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Enida Karić

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Capsella bursa-pastoris (L.) Medik. (known as shepherd's purse) is a plant whose parts are used as medicine in herbal medicine. It is applicable as a medicine in the treatment of all forms of internal bleeding, for the treatment of hemorrhoids, excessive menstruation, but also for the usual stopping of nosebleeds. Through this research, the influence of organic solvents and their aqueous mixtures on the efficiency of polyphenol extraction and antioxidant activity was compared. The inhibition of free radicals was tested by the DPPH method, while the FRAP method was used to test the reduction potential. Analyzes have shown that water is the most effective solvent in the isolation of polyphenols from the aerial parts of shepherd's purse. Mixtures of organic solvents with water also showed high efficiency in the extraction of bioactive components, while the weakest results were obtained for extracts prepared in pure organic solvents.

Enida Karić, E. Horozić, S. Pilipović, E. Dautović, M. Ibišević, Amra Džambić, Semir Čeliković, Arnela Halilčević

Extracts obtained from plant material have widely applied in the chemical and pharmaceutical industries because they contain significant concentrations of biologically active substances. Commercial daisy extract (Bellis perennis) was used in this paper for in vitro testing of tyrosinase enzyme inhibition, and antioxidant and antimicrobial activity. Inhibition of the tyrosinase enzyme was determined by monitoring dopachrome formation at a wavelength of 492 nm. Antioxidant activity was tested using FRAP and DPPH methods, while antibacterial activity was tested by diffusion technique on reference strains from the ATCC collection. The results showed that daisy extract inhibits tyrosinase enzyme in a dose-dependent manner. The extract effectively neutralized DPPH radicals and also showed good reducing ability. Bacterial strains used for in vitro antimicrobial activity testing did not show sensitivity to the extract concentrations used in this study.

Origanum compactum, an endemic Moroccan medicinal herb, possesses many different activities such as antibacterial, antifungal, antioxidant and anticancer. The aim of this study was to investigate the stability and antifungal activity of liposomal dispersion with this essential oil. Liposomal dispersion stability was evaluated by testing the vesicle size, polydispersity index and zeta potential. It was also examined the in vitro release of thymol and carvacrol from liposomal dispersion. The major components of this essential oil were carvacrol (58.4%), thymol (12.5%) and γ-terpinene (10.7%). Origanum compactum essential oil showed a strong antifungal activity, and the inhibition zones ranged from 24 to 45 mm. After 210 minutes, 80.88% thymol and 16.67% carvacrol were released. Stability assessment was performed for three months and the liposomal dispersion showed a good stability.

In this study, metal complex of Copper(II) with a Schiff base derived from 2,2-dihydroxyindane-1,3-dione and 2-aminoethanoic acid were synthesized. The product are characterized by spectral methods. The antimicrobial activity was tested on reference bacterial strains and the antioxidant capacity was analyzed by using the DPPH and FRAP methods. The spectral data indicates that the Schiff base coordinates the Copper(II) as a tridentate ONO donor ligand. The compounds showed weaker antimicrobial activity on certain tested microorganisms. In vitro testing of antioxidant activity showed a significant reducing ability of the complex, as well as inhibitory activity against DPPH radicals.

A. Jukić, E. Vidović, F. Faraguna, Lucija Rebrović, Mihovil Medić, Đ. Ačkar, Veronika Barišić, Ivana Flanjak, J. Babić et al.

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