Background The main goal of the paper is to review the existing state and propose a model solution for the introduction of the waste-to-energy concept in the Republic of Serbia and Bosnia and Herzegovina, as these Balkan countries are a source of high pollution due to the inefficient use of fossil fuels and the operation of coal-fired power plants. Besides, these countries have very low level of waste management, which results in the uncontrolled disposal of a large amount of waste which consists of plastic and microplastic materials which are difficult to decompose in natural ecosystems. Methods Considering the type and objectives of the study, a mixed research method was chosen as a combination of exploratory research, descriptive research, explanatory research, and modeling. Results The main result of the research shows the unfavorable situation in the field of waste management in the Republic of Serbia, as well as in Bosnia and Herzegovina. Both countries have adopted adequate strategies and plans, but waste management is not implemented accordingly, Nevertheless, numerous problems create considerable opportunities for improvement, especially regarding the potential for energy production from waste, which is an important approach to implementing the circular economy model. The study showed that the situation is particularly unfavorable in rural areas. Hence, the research proposes (a) a novel model for waste-to-energy governance and (b) a novel model for waste-to-energy management in rural areas. The research was done, and models were developed based on the examples of the Republic of Serbia, and Bosnia and Herzegovina. However, the results can be used in countries with a similar level of waste management and with a larger share of rural areas. Conclusions The paper emphasizes the importance of a holistic and systemic approach to waste management, with emphasis on using waste-to-energy concept as particularly applicable in the transition to circular economy. This study proposes a model for the integration of waste management (with emphasis on plastic and microplastic) and energy efficiency, presenting a model of approach that can be used in countries that are at the beginning of introducing a circular economy.

Narcissus poeticus L. (Amaryllidaceae), a facultative serpentinophyte, is a highly variable species and particularly important ancestor of cultivated daffodils, but is rarely studied in field populations. This study, based on natural populations in the Balkans, focused on karyotype variability, genome size, ploidy and the presence of B chromosomes. Thirteen native populations from different environmental and soil conditions were collected and analyzed using flow cytometry to estimate nuclear genome size, fluorescence in situ hybridization (FISH) for physical mapping of rDNA, fluorochrome labeling (chromomycin and Hoechst) for heterochromatin organization and silver nitrate staining of nucleoli for determining rRNA gene activity. The organization of rDNA and natural triploids is reported here for the first time. The presence of individuals with B chromosomes (in 9/13 populations) and chromosomal rearrangements was also detected. The observed B chromosome showed three different morphotypes. The most frequent submetacentric type showed four different patterns, mainly with active ribosomal genes. The results obtained show that N. poeticus has a dynamic genome with variable genome size due to the presence of polyploidy, B chromosomes and chromosomal rearrangements. It is hypothesized that the observed changes reflect the response of the genome to different environmental conditions, where individuals with B chromosomes appear to have certain adaptive advantages.

Abstract The olive trituration activity is one of the most important industrial activities in Fez, Morocco. These effluents are highly loaded with organic, inorganic, and phenolic compounds without any preliminary treatment that affects water quality. In this sense, the waters of Oued Fez are deteriorating due to the discharge of wastewater from oil mills and the excessive proliferation of Pistia stratiotes L., an invasive macrophyte that represents a significant stress to the aquatic ecosystem, eutrophication, and a reduction in biodiversity. This ecological situation has prompted us to carry out phytotoxicity bioassays based on the detection of the lethal concentration of P. stratiotes in the wastewater from the olive oil mill to evaluate the sensitivity and tolerance of macrophytes to the different concentrations of total polyphenols (TPP) present in the olive mill wastewater. To estimate their impact on the environment. Fresh whole plants of P. stratiotes were exposed to varying concentrations of olive oil mill wastewater with a series of TPP concentrations (0–30 mg/l) for 1 week in the natural environment. The results also show that P. stratiotes is able to grow rapidly in culture with 20 mg/l of TPP; the highest growth of wet weight of P. stratiotes occurred at 20 mg/l treatment with an average of 13 g wet weight increase. At 0 mg/l, treatment is known to cause very slow growth with an average of 2 g. Olive oil mill wastewater was toxic to the plant at concentrations higher than 30 mg/l, and the phytotoxic effect was manifested by retardation of growth, detachment of roots, wilting, and chlorosis of leaves. This indicates that polyphenols have great potential to inhibit the proliferation of P. stratiotes in aquatic environments.

