Plant oils have attracted interest for centuries as natural remedies in treatment of various diseases. The Inula verbascifolia (Willd.) Hausskn.isgrowing wild plant in Bosnia and Herzegovina. Aromatic natural oils are one of the most significant sources of natural organic components. The natural vegetable oil of the selected plant (Inula verb.) was obtained by the hydrodistillation method. A comparison was made between the fragrant vegetable oil from the aerial parts of the plant in the flowering period (summer) and after the flowering period (autumn). In this study, chemical compounds were tested, comparing the content and composition of natural oils from the plant Inula verbascifolia. The aerial parts of the plant contained a fragrant and yellow essential oil. The identified 125 constituents accounted for 86.87% and 88.38% of the oil. The dominant compounds of both EOs were tridecanal, (3Z)- hexenyl benzoate, α-murolol, hexadecanoic acid, linalool and undecanal. Since essential aromatic oils possess a number of antimicrobial properties, an analysis of antimicrobial activity was also performed in this work. The antimicrobial activity of a mixture of EOs was determined on selected ATCC strains of microorganisms. Results of antimicrobial activity indicated that all used the microorganisms were sensitive to the EO. No data about antimicrobial activity of Inula verbascifolia has been published yet.

Copper (II) complexes with commercial antibiotics, amoxicillin (AMX), azithromycin (AZT) and ciprofloxacin (CFL) were synthesized and isolated as solids. Structures of the isolated products were determined by FTIR spectroscopy. Antibacterial activities were determined on reference bacterial strains from the ATCC collection by diffusion technique. The results show that AMX and CFL coordinate Cu (II) ion as bidentate O-donor ligand. AZT coordinates metal center as bidentate NO-donor ligand. A difference in the morphology of antibiotic crystals and the synthesized complexes was found. Complex of Cu (AMX)2 show complete absence of antibacterial activity, while the other com-plexes show the same or even lower activity than the parent ligands.  

Aims: The aims of this study was to investigate the susceptibility of extended-spectrum beta-lactamase (ESBL) producing Klebsiella pneumoniae clinical isolates to antibiotics and essential oils - Origanum compactum, Origanum majorana and Thymus serpyllum. Study Design: Study included 30 isolates of Klebsiella pneumoniae obtained from clinical material provided from the University Clinical Center Tuzla. Place and Duration of Study: Department of Biology, Faculty of Science, University of Tuzla, BiH, between September 2019 to September 2020. Methodology: Antibiotic susceptibility testing was performed by the Kirby-Bauer disk diffusion method. The following commercially available antibiotic discs were used: amoxicillin (30µg), cefalexin (30 µg), gentamicin (10 µg), amikacin (30 µg), imipenem (10 µg), piperacillin (75µg), ampicilin (10 µg), meropenem (10 µg), ciprofloksacin (10 µg), ceftazidim (30 µg), cefotaksim (30 µg), ceftriaxone (30 µg), cefepime (30 µg) and aztreonam (30 µg). The antibacterial effect of the essential oils was tested for ESBL K. pneumoniae isolates using the diffusion method according to Clinical laboratory standards institute (CLSI) guidelines. Results: O. compactum and O. majorana essential oils showed the same antimicrobial activity with 80.0% effect on ESBL K. pneumoniae isolates, Thymus serpyllum EO showed antimicrobial activity of 60.0%. The lowest MIC value had the O. compactum essential oil (MIC 6 mg/ml-10.5 mg/ml), followed by the T. serpyllum (MIC 17.2 mg/ml-43 mg/ml), while the O. majorana essential oil showed MIC values in range from 11 mg/ml to 39 mg/ml. Conclusion: The results of the study showed the exceptional sensitivity of ESBL K. pneumoniae clinical isolates to the essential oils from Origanum and Thymus genera, which highly suggests their potential application in the struggle against these pathogens in the future.

In this study, metal complex of Copper(II) with a Schiff base derived from 2,2-dihydroxyindane-1,3-dione and 2-aminoethanoic acid were synthesized. The product are characterized by spectral methods. The antimicrobial activity was tested on reference bacterial strains and the antioxidant capacity was analyzed by using the DPPH and FRAP methods. The spectral data indicates that the Schiff base coordinates the Copper(II) as a tridentate ONO donor ligand. The compounds showed weaker antimicrobial activity on certain tested microorganisms. In vitro testing of antioxidant activity showed a significant reducing ability of the complex, as well as inhibitory activity against DPPH radicals.

People's interest in the safe use of food has existed since ancient times, so that even today there is a global demand for the production of food that can be used without risk to health. Contaminants, as well as the type and location of grazing can affect the physico-chemical and sensory properties of honey. The aim of the study was to examine the microbiological validity and sensory properties of honey from two different areas in Tuzla Canton. The research included three samples of forest honey from the Kladanj area and three samples of flower honey from the Lukavac area. In addition to microbiological analyzes and sensory properties, the concentration of HMF in honey was determined as an indicator of heating and inappropriate storage of honey. All samples were microbiologically correct. The sample of flower honey L1, which did not meet the criteria prescribed by the Regulation on Honey and Other Bee Products, was not sensory evaluated. L1 sample was not sensory evaluated due to an increased HMF content of 105.38 mg/kg. Based on sensory analysis, the samples of flower honey from Lukavac were better evaluated than forest honey from Kladanj. Statistical analysis showed that there is a significant difference between the mean values for the sensory properties of purity and color. Keywordshoney, healt safety, HMF, sensory analysis.