Introduction Autistic spectrum disorders belong to the group of neurodevelopmental disorders, where the manifestations of the disorder differ depending on the severity of the autistic condition. These disorders are often associated with intellectual impairments and structural language disorders. In his patients, Asperger described cases with above-average intelligence as well as cases with low language and intellectual abilities. What they had in common was that they had significant disorders in social and affective communication. The diagnosis of autism spectrum disorders is usually made in childhood, but there are also cases where the diagnosis is made at an older age due to the later manifestation of symptoms. Objectives To present a case report of 18 years old girl who was diagnosed with moderate intellectual disability in childhood, while later established with symptoms of Asperger’s syndrome and mild intellectual disability. Methods Psychiatric interview, medical history, psychological testing Results An 18-year-old girl, accompanied by her mother, presented herself to psychiatrist for the first time due to affective disorders that manifested in the form of increased nervousness and outbursts of anger. Early psychomotor development was slow, she started walking at the age of 1.5 years and spoke at the age of two. From early childhood, stereotypies in movements and hypervigilant attention were observed, along with very poor social interactions. Due to difficulties at school, she was categorized at the age of 12 as having moderate intellectual insufficiency and IQ of 35. She underwent long-term speech therapy treatment and finished elementary and high school with the help of teaching assistant. Somewhere in high school, the patient’s pronounced talent for drawing was noticed. Observation during the psychiatric examination reveals emotional immaturity, stereotypes and rigidity in social interactions. The patient is referred again for psychological testing, results show IQ of 88 with a lag in emotional development and development of basic social skills. The patient is referred to another psychiatrist for an additional opinion, who agrees with diagnose of Asperger’s syndrome. Low dose of lamotrigine was included in therapy, after which affective state stabilized. The patient is referred again for categorization. Conclusions This case report that a diagnosis established in childhood period does not have to be definitive and that revision of diagnosis is necessary over time and as necessary, initially due to increase of patient’s IQ, as well as due to the later presentation of symptoms characteristic of certain disorder. Disclosure of Interest None Declared

Background: The incorporation of polymers into drug delivery vehicles has been shown to be an effective strategy to prolong the residence time of active ingredients in the precorneal tear film and to increase ocular bioavailability. Objectives: The aim of this study was to develop novel, viscous eye drops containing olopatadine (OCH) as the active ingredient, polysaccharides hydroxypropyl guar gum (HPG), and sodium hyaluronate (SH), individually, and in combination as functional polymers. Methods: Viscous eye drops containing 0.1% OCH in combination with HPG (0.25%) and SH (0.4%), were prepared and evaluated for their physicochemical properties, rheological behavior, mucoadhesion, and preliminary stability. A novel rheological method was used to evaluate the resistance of the eye drops under simulated blinking conditions. In vivo efficacy was evaluated using an ocular itch test in mice to compare the formulations with a commercial product. Results: The formulations remained stable and transparent, with physicochemical parameters within acceptable ranges. Rheological studies confirmed pseudoplastic flow, with the HPG-SH combination exhibiting enhanced viscosity and shear-thinning properties for prolonged retention in the eye. Mucoadhesion was highest in SH-HPG formulations. During simulated blinking cycles, eye drops containing a combination of SH and HPG polymers fully regained their initial viscosity during the resting periods. Preliminary stability studies indicate that the formulated eye drops exhibit satisfactory physicochemical stability under various storage conditions. In vivo, OCH-SH and OCH-HPG-SH drops provided prolonged antipruritic and analgesic effects compared to the reference product. Conclusions: Polysaccharide-based innovative formulations improve OCH retention, enhancing therapeutic efficacy and patient compliance in the treatment of allergic conjunctivitis.

Six novel green extraction techniques were evaluated and optimized to extract pyrethrin from dried Dalmatian pyrethrum (Tanacetum cinerariifolium (Trevir./Sch.Bip.). This approach offers a promising natural alternative to conventional chemotherapeutics. Four methods are presented for the first time in this study: microwave-assisted extraction (MAE), high-voltage electric discharge (HVED) extraction, subcritical water extraction (SWE), and deep eutectic solvent (DES) extraction, together with supercritical CO2 extraction (SC-CO2) and ultrasound-assisted extraction (UAE), for pyrethrin extraction from Dalmatian pyrethrum. The study revealed that supercritical CO2 extraction was the most effective method for extracting all six pyrethrins, yielding the highest total amount of 124.37 ng/mg. This approach offers a “natural” insecticide produced with a clean, environmentally friendly technology that can contribute to the development of sustainable and effective insecticide strategies that are in line with environmental safety and organic production standards. In addition, this research highlights the potential application of pyrethrins as antiparasitic agents, emphasizing their role in environmentally friendly and ecological practices.

