OBJECTIVE Canine hip dysplasia (CHD) is a global disease and an improved diagnostic approach to this orthopedic condition is needed, with an emphasis on inexpensive and minimally invasive techniques. This research aimed to determine the differentiating potential of selected biochemical markers in serum between healthy dogs and dogs with hip dysplasia in a breed-specific study that involved the Tornjak dog population. ANIMALS 99 Tornjak dogs radiographically categorized (Federation Cynologique Internationale procedure and scoring scheme) between December 2019 and April 2021, as having no sign of hip dysplasia or near normal hip joints (nondysplastic group; n = 51) vs mild, moderate, or severe hip dysplasia (dysplastic group; 48). METHODS Serum concentrations and enzyme activity of the biochemical markers hyaluronic acid (HA), procollagen II C-terminal propeptide (PIICP), and metalloproteinase 9 (MMP9) were compared among adult dogs with or without radiographic hip dysplasia. Statistical tests used to examine the differentiating potential of biochemical markers in Tornjak dog groups were assessed using the Mann-Whitney U test, logistic regression, and receiving operating characteristics (ROC) analysis. RESULTS Tornjak dogs with radiographic CHD had significantly lower serum concentrations of HA and higher concentrations of PIICP and MMP9 activity compared to dogs with radiographically normal hips (P < .05). Selected biochemical markers could distinguish dogs with radiographic CHD from those without CHD with high sensitivity and specificity. CLINICAL RELEVANCE These data suggest that the diagnostic technique of measuring serum concentrations of HA, PIICP, and MMP9 activity has a selective ability to distinguish dogs with dysplastic from dogs with normal hips.

The infection with SARS-CoV-2 virus in cats and dogs raised issue of human-to-animal transmission of SARS-CoV-2 in domestic pets in close contacts with their owners. Our study was designed to research this in the framework of Bosnia and Herzegovina. Using ELISA, AFIAS fluorescent immunoassay, RT-qPCR and WGS on Nanopore MinION platform with ARTIC Network Amplicon sequencing protocol for SARS-CoV-2, we showed that three out of thirteen dogs and one out of five cats from the households with confirmed human cases of COVID-19 in Bosnia-Herzegovina were infected with SARS-CoV-2. The high viral RNA load was detected in samples collected from a 4-year-old male Havanese (Ct = 12.52), a 6-year-old German Shepherd (Ct = 21.36) and a 9-year-old female American Staffordshire terrier (Ct = 25.74). The antibody response in dogs and one cat was observed. The viral genetic sequences from dogs were identical to the sequences detected in the owners suggesting the human-to-animal transmission of the virus. These findings, especially the low initial Ct values detected, from the public health perspective additionally stress the need for precautionary measures to protect both humans and animals.

The exposure of the body to stress, regardless of whether it comes from physical, chemical or emotional stimuli from the environment, causes an inadequate adaptation of the organisms which can contribute to the development of various diseases. Abnormally high blood concentrations of cortisol, known as stress hormone, lead to the development of a hormonal disorder called hyperadrenocorticism or Cushing’s syndrome. In the majority of cases, Cushing’s syndrome is diagnosed when symptoms are apparent, and screening endocrinological test confirms the existence either of increased cortisol production or decreased sensitivity of the hypothalamic-pituitary-adrenal axis to negative glucocorticoid feedback. In our research, we examined a total of 23 male and 7 female dogs that were suspected to have Cushing’s syndrome, based on history and clinical signs. A total of 15 male and 5 female dogs were positive for Cushing’s syndrome (HAC group), whereas the remaining dogs were used to form non-HAC group. Using the apparatus IDEXX “Vet Test 8008”, the following biochemical parameters were determined: glucose, urea, creatinine, phosphorus, calcium, total protein, albumin, globulin, alanine aminotransferase, alkaline phosphatase, bilirubin, cholesterol, and amylase. Regarding haematological parameters, the following parameters were investigated: erythrocytes, leukocytes, platelets, erythrocyte indices (MCV, MCH, MCHC, RDW), white blood cell count, haemoglobin and haematocrit, using “Laser cite vet lab Station” (IDEXX). No significant differences in haematological and biochemical blood parameters were noticed between the HAC and the non-HAC group of dogs. However, dogs suffering from Cushing’s syndrome had a higher value in the number of erythrocytes compared to the control group. The finding that has to be payed attention to is the difference in platelet count between the control group of dogs and dogs suffering from Cushing’s syndrome.

Introduction: The technique of closing the appendiceal stump using linear titanium stapler clips is being used more and more frequently in laparoscopic surgery, despite the good practice in the use of resorbable single endoloop vicryl ligatures and/or the non-resorbable plastic hem-o-lok clips. No light has been shed on potential undesirable effects on the haemathological profile of the use of titanium stapler clips. This experimental study aimed at establishing any possible undesirable effect of linear titanium stapler clips and single resorbable vicryl endoloop ligatures on the blood cells in rats following appendectomy. Methods: A total of 70 adult rats were used, divided into a control group (n = 10), and two experimental groups (n = 30 + 30). The appendices were removed from the rats in the first experimental group using titanium stapler clips and in the second experimental group the appendiceal stump using resorbable endoloop vicryl ligatures was closed. In both experimental groups three sub-groups with ten animals each were formed, from which peripheral blood from a tail vein on days 7, 28 and 60 was removed postoperatively, in order to assess the usual haematological parameters. Stained blood smears were also analysed in order to establish any poikilocytotic erythrocytes present. Results: In the first experimental group, with the titanium stapler clips, more than 25 % neutrophils were found on day 7, which is a significantly different result (p < 0.05) to the control group. In the second experimental group, there were more neutrophils than in the titanium stapler clips group, especially on days 7 and 60 and the results of these two sub-groups differ statistically significantly, p < 0.05. Hypochromia was found in the endoloop vicryl ligatures group, as well as in the titanium stapler clips sub-group on day 28, due to lower haemoglobin values which were significantly different to the control group, p < 0.05. Moderate levels of annulocytes, spherocytes and stomatocytes were found in most experimental groups. Conclusion: The results of this study favour the use of linear titanium stapler clips over resorbable single endoloop vicryl ligatures, because a less unfavourable effect was established on the blood cells of the experimental rats with their use.

There are only a few reported cases of abdominal ectopic pregnancy and consequential foetal mummification in dogs. The majority of the described cases indicate that this condition may exist for a prolonged period of time without any manifestation of illness. Therefore, sudden signs of abdominal distension, abdominal pain, episodes of epileptiform seizures, dark diarrhoea and lethargy, as were observed in the present case, are not likely to be considered as symptoms of an abdominal ectopic pregnancy. Furthermore, to the best of our knowledge, this is the first report of an eight-year long abdominal pregnancy in an ovariohysterectomised bitch. We present the clinical, laboratory, radiological and intraoperative findings, and the medical and surgical therapy for an abdominal ectopic pregnancy in a 14 year old Miniature Pinscher bitch, ovariohysterectomised eight years previously.

Abstract The presence of M. pachydermatis was investigated in 192 skin and ear samples originated from 50 dogs with atopic dermatitis. Correlation of M. pachydermatis isolation with age, sex, breed, living condition, onset of symptoms of atopic dermatitis (AD), intradermal test (IDT) results and sampling site was analyzed. M. pachydermatis was isolated from 49 samples (29 from the ears and 20 from skin). Greater number of isolates was noted in purebred dogs (29.8%) compared to the cross breeds (8.1%) (p<0.05). No statistically significant difference was found in the number of isolates between ear and skin samples (29.6% and 21.3%, respectively). Yeast isolation was more frequently noted from ear than from skin samples in dogs over 3 years of age, and in dogs with autumn and winter onset of symptoms. Out of 29 isolates from the ears, 28 (98.5%) originated from purebred dog samples (p<0.05). There was no statistically significant difference noted considering the otitis presence. However, this yeast was more commonly isolated from purebred dogs in a group without otitis (p<0.05). More frequent isolation was noted from leg and paw samples than from groin and hip samples in male dogs; while in dogs over 3 years of age, more frequent isolation was noted from ear samples than from groin and hip samples (p=0.05). In dogs with positive IDT to house dust and house dust mite, M. pachydermatis was more present in leg and paw than in groin and hip samples (p<0.05). Key words: dogs, atopic dermatitis, Malassezia pachydermatis

Equine pastern dermatitis (EPD), also known as grease, scratches or mud fever, represents variety of skin inflammatory conditions of the pastern region, all classified under this term. Therefore, it is rather considered a specific syndrome than a single disease diagnosis, with three main forms described. EPD has multiple potential etiologies (parasite (mite) infestation, bacterial infection, dermatophytosis, photosensitization, vasculitis, vaccinia, pemphigus foliaceus and primary irritant contact dermatitis), which are usually preconditioned with poor environmental conditions, particularly increased humidity and unappropriated hygiene. EPD has never been formally investigated in any area of Bosnia and Herzegovina. This is a preliminary investigation of the presence of EPD in the population of randomly selected horses in BiH. The research iincluded 40 horses of both genders and varied breeds and ages. EPD was detected in 12.5% of the examined horses with dominant presence in Arabian horses. Keywords: horse, equine pastern dermatitis, mud fever