Background: Bosnia and Herzegovina (B&H) is one of the countries of Southeast Europe with the lack of data about chronic autoimmune thyroid diseases (CAITD) epidemiology. Objective: This research aimed to assess incidence of CAITD in the Tuzla Canton of B&H during a 6-year period (2015–2020). Methods: We retrospectively evaluated 82,000 hospital records of inpatients and outpatients with possible thyroid symptoms residing in Tuzla Canton of B&H (total of 445,028 inhabitants). The study included patients with laboratory and clinical proof of autoimmune thyroid diseases (AITD). Incidence rates were calculated with age standardisation using European standard population. Trends in incidence were evaluated as moving three-year averages. Results: During the observed period, 1875 patients satisfied the diagnostic criteria for CAITD with male to female ratio of 1: 8.01. Median age of all cases was 46 years (interquartile range: 31 to 61) and women and men were on average the same age at the time of diagnosis. The overall standardized incidence was found to be 71.25 per 105 (%95 CI=63.36–79.15). The overall standardized incidence in men was 16.25 per 105 and 123.74 per 105 in women. In the end of the observed period, AITD prevalence was 427.52 per 105 (% 95 CI=408.17-446.87). Conclusion: There was a slight decline of incidence in our region during the observed period. This decrease might be the result of combination of various factors, mainly the Corona epidemics outbreak and emigration. On the basis of the lower incidence rate in the Tuzla Canton, one can assume that iodine prophylaxis carried out in order to eradicate goitre had satisfied expectations because there had not been any enormous increase in patients with AITD.

Negativni efekti izvođenja radova u sektoru šumarstva sa aspekta zdravlja i sigurnosti radnika posebno su izraženi u okviru tehnološkog procesa iskorištavanja šuma. Sječa stabala i izrada šumskih drvnih sortimenata u Bosni i Hercegovinin obavlja se motornim pilama, a privlačenje drveta skiderima u najvećem broju slučajeva. Cilj istraživanja prikazanog u ovom radu je utvrđivanje opterećenja radnika u fazi sječe i izrade šumskih drvnih sortimenata iz razloga djelimične mehanizacije radova, tj. upotebe motor-manuelnog sredstva rada. Težina rada može se procijeniti na osnovu pulsa radnika, poređenjem pulsa mjerenog tokom odmora i rada, što je zbog praktičnosti veoma pogodan metod za istraživanja u oblasti šumarstva. Mjerenje pulsa sjekača obavljeno je uz upotrebu Garmin Forerunner 35 pametnog sata za trčanje (Garmin Ltd., United States) sa kontinuiranim mjerenjem i čuvanjem podataka. Rad sjekača sniman je akcionom kamerom tokom cijelog radnog dana. Istraživanje je provedeno u mješovitim raznodobnim šumama bukve i jele sa smrčom na području kojim gazduju KJP „Sarajevo šume“  i JP „Šumsko - privredno društvo Zeničko - dobojskog kantona“  d.o.o. Zavidovići. Prosječan puls tokom rada uključujući produktivno vrijeme i prekide rada iznosi 117 o/min (otkucaja u minuti) za radnika A, odnosno 113 o/min za radnika B. Rezultati Kruskal-Wallis testa su pokazali postojanje statistički značajnih razlika u vrijednostima prosječnog pulsa u zavisnosti od elementa studija rada (radne operacije i prekidi rada). Najmanje vrijednosti pulsa evidentirane su tokom pripremno-završnog vremena za oba radnika, a najveće vrijednosti tokom hoda do radilišta za radnika A, odnosno rješavanja ustave za radnika B. Prosječni puls tokom rada (%HRR) za radnika A iznosi 47.15%, odnosno 50.00% za radnika B. Rezultati istraživanja su pokazali da opterećenje sjekača prelazi dozvoljenu vrijednost od 40% što odgovara teškom radu i može imati negativni uticaj na zdravlje radnika. Uzimajući u obzir da su utvrđene nešto veće vrijednosti prosječnog pulsa tokom rada (%HRR) u odnosu na rezultate drugih istraživanja neophodno je razmotriti mjere za njegovo smanjene a koje bi podrazumijevale obuku radnika, promjene u organizaciji rada i kontinuiranu procjenu radne sposobnosti radnika.

The paper investigates the phenomenon of soil erosion on skid roads/trails. The aim was to discover how and to what extent different longitudinal slopes can influence the occurrence of erosion processes. For this purpose, a total of nine experimental plots were set up at three different sites. The length of the plots was 110 m, and at all localities, the research was carried out on different longitudinal slopes (up to 10%, 10 - 20% and over 20%) over the observed period of approximately two and a half years. The research focused on determining the volume of material removed and the dynamics of the development of erosion processes observed in phases (shifts) between recordings. Forests are the most effective natural factor in protecting land from erosion, and if they are managed on the principles of sustainable management, the so-called "normal" erosion usually occurs. However, as it is necessary to build a relatively dense network of skid roads/trails for different phases of management, it makes them susceptible to more intensive erosion processes. If we take into account that they were built with very low criteria (technical elements) and without the existence of facilities that would ensure the drainage of surface and rainwater, and can be built with a large longitudinal slope, it creates optimal preconditions for the development of intensive erosion process. The conducted research has obtained results that clearly show that erosion processes occur frequently, of varying intensity depending on the factors that prevail for a given locality. The value of the volume of the removed material ranges from 1.278 m3, ie 0.0116 m3/m to 5.313 m3, or 0.0483 m3/m. The obtained minimum value of the volume of the removed material can be related to the strong water permeability of the parent substrate (limestone), which affects the reduction of surface runoff, as well as shallow soils that are formed on it. When it comes to the maximum value of the volume of removed material, which is 5.333 m3 or 0.0483 m3/m, it can be related to the continuous occurrence of surface runoff that causes surface sapping and removal of material from the skid roads/trails. The performed statistical analyzes (through a linear model) indicate different correlations of investigated influencing factors (volume of transported material and length of skid roads/trails on different longitudinal slopes). The obtained correlation values range from r = 0,29 (weak correlation) to r = 0,79 (very strong correlation).

Psoriasis is an autoimmune and inflammatory skin disease. Psoriatic patients express higher levels of plasma homocysteine (Hcy) concentration and pro-inflammatory mediators than healthy people; this is frequently associated with vitamin D deficiency. The aim of this clinical study was to investigate the effects of high doses of vitamin D supplementation on the parameters of Hcy metabolism and cytokines in sera of psoriatic patients. This prospective study was conducted on 40 psoriatic patients who had the vitamin D deficiency. All patients received vitamin D 5000 IU/day for three months. Clinical and biochemical measurements were taken at baseline and at follow up (3 months). The results showed that the severity of clinical features, measured by the psoriasis area severity index (PASI) score, were considerably improved in patients after vitamin D supplementation. After vitamin D supplementation, most of the patients (n = 25 or 62.5%) had mild clinical form (p < 0.001). After twelve weeks of intervention period, there were significant increases in vitamin D and B12 serum levels in comparison to the levels that had been measured at the beginning of the study (56.77 ± 14.66 nmol/L and 301.08 ± 95.02 pg/mL vs. 103.85 ± 32.20 nmol/L and 362.81 ± 118.56 pg/mL, respectively; p < 0.001). Moreover, serum levels of Hcy and folate were significantly lower at the end of the study in comparison with the initial levels (12.45 ± 1.92 µmol/L and 8.01 ± 3.88 mg/mL vs. 10.38 ± 1.66 µmol/L and 6.27 ± 2.60 mg/mL, respectively). High doses of vitamin D supplementation led to a significant decrease in pro-inflammatory cytokines (IFN-ɤ, TNF-α, IL-1β, IL-6, IL-8, and IL-17) and high-sensitivity C-reactive protein (hsCRP), whereas the production of anti-inflammatory cytokines (IL-10, IL-5) was up-regulated. In conclusion, supplementation with high doses of vitamin D could be one of the possible preventive and therapeutic measures to reduce systemic inflammation in psoriatic patients.

