The emergence of bacteria with antibiotic resistance and multiple resistance is characteristic of animal and human pathogens. It is wide known that bee products, which have been used in alternative medicine since ancient times, have antimicrobial potential. Application of bee products for therapeutic purposes is defined as apitherapy. The study aimed to evaluate the antimicrobial activity of commercial chestnut honey, pollen and propolis produced in western Bosnia and Herzegovina (Sanski Most) individually and in five combinations (apimixtures). The antimicrobial properties of samples were investigated using the agar well diffusion method against three Gram-positive bacteria (Bacillus subtilis subsp. spizizenii ATCC 6633, Methicillin-resistant Staphylococcus aureus ATCC 33591, Enterococcus faecalis ATCC 29212); three Gram-negative bacteria (ESBL producing Escherichia coli ATCC 35218, Salmonella enterica subsp. enterica serovar Enteritidis ATCC 13076, Pseudomonas aeruginosa ATCC 9027) and one fungal species (Candida albicans ATCC 10231). Pure bee pollen inhibited the growth of only Gram-negative bacteria, concentrated chestnut honey was active against all Gram-negative and Gram-positivebacteria, while 20% propolis extract and apimixtures A2 (80% honