Cilj ovog rada bio je ispitati utjecaj termičke obrade kuhanjem na sadržaj željeza (Fe), cinka (Zn), bakra (Cu) i magnezija (Mg) u mišićnom tkivu i jetri junadi u ovisnosti od sustava tova iz kojeg životinje potječu. Istraživanje je provedeno na uzorcima mišićnog tkiva (but i plećka) i jetre junadi u dobi od 9 do 12 mjeseci koja su tovljena ekstenzivno (n=12) ili intenzivno (n=12). Svaki uzorak je podijeljen na dva jednaka dijela od kojih je jedan analiziran u sirovom stanju, a drugi nakon termičke obrade kuhanjem u vodi na 100°C. Rezultati su iskazani u apsolutno suhoj tvari mesa. Sadržaj pepela (mineralnog ostataka) u termički obrađenim uzorcima bio je smanjen u odnosu na sirove uzorke, ali to nije bilo praćeno značajmeso junadi, termička obrada, minerali nim promjenama u sadržaju Fe, Zn i Cu u mišićnom tkivu, niti promjenama Zn i Cu u jetri. Termička obrada dovela je do smanjenja sadržaja Mg u svim ispitivanim partijama mesa (za 40-50%), a do smanjenja sadržaja Fe došlo je samo u jetri (za 25-42 %). Učinak termičke obrade na sadržaj ispitivanih minerala u mišićnom tkivu i jetri bio je neovisan od sustava tova, iako su razlike po sustavu tova bile evidentne kod svih ispitivanih minerala, osim kod Fe. Sadržaj Zn u mišićnom tkivu buta junadi iz ekstenzivnog tova bio je manji, a sadržaj Cu veći u odnosu na uzorke buta iz intenzivnog sustava tova. Suprotno od buta, u jetri je sadržaj Cu bio veći kod junadi iz intenzivnog tova. U svim ispitivanim partijama mesa iz ekstenzivnog tova sadržaj Mg je bio značajno veći nego kod junadi iz intenzivnog tova.

A graph convolutional network (GCN) is employed in the deep energy method (DEM) model to solve the momentum balance equation in three‐dimensional space for the deformation of linear elastic and hyperelastic materials due to its ability to handle irregular domains over the traditional DEM method based on a multilayer perceptron (MLP) network. The method's accuracy and solution time are compared to the DEM model based on a MLP network. We demonstrate that the GCN‐based model delivers similar accuracy while having a shorter run time through numerical examples. Two different spatial gradient computation techniques, one based on automatic differentiation (AD) and the other based on shape function (SF) gradients, are also accessed. We provide a simple example to demonstrate the strain localization instability associated with the AD‐based gradient computation and show that the instability exists in more general cases by four numerical examples. The SF‐based gradient computation is shown to be more robust and delivers an accurate solution even at severe deformations. Therefore, the combination of the GCN‐based DEM model and SF‐based gradient computation is potentially a promising candidate for solving problems involving severe material and geometric nonlinearities.

Neprekidni rast broja stanovnika u urbanim područjima ograničavajući je faktor u uzgoju bilja u gradskim i van gradskim područjima, a rješenje se može pronaći u urbanoj poljoprivredi. Budući da se hortiterapija veoma često provodi u urbanoj sredini logično je da predstavlja oblik urbane poljoprivrede. Pored značaja urbane poljoprivrede u radu se razmatra i nivo (udio) njene zastupljenosti. Kroz cilj rada utvrđen je i značaj hortiterapije u liječenju. Cilj rada bio je istražiti koliko su građani Mostara upoznati s urbanom poljoprivredom i hortiterapijom te dobrobitima koje ona pruža. Rezultat su pokazali da građani Mostara imaju tek dovoljna do osrednja znanja o urbanoj poljoprivredi i hortiterapiji. Mišljenja o zadovoljavajućoj zastupljenosti uzgoja poljoprivrednih kultura i ukrasnog bilja u privatnim dvorištima, gradskim zelenim površinama, gradskim vrtovima i slično propagira većina ispitanika. Najveći broj ispitanika samostalno, ali tek ponekad u okviru obiteljskih, gradskih vrtova te balkona i terasa uzgaja različite poljoprivredne kulture i ukrasno bilje. Građani se u većoj mjeri nezadovoljavaju s gospodarskom koristi od uzgoja bilja u urbanoj sredini. Rad s biljkama na njih ima pozitivne zdravstveno-psihičke uticaje u zadovoljavajućoj mjeri. Također, više od polovine ispitanika posjeduje stav kako urbana poljoprivreda ipak pozitivno utiče na poboljšanje društvenih odnosa u Mostaru. Većina ispitanika uzgaja bilje po ekološkim principima u zadovoljavajućoj mjeri. Razvojem urbane poljoprivrede i hortiterapije poboljšava se zdravstveno-psihičko stanje pojedinaca te se unapređuje gospodarsko stanje, društveni odnosi, okoliš i slično.

