In this study the efficacy of short tandem repetitive sequences - STR was examined to prove kinship among the descendants of one parent pairs within the small local population by analyzing sharing of alleles per locus at siblings and non-siblings of the two villages: Vukotići and Orahovica and comparing these results with the results of a heterogeneous Bosnian-Herzegovinian population of our previous research. There was a significant difference in this division in the group of siblings and a group of non-siblings as well and the phenomenon of polarization when it comes to the representation of sharing zero and two alleles in the samples for both villages, with significantly overrepresented sharing of zero alleles at non-siblings and significantly overrepresented sharing of two alleles at siblings. By comparing sharing of alleles between sample from the small local population and the sample from mixed population from our earlier research, there is no statistically significant difference in the distribution of 2, 1 and 0 alleles between samples of these two population. This obtained ratio of dividing 2, 1 and 0 alleles in these two samples is about that both samples belongs to the same Bosnian-Herzegovinian population. and in the village of Vukotići. One of the possible reasons is the fact that Vukotići is less than the Orahovica. Using STR system in determining the kinship, or lack of the kinship in a small local populations within the Bosnian-Herzegovinian population, is proved to be a successful method based on the results of this research.

Introduction: Many epidemiological studies have shown that there are numerous risk factors for acute coronary disease. The aim is to determine the effect of risk factors on the echocardiographic changes and quality of life in patients treated with different methods 1 year after myocardial infarction. Methods: The research was a prospective–retrospective, clinical, epidemiological study and was conducted at the Clinic of Cardiology, University Clinical Center Sarajevo. Patients were divided into four groups based on the therapy treatment they got. The patients were divided into four groups based on the therapy treatment they received. The first group consisted of 40 patients who had had myocardial infarction and were treated with medications. The patients in the groups II and III were treated with percutaneous coronary intervention (PCI) [who immediately after incident underwent primary PCI or delayed PCI], and each group consisted of 40 patients. The group IV consisted of 40 patients, who underwent surgical revascularization (coronary artery bypass surgery). After the treatments have finished, an echocardiogram was performed on every patient. The Short Form (SF)-36 health survey was used for testing the life quality. Echocardiogram and the quality of life (QoL) testing were repeated a year after the treatment. Results: The study included 160 patients with a history of myocardial infarction, of which 130 (81.3%) were men, and 30 (18.8%) were women. The average age in the total sample was 54.9 ± 8.8 years. The review of risk factors’ presence showed that in the total sample, most present was hypertension with 134 (83.8%), smoking with 120 (75.0%), and hypercholesterolemia with 110 or 68.8% of patients. Hypertension showed a statistically significant negative effect on the SF-scales only in the group III according to the mental health (P = 0.020), social functioning (P = 0.013), and pain (P = 0.011). A statistically significant effect of smoking was observed in the group III according to left ventricular internal dimension in end-diastole (P = 0.000) and left ventricular internal dimension in end-systole (P = 0.001) in the sense that smokers have the higher values of these parameters, and negative to ejection fraction (EF) (P = 0.001) in the sense that smokers have lower EF. In the group IV, positive correlation was observed to EF (P = 0.038), and negative toward the mitral regurgitation (P = 0.032). Conclusion: High blood pressure negatively affected the QoL. Smoking is negatively associated with all observed echocardiographic parameters in all the groups except with the size of the left atrium.

I ntroduction : Genotoxic effect of ceftriaxone, a antibiotic of the third generation cephalosporins, have been evaluated. Metods: In vitro analysis included evaluation of the genotoxic and cytotoxic potential of different test ceftriaxone in concentrations of 0.15, 0.25 and 0.50mg/mL. Genotoxic potential was evaluated by using the cytokinesis block-micronucleus cytome assay in cell of cultivated human peripheral blood lymphocyte cells. Results: Results of the analysis on the presence of micronuclei, nuclear buds and nucleoplasmic bridges in 1000 binuclear cells per sample showed that the relative frequency of these indicators increased with increasing concentrations of ceftriaxone in lymphocyte cultures, while there are significant differences in their frequencies relative to controls as determined for each ceftriaxone concentration. Conclusions : Based on these results it can be assumed that ceftriaxone has a genetic potential. The frequency of micronuclei, nucleoplasmic bridges and nuclear buds in cytokinesis-blocked cultures of human peripheral blood lymphocytes increases with increasing concentrations of Ceftriaxone, with a significant difference in their frequencies relative to controls as was determined for each of the concentrations. Ceftriaxone present genotoxic, cytostatic and cytotoxic activity in the applied in vitro cytogenetic tests. Ceftriaxone in lymphocyte culture affects the inhibition of cells proliferation, as confirmed by the decrease of NDI (nuclear division index - indicator of cytostatic effect) and NDCI (nuclear division cytotoxicity index - indicator of cytotoxic effects) compared to the controls.

Objective: This study investigated the influence of sex and ageing on chromosomal damage and the role of life-style habits on the frequency of chromosomal aberrations (CAs) in peripheral blood lymphocytes (PBLs) of healthy Bosnian subjects. Materials and Methods: Peripheral blood samples were obtained from 100 healthy, unrelated individuals in Bosnia and Herzegovina during 2010 and 2011. Chromosome preparations were made by dropping and air drying and slides were stained with 10% Giemsa solution (pH 6.8). The cytogenetic analysis was carried out in a cytogenetic laboratory in the Department of Biology of the Faculty of Science in Sarajevo. The category of total structural CAs was sub classified as chromosome-type aberrations (CSAs) and chromatid-type aberrations (CTAs) while the category of total numerical CAs was sub classified as aneuploid and polyploid mitoses. All statistical analyses were carried out using Microsoft Excel 2010 (Microsoft Corporation) and the Windows Kwikstat Winks SDA 7.0.2 statistical software package (Texa Soft Cedar Hill, Texas). Results: Cytogenetic analysis revealed the average number of structural CAs was 2.84 and of numerical CAs was 9.56. There was a significant increase in the frequency of chromosome-type aberrations (1.92) compared with chromatid-type aberrations (CTAs) (0.92) and a significant increase in the frequency of aneuploid (8.83) compared with polyploid (0.73) mitoses. Significant positive correlations between age and CTAs in human PBLs were also demonstrated. Additional statistical analysis showed that ageing increase number of numerical CAs in lymphocytes of drinkers. The frequency of structural CAs of females exposed to radiation was significantly greater than in males. Analysis indicates the presence of a positive association between CAs and smoking in younger subjects but a negative correlation between aberrant cells frequencies and alcohol in older drinkers. Conclusion: The results of the study support the conclusion that sex and age, together with life-style habits of individuals as confounding factors can affect spontaneous frequency of CAs especially to CTAs in PBLs that are a biomarker of cancer risk.

Introduction: This study was performed to establish a baseline value of micronucleus frequency in buccal cells and to estimate the impact of the most common factors (sex and age, and smoking) on micronucleus and degenerative nuclear alteration frequencies in the sample of healthy Bosnian subjects.Methods: The Buccal Micronucleus Cytome (BMCyt) assay, based on scoring not only micronucleus frequency but also other genome damage markers, dead or degenerated cells, provides a measure of cytotoxic and genotoxic effects.Results: Our results showed the baseline buccal micronucleus frequency was 0.135% or 1.35‰, as well as positive correlations between micronucleus frequencies and formations of degenerative nuclear alterations (nuclear buds, karyolytic and karyorrhectic cells). The number of micronuclei in buccal cells was significantly higher in females than in males. There was positive association between the age and frequency of analysed cytogenetic biomarkers. Buccal cell micronuclei and degenerative nuclear alternations were more frequent among cigarette smokers than non-smokers and significantly higher in female smokers than in male smokers. Cytogenetic damages showed significantly positive correlation between intensity of smoking and the number of nuclear alterations. The years of smoking had a significant influence not only on the number of nuclear alterations but also in micronuclei and nuclear buds in buccal cells.Conclusions: The sex influences the number of micronuclei in human buccal cells. The ageing increased the number of micronuclei and other biomarkers of DNA damage. The cigarette smoking significantly increases the frequencies of micronuclei and nuclear buds, pyknotic, karyolytic and karyorrhectic cells.

ABSTRACT Introduction: Alprazolam is a triazolobenzodiazepine used in panic disorders and other anxiety states. Target organ of Alprazolam is CNS, causing depression of respiration and consciousness. Aim: This study aimed to estimate the genotoxic potential of Alprazolam using Allium cepa test. Methods: Allium cepa is one of the most suitable plants for detecting different types of xenobiotics. The test enables the assessment of different genetic endpoints making possible damage to the DNA of humans to be predicted. Results: Alprazolam induced chromosomal (anaphase bridges, breaks, lagging and stickiness, abnormal spiralisation, multipolarity and polyploidy) and cytological aberrations, especially nuclear alterations (nuclear buds, fragmented nucleus and apoptotic bodies, cells without nucleus, binucleated and micronucleated cells), morphological alterations in shape and size of cells, spindle disturbance and polar deviation in root tip meristem cells of Allium cepa at all tested concentrations. Alprazolam also caused significant inhibition of mitotic index in these cells. Conclusion: These changes in cells are indicators of genotoxic potential of Alprazolam suggesting a need for further in vitro studies on animal and human lymphocytes as well as in vivo studies.

Objective: In recent years regional anesthesia has gained great popularity. However, like any other medical procedure, the regional anesthesia carries certain risk of unintended intraneural injection and consequential neurological complications. Studies in animals have suggested that intraneural application of local anesthetics may cause mechanical injury. Previous studies, however, have used small animal models and clinically irrelevant injection speed or equipment. In this study we used equipment and injection methods in common clinical use to study the consequences and pressure dynamics of intraneural injection. Our hypothesis is that an intraneural injection is heralded by higher injection pressure and leads to neurologic impairment in pigs. Materials and Methods: Ten pigs of mixed breed (21-26 kg, 4-6 months old) were studied. After general anesthesia, the sciatic nerves (n = 20) were exposed bilaterally. Under direct vision, a 25-gauge insulated nerve block needle was placed either intraneurally (n = 10) or perineurally (n = 10), and 4 ml of preservative-free lidocaine 2% was injected using an automated infusion pump (15 ml/min). Injection pressure data were acquired using an in-line manometer coupled to a computer via an analog-to-digital conversion board. After injection, the animals were awakened and subjected to serial neurologic examinations during next 7 days. Results: All perineural injections resulted in injection pressures below 40 kPa. In contrast, intraneural injections resulted in significantly higher peak pressures (P 140 k Pa. Conclusion: High injection pressure (>140 kPa) predicts intraneural injection and consequential neurologic deficit. As long as the injection pressure is low, injection into poorly compliant tissue can be avoided and neurological complication can be prevented.

Introduction: Allium cepa is a biological material of great importance for mutagenicity tests, due to the simplicity of its use in testing, the fast growth of its roots, and the response of genetic material to the presence of substances with cytotoxic and genotoxic potential. Method: The Allium test model by using the onion seed (Alium cepa L.) without the use of pesticides or any other chemicals is used in this research. Progressive changes in Allium cepa’s chromosomes caused by treating Allium cepa’s roots in selected drug concentration of 1, 10, 50 and 100 μg/ml of Alprazolam were followed. For statistical analysis chi-square test with the level of significance set at P≤0.05 is used. Results: Changes in the structure and number of chromosomes in mitosis were found. Conclusion: Comparing the results obtained for genotoxicity of Alprazolam with those obtained in the experiments that have been performed on other preparations of this group of drugs it can be stated that Alprazolam has similar effects on the cell cycle. Because of potential risk of using Alprazolam another experiment with higher concentration of Alprazolam would give more sufficient information.