BACKGROUND patients with different types of dementia may experience changes in nutritional status, which are manifested by specific eating habits. The aim of this study was to determine the nutritional status and eating habits of patients at the time of confirmed diagnosis of primary progressive dementia. SUBJECTS AND METHODS The study included 40 outpatients (63% women) diagnosed with either form of dementia. The mean age at diagnosis was 77±6 years and the mean time between the onset of first symptoms of the disease and diagnosis was 3-36 months. Nutritional assessment was determined at the time of confirmed diagnoses and included dietary habits (non-quantitative modified food frequency questionnaire (FFQ)), anthropometric (body weight and height and body mass index-BMI) and biochemical parameters (serum concentrations of vitamin B12, folic acid and 25-hydroxy vitamin D). Dietary habits were collected over a 12-month period with the help of a spouse or close family member. RESULTS The results showed that none of the outpatients were malnourished, the largest number of outpatients (43%) were in the normal body mass category followed by 33% in the overweight category. The results of this study confirmed previous findings of higher preference for sweet foods observed in 53% of patients with dementia. Low status of vitamin B12 was observed in 57% of outpatients, folic acid in 24% and 25 (OH) D in 75% of outpatients. Lower frequency of consumption of dark green leafy vegetables and lower consumption of poultry meat, fish and eggs could have an impact on nutrient deficiency. CONCLUSIONS The poor nutritional status of outpatients with primary progressive dementia is associated with unhealthy dietary habits that may lead to micronutrient deficiencies. Dietary monitoring and intervention should be initiated immediately after the diagnosis of primary progressive dementia with the goal of reducing nutritional deficiencies and preventing further and more severe impairment of cognitive function.

Abstract Interspecific hybridization in the Cyprinidae family has been recorded worldwide, with Abramis brama (bream) and Rutilus rutilus (roach) as one of the often-reported hybridizing pairs. The only account of such an event in Bosnia and Herzegovina has been in Modrac Reservoir. Using morphological and molecular markers, the presence of hybrids was surveyed, the hybridization direction was determined and the hybrid group structure in this ecosystem was evaluated. Our findings confirmed unhindered natural hybridization between roach and bream in Modrac Reservoir. Over 50% of the hybrid specimens were classified as F2 hybrids by the NewHybrids software, while the rest were categorized as pure parental form, making it the first such finding in Europe. The analysis of mitochondrial cytochrome b showed that 90% of hybrid individuals were of bream maternal origin. The hybrid group expressed higher mean values of observed heterozygosity and gene diversity than both parental species. Signs of introgressive hybridization between parental species were detected. The hybrid zone of Modrac Reservoir appears to follow the intermediate or “flat” hybrid model based on the balanced distribution of parental and hybrid genotypes. Further investigation is needed to elucidate the factors that enable the survival and mating success of post-F1 individuals.

Background: Non-alcoholic fatty liver disease (NAFLD) is an increasingly common cause of chronic liver disease and is becoming a major public health problem. NAFLD has been recognized as a hepatic manifestation of metabolic syndrome, associated with systemic diseases such as cardiovascular disease (CVD) and chronic kidney disease (CKD). Objective: The aim of this study was to examine the role of serum LFT parameters and renal function parameters as predictors of unmanifested liver disease. Methods: In this study, the presence of possible liver disease detected by biochemical parameters and confirmed by Transient Liver Elastography (TE) in a group of patients with different stages of chronic kidney disease (CKD) was investigated. Patients with various stages of CKD were divided into five subgroups regarding aetiology: nephroangiosclerosis, diabetic nephropathy, glomerulonephritis and pyelonephritis, autoimmune kidney disease, and polycystic and another morphological kidney disease. Liver stiffness was used to quantify liver fibrosis while Controlled attenuation parameter (CAP) was used to quantify liver steatosis. Functional liver tests and biochemical parameters of kidney function were measured in all patients. Results: Statistical analysis used in this study was a decision tree as a predictive model to map observed variables resulting in the conclusion about outcomes. The application of existing laboratory parameters, in combination with other parameters in presence of the defined etiological factors of kidneys diseases, indicate development of hepatic diseases. Higher values of phosphorus and low values of ferritin in patients with autoimmune kidney disease, and polycystic and another morphological kidney disease, expresses steatosis of the hepatic parenchyma. Conclusion: In contrary, low values of phosphorus and higher values of ferritin in patients with nephroangiosclerosis, diabetic nephropathy, glomerulonephritis and pyelonephritis, are in a favour steatosis of the hepatic parenchyma. Serum values of phosphorus and ferritin are valuable predictors of the liver disease in patients with end-stage kidney diseases of different aetiology.

