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Katarina Aleksic, Ivana Stojković-Simatović, A. Stanković, Ljiljana Veselinović, S. Marković

In this study, ZnO as a semiconductor and RuO2 as a metal-like conductor were combined in a composite and tested as a photoanode for water splitting, i.e. green hydrogen and oxygen production. ZnO/RuO2 composite in the mass ratio of 2:1 was prepared using microwave processing of a precipitate. To investigate the influence of oxygen vacancies as defects in the crystal structure on (photo)electrocatalytic efficiency for hydrogen and oxygen production, the composite was annealed at 300 and 600?C. The phase composition, morphology, and optical properties of as-prepared and annealed composites were analyzed in detail. The (photo)electrocatalytic activity of composites for HER and OER was examined in NaOH and H2SO4 using linear sweep voltammetry. The (photo)electrocatalytic properties of the ZnO/RuO2 composites were correlated with their physicochemical characteristics with emphasize on oxygen vacancies.

E. Hodžić, Milica Balaban, Sebila Rekanovic, H. Makic

Heavy metals ubiquitously found in soil and water, as a serious environmental problem, are disrupting plant mineral nutrition homeostasis, osmotic balance, and metabolism. Application of some biostimulants can alleviate the disruption. Melatonin as a signal molecule, and antioxidant plays an important role in plant growth and stress tolerance due to its ability to directly neutralize reactive oxygen and nitrogen species. The reduction or mitigation of heavy metals adverse effects in valerian plants grown in open field conditions using melatonin was investigated in this paper. HPLC-FLD technique was used to identify and quantify melatonin concentration in valerian root extracts. Also, physiological, and biochemical plant status under abiotic stress was examined, especially in 100 ?M melatonin pre-treated plants. Higher concentrations of endogenous melatonin were measured in roots of Cd and Zn treated plants. Melatonin application alleviated the negative effect of Cd, particularly evident in Cd-Melatonin treatment which restored or enhanced bioactive compound levels. Melatonin effectively mitigates Cd and Zn-induced stress in valerian by enhancing both non-enzymatic and enzymatic antioxidant systems and promoting the synthesis of protective compounds. These findings highlight melatonin potential as a sustainable biostimulant to support plant resilience and productivity in heavy metal-stressed environments.

Mirza Valjevac, M. Bezdrob, S. Hamidović, Nermin Rakita, E. Karahmet, T. Gavrić, Berina Imamović

Red clover is one of the most important perennial forage legumes in livestock feed production. It also plays a significant role in organic farming, as it enriches the soil with nitrogen through symbiotic fixation by nodule-forming bacteria, reducing the need for excessive use of mineral nitrogen. The application of foliar biofertilizers can have a significant impact on the productivity and nodulation of red clover. Since the productivity and quality of red clover largely depend on soil fertility, environmental conditions, variety selection, and the plant’s developmental stage at the time of mowing, the aim of this research was to determine the influence of red clover variety and foliar biofertilizer (BF0, BF1, BF2, BF4) on dry matter yield, crude protein content, and the abundance of nodule-forming bacteria. The research results showed that variety selection and the application of biofertilizer at higher concentrations had a significant effect on dry matter yield and crude protein content. However, the total number of nodules on red clover roots decreased with increasing biofertilizer concentration. The highest annual dry matter yield was achieved in the BF4 variant for the Una variety (12.71 t ha-1) and the Global variety (11.43 t ha-1). Similarly, the highest crude protein yield was recorded in the BF4 variant for the Una variety (1896.4 kg ha-1) and the Global variety (1678.0 kg ha-1).

T. Gavrić, M. Bezdrob

Sweet corn (Zea mays L. var. saccharata) is one of the popular varieties of corn in the human diet. Its quality is influenced by various properties such as grain moisture, colour, ear weight, ear length, texture, and sugar content, while its health benefits are attributed to its total phenolic content and antioxidant activity. The aim of this research is to determine the impact of N fertiliser application and sowing date on yield indicators, total phenol content and antioxidant capacity of sweet corn. The treatments of the experiment consisted of a combination of two factors, two different nitrogen regimes and two different sowing dates. The results show that the applied treatments significantly influenced the researched traits of sweet corn. Dehusked and husked ear mass, ear length, and yield were higher when sown earlier (385 g, 264 g, and 21 cm, respectively) than later sowing (364 g, 242 g, and 20 cm, respectively). Additionally, higher values of dehusked and husked ear mass, ear length, were recorded with a higher dose of fertiliser. Furthermore, total phenol contents ranged from 57.0 to 80 mg GAE 100 g-1 and depended on sowing date, fertilisation, and year of study.

