Introduction: Cognitive impairment is the most common neurological disorder associated with brain tumors, which leads to linguistic communication damage. Anxiety and depression are among the most prevalent psychiatric comorbidities in this population. Objectives: To determine the level of cognitive functioning, anxiety, and depression in patients with intracranial tumors with and without aphasia. Subjects and methods: A prospective study was conducted on 91 patients with verified brain tumors which were hospitalized at the Clinic for Neurosurgery for one year period. The patients were assessed with Mini Mental State Examination, Beck’s depression inventory, and Beck’s anxiety inventory. The type and severity of aphasia were determined by the Boston Aphasia Test. Results: The highest number of patients, a total of 31 (37.3%), had moderate anxiety disorder. 30 patients (36.1%) had shown symptoms of moderate depression, and 33 (39.7%) had shown mild and moderate cognitive dysfunction. Conclusion: Anxiety, depression, and cognitive dysfunction were significantly more pronounced in subjects with aphasia.

To evaluate clinical characteristics and outcomes of urgent bronchoscopies due to foreign body aspiration (FBA), we analyzed cases from January 1954 to December 2021. The study included children up to 14 years of age who underwent emergency bronchoscopy on suspicion of a foreign body in the lower respiratory tract. Each patient was assessed for age, sex, nature and location of the foreign body, bronchoscopy findings, complications, and airway involvement. A total of 889 children underwent urgent bronchoscopy over 67 years. Most cases (66.7%) occurred within the first three years of life, and 10.6% involved children under one year of age. Recently, a marked decrease in positive findings in infants has been observed. Boys represented 62.5% of cases. Organic foreign bodies, predominantly pumpkin seeds, were most common. The right bronchus was the predominant location (51%). In the most recent period, multiple-location foreign bodies became more frequent. Both the annual number of bronchoscopies and the proportion of positive findings have declined. During the COVID-19 pandemic, suspicion of FBA and the number of bronchoscopies significantly decreased.

Sitagliptin is a dipeptidyl peptidase-4 (DPP-4) inhibitor used to treat type 2 diabetes. However, several studies have demonstrated its anti-inflammatory and immunomodulatory properties. The aim of this study was to investigate the effect of sitagliptin on the functional and phenotypic properties of human neutrophils under normal (NG, 5.5 mM)- and high (HG, 22 mM)-glucose conditions in vitro. Neutrophils were pretreated with varying concentrations of sitagliptin and stimulated with phorbol-12-myristate-13-acetate (PMA), N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP), calcium ionophore (CaI), or opsonized zymosan (OpZym). Survival, phenotypic, and functional characteristics were then assessed. Our results showed that sitagliptin was non-cytotoxic to neutrophils even at very high concentrations. It decreased the production of reactive oxygen species (ROS) and neutrophil extracellular traps (NETs), generally following a stimulus- and concentration-dependent pattern. The effect was more pronounced under HG conditions. Furthermore, sitagliptin showed a significant ROS-scavenging effect in a cell-free system. It also rapidly altered the expression of surface markers in both resting and fMLP-stimulated neutrophils, typically upregulating CD10, CD16, CD62L, CD63, CD88, CD89, and PD-L1, and downregulating CD11b/CD18, CD66b, and CD182, a phenotype consistent with a dampened, less-primed activation state of these cells. In conclusion, sitagliptin exhibited marked antioxidative/ROS-scavenging activity in neutrophil cultures and induced a coordinated shift in neutrophil phenotype, accompanied by suppression of NETosis under both NG and HG conditions. Collectively, these data support the view that neutrophils may constitute an additional cellular target contributing to sitagliptin’s anti-inflammatory and immunomodulatory profile.