Purpose Treatment of distal vessel occlusions causing incomplete reperfusion after mechanical thrombectomy (MT) is debated. We hypothesized that pretreatment with intravenous thrombolysis (IVT) may facilitate delayed reperfusion (DR) of residual vessel occlusions causing incomplete reperfusion after MT. Methods Retrospective analysis of patients with incomplete reperfusion after MT, defined as extended thrombolysis in cerebral infarction (eTICI) 2a–2c, and available perfusion follow-up imaging at 24 ± 12 h after MT. DR was defined as absence of any perfusion deficit on time-sensitive perfusion maps, indicating the absence of any residual occlusion. The association of IVT with the occurrence of DR was evaluated using a logistic regression analysis adjusted for confounders. Sensitivity analyses based on IVT timing (time between IVT start and the occurrence incomplete reperfusion following MT) were performed. Results In 368 included patients (median age 73.7 years, 51.1% female), DR occurred in 225 (61.1%). Atrial fibrillation, higher eTICI grade, better collateral status and longer intervention-to-follow-up time were all associated with DR. IVT did not show an association with the occurrence of DR (aOR 0.80, 95% CI 0.44–1.46, even in time-sensitive strata, aOR 2.28 [95% CI 0.65–9.23] and aOR 1.53 [95% CI 0.52–4.73] for IVT to incomplete reperfusion following MT timing <80 and <100 min, respectively). Conclusion A DR occurred in 60% of patients with incomplete MT at ~24 h and did not seem to occur more often in patients receiving pretreatment IVT. Further research on potential associations of IVT and DR after MT is required.

Corpus-based activities (CBAs) have been used in FLT in recent decades, especially in countries where educational systems are well developed. However, this is not the case in Bosnia and Herzegovina. That is why a case study was conducted in a primary school in Bosnia and Herzegovina including 48 7th grade students divided into an experimental and a control group to investigate the effect of the use of corpus-based activities on vocabulary acquisition. In both groups blended learning methods were applied due to COVID restrictions. The aim was also to determine whether the use of CBAs had any impact on the students' motivation for participating in vocabulary learning activities. The students' results achieved in the pre- and post-tests which included new vocabulary items were analyzed and compared. The students' responses in the five-point Likert scale motivation questionnaires completed prior to and after the use of CBAs were also compared and discussed. Statistical analysis of the results confirmed that there was a positive effect of the use of CBAs on vocabulary acquisition of A1 – A2 level seventh graders, as well as on their motivation for participating in such activities. Contrary to statistically significant improvement in vocabulary acquisition, there is no statistically significant increase in the motivation to learn new vocabulary items in either of the groups, for which some possible explanations are also discussed in the paper.  Article visualizations:

Body mass index (BMI) and body image (BI) are constructs worth examining in the context of physical activity (PA), and they are both related to quality of life (QoL). PA, BMI, and BI should all be considered as associated parameters, and their effect on QoL should be examined and understood. This study aimed to determine the moderating role of PA in the relationship of BMI and BI with QoL. The sample of examinees consisted of 500 respondents (307 women; aged 39 ± 6 years). A physical activity self-evaluation questionnaire (IPAQ-SF) was used to estimate PA; BI was evaluated by using the Body Image Dimensional Assessment (BIDA) questionnaire, while QoL was determined with the WHOQOL-BREF questionnaire. The results showed that vigorous PA moderates the relationship between BMI and social relationships in adults (Sig. = 0.000). Walking and vigorous PA affect the relationship between BMI and environmental health (Sig. = 0.017 and Sig. = 0.049, respectively). Both walking (Sig. = 0.035) and moderate PA (Sig. = 0.032) alternate the relationships between BI and social relationships. Walking (Sig. = 0.000) and vigorous PA (Sig. = 0.016) moderate the relationship between BI and environmental health. The influence of PA on the moderation of the relationship of BMI and BI with physical and psychological health in the working population was not statistically significant.