Introduction Faculty of Medicine is often recognized as one of the most laborious. Student workload, constant exposure to stressful situations, fear of failure, pressure from parents, exposure to death and human suffering are some of the many factors associated with increasing levels of anxiety and depression among medical students. Objectives The aim of this study was to estimate the severity of anxiety and depression disorders among medical students. Methods In this cross-sectional study, self-report anonymous online survey was distributed to the students of all six years of the Faculty of Medicine, University of Sarajevo. In this survey, which consisted of 33 questions, we gathered general information and the Beck’s Anxiety and Depression Inventory were used to assess the severity of anxiety and depression, whereby respondents received points by answering each question, and after scoring, they were categorized in different groups based on the severity of anxiety and depression. Results 129 students completed the survey. Considering the Beck’s Anxiety Inventory, 56% scored for the “Low anxiety” group, 33% scored for the “Moderate Anxiety” group, 11% scored for the “Potentially concerning levels of anxiety”. Considering the Beck’s Depression Inventory, 43% of the students scored for the group “These ups and downs are considered normal”, 21% scored for “Mild mood disturbances”, 9% for the “Borderline clinical depression”, 19% for “Moderate depression”, 6% for “Severe depression”, 2% for “Extreme depression”. Conclusions Psychiatric morbidity found needs to be identified and treated at the earliest, because it can lead to suicidal ideation. Medical students should be encouraged to seek help and adequate facilities should be available to all of them. Disclosure of Interest None Declared

U ovom radu predstavljeni su podaci o spahijama na području današnje općine Hadžići kroz popise koji obuhvaćaju period od 1828. do 1831. godine, a čuvaju se u Arhivu Orijentalnog instituta u Sarajevu u okviru zbirke sidžila pod brojem S 68. Rad se sastoji od tri cjeline. U prvoj uvodnoj cjelini predstavljeni su podaci o sadržaju sveska S 68, dok je druga cjelina rada poslužila za osvrt na izvore i literaturu vezane za hadžićki kraj u prvoj polovini 19. stoljeća kao perioda u kojem su nastali tretirani popisi. Treća i najopširnija cjelina rada tematizira podatke vezane za spahije hadžićkog kraja u svesku S 68 uz poseban osvrt na karaktere popisa te na određene nedoumice vezane za administrativno teritorijalnu pripadnost ovog kraja u prvoj polovini 19. stoljeća.

Background Angiotensin II (Ang II) plays a critical role in the progression of kidney disease. In addition to its direct signaling events, Ang II transactivates epidermal growth factor receptor (EGFR) and causes renal injury. CD148 is a transmembrane protein tyrosine phosphatase that dephosphorylates EGFR and strongly inhibits its activity. In this study, we have asked if CD148 agonistic antibody 18E1 mAb attenuates renal injury induced by chronic Ang II infusion to explore its therapeutic application. Methods Hypertensive nephropathy was induced in mice subjected to unilateral nephrectomy (UNx) by infusing Ang II (1.4 mg/kg per day) for 6 weeks using an osmotic minipump. The 18E1 mAb or isotype control IgG were intraperitoneally injected (15 mg/kg, three times per week) to the UNx + Ang II mice for 6 weeks, and their renal phenotype was investigated. Results Chronic Ang II infusion induced evident hypertension and renal injury that is indicated by elevation of plasma creatinine, urinary albumin excretion, renal hypertrophy, podocyte injury, macrophage infiltration, and the expression of alpha smooth muscle actin and collagen deposition. As compared with isotype control antibody, 18E1 mAb significantly reduced these renal changes, while it showed no effects on blood pressure. Furthermore, phospho-EGFR immunohistochemistry and immunoblotting demonstrated renal EGFR is activated in the mice that were subjected to UNx and Ang II infusion and 18E1 mAb significantly reduces EGFR phosphorylation in these kidneys as compared with isotype control treatment. Conclusion Agonistic CD148 antibody attenuates UNx + Ang II–induced renal injury, in part by reducing EGFR activity. Supplementary information The online version contains supplementary material available at 10.1186/s12882-025-04070-x.