Objective To compare cognitive profiles and dementia severity among older patients with atrial fibrillation (AF), with and without ischemic stroke (IS), and to evaluate the contribution of vascular burden to global cognitive status. Methods This cross-sectional clinical study included 124 patients aged ≥55 years who were stratified into three groups: AF without IS (n = 50), AF with IS (n = 25), and IS without AF (n = 49). Global cognitive status was assessed using ordinal categories derived from the Mini-Mental State Examination (MMSE). Attention and working memory were additionally evaluated using the Information-Memory-Concentration (IMC) test derived from the Blessed Dementia Scale. Vascular burden was assessed using the Hachinski Ischemic Score (HIS). Group differences were analyzed using appropriate statistical tests, and predictors of worse cognitive status were examined using ordinal logistic regression. Results Patients with combined AF and IS demonstrated a trend toward a less favorable cognitive profile and higher vascular burden compared with patients with AF alone or IS alone. The proportion of female participants differed significantly across groups (p = 0.022), whereas age category and educational level were comparable. In multivariable ordinal logistic regression analysis, higher Hachinski Ischemic Score independently predicted worse global cognitive status (OR 1.79, 95% CI 1.42–2.25; p < 0.001) after adjustment for age, sex, education, and major vascular risk factors. Conclusions Vascular burden plays an important role in cognitive impairment among older patients with atrial fibrillation, particularly when accompanied by ischemic stroke. Incorporating vascular burden assessment into routine clinical evaluation may facilitate earlier recognition and characterization of cognitive impairment in aging populations.

Simple Summary The virus responsible for COVID-19 can affect multiple animal species, including wildlife. In this study, we tested, by commercial ELISA, blood samples from red foxes and golden jackals in Serbia for IgG anti-SARS-CoV-2 antibodies. Antibodies were detected in both species, with a seroprevalence of 13.3% in red foxes and 7.3% in golden jackals (overall seroprevalence: 10.3%). All the samples were tested in parallel by an in-house adapted ELISA. These results provide the first evidence of SARS-CoV-2 exposure in wild canids in Serbia. Monitoring pathogens in wildlife improves our understanding of disease ecology and supports early recognition of potential zoonotic risks.

Background This study explored whether tumor regression following neoadjuvant therapy can be used as a reliable indicator of surgical operability in patients with stage IIIA non-small cell lung cancer (NSCLC). Methods A retrospective cohort analysis was performed, including patients with stage IIIA NSCLC treated at a tertiary thoracic surgery center. Patients were categorized according to treatment approach: induction therapy followed by surgery or primary surgical management. Treatment response was assessed using imaging findings, pathological staging changes, residual tumor burden, and lymph node status. Surgical feasibility and perioperative outcomes were evaluated. Statistical significance was defined at p<0.05. Results Patients receiving induction therapy demonstrated greater tumor reduction, higher rates of mediastinal nodal regression, and more frequent complete pathological response. Complete (R0) resection was achieved more often in this group. Tumor regression and nodal response were identified as independent predictors of surgical feasibility. Postoperative complication rates and mortality did not differ significantly between groups. Conclusion Tumor response after neoadjuvant therapy is closely associated with surgical operability in stage IIIA NSCLC. Response-based selection may improve resectability without increasing perioperative risk.

The search for new anticancer agents with improved efficacy and reduced toxicity has intensified interest in metal-based compounds. In this study, two novel palladium(II) complexes, synthesized from Schiff base ligands derived from 5-chloro-salicylaldehyde and p-hydroxybenzylamine or tyramine, were chemically characterized and biologically evaluated. Both complexes exhibited significant cytotoxic activity against the MCF-7 breast cancer cell line in a dose- and time-dependent manner, with Pd2 showing slightly higher potency. Morphological analysis of treated cells indicated that apoptosis is the predominant mechanism of cell death. To gain deeper insight into the potential mechanisms underlying the observed anticancer activity, several biologically relevant targets were investigated. Enzyme kinetics revealed that the complexes act as uncompetitive inhibitors of liver catalase, suggesting a possible role in the induction of oxidative stress. Fluorescence studies demonstrated that Pd2 interacts with CT-DNA through combined intercalative and minor groove binding modes and exhibits significant binding affinity toward human serum albumin, predominantly at Sudlow’s site I. Molecular docking analysis further supported favorable interactions with catalase, estrogen receptor α, and B-form DNA, providing structural insight into the experimentally observed biological effects. Overall, the study explores multiple potential mechanisms of anticancer action, underscoring the promising therapeutic potential of these palladium(II) complexes, while antitumor activity has been initially assessed using a MCF-7 cell line as a preliminary model.

