Celiac disease is a chronic gastroenterological disease, and a lifelong gluten-free diet is a needed andnecessary therapy, which implies the exclusion of products containing gluten from the diet. The declaration ofgluten-free products is often unclear and ambiguous. The purpose of this paper was to examine celiac diseasepatients' attitudes and experiences with gluten-free diet declarations. 95 respondents from the area of HNŽ with adiagnosis of celiac disease were included in this quantitative research. The anonymous survey was conducted usingthe Google Forms tool. An anonymous survey examined sociodemographic data (age, gender, education, duration ofillness), experiences and attitudes with declarations about gluten-free diet. In this research, more than half of therespondents are extremely careful about what they consume, without exception. Participants who have beendiagnosed with celiac disease for a longer period of time completely disagree to a greater extent that they do notknow what products they can consume. Individuals with gluten sensitivity may rely more on the 'gluten-free' claimfor information about the gluten content of a product. Individuals with celiac disease, on the other hand, may bemore experienced readers of food labels and may rely more on the ingredient list. Consumer expectations havedriven the food industry to continuously adapt and improve the formulations and processing techniques used in theproduction of gluten-free products.

Cellular death, aging, and tissue damage trigger inflammation that leads to enzymatic and non-enzymatic lipid peroxidation of polyunsaturated fatty acids present on cellular membranes and lipoproteins. This results in the generation of highly reactive degradation products, such as malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), that covalently modify free amino groups of proteins and lipids in their vicinity. These newly generated neoepitopes represent a unique set of damage-associated molecular patterns (DAMPs) associated with oxidative stress termed oxidation-specific epitopes (OSEs). OSEs are enriched on oxidized lipoproteins, microvesicles, and dying cells, and can trigger sterile inflammation. Therefore, prompt recognition and removal of OSEs is required to maintain the homeostatic balance. This is partially achieved by various humoral components of the innate immune system, such as natural IgM antibodies, pentraxins and complement components that not only bind OSEs but in some cases modulate their pro-inflammatory potential. Natural IgM antibodies are potent complement activators, and 30% of them recognize OSEs such as oxidized phosphocholine (OxPC-), 4-HNE-, and MDA-epitopes. Furthermore, OxPC-epitopes can bind the complement-activating pentraxin C-reactive protein, while MDA-epitopes are bound by C1q, C3a, complement factor H (CFH), and complement factor H-related proteins 1, 3, 5 (FHR-1, FHR-3, FHR-5). In addition, CFH and FHR-3 are recruited to 2-(ω-carboxyethyl)pyrrole (CEP), and full-length CFH also possesses the ability to attenuate 4-HNE-induced oxidative stress. Consequently, alterations in the innate humoral defense against OSEs predispose to the development of diseases associated with oxidative stress, as shown for the prototypical OSE, MDA-epitopes. In this mini-review, we focus on the mechanisms of the accumulation of OSEs, the pathophysiological consequences, and the interactions between different OSEs and complement components. Additionally, we will discuss the clinical potential of genetic variants in OSE-recognizing complement proteins – the OSE complotype - in the risk estimation of diseases associated with oxidative stress.

The research included seven autochthonous apple varieties in the area of Tomislavgrad, Stipanjići locality, during 2018, which represent the natural wealth of our country. In seven autochthonous apple varieties: Petrovača bijela, Budimka, Ljutika-Divljaka, Ledarica, Bedrica, Ljepocvjetka-Cvjetača and Jonatanka, phenological characteristics were monitored and analyzed with morphological-pomological properties of fruits. The following phenological characteristics were monitored: flowering phenophases, germination of the first fruits, fruit growth, fruit ripening and full maturity. Five characteristics for the description of apple fruits were determined using the UPOV descriptor for apples (UPOV, 2003). The results of monitoring the morphological and pomological properties of fruits show that the lowest average fruit weight was in the variety of Cvjetača (58,38 g), while the highest average of fruit weight had variety Bedrica (140,59 g). In the result is evident that the lowest value of fruit width had variety Cvjetača (53,72 mm), and the highest had variety of Jonatanka (70,83 mm). The Cvjetača variety had the lowest average fruit lenght (47,24 mm), while the Bedrica variety had the highest (59,72 mm). The Budimka variety had the highest average stem length (2,73 cm), while the Divljak variety had the lowest average stem length (1,43 cm). The Jonatanka variety had the largest average cup concavity and was (1,28 mm), while the Petrovača bijela variety (0,41 mm) had the lowest average cup concavity. Based on the this research, the examined autochthonous apple varieties can be used a valuable genetic material in breeding programs, and can be used to create new varieties, as well as for propagation in commercial plantations.

