Ants are emerging model systems to study cellular signaling because distinct castes possess different physiologic phenotypes within the same colony. Here we studied the functionality of inotocin signaling, an insect ortholog of mammalian oxytocin (OT), which was recently discovered in ants. In Lasius ants, we determined that specialization within the colony, seasonal factors, and physiologic conditions down‐regulated the expression of the OT‐like signaling system. Given this natural variation, we interrogated its function using RNAi knockdowns. Next‐generation RNA sequencing of OT‐like precursor knock‐down ants highlighted its role in the regulation of genes involved in metabolism. Knock‐down ants exhibited higher walking activity and increased self‐grooming in the brood chamber. We propose that OT‐like signaling in ants is important for regulating metabolic processes and locomotion.—Liutkevičiūtė, Z., Gil‐Mansilla, E., Eder, T., Casillas‐Pérez, B., Di Giglio, M. G., Muratspahić, E., Grebien, F., Rattei, T., Muttenthaler, M., Cremer, S., Gruber, C. W. Oxytocin‐like signaling in ants influences metabolic gene expression and locomotor activity. FASEB J. 32, 6808–6821 (2018). www.fasebj.org

The paper presents an online two-phase business process model-driven generator of the conceptual database model. The generator is implemented as a web-based, platform-independent tool, in contrast to the existing tools that are dependent on some specific technological platform used for their implementation. Unlike the existing approaches, which are characterised by the direct synthesis of the target model based on business process models represented by a sole concrete notation such as BPMN, the presented generator uses an indirect two-phase approach, which is based on the introduction of a simple domain specific language as an intermediate layer between source and target notations. The implemented online generator enables automatic generation of the target data model represented by UML class diagram, based on business process models represented by two concrete notations: BPMN and UML activity diagram.

OBJECTIVE This study sought to obtain a comprehensive, reliable and valid instrument for evaluation of the presence of dental fear and anxiety (DFA) in children, through evaluation of the reliability and validity of three modified versions of the Children's Fear Survey Schedule-Dental Subscale (CFSS-DS). MATERIALS AND METHODS The study sample comprised children aged 9, 10, 11 and 12 years. The first sample group (200 patients) filled in a modified version of the CFSSDS scale, the second sample group (100 patients) filled in a short form of the Dental Fear Survey Schedule, and the third sample group (100 patients) filled in a short version of the CFSS-DS scale, prior to dental treatment, respectively. In order to determine test-retest reliability, the 184 patients from the first sample group filled in the modified version of the CFSS-DS scale again, prior to their next scheduled dental appointment. RESULTS The modified version of the CFSS-DS scale had the best internal consistency reliability (α=0.907), as well as validity results, compared to the other two instruments used. Test-retest reliability was moderate (Intraclass correlation coefficient: 0.58). CONCLUSIONS Of the three psychometric instruments used for evaluation of DFA presence in children, the modified version of the CFSS-DS scale showed the most clinically adequate reliability and validity values. This study thus provides a new psychometric instrument that should be considered for clinical use in evaluation of DFA presence in 9-12 year-olds, in a clinical setting sample type of children.

Incidence of breast cancer ranges from 27 per 100,000 in Middle Africa and Eastern Asia to 92 per 100,000 in Northern America. It is the fifth most common cause of death from cancer in women, with an estimated 522,000 deaths per year (6.4% of the total). Autosomal dominant inheritance of these cancers is characterized by transmission of cancer predisposition from generation to generation, with around 5-10% of all breast cancers being associated with inherited mutations in BRCA1, BRCA2 and other genes.  Breast and ovarian cancers are strongly associated with BRCA1 and BRCA2 mutations. In this study, we genotyped BRCA1 gene for large genomic rearrangements in breast and ovarian cancer patients from Bosnia and Herzegovina, with aim to assess frequency of large BRCA1 mutations (exon deletions/duplications) in this group. We collected 59 breast cancer samples, as well as other data concerning patients’ histopathological parameters of tumor, like age at diagnosis, cancer type, TNM class, cancer grade, as well as estrogen, progesterone and Her2/neu expression. Following DNA extraction from breast cancer samples (tissue after biopsy), BRCA1 mutations were identified by Multiplex Ligase - Dependent Probe Amplification (MLPA) analysis. Biostatistical analyses were conducted using MedCalc v.9.2.0.0 software. In all statistical tests p<0.05 was considered significant. Mean age at diagnosis was 54±1.75 (range 17 – 80). BRCA1 genomic rearrangements were found in 22% of breast and ovarian cancer patients. Statistically significant associations and correlations were found between BRCA1 genomic rearrangements and cancer type, estrogen, progesterone and Her2/neu expression, but not cancer grade, size, invasiveness or patients’ age