Introduction: There is considerable concern with rising rates of antimicrobial resistance (AMR) with its subsequent impact on morbidity, mortality and costs. In low- and middle-income countries, a key driver of AMR is the appreciable misuse of antibiotics in ambulatory care, which can account for up to 95% of human utilisation. A principal area is the selling of antibiotics without a prescription. There is conflicting evidence in South Africa regarding this practice alongside rising AMR rates. Consequently, there is a need to explore this further, especially in more rural areas of South Africa. A pilot study was undertaken to address this. Materials and Methods: A two-step descriptive approach involving a self-administered questionnaire amongst pharmacists and their assistants followed by cognitive interviews with some of the participants. Results: Twenty-one responses were obtained from nine of the 11 community pharmacies invited to participate. Participating pharmacies were all independently owned. Ten of the 21 participants admitted dispensing antibiotics without a prescription, including both adults and children, representing five of the nine participating pharmacies. A minority dispensed antibiotics before recommending suitable over-the-counter medicines. These high rates were exacerbated by patient pressure. There were issues with the length of the questionnaire and some of the phraseology, which will be addressed in the main study. Conclusion: There were concerns with the extent of purchasing antibiotics without a prescription in this pilot in South Africa study. Key issues will be explored further in the main study.

Abstract The impact of logistics performance in the era of sustainable mobility on the overall economic development of a country is inevitable. It can even be said to represent an extremely important component in identifying economic conditions and provides the possibility of defining adequate strategies. In this article, the evaluation of the member countries of the European Union was carried out on the basis of the logistics performance index (LPI) according to the latest report of the World Bank (WB). A unique and original Multiple-Criteria Decision Making (MCDM) approach has been created, and it involves the application of four methods: Criteria Importance Through Intercriteria Correlation, Method based on the Removal Effects of Criteria, and Entropy and Fuzzy ROV (Range of Value). The weighting coefficients of six factors were obtained with the first three methods in crisp form, so they were converted into Triangular Fuzzy Number. The Fuzzy ROV method has been created for the first time in the literature and represents a great contribution from the methodological aspect. The results of the developed model and the applied steps show that there are certain differences in the rankings compared to the World Bank report, with a note that the best-ranked countries have maintained their positions. In addition, verification tests of the originally obtained results were created, with an emphasis on the importance of evaluation parameter values and their impact on the LPI ranking.

Financial performance analysis is of vital importance those involved in a business (e.g., shareholders, creditors, partners, and company managers). An accurate and appropriate performance measurement is critical for decision-makers to achieve efficient results. Integrated performance measurement, by its nature, consists of multiple criteria with different levels of importance. Multiple Criteria Decision Analysis (MCDA) methods have become increasingly popular for solving complex problems, especially over the last two decades. There are different evaluation methodologies in the literature for selecting the most appropriate one among over 200 MCDA methods. This study comprehensively analyzed 41 companies traded on the Borsa Istanbul Corporate Governance Index for 10 quarters using SWARA, CRITIC, and SD integrated with eight different MCDA method algorithms to determine the position of Turkey's most transparent companies in terms of financial performance. In this study, we propose "stock returns" as a benchmark in comparing and evaluating MCDA methods. Moreover, we calculate the "rank reversal performance of MCDA methods". Finally, we performed a "standard deviation" analysis to identify the objective and characteristic trends for each method. Interestingly, all these innovative comparison procedures suggest that PROMETHEE II (preference ranking organization method for enrichment of evaluations II) and FUCA (Faire Un Choix Adéquat) are the most suitable MCDA methods. In other words, these methods produce a higher correlation with share price; they have fewer rank reversal problems, the distribution of scores they produce is wider, and the amount of information is higher. Thus, it can be said that these advantages make them preferable. The results show that this innovative methodological procedure based on 'knowledge discovery' is verifiable, robust and efficient when choosing the MCDA method.