Skin secretion of amphibians often represents the only weapon of these species against pathogens and predators. Peptides constitute the major portion of active molecules of that weapon and may be treated as potential pharmaceuticals for future generations. The first step of their efficient use involves establishing of their primary structure, i.e., sequencing. De novo sequencing by means of mass spectrometry was applied to Rana arvalis species, collected in the spring 2021 in Central Slovenia (vicinity of Ljubljana). HPLC-ESI-HRMS/MS with Orbitrap instruments was used to establish the skin peptidome of these species and compare it with the earlier identified skin peptidome of the Moscow population of Rana arvalis. Application of CID, HCD, ETD, and EThcD enabled detecting and sequencing 18 peptides; five of them were novel and may be treated as possible biomarkers of the Ljubljana population of Rana arvalis. Interestingly, representatives of two peptide families (temporins and brevinins 2) were not found in the Moscow population. MS3 modes, first of all EThcD, demonstrated their great potential in the de novo sequencing, including extraction of the sequence information from the intact peptides with disulfide cycle (rana box) in their structure and differentiation of isomeric Leu/Ile residues. Thus, all six isomeric residues were reliably distinguished in the novel melittin-related peptide AK-23-1. In addition, another post-translational modification dealing with carbonylation of the N-terminal Gly of novel temporin AVa was established using the MS3 mode. The obtained results demonstrate the efficiency of the use of MS3 tools in proteomics/peptidomics.

In order to best conserve, as well as utilize, traditional apple germplasm in Norway, an apple heritage cultivar collection was established in Ullensvang, western Norway, which aims to become the National Clonal Germplasm Repository. The establishment of the apple heritage cultivar collection was preceded by a molecular study that aimed to genotype a large number of apple accessions maintained in various ex situ sites in western and south-eastern Norway, using a rather small set of eight SSR markers. However limited, the marker set managed to identify synonyms, homonyms, and duplicates within and among the investigated collections. In this study, 171 apple accessions from the Ullensvang apple heritage cultivar collection were genotyped using a set of 20 different SSR markers. Approximately half of the accessions have been previously genotyped using eight SSR markers, enabling an assessment of whether the use of a larger marker set would yield a more accurate characterization. Based on the obtained molecular data, the apple heritage cultivar collection was determined to hold a key part of the overall genetic diversity of the Norwegian apple germplasm. Furthermore, the twelve additional SSR markers were able to differentiate several accessions groups originally thought to be synonyms, as well as to provide a more detailed insight into the genetic structure of this germplasm.

Introduction In one third of all patients with epilepsy, seizure freedom is not achieved through anti-seizure medication (ASM). These patients have an increased risk of earlier death, poorer cognitive development, and reduced quality of life. Cenobamate (CNB) has recently been approved as a promising novel ASM drug for the treatment of adults with focal-onset epilepsy. However, there is little experience for its application in pediatric patients. Methods In a multicenter study we evaluated retrospectively the outcome of 16 pediatric patients treated “off label” with CNB. Results In 16 patients with a mean age of 15.38 years, CNB was started at an age of 15.05 years due to DRE. Prior to initiation of therapy, an average of 10.56 (range 3–20) ASM were prescribed. At initiation, patients were taking 2.63 (range 1–4) ASM. CNB was increased by 0.47 ± 0.27mg/kg/d every 2 weeks with a mean maximum dosage of 3.1 mg/kg/d (range 0.89–7) and total daily dose of 182.81 mg (range 50–400 mg). Seizure freedom was achieved in 31.3% and a significant seizure reduction of >50% in 37.5%. Adverse events occurred in 10 patients with fatigue/somnolence as the most common. CNB is taken with high adherence in all but three patients with a median follow-up of 168.5 days Conclusion Cenobamate is an effective ASM for pediatric patients suffering from drug-resistant epilepsy. In addition to excellent seizure reduction or freedom, it is well-tolerated. Cenobamate should be considered as a novel treatment for DRE in pediatric patients.

It is well established that academic performance (AP) depends on a number of factors, such as intellectual capacities, practice, and previous knowledge. We know little about how these factors interact as they are rarely measured simultaneously. Here we present mediated-Factors of Academic Performance (m-FAP) model, which simultaneously assesses direct and indirect, mediated, effects on AP. In a semester-long study with 118 first-year college students, we show that intelligence and working memory only indirectly influenced AP on a familiar, less challenging college course (Introduction to Psychology). Their influence was mediated through previous knowledge and self-regulated learning activities akin to deliberate practice. In a novel and more challenging course (Statistics in Psychology), intellectual capacities influenced performance both directly and indirectly through previous knowledge. The influence of deliberate practice, however, was considerably weaker in the novel course. The amount of time and effort that the students spent on the more difficult course could not offset the advantage of their more intelligent and more knowledgeable peers. The m–FAP model explains previous contradictory results by providing a framework for understanding the extent and limitations of individual factors in AP, which depend not only on each other, but also on the learning context.