects, students of different study profiles of the University of Tuzla. The clinical and non-clinical samples were uniform in terms of characteristics important for the research. In order to test the research hypotheses, in addition to the insight into the descriptive parameters, a one-way analysis of variance was used. The results show that heroin users achieve significantly higher results in the dimensions of Agreeableness (F = 21.51; p = 0.00), Conscientiousness (F = 13.89; p = 0.00) and Neuroticism (F = 13.23; p = 0.00), while the clinical and non-clinical groups do not differ significantly in the dimensions of Openness and Extraversion. In the group of heroin consumers, respondents coming from incomplete families achieve significantly lower results on the Conscientiousness dimension (F = 4.20; p = 0.04), the tendency to consume parental alcohol proved to be a significant factor for the Neuroticism dimension (F = 4.39; p = 0, 04) and Openness (F = 15.41; p = 0.00), while parental propensity to violent behavior proved to be a significant factor for the Neuroticism dimension (F = 4.49; p = 0.04) and Openness (F = 15.41; p = 0.00). ). When it comes to the non-clinical control group, family completeness and characteristics of the family environment (parents ‘tendency to consume alcohol and parents’ tendency to violent behavior) did not prove to be significant factors for differences in personality traits.

Generation Z falls into the internet generation because it is the first generation born after the popularisation of the internet. Even though scientists don’t agree on when this generation starts and when it ends, after all, it is believed that the generation Z is made of people that are born in the nineties until today. Their parents belong to the millennial generation that is born in the eighties. Generation Z is the first generation that was raised as a part of the trend of being in the publics' eye, always posting about their lives on social networks. This potentially makes them an egocentric generation and a generation that does not have enough empathy. Although empathy is one of the key characteristics for happiness, generation Z finds happiness in their personal appearance and they care a lot about the opinions of others, that is, they care about the number of likes and the number of followers on social networks. Even though empathy is a key trait of happiness, generation Z finds happiness in physical appearance, and in others' opinion, that is they care about how many likes and how many followers on social networks they have. The purpose of this paper is to examine emotional empathy with the generation that is born in the late nineties, until generations 2006-2007, on a sample of 200 primary and high school students with an assumption that later generations are more empathic and that there is a difference between male and female subjects, that is that females are more empathic. Also, the paper provides case reports that represent the clinical population and not the average representatives of this generation and whose psychological problems may be associated with excessive use of the Internet and social networks. The results of the research indicate that the feeling of insecurity and lack of empathy in this generation is a risk factor for the development of low self-esteem and self-confidence, and ultimately more severe mental disorders, which can be reduced and prevented by a different approach to this generation.

Uncertainty, unfolding in the most extreme forms in the period of one of the greatest global challenges – a threat to the health and wellbeing of people, caused by the pandemic, is gaining a form difficult to conceive in temporal and conceptual terms. In the perception of students, the young people on the brink of the first employment, the uncertainty primarily reflects in the estimate of possibilities of finding a job and opportunities to construct a professional identity. This paper examines the potential role of personal branding in the improvement of student opportunities to find the desired job, as well as the importance of higher education institutions as strategic partners in the process. This paper, among others, also emphasizes the importance of empowering students towards the recognition of the role of their knowledge and competencies acquired during the studies; the role which does not correspond to banal, oversimplified reduction of the role of education as means to meet the labour market needs.

This paper presents the results of a preliminary study, which utilizes a hashtag-based approach and mixed-method procedures to describe the nexus between commemoration practices and visual social media. It mainly focuses on Instagram hashtags for the Day of the Republic of Srpska, a disputed and controversial public holiday celebrated on the 9th of January in the Republic of Srpska, to track how commemoration practices related to national memories are shaped through the process of digital (re)mediation.

Anti-aging cosmetics are often sought after in order to slow down the aging process. Free radicals are one of the main causes of skin aging, and therefore antioxidants are used in anti-aging cosmetics. The aim of this study was to investigate which method is the most suitable for determining the antioxidant capacity of these products. Having samples extracted, the antioxidant capacity of the extracts obtained was determined by the following spectrophotometric methods: DPPH, Folin-Ciocalteu, FRAP, the ABTS method and the ferroion chelation method with ferrosine. The antioxidant capacity of the samples varied depending on the extract type and the method used. DPPH and ferroion chelation measurements with ferrosine were carried out in the part of the spectrum where plant pigments absorb. These pigments are often found in anti-aging products affecting these methods measurement results. The Folin-Ciocalteu method is suitable for researching the antioxidant capacity of hydrophilic extracts, but not lipophilic ones, where turbidity and the formation of a gelled ring occur. The FRAP method revealed similar results for all the samples and proved to be less sensitive than the others. The ABTS method for both types of extracts has proven to be the most suitable and sensitive method for determining the antioxidant capacity of anti-aging products.