In this paper we aim to add additional knowledge regarding the occurrence, origin and epidemiological features of the English sweating sickness. The English sweating sickness raged in five devastating epidemics with mortality rates between 30 and 50% between 1485 and 1551 throughout England, and on one occasion also affected mainland Europe, in 1529. The Picardy sweat, generally considered as the English sweating sickness' lesser deadly successor, flared up in France in 1718 and caused 196 localized outbreaks with varying severity all over France and neighboring countries up to 1861. The English sweating sickness has been the subject of numerous attempts to define its origin, but so far all efforts have failed due to lack of material, DNA or RNA, that - using modern techniques and knowledge - could shed light on its cause. Although the time frame in which the English sweating sickness occurred and the geographical spread of the outbreaks is generally known, we will demonstrate here that there was more to it than meets the eye. We found reports of cases of sweating sickness in years before, after and between the 1485, 1508, 1517, 1529 and 1551 epidemics, as well as reports of sweating sickness in Italy and Spain. CONCLUSION In spite of the fact that the English sweating sickness apparently has not caused casualties for a more than a century now, we suggest that -given the right circumstances- the possibility of re-emergence might still exist. The fact that up until today we have no indication concerning the causal pathogen of the English sweating sickness is certainly not re-assuring.

Making a decision in everyday life always comes with uncertainty and responsibility. To reduce the risk to a minumum and in order to make a right decision a person can use methods of multicriteria analysis in combination in fuzzy logic. A married couple, representing decision makers in this case study, have purchased an apartment and it needs to be completely refurbished including outside carpentry. Aim of this study is to select the most suitable manufacturer of PVC carpentry for apartment refurbishing. A total pool of 14 quantitative and qualitative criteria is used as a base for selection of the most suitable of seven available manufacturers. For this case study we will use one of newer methods of multicriteria analysis of fuzzy Evaluation Based on Distance from Average Solution (fuzzy EDAS) method. Having reached the results, an analysis of sensitivity has been conducted showing stability of results where manufacturer number 4 represents an optimal solution in 13 experimental sets out of 14 total. DOI: http://dx.doi.org/10.5755/j01.ee.29.3.16818

Financial Reynolds number works as a proxy for volatility in stock markets. This piece of work helps to identify the predictability and herd behavior embedded in the financial Reynolds number (time series) series for both CNX Nifty Regular and CNX Nifty High Frequency Trading domains. Hurst exponent and fractal dimension have been used to carry out this work. Results confirm conclusive evidence of predictability and herd behavior for both the indices. However, it has been observed that CNX Nifty High Frequency Trading domain (represented by its corresponding financial Reynolds number) is more predictable and has traces of significant herd behavior. The pattern of the predictability has been found to follow a quadratic equation.

Data mining and clustering are important elements of various applications in different fields. One of the areas were clustering is rather frequently used is web intelligence, which nowadays represents an important research area. Data collected from the web are usually very complex, dynamic, without structure and rather large. Traditional clustering techniques are not efficient enough and need to be improved. In this paper, we propose combination of recent swarm intelligence algorithm, bare bones fireworks algorithm, and k-means for clustering web intelligence data. The proposed method was compared with other approaches from literature. Based on the experimental results, it can be concluded that the proposed method has very promising characteristics in terms of the quality of clustering, as well as the execution time.