Apocrine carcinoma of the breast is a rare malignancy. According to 2019 WHO classification, apocrine cellular features and a characteristic steroid receptor profile (Estrogen receptor (ER)-negative and androgen receptor (AR)-positive) define apocrine carcinoma. Her-2/neu protein expression is reported in ∼30-50% of apocrine carcinomas, while NGS analysis showed frequent PIK3CA/PTEN/AKT and TP53 mutations Followed by deregulation in the mitogen-activated protein kinase pathway components (mutations of KRAS, NRAS, BRAF). A recent miRNA study indicates various miRNAs (downregulated hsa-miR-145-5p and upregulated 14 miRNAs such as hsa-miR-182-5p, hsa-miR-3135b, and hsa-miR-4417) may target the commonly altered pathways in apocrine carcinomas such as ERBB2/HER2 and mitogen-activated protein kinase signaling pathway. Although AR expression is a hallmark of apocrine carcinoma, little is known regarding the efficacy/resistance to antiandrogens. Success of bicalutamide, a non-steroidal anti-androgen, was reported in a case of Her2-negative apocrine carcinoma. Two recent studies, however, described presence of anti-androgen resistance biomarkers (a splice variant ARv7 and AR/NCOA2 co-amplification) in a subset of AR+ apocrine carcinomas, cautioning the use of anti-androgens in AR+ triple-negative breast carcinomas. Apocrine carcinomas rarely show biomarkers predictive of response to immune checkpoint inhibitors (PD-L1 expression, MSI-H status, and TMB-high). Therefore, a comprehensive cancer profiling of apocrine carcinomas is necessary to identify potential therapeutic targets for a truly individualized treatment approach.

Background: Autism spectrum disorder (ASD) is a developmental disability, which is a biologically based neurodevelopmental disorder that affects a child’s social interaction and communication skills. Core deficits are identified in two domains: social communication/interaction and restrictive, repetitive patterns of behavior. Children and youth with ASD have service needs in behavioral, educational, health, leisure, family support, and other areas. Autism is a set of heterogeneous neurodevelopmental conditions, characterized by inability to acquire social skills, repetitive behaviors and failure of speech and nonverbal communication development. Objective: To examine frequency, correlation and predictivity of communication and language skills of autistic spectrum disorders (ASD) children and their parents’ emotions. Methods: The sample consisted of 80 participants who are parents of children with ASD. The study was performed using a questionnaire made out for this research. Three groups of variables were analyzed: parental emotions variables; child’s communication variables, child’s language variables. Statistical analysis was performed by SPSS, and included basic statistical parameters and multiple regression analysis. Results: The most common ways of communicate for children with ASD are: Allowing to cuddle; Recognizes familiar faces; and Makes it known when he needs help or wants an item. The most common language forms of children ASD are: Following simple instructions; Understanding connected words/phrases; Pronouncing single words; and Looking at when called by name. Parents of children with ASD expressed the greatest agreement with the following statements: “My child is more demanding than most other children”; “It seems to me that my child is very sensitive and easily upset”; “My child can’t do much of what he’s expected to know to do”, and “I often lack energy.” Conclusion: Parents of children with ASD proved that their children have significant delays in communication and language. Developed communication and language skills of the child with ASD are valid predictors of parents’ emotions/attitudes. Speech and language therapy work focused on developing the ASD child’s communication and language skills can be expected to improve parents’ emotions/attitudes.