Extracts obtained from plant material have widely applied in the chemical and pharmaceutical industries because they contain significant concentrations of biologically active substances. Commercial daisy extract (Bellis perennis) was used in this paper for in vitro testing of tyrosinase enzyme inhibition, and antioxidant and antimicrobial activity. Inhibition of the tyrosinase enzyme was determined by monitoring dopachrome formation at a wavelength of 492 nm. Antioxidant activity was tested using FRAP and DPPH methods, while antibacterial activity was tested by diffusion technique on reference strains from the ATCC collection. The results showed that daisy extract inhibits tyrosinase enzyme in a dose-dependent manner. The extract effectively neutralized DPPH radicals and also showed good reducing ability. Bacterial strains used for in vitro antimicrobial activity testing did not show sensitivity to the extract concentrations used in this study.

Origanum compactum, an endemic Moroccan medicinal herb, possesses many different activities such as antibacterial, antifungal, antioxidant and anticancer. The aim of this study was to investigate the stability and antifungal activity of liposomal dispersion with this essential oil. Liposomal dispersion stability was evaluated by testing the vesicle size, polydispersity index and zeta potential. It was also examined the in vitro release of thymol and carvacrol from liposomal dispersion. The major components of this essential oil were carvacrol (58.4%), thymol (12.5%) and γ-terpinene (10.7%). Origanum compactum essential oil showed a strong antifungal activity, and the inhibition zones ranged from 24 to 45 mm. After 210 minutes, 80.88% thymol and 16.67% carvacrol were released. Stability assessment was performed for three months and the liposomal dispersion showed a good stability.

 merima.ibisevic@untz.ba ABSTRACT: Origanum compactum (Lamiaceae) is an endemic species of oregano from Morocco, and the main components are carvacol and thymol, which are considered to have antimicrobial activity. Essential oils can be unstable, poorly soluble in water and poorly delivered to target cells. The incorporation of essential oils into liposomes can reduce their irritant effect, while at the same time prolonging the action of the preparation itself as well as increasing its effectiveness. The aim of our study was to investigate antimicrobial activity of liposomal and non-liposomal vaginal suppositories, and see if there are any differences in antimicrobial activity. Examination of the antimicrobial activity of vaginal suppositories was examined in the same way as the antimicrobial activity of the essential oil, by the disk diffusion method. There were used standard bacterial strains from ATCC collection: Staphylococcus aureus ( S. aureus ) ATCC 25923, Enterococcus faecalis ( E. faecalis ) ATCC 51299, Escherichia coli ( E. coli ) ATCC 25922, Candida albicans ( C. albicans ) ATCC 10231. Liposomal vaginal suppositories had a smaller inhibition zones probably due to the slower release of active components, but still have an advantage over non-liposomal vaginal suppositories because they reduce the irritating potential of the

The funerary stela of Marcus Aurelius Bitus, a soldier of the Legio IV Flavia Felix, was discovered in Palmotićeva Street, Belgrade, during construction work undertaken in 1989-1990. The stela is typologically classified as being of the architectural type, and is topped by a pediment decorated with lions. To date, it remains the only known example of an intact stela with a pediment not only in Singidunum, but also along the entire Upper Moesian Limes. This paper provides evidence of the fact that this type of stelawith-pediment was not uncommon, either within Singidunum itself or throughout its wider surroundings. Additionally, the structural and iconographic features of the stela of Marcus Aurelius Bitus are studied, and the issue of artistic influences from Pannonia and Noricum, which undoubtedly influenced the stela's creation in a local workshop in what was then Singidunum, are also considered