The Balkan Peninsula is one of Europe?s major hotspots for plant biodiversity. Although the rich flora of the Balkans has been exten?sively studied from many points of view, genome size studies have received comparatively little attention. This paper contributes to the online available Genome Size Database of Balkan Flora (GeSDaBaF; http://www.pmf.unsa.ba/gesdabaf) and updates this database with new bibliographic data on the C-values for plants in the Balkan region. Additionally, 2C DNA values determined by flow cytometry are reported for 87 accessions, encompassing 82 taxa (70 species, 11 subspecies, and one hybrid taxon, 14 of which are endemic to the Balkans and two with amphi-adriatic distribution) across 63 genera and 31 families. The newly obtained genome size values include the first data for one genus, 25 species, eight subspecies, and one hybrid taxon. These new data represent 9.99% of the 821 taxa for which nuclear DNA content has been assessed in the Balkans to date, highlighting existing knowledge gaps for future research.

Georgiev Milošević, Lea Neškov, Sonja Nikić, Valentina Marinković, Marina Odalović, Dušanka M. Krajnović

Customer satisfaction reflects the quality of pharmaceutical services and depends on various factors, such as the quality of services, pharmacists' skills and customer' demographic characteristics. Positive experiences strengthen customer trust and loyalty. Research into user satisfaction is an important tool for improving services. The aim of the work was the analysis of user satisfaction with pharmacy services in Serbia over the last twelve years. Annual reports on user satisfaction in pharmacies from 2013 to 2024 were analyzed. Based on key indicators, a report was prepared comparing satisfaction over the years, taking into account various factors and service quality. With the exception of 2020, public, private and health center pharmacies participated every year. The highest response rate was recorded in 2015. More than 60% of users visit a pharmacy more than five times a year, usually waiting less than five minutes. The highest level of satisfaction with the availability of medicines was recorded in 2021, while overall satisfaction with services peaked in 2022. User satisfaction has remained stable, indicating good availability and quality of pharmaceutical services. The results may contribute to further improvement in practice.

I. Lutvikadić, A. Šunje-Rizvan, S. Kučlar Muftić, A. Alić, J. Šupić, A. Maksimović

Background: Coeliotomy in chelonian species is performed to gain access to the abdominal cavity and requires general anesthesia. In reptile medicine, alfaxalone and propofol are often preferred, although the usage of these drugs is limited by cost and/or vascular access. Furthermore, according to a recent study, propofol produces significant oxidative stress in red-eared sliders (Trachemys scripta elegans), and in such cases, a ketamine and medetomidine combination may provide a better choice. Case description: Three owned red-eared sliders were separately presented with a history of recent lethargy, inappetence, dyspnea, and restlessness. Findings/treatment and outcome: In these three cases of chronic dystocia, a ketamine and medetomidine combination was used to achieve anesthesia induction for surgical treatment. Additionally, intrathecal administration of lidocaine was performed to achieve a potential analgesic effect in deeper intracoelomic reproductive organs. Preoperative changes in blood parameters were observed in one animal, suggesting abnormal kidney function. The same animal showed signs of resedation postoperatively, prolonged recovery, and had a fatal outcome 48 h after the surgery with signs of kidney damage in histopathological evaluation. In the recovery period, none of the surviving animals (n=2) showed neurological or respiratory complications that could be associated with subcarapacial sinus anesthesia administration or intrathecal local anesthesia. Conclusion: According to our observations, intrathecal lidocaine administration provided sufficient analgesia during prefemoral coeliotomy and reproductive organ manipulation. These modes of anesthesia in chelonians require further investigation.

Aleksandar Jovanović, M. Drobac, Ivana Tadić, Bojana B Vidović, D. Pavlović, R. Veličković-Radovanović, Marina Odalović, Ana Kundalić et al.

From patients' perspective, the use of antibiotics to treat urinary tract infections (UTIs) in Serbia is unexplored, and therefore the aim of this study is to examine antibiotic use among these patients. An online cross-sectional study using snowball sampling was conducted during the winter of 2020/21 using a validated Google Docs questionnaire. The study included 236 female patients with a mean age of 34.9 ± 14.2 years. Most of the patients perceived UTI symptoms as severe (62.3%) and disruptive for their daily routines (51.3%). The majority of the patients (77.1%) used antibiotics by doctors' prescriptions. Other patients used antibiotics on their own and their selection. Self-treatment with antibiotics was associated with perceived symptom severity (p=0.006) and residence (p=0.017). In total, 17 different antibiotics were reported as being used for UTI treatment and the most frequent were fluoroquinolones (30.3%), cephalosporins (21.2%), and sulfonamides (15.7%). The highest consistency with national and European guidelines in doctors' decisions on antibiotic therapy was observed for treating UTIs in pregnant women (80.0%). These findings emphasize the need for education of healthcare professionals and clinical practice improvement in making rational antibiotic prescribing decisions.

Georgiev Milošević, Lea Neškov, Sonja Nikić, Dragiša Obradović, Valentina Marinković, Marina Odalović, Dušanka M. Krajnović

The college years are critical to students' health as they face various challenges, including social influences and unhealthy lifestyles. Limited access to health services in residence halls increases the risk of unhealthy behaviours. The aim of this study was to investigate students' attitudes towards public health services for primary prevention in dormitories and to make suggestions for improvement. A cross-sectional study was conducted using a questionnaire among students living in halls of residence. The survey explored students' views on healthcare and the availability of preventive services for students living away from home. A total of 996 students participated (response rate 99.1%). Most were unemployed (83.9%). The most frequent preventive visits were to dentists (34.4% twice a year, 24.7% once a year), while 37.7% never visited a gynaecologist/urologist. The majority (68.6%) stated that a pharmacy was necessary in their dormitories. Basic health services include general practitioners (82.3%) and psychologists (21.4%). Attitudes towards health centres varied significantly by place of residence (p<0.001). Primary prevention should be improved by health centres with student-friendly opening hours and additional activities that promote access to healthcare and awareness of prevention.

Danijel Bijedic, E. Becirovic, Jasminka Petrović, Humera Jahic, Alma Trnacevic, Azra Zigic

Background: Depressive disorder is characterized by a persistent low mood (sadness, irritability, or emptiness) or a loss of pleasure, accompanied by other cognitive, behavioral, or neurovegetative symptoms that significantly impair a person's ability to function. Anxiety and fear-related disorders are marked by excessive anxiety and fear, which lead to behavioral disturbances and cause significant distress or impair personal, family, social, educational, occupational, or other key areas of functioning. Objective: The aim of this study was to analyze whether there are differences in the development of depressive symptoms between COVID-19 patients and patients with symptoms of the flu or common cold. Methods: This is a prospective study that included sixty participants: thirty with COVID-19 and thirty without COVID-19. Data were collected from the ambulatory observational records of participants, documenting their symptoms. We analyzed the relationship between variables such as age, sex, education, and the level of potential depression. The participants were divided into two groups: one consisting of individuals with COVID-19 and the other of individuals without COVID-19. All participants were male and female, aged between 40 and 65 years, and had no previous history of depressive disorder. The first group consisted of patients with COVID-19 who were treated at home but were examined at our outpatient clinic and subsequently sent home. Results: Statistical data processing was performed using the Excel program and the R statistical data processing program. Percentages are calculated in relation to 30 respondents in each group.There was no statistically significant correlation between the level of education in Group 1 and the development of depression(Spearman ro=0.007, P=0.972). In Group 1 (Covid ) there is a statistically significant correlation between the age of the subjects and the development of depression (Spearman ro=0.44, P=0.015). A positive value of the Spearman correlation coefficient means that the score increases with the age of the respondent. Three questions with the highest score in group 1 were: Question 2=71, Questions 15 and 16=66, Question 14=65; three questions with the highest score in group 2 were: Question 2=74, Question 1 =50, Question 18=46. Conclusion: COVID-19 can contribute to the development of depression. In our study, 16.7% of patients with COVID-19 showed signs of depression, with 10% experiencing mild depression and 6.7% experiencing moderate depression. Patients in the first group, particularly those of older age, were more likely to develop mild or moderate depression associated with COVID-19. Additionally, no statistically significant correlation was found between the level of education in Group 1 and the development of depression.

According to the WHO, health is not just the absence of disease, but a state of complete physical, social and mental well-being, which is why the main goals of health are aimed at improving physical, mental and spiritual health. Health is one of the fundamental rights of every human being, a precondition for prosperity and quality of life indicator for measuring progress and the basis of steady economic growth. Today, many professions are committed to modernizing the culture of relations with the public, i.e. citizens. This is also essential in healthcare, with the aim of placing the patient’s well-being and rights at the center of attention, i.e. a culture of patient-centeredness. In order to ensure that the rights of every patient are respected, a more efficient system of protecting patients’ rights is needed at all levels, and above all in hospitals, and, patient representatives should be appointed in all hospitals. The health status of our population depends on a rapid changes, such as number of demographic, social, cultural, ethnical, and other characteristics which are for several decades in a very intensive changes, The Jakarta Declaration identified five priorities: a) Promoting social responsibility for the state of health; b) Increasing investments for development of health; c) Development of partnerships for work on health promotion; d) Increasing the capacity of society and training the individual; e) Provision of infrastructure for health promotion. Analyzes of the effectiveness of preventive activities represent a systematic assessment of the impact of public health policies, programs and practices on health outcomes. Based on them, it is possible to create basic recommendations related to public health programs, guidelines for prevention and control, and making decisions about the allocation of available funds.

Izet Masic, Lars Lindsköldm, Petter Hurlen, Paris Gallos, John Mantas, Catherine Chronaki, Arriel Benis, George I. Mihalas et al.

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