With a significant economic value, pepper (Capsicum annuum L., Solanaceae) represents a major vegetable crop worldwide. Simple sequence repeats (SSR) markers and expressed sequence tags (EST-SSR) are powerful tools for genetic studies and the breeding of pepper. In this study, the genetic variability among local pepper species was assessed by using 8 EST/SSR markers. The pepper varieties were collected locally, based on old sorts, hybrids, and sorts found in markets, with a total of 14 pepper varieties.  Mean expected heterozygosity (He) ranged from s 0.30 to 0.83 in the populations studied, whereas the mean polymorphic information content (PIC) was 0.62. The average polymorphic number of alleles per primer was 2.5 per locus. The genetic relationships among the populations revealed by Neighbor-Joining dendrogram showed a clear clustering to three sub-populations making distinct clusters.  As expected, all local varieties make up one cluster, together with the hybrid Istra, concluding that this hybrid evolved from the cross-breeding of local varieties.  These results indicate and confirm the regional and physiological differences between the pepper sorts.

Aims: The aims of the study were to analyse the polyphenols of Pulicaria dysenterica (L.) Bernh. methanolic extracts from aerial and underground parts, assessment of antioxidant activity and to evaluate their cytotoxicity on HeLa cells of cervical cancer. Methodology: The total phenolic content (TPC) of extracts was determined by the Folin-Ciocalteu spectrophotometric method. The qualitative and quantitative analysis of individual polyphenolic compounds were performed by the reverse phase HPLC method. The antioxidant capacity was evaluated by both, 2,2-diphenyl1-picrylhydrazyl radical and FRAP assay, while cytotoxicity of the extracts was assessed by MTT assay. Results: TPC of the samples were 127.62±2.22 and 244.12±8.84 mg gallic acid equivalent/g extract. In the extracts chlorogenic acid in amount of 10.06±0.96 and 11.32±0.28 mg/g, flavonoid rutin in amount of 5.68±0.13 mg/g and three caffeic acid derivatives were recorded. Extract from underground parts achieved better antioxidant activity with IC50 value 55.36±0.75 µg/mL and FRAP value 2411.12±37.22 µmol Fe2+g-1 compared to the one from aerial parts. Extract from aerial parts achieved better cytotoxic activity with 50% inhibition of viability (IC50) at concentration of 0.389±0.07 mg/mL, against HeLa cells, compared to the extract from underground parts. Conclusion: Analyzed Pulicaria dysenterica extracts contained phenolic acids and flavonoids. The extracts showed good antioxidant activity and cytotoxic properties against HeLa cells in vitro.

Traditional pear cultivars are increasingly in demand by consumers because of their excellent taste, the possibility of use in sustainable food production systems, convenience as raw materials for obtaining products of high nutritional quality, and perceived health benefits. In this study, individual sugars, organic acids, and polyphenols in the fruits of nine traditional and one commercial pear cultivar during two growing seasons were determined by HPLC. A significant influence of cultivars, growing years, and their interaction on the content of analyzed primary and secondary metabolites was determined. The commercial pear cultivar ‘Président Drouard’ and traditional cultivars ‘Dolokrahan’, ‘Budaljača’, and ‘Krakača’ had a lower content of all analyzed sugars. Overall, traditional pear cultivars had higher total polyphenols in the peel and pulp than ‘Président Drouard’, with the exception ‘Takiša’ and ‘Ahmetova’. High polyphenol content detected in ‘Budaljača’, ‘Dolokrahan’, and ‘Krakača’ shows the utilization value of traditional pear germplasm. The obtained data can serve as practical supporting data for the use of traditional pears in the neutraceutical, pharmaceutical, and food industries.