OBJECTIVE The aim of this study was to evaluate an ongoing outbreak of brucellosis in southern region of Bosnia and Herzegovina (BIH) on the epidemiological, clinical and molecular level. PATIENTS AND METHODS This study included 19 patients affected by brucellosis between 2015 and 2017, in Trebiševo (BIH). Out of 19 patients, 16 were admitted to and treated at the Department of Infectious diseases of the University Clinical Hospital Mostar, while three patients were treated in ambulatory care setting. Epidemiological, clinical and microbiological parameters were investigated. The Rose Bengal test (RBT) positive sera were serologically confirmed by complement fixation test (CFT). We also analyzed blood cultures, and isolates were additionally serotyped. Molecular analyses were performed with Bruce-ladder multiplex polymerase chain reaction (PCR) and multiple locus variable number of tandem repeat analysis of 16 loci (MLVA-16) assay. RESULTS Fifteen out of 19 patients had been professionally exposed to the bacterium, while four patients acquired brucellosis without prior contact with infected animals. In seven out of eight (87.5%) patients with localized form of brucellosis, we detected significantly higher values of C-reactive protein (CRP) or erythrocyte sedimentation rate (P<0.001). B. melitensis was isolated from 13/16 (81.3%) blood culture samples, and additionally serotyped as biovar 3. Using MLVA16 assay, 11 isolates were genotyped. We observed complete genotype matches among 8/11 B. melitensis isolates, while 3/11 isolates differed in Bruce04 locus. CONCLUSION Overall, our study confirms the usefulness of MLVA-16 method in the epidemiological and molecular research of brucellosis during epidemic that, most likely, originated from the same source.

Earlier research has shown that students have tremendous difficulties with understanding certain aspects of rolling without slipping, such as the zero-velocity at the contact point and plausibility of application of the law of conservation of mechanical energy despite action of the friction force. The aim of this research was to explore whether using analogies and reasoning about extreme cases can facilitate conceptualization of the above-mentioned phenomena. A pre-test – post-test quasi-experiment has been conducted, with 93 students in the control group (CG) and 91 students in the experimental group (EG). Whereas control group students received conventional teaching, in the experimental group rolling of a cylinder has been considered as a special case of a tumbling prism for which the number of prism surfaces tended to infinity. The results of analysis of covariance showed that students from the experimental group significantly outperformed their peers from the control group on the Rolling Motion Concept Test (RMCT). Between-group differences were greater on test items that required higher level of cognitive transfer. This research suggests that using analogies and extreme case reasoning can facilitate comprehension of certain seemingly counterintuitive aspects of rolling motion. Keywords: analogy-based teaching, energy conservation, extreme case reasoning, misconceptions, rolling motion.

This paper presents the medical journals published in Bosnia and Herzegovina (BIH) in the period from 1878 to 1945. The first medical journal in BIH may be deemed to be Jahrbuch des Bosnisch-Hercegowinischen Landesspittales in Sarajevo (The Yearbook of the National Hospital of Bosnia and Herzegovina in Sarajevo). In a special part of this journal, doctors from Austro-Hungary serving in Sarajevo wrote scholarly articles about their patients' various ailments. Up to 1945 seven more medical journals were published in BIH: Trezvenost - Organ Jugoslavenskog Saveza Trezvenosti (Temperance - the Journal of the Yugoslav Temperance Society), Zdravlje - Lekarske pouke o zdravlju i bolesti (Health, Medical lessons on Health and Disease), Glasnik Lekarske komore za Bosnu, Hercegovinu, Dalmaciju i Crnu Goru (The Journal of the Chamber of Physicians of Bosnia, Herzegovina, Dalmatia and Montenegro), Glasnik Lekarske komore Vrbaske banovine (Journal of the Chamber of Physicians of Vrbaska banovina, Glasnik Lekarske komore Drinske banovine (Journal of the Chamber of Physicians of Drinska banovina), Vjesnik Zavoda za suzbijanje endemijskog sifilisa u Bosni i Hercegovini (Journal of the Institute for Combatting Endemic Syphilis in Bosnia and Herzegovina) and Časopis za medicinu i biologiju (Journal for Medicine and Biology). CONCLUSION Medical journals published in BIH in the period from 1878 to 1945 were published in times marked by specific political and social circumstances in BIH, in the time when BIH was not independent, and was under the influence of the health culture of the ruling regimes. Most of the authors of the articles published in these journals were citizens of the occupying authorities, although the papers published were mainly the result of research undertaken in BIH.

Phase-Shifting Transformers (PST) are installed in order to block or facilitate certain power flows. The installation of a single device affects power flows in a wider geographical area. Therefore, determining the optimal location for the Phase Shift transformer is of the utmost importance. Firstly, this paper presents summarizes the key factors for the installation of Phase Shift Transformers. Secondly, it give the prerequisites for the installation and finally, and thirdly it determines the deduced critical factors which have to be analyzed from the local (substation level), and global (system-wise) perspective. Given the necessity for regional approach when determining optimal PST location, this paper focuses on selected results of possibilities for active power flow control using PSTs in the South Eastern Europe (SEE) transmission grid. At the end, in the form a a power flow anaylsis, such an example is presented.