A system of fifteen variables (7 morphological and 8 variables for assessing postural status) was applied to a sample of 284 male and female students from the first to the third grade of primary school, with the aim of determining the differences between boys and girls. The results showed that there were statistically significant differences between the examined samples in the following morphological variables: in the first class (body weight and skin fold of the triceps), in the second class (body height, body weight, skin fold of the triceps and skin fold of the biceps) and in third grade (body height, skin fold of triceps, skin fold of back, skin fold of biceps, and skin fold of lower leg). Statistically significant differences in the variables for assessing postural status exist in the following variables: in the first grade (head posture, shoulder posture, shoulder posture, chest shape and leg shape), in the second grade (shoulder posture, chest shape, and spinal deviation in frontal plane) and in the third grade (deviation of the spinal column in the frontal plane, holding the anterior abdominal wall and the shape of the legs). Based on the obtained results, it can be concluded that in the younger school-age in morphology there is a significant sexual dimorphism and with it in connection with the change of postural status.

A method of encryption of the 3D plane in Geographic Information Systems (GIS) is presented. The method is derived using Voronoi-Delaunay triangulation and properties of Catalan numbers. The Voronoi-Delaunay incremental algorithm is presented as one of the most commonly used triangulation techniques for random point selection. In accordance with the multiple applications of Catalan numbers in solving combinatorial problems and their "bit-balanced" characteristic, the process of encrypting and decrypting the coordinates of points using the Lattice Path method (walk on the integer lattice) or LIFO model is given. The triangulation of the plane started using decimal coordinates of a set of given planar points. Afterward, the resulting decimal values of the coordinates are converted to corresponding binary records and the encryption process starts by a random selection of the Catalan key according to the LIFO model. These binary coordinates are again converted into their original decimal values, which enables the process of encrypted triangulation. The original triangulation of the plane can be generated by restarting the triangulation algorithm. Due to its exceptional efficiency in terms of launching programs on various computer architectures and operating systems, Java programming language enables an efficient implementation of our method.

In this letter we present a fast triple voltage quenching circuit (TVQC) with an integrated <inline-formula> <tex-math notation="LaTeX">$40~\mu \text{m}$ </tex-math></inline-formula> diameter single-photon avalanche diode (SPAD) in 0.35-<inline-formula> <tex-math notation="LaTeX">$\mu \text{m}$ </tex-math></inline-formula> CMOS. By pre-biasing the switching MOSFETs the reaction time of the TVQC is kept small, leading to a total quenching time of only 1.4 ns of which 0.61 ns are actively quenched. This short reaction time reduces the avalanche charge and therefore also the afterpulsing probability (APP). The dead time is adjustable from 7.9 ns to 200 ns, which allows further reducing the APP. Experimental verification shows an APP of 2.1% at a dead time of 30 ns. Using an integrated SPAD with a thick absorption zone allows achieving a photon detection probability (PDP) of 28.8% at 850 nm, while showing a peak PDP of 53.1% at 657 nm, both at 9.9 V excess bias.

Aim To assess concordance of eight frequently used serology-based scoring indices for liver fibrosis and cirrhosis with transient elastography (TE) in chronic hepatitis C (CHC) patients in order to determine serum indices with the highest concordance and clinical usability in clinical practice. Methods In this prospective study, 63 CHC patients were included and TE results were compared with eight non-invasive indices. The diagnostic performance of these tests was assessed using receiver operating characteristic curves with kappa index calculated for the concordance analysis. Results Median age of 63 patients was 54 years (interquartile range: 42 to 63); 27 (42.9%) were females. According to areas under the Receiver Operating Characteristics (AUROC), the best performing serum markers for significant liver fibrosis (METAVIR ≥F2), advanced liver fibrosis (≥F3) and cirrhosis (F4) determined by TE measurements (≥7.1kPa, ≥9.5kPa and ≥12kPa, respectively) were Fibrotest (AUROC=0.727 for ≥F2) and FIB-4 score (AUROC=0.779 for ≥F3 and AUROC=0.889 for F4). Fibrotest cut-off at >0.50 was concordant with TE for presence of significant fibrosis in 30 (out of 45; 66.7%), FIB-4 cut-off at <1.45 was concordant for absence of significant fibrosis in 13 (out of 18; 72.2%) and Goeteborg University Cirrhosis Index (GUCI) cut-off at >1 was concordant for presence of cirrhosis in 16 (out of 22; 72.7%) patients, but not for exclusion of cirrhosis. Conclusion Serology-based scoring indices had moderate overall concordance with TE. We propose that FIB-4 score, Fibrotest and GUCI be used in routine practice to exclude and diagnose significant fibrosis and diagnose cirrhosis, respectively.