The mucosal pellicle (MP) is a biological film protecting the oral mucosa. It is composed of bounded salivary proteins and transmembrane mucin MUC1 expressed by oral epithelial cells. Previous research indicates that MUC1 expression enhances the binding of the main salivary protein forming the MP, MUC5B. This study investigated the influence of MUC1 structure on MP formation. A TR146 cell line, which does not express MUC1 natively, was stably transfected with genes coding for three MUC1 isoforms differing in the structure of the two main extracellular domains: the VNTR domain, exhibiting a variable number of tandem repeats, and the SEA domain, maintaining the two bound subunits of MUC1. Semi-quantification of MUC1 using dot blot chemiluminescence showed comparable expression levels in all transfected cell lines. Semi-quantification of MUC5B by immunostaining after incubation with saliva revealed that MUC1 expression significantly increased MUC5B adsorption. Neither the VNTR domain nor the SEA domain was influenced MUC5B anchoring, suggesting the key role of the MUC1 N-terminal domain. AFM-IR nanospectroscopy revealed discernible shifts indicative of changes in the chemical properties at the cell surface due to the expression of the MUC1 isoform. Furthermore, the observed chemical shifts suggest the involvement of hydrophobic effects in the interaction between MUC1 and salivary proteins.

Abstract The banking sector assumes a pivotal role in the economic development of nations. The assessment of financial indicators pertaining to banks holds fundamental importance in the evaluation of bank stability and sustainability. This research employs the MEREC (Method based on the Removal Effects of Criteria) and MARCOS (Measurement of Alternatives and Ranking according to COmpromise Solution) methodologies to delve deeper into the financial landscape of the banking sector in Bosnia and Herzegovina (BiH). Specifically, the objective is to rank banks according to their financial indicators, utilizing financial data from the year 2022. The MEREC method is applied to gauge the significance of financial indicators and ascertain their respective weights, while the MARCOS method is employed to rank banks within BiH based on their financial indicators. The examination of financial indicators within the BiH banking sector, facilitated by the MEREC and MARCOS methodologies, yields a more comprehensive understanding of the sector’s present condition. Limitations of this research, which primarily stem from its reliance on available financial data and predefined methodologies, lies within limited consideration for external factors. To provide a broader contextual perspective, the inclusion of additional financial indicators and comparative analyses with banking sectors of other nations would be imperative. The findings of this research reveal that Raiffeisen Bank exhibits the most favourable financial indicators and demonstrates the highest level of efficiency within this context. Consequently, this research offers insights into identifying exemplary banks that can serve as models for enhancing the performance of others.

Haemonchus contortus is a globally significant parasitic nematode in ruminants, with widespread resistance to benzimidazole due to its excessive and prolonged use. Given the extensive use of benzimidazole anthelmintics in Bosnia and Herzegovina, we hypothesized that resistance is prevalent. The aim of this study was to identify the presence of anthelmintic resistance to benzimidazole in H. contortus from naturally infected sheep, goats and cattle in Bosnia and Herzegovina through the detection of the Phe/Tyr polymorphism in the amino acid at position 200 of the β-tubulin protein. From 19 locations in Bosnia and Herzegovina, a total of 83 adult H. contortus were collected from the abomasum of ruminants. Among these, 45 H. contortus specimens were isolated from sheep, 19 from goats and 19 from cattle. Results showed that 77.8% of H. contortus in sheep exhibited homozygous resistant genotypes at position 200 of the β-tubulin gene, with 15.5% being heterozygous. In goats, all tested H. contortus (100%) were homozygous resistant, and no heterozygous resistant or homozygous sensitive genotypes were found. Cattle had 94.7% homozygous resistant H. contortus, with no heterozygous resistant genotypes detected. In H. contortus from sheep and cattle, 6.7% and 5.3%, respectively, displayed homozygous sensitive genotypes. This study, for the first time, highlights the presence of a resistant population of H. contortus in sheep, goats and cattle in Bosnia and Herzegovina, using the rt-qPCR method. The resistance likely spread from sheep or goats to cattle, facilitated by shared pastures and the practice of transhumance, indicating a widespread and growing issue of anthelmintic resistance.