The student population is one of the most sensitive populations today, whereby it is necessary to consider the relationship between health and physical activity in order to improve students’ quality of life. In addition to physical inactivity, health status is adversely affected by constant technological innovations and excessive socializing, consumption of alcohol, tobacco and narcotics. The aim of this study was to determine the differences between the communication approach and attitudes of first- and fourth-year students of the Faculty of Sports and Physical Education on the impact of sports on health and improvement of mental hygiene. Using a 60-item questionnaire, the study included 70 healthy respondents of both genders (Mean ± SD: 20.86±1.76 years). The respondents were divided into two subsamples, i.e. a group of first-year students (IG; n=34; F=15, M=19) and a group of fourth-year students (IVG; n=36; F=18, M=18). All obtained data are presented by descriptive parameters. The reliability of the survey in terms of internal compliance was tested by Cronbach's Alpha coefficient. The manifest space of attitudes was processed by the scaling technique. The Mann-Whitney U Test was used to determine differences in attitudes between first- and fourth-year students. For statistical significance of the differences in results between groups, the p<0.05 level of significance was used. The data were processed in the SPSS statistical package. The results of the study unequivocally confirmed the existence of a positive attitude among the student population about the impact of sports on health and improvement of mental hygiene, as well as that there are no statistically significant differences in most of the variables included in the testing (eight out of ten domains), i.e. that there is no great difference in the attitudes of first- and fourth-year students on the importance of sports for their psychophysical health.

Contouring, planning and dose calculation in treatment planning systems (TPS) are based on computedtomography (CT) images. Therefore, it is important to have developed, optimized and adapted scanning protocolsfor specific anatomic regions and special radiotherapy modalities such as stereotactic radiosurgery (SRS). The aimof this study was to determine influence of tube voltage, field of view size (FOV) and reconstruction kernels on CTnumbers and the resulting radiotherapy (RT) dose calculation.This study was performed at Clinic of Oncology, Clinical Center University of Sarajevo. Verification electrondensity and CT number values was performed using CIRS Thorax 002LFC phantom, while anthropomorphic CIRS038 phantom for stereotactic end-to-end verification was used for the purpose of dose plan calculation analysis withlarge bore CT simulator Canon Aquillion LB.The significant correlation between the tube voltage and the measuredvalues of CT numbers is significant for all materials (p < 0.05), except for water (p = 0.310). No significantcorrelation between FOV and obtained values of CT numbers was found in any of the evaluated tissue equivalentmaterials. Evaluating the impact of reconstruction kernels on Hounsfield units (HU), significant deviations werefound for the FC62, FC68 and FC07 reconstruction kernels. Also, analyzing the influence of reconstruction kernelson the RT dose calculation, the extreme values are associated with Dmin/D in PTV for kernels FC41 and FC68, wheredeviations from the values obtained using the baseline scanning parameters were -1.3% and -1.9%. For deviation of1 HU in muscle tissue of CIRS 002LFC, the calculated Dmin/D in PTV of CIRS STEEV phantom will reduce by0.79%. Similarly, the reduction of D₉₈ and D₂ would be 6.8 cGy and 3.03 cGy for 1 HU, respectively. Change of thereconstruction kernels caused differences of 0.4% in Dmin/D calculation in clinical target volume (CTV).CT scanning and reconstruction parameters may affect Hounsfield units, which could have an impact on dosecalculations in RT plan. Hence, it is recommended to standardize the scanning protocol used in calibration curvegeneration for TPS. One should avoid use of different tube voltages and kernels, while according to this study, thechange of FOV will have no impact on dose calculations