This study explores pyrometallurgical and hydrometallurgical methods for decarbonizing and recovering valuable metals from bauxite residue, with direct hydrogen reduction in a rotary kiln without smelting and dissolution of solid residues under high pressure in an autoclave. The goal is to offer decarbonizing techniques for the removal of iron from bauxite residue, a by-product of the Bayer process, which cannot be disposed of in an environmentally sustainable manner. In contrast to traditional carbon-based reductive melting, which generated significant CO₂ emissions, hydrogen is now being investigated as a cleaner alternative. Through hydrogen reduction in rotary kiln, approximately 99.9% of iron is recovered as iron, which can be separated using magnetic separation from the solid residue containing other valuable metals. We concluded that hydrogen can reduce iron oxide from bauxite residues to metallic iron in contrast to very stable oxides such as titanium oxide, silica and aluminum oxide. Leaching of titanium, iron and aluminum with sulfuric acid has high efficiency under high pressure in an autoclave.

The chemical reactive molecule ROS (Reactive Oxygen Species) is a product of normal cellular metabolism. ROS plays a pivotal role in a wide range of biological processes, including aging, cancer and neurodegenerative diseases. Recent studies have shown that ROS can also affect the ribosomes – molecular machines responsible for protein synthesis.  ROS leads to errors in protein synthesis and the production of misfolded proteins, causing damage to ribosomes. However, it has also been suggested that ROS is implicated in the regulation of the ribosome activity under certain conditions. The aim of this paper is to review current knowledge regarding the effects of ROS on ribosomes, with a focus on the mechanisms by which ROS can cause damage to ribosomes and the potential role of ROS in regulating ribosome activity.

Objective. The study assesses how well patients follow secondary prevention measures after completing an outpatient cardiac rehabilitation (CR) program. Materials and Methods. This research involves 63 patients who completed an outpatient CR program between 2018 and 2020 at the Istrian Health Center in Pula, Croatia. Socio-demographic characteristics, levels of physical activity, adherence to a Mediterranean diet, and compliance with medical recommendations were assessed in a survey to gather data. Results. Out of the 63 patients surveyed, 51 responded (34 males, 17 females, average age 68.25 years). After completing a cardiac rehabilitation program, 78.4% maintained appropriate levels of physical activity but showed low adherence to a Mediterranean diet, with an average score of only 3.94 out of 9. Only a small percentage (2%) effectively followed medical recommendations. Conclusion. Despite the fact that patients maintain physical activity, there are still challenges regarding adherence to nutritional recommendations and medical advice. Long-term adherence can be improved by utilizing technology, involving family members, and offering education. Future research should focus on identifying obstacles to adherence and developing approaches to achieve lasting behavioral change.

Quantum Key Distribution (QKD), a secret key agreement primitive, makes possible long-awaited real-world Information-Theoretical Security (ITS). In the last twenty years, the development of QKD-based networks that deliver ITS keys to distant parties has been a focus of the academic and industry sectors. Several key-delivery specifications have been developed for the practical delivery of keys to end applications. In this paper, we discuss key-delivery specifications with a focus on security and authentication.

The development of telecommunications networks sets greater appetites to ensure secure communication flows. One of the approaches to providing information-theoretical levels of security is the application of quantum cryptography based on the quantum laws of nature. However, quantum networks differ significantly from existing networks in terms of their organization and availability of resources. This technology’s convergence largely depends on how it is integrated into existing networks and on the economic return of investment. Towards analyzing the latter, we consider the business model for accessing QKD network resources through a mechanism of pricing a QKD link upon a user’s request, with continuous fairness monitoring of the network utilization.

Future connected cooperative automated mobility can benefit from high-data-rate wireless communication links between vehicles to exchange LIDAR and/or RADAR data. The millimeter wave (mmWave) frequency range offers large bandwidth for rapid sensor data exchange but suffers from higher signal attenuation compared to the centimeter wave (cmWave) band. In this paper we obtain cmWave and mmWave multipath component (MPC) parameters by combining a single omnidirectional antenna that is used as virtual array with the CLEAN algorithm. The cmWave MPC information enables low-overhead mmWave beamforming by means of an antenna array. Empirical multi-band measurement data ($3.2 \text{GHz}, 34.3 \text{GHz}$, and 62.35 GHz) shows that considering the 5 strongest MPCs, a signal-to-noise ratio of up to 25 dB for a V2I scenario can be achieved.