Immunosuppressive drugs are administered to decrease immune system activity (e.g. of patients undergoing solid organ transplant). Concentrations of immunosuppressive drugs (ISDs) in circulating blood must be closely monitored during the period of immunosuppression therapy due to adverse effects that take place when concentration levels fall outside of the very narrow therapeutic concentration range of these drugs. This study presents the rapid determination of four relevant immunosuppressive drugs (tacrolimus, sirolimus, everolimus, and cyclosporine A) in whole human blood by directly coupling solid-phase microextraction to mass spectrometry via the microfluidic open interface (Bio-SPME-MOI-MS/MS). The BioSPME-MOI-MS/MS method offers ≤ 10% imprecision of in-house prepared quality controls over a 10-day period, ≤ 10% imprecision of ClinCal® Recipe calibrators over a three-day period, and single total turnaround time of ∼ 60 min (4.5 min for high throughput). The limits of quantification were determined to be 0.8 ng mL-1 for tacrolimus, 0.7 ng mL-1 sirolimus, 1.0 ng mL-1 for everolimus, and 0.8 ng mL-1 for cyclosporine. The limits of detection were determined to be 0.3 ng mL-1 for tacrolimus, 0.2 ng mL-1 for sirolimus, 0.3 ng mL-1 for everolimus, and 0.3 ng mL-1 for cyclosporine A. The R2 values for all analytes were above 0.9992 with linear dynamic range from 1.0 mL-1 to 50.0 ng mL-1 for tacrolimus, sirolimus, and everolimus while from 2.5 ng mL-1 to 500.0 ng mL-1 for cyclosporine A. To further evaluate the performance of the present method, 95 residual whole blood samples of tacrolimus and sirolimus from patients undergoing immunosuppression therapy were used to compare the Bio-SPME-MOI-MS/MS method against a clinically validated reference method based on chemiluminescent microparticle immunoassay, showing acceptable results. Our results demonstrated that Bio-SPME-MOI-MS/MS can be considered as a suitable alternative to existing methods for the determination of immunosuppressive drugs in whole blood providing faster analysis, better selectivity and sensitivity, and a wider dynamic range than current existing approaches.

Balneo-gynaecological treatment methods include external bath hydrotherapy, sedentary baths and topical dressings/cataplasm, and internal (intravaginal or intrarectal use of peloids and mineral water). Hyperosmolar thermal spas have been very popular in the treatment of infertility due to the improvement of symptoms of chronic pelvic pain, endometriosis, chronic vascular and inflammatory pelvic diseases. Acute pelvic inflammatory syndrome is a contraindication for balneo-hydrotherapy while hyperthermal hydrotherapy is contraindicated in endometriosis and neurovegetative dystonia due to the stimulation of hyperemia, which worsens the clinical picture. Balneo-hydrotherapy is not recommended in metrorrhagia and malignancies. Balneogynaecological treatment certainly has its own primary but also complementary role in the treatment of chronic gynaecological diseases and is increasingly recommended today.

ABSTRACT Six blood groups (Rh, MN, Duffy, Kidd, Kell, and Lutheran) were investigated among three major ethnic groups (Bosniaks, Bosnian Croats, and Bosnian Serbs), as well as 10 regional subpopulations across Bosnia and Herzegovina (B&H): Krajina; Posavina; northeastern, eastern, middle, and central Bosnia; Sarajevo region; eastern, central, and western Herzegovina. This is the first study that introduces the molecular genetic typing of five blood groups within the B&H population, with the exception of the RhD blood group. The sample consisted of 450 buccal swabs from unrelated individuals. Five blood group systems (RhD, RhC, RhE, Kidd, MN) were genotyped by PCR with sequence specific primers, while three blood group systems (Kell, Duffy, Lutheran) were genotyped by the PCR-restriction-fragment-length polymorphism method. Minor variation of genetic diversity was observed within the three major B&H ethnic groups, as well as within the 10 subpopulations stratified according to geographical criteria. No genetic differentiation among ethnic groups was noticed. These results are in agreement with the results of previous studies based on different molecular genetics markers, which indicate that the three B&H ethnic groups belong to the same gene pool. A similar level of genetic variance was observed within regional subpopulations, with no significant genetic differentiation among them. Comparison of intrapopulation genetic diversity of the B&H population with other European and non-European populations, based on three loci (RHD, MN, and KEL), clearly show that the level of genetic diversity of the B&H population is within the European range.