Over the past decades, recognised importance of prompt and valid pain recognition and quantification in veterinary medicine significantly increased interest in investigating attitudes and self-rated abilities of veterinarians towards pain assessment. However, giving that the owners are the ones who decide when to call the veterinarian, it is also essential to investigate their attitudes and knowledge regarding pain recognition and management. This is the first research investigating horse owners’ attitudes and self-rated abilities towards pain assessment and management in Bosnia and Herzegovina. The participants were invited via email or social media with attached link to online questionnaire created using the Google Forms platform. Surveyed population included adult sport or pleasure horse owners, and horse caretakers in Bosnia and Herzegovina. Study response rate was 33.33% (40 respondents). The questionnaire consisted of sections asking about respondents’ demographic data, general pain assessment and management, and attitudes towards pain assessment done by veterinarians compared with theirs. Study respondents considered their abilities for pain recognition and quantification as sufficient and sufficient/moderate, even though most of them were unfamiliar with pain scales, and only negligible number use them. This study indicates the need and importance of owners' education concerning pain recognition and quantification in horses, and their more effective communication with veterinarians.

The way agri-food companies conduct business has changed as a result of changes in the market. These companies must start working in a more environmentally friendly manner. This study aims to examine, assess, and compare how various fuzzy methodologies are applied in green supplier selection (GSS), using an agri-food industry as an example. The company Biljana Brko, which engages in GSS, was observed in this study. The selection aids in the acquisition of raw materials and materials whose environmental impact will be minimized. Ecological and economic factors were taken into consideration when choosing green suppliers. Experts who assessed the weight of the criteria and the suppliers with linguistic values were chosen to carry out this selection. In order to do this, a fuzzy set that effectively applies these linguistic values was employed. The fuzzy SWARA (FSWARA) approach was utilized to calculate the weights of the criteria, revealing that the criterion of Environmental Management System has the highest weight. Drawing on the opinions of experts, suppliers were ranked using the fuzzy MABAC, MARCOS, and CRADIS techniques. The results show that supplier S2 receives the highest ratings. Along with this provider, supplier S3 is noteworthy because it excelled in the sensitivity analysis across a variety of scenarios. In light of this, Biljana Brko should give preference to these suppliers. Further, the results of the three adopted techniques were compared. The comparison reveals that the ranking order produced by all three techniques is remarkably similar. This supplier order differed slightly from the FMABAC method just in one scenario. Hence, this work demonstrates that the three fuzzy techniques can solve the GSS problem and other problems by ranking alternatives.

Writing is an integral part of linguistic education of deaf persons which, due to the lack of auditorysensors, has an insurmountable difficulty achieving, so this shortcoming is directly reflected in the general literacyof deaf children. Many detailed studies in the world suggest that in order to become literate, a deaf child must learnthe language of the community. Je/she must first learn the letters and learn to write. About 50% of young deafpeople after high school read and write worse than a ten-year-old hearing child. The aim of this research is toexamine the usage, reading, writing, and comprehension in deaf children of the adverbial clause of place in writtenform and to determine the statistical significance of differences between deaf and hearing children. The study wasconducted on a sample of 140 respondents. The first subsample of subjects, the experimental group consisted of 70deaf students, and the second subsample, a control group of 70 hearing students, of the same chronological age. Themeasurement instrument “Test of writing, and comprehension of an adverbial clause of place” was applied. Thefrequencies and percentages of respondents' responses to the variables were calculated. The F (Fisher) test was usedto determine the statistical significance of the differences between deaf and hearing subjects. The results showed that1.40 % of deaf children are completely successful in reading and writing priloske odredbe, and thaat 90 % of deafchildren use prislocke odredbe partially successfully. The percentage of children that do not use priloske odredbe inany form in their written communication is 8.60%. The order of use of adverbial clause of place by frequency is asfollows: "in", "on", "below", "behind", "next to", and "between". The largest number of deaf children use theadverbial clause of place "in" in their written expression, 65.70% of them. Then, the adverbial clause of place "on"51.14%, the adverbial clause of place "below" 47.14%, the adverbial clause of place "behind" 31.43%, the adverbialclause of place "beside" 30%, and the adverbial clause of place "between" 22.85%.There is a statistically significant difference between deaf and hearing children in the use of the adverbial clause ofplace at the level of statistical significance of p=0.000.