Autori u radu pišu o političkoj situaciji u Banja Luci od 1990. do kraja 1992. godine. To je period od održavanja prvih višestranačkih izbora do perioda intenzivne kampanje protjerivanja i masovnih zločina u Banja Luci. Osim osvrta na rezultate izbora 1990., demografska slika opštine prema popisu stanovništva u Bosni i Hercegovini 1991. godine imala je veoma važnu ulogu u odnosu prema brojčano manjinskim narodima. U Banja Luci i cijeloj Bosanskoj krajini već su 1991. godine bile vidljive refleksije agresije Jugoslovenske narodne armije (JNA) na Hrvatsku, što je dovelo do zaoštravanja međunacionalnih odnosa. Izbijanjem agresije na Republiku Bosnu i Hercegovinu 1992. godine počinjeni su masovni zločini nad banjalučkim civilima bošnjačke i hrvatske nacionalnosti od strane Vojske Srpske Republike BiH (VSrRBiH) / Vojske Republike Srpske (VRS) i pripadnika policijskih struktura pri Ministarstvu unutrašnjih poslova (MUP) SrRBiH/RS. Terorisanje, zločini, prisilno odvođenje u logore i druga mjesta zatočenja te progon nad pripadnicima ova dva naroda nastavljeni su i nakon 1992. godine o čemu govore relevantni dokumenti i svjedočenja očevidaca.

Abstract Congenital intrinsic obstruction at or near the duodenojejunal junction is exceptionally rare and most commonly results from incomplete embryonic recanalization, leading to the formation of a mucosal web. We report a 7-day-old term male neonate (birth weight 3350 g) who presented with persistent feeding intolerance and intermittent bilious vomiting since birth. Abdominal radiography showed marked dilation of the stomach and duodenum with distal bowel gas. An upper gastrointestinal contrast study revealed a conical narrowing at the duodenojejunal junction. Surgical exploration revealed a mucosal web located immediately distal to the duodenojejunal junction. Given the marked luminal disparity, simple web excision was deemed inadequate, and segmental resection with primary end-to-end jejunojejunal anastomosis was performed. Postoperative recovery was uneventful. Proximal jejunal webs near the duodenojejunal junction are rare but surgically correctable causes of neonatal bilious vomiting and should be considered in the differential diagnosis.

Introduction Sepsis is a global health priority with nearly 50 million cases annually. Cardiovascular dysfunction is common, frequently manifesting as hypotension that persists despite fluid resuscitation. Most affected patients require the use of intravenous (IV) vasoactive agents, typically necessitating intensive care unit (ICU)-level monitoring, invasive interventions and contributing substantially to healthcare costs. Midodrine, an oral alpha-1 agonist approved for orthostatic hypotension, has increasingly been used off-label as a vasopressor-sparing (reducing IV vasopressor use) strategy in sepsis, despite limited and inconsistent evidence. This pragmatic, randomised, open-label trial evaluates the efficacy and safety of midodrine in patients with sepsis-associated hypotension. We hypothesise that, compared with standard care, midodrine administration will reduce the duration of IV vasopressor use. Methods and analysis A total of 308 adult patients with sepsis-associated hypotension will be enrolled (154 per arm). The intervention group will, in addition to standard of care, receive enteral midodrine 10 mg three times daily. Outcomes will be ascertained pragmatically via electronic health record-based data retrieval and adjudicated by research coordinators blinded to treatment assignment. The primary outcome is time alive and off IV vasopressors in the first 28 days (in hours) after randomisation. Secondary outcomes include cumulative vasopressor exposure; use and duration of central venous access; cumulative fluid balance over the first 48 hours and up to 7 days of ICU stay; ICU and hospital length of stay; and ICU-, hospital-, and organ support-free days through day 28. Safety outcomes include adverse events potentially attributable to midodrine during hospitalisation including acute kidney injury. Primary analyses will follow an intention-to-treat framework, including all randomised participants according to their assigned treatment groups. Primary and secondary outcomes will be compared using a van Elteren test stratified by randomisation factors. A predefined secondary Bayesian analysis of the primary outcome will provide complementary estimates of treatment effect. Safety outcomes will be summarised descriptively without formal between-arm hypothesis testing. Ethics and dissemination The Mayo Clinic Institutional Review Board approved this protocol and required written informed consent from all participants (IRB# 24–0 00 121). Findings will be disseminated through peer-reviewed publications and international conference presentations. Trial registration number NCT06319248.

In this paper, the dynamic analysis of a vibro-impact system with ideal excitation and various friction models is performed. A physical model of an oscillating mechanical system with possible impact occurrence is presented and the corresponding mathematical model is derived by using Lagrange’s equations of motion. To describe interaction between impact element and environment, three different friction models are considered: Coulomb, viscous and Coulomb-Stribeck model. Newton’s impact law with a coefficient of restitution is employed to describe relationship between pre-impact and post-impact velocities. The dynamic behavior of the vibro-impact system under the ideal excitation, where the system does not influence the excitation source, is investigated for each friction model by numerically solving the governing equations. The results of numerical analysis are presented through amplitude-frequency diagrams, displacement-time responses and phase portraits. The main objective is to determine the influence of different friction models on amplitude-frequency diagrams, particularly on the regions exhibiting impact and non-impact behavior. For parameter regions with multiple coexisting solutions, basins of attraction are constructed to illustrate the dependence of the system regime on initial conditions.

ABSTRACT Indoor spaces contain diverse microbial communities that shape human health. These microorganisms are particularly relevant to respiratory diseases, including asthma and allergies. Despite growing recognition of the importance of indoor microbial exposures, research in this field is slowed by differences in methods. These inconsistencies make it difficult to compare results and draw conclusions. This systematic review analyses 106 studies published between 2000 and 2025 that investigated indoor microbiomes in dust, air, and other matrices across homes, schools, and other built environments. We assessed sampling strategies, DNA extraction protocols, sequencing technologies, and bioinformatic pipelines, identifying trends, inconsistencies, and areas requiring harmonisation. Passive sampling, particularly dust collection, was the most common approach, while Illumina‐based 16S rRNA and ITS amplicon sequencing dominated molecular analyses. However, variations in targeted gene regions, extraction kits, and analytical tools limited cross‐study comparability. Ecological findings revealed consistent detection of bacterial taxa such as Staphylococcus, Streptococcus, and Corynebacterium, and fungal taxa including Cladosporium, Aspergillus, and Penicillium, with diversity shaped by building characteristics, ventilation, humidity, occupancy, and presence of pets. This review highlights the need for standardised protocols in indoor microbiome research to facilitate reproducibility, enable meta‐analyses, and inform health‐related guidelines for indoor environments.

BACKGROUND Schizophrenia (SCH) is a severe neuropsychiatric disorder associated with complex neurobiological alterations and suboptimal therapeutic outcomes. Increasing evidence indicates that mitochondrial dysfunction may contribute to SCH pathophysiology through impaired energy metabolism, oxidative stress, altered calcium homeostasis, and dysregulation of neuronal function. This review aims to summarize current knowledge regarding mitochondrial abnormalities in schizophrenia and to explore their potential involvement in the mechanisms of action of antipsychotic drugs. SUBJECTS AND METHODS A comprehensive review of the current literature was performed, focusing on human studies, cellular and animal models investigating mitochondrial alterations in schizophrenia, as well as studies evaluating the effects of antipsychotic medications on mitochondrial structure and function. RESULTS Available evidence demonstrates structural, molecular, and functional mitochondrial abnormalities in schizophrenia, including alterations in mitochondrial density, respiratory chain activity, oxidative phosphorylation, ATP production, and redox regulation. Antipsychotic drugs influence mitochondrial pathways in a complex and drug-specific manner. Some antipsychotics may impair mitochondrial respiration and increase oxidative stress, whereas others may exert compensatory or protective effects. Mitochondrial alterations may also contribute to cognitive impairment, negative symptoms, treatment response variability, and metabolic adverse effects associated with antipsychotic therapy. CONCLUSIONS Mitochondrial dysfunction represents an important component of schizophrenia pathophysiology and a potential therapeutic target. The interaction between mitochondrial pathways and antipsychotic drug effects highlights mitochondria as a possible pharmacodynamic interface for personalized treatment strategies. Further in vivo studies and biomarker-based approaches are required to clarify drug-mitochondria interactions and improve therapeutic outcomes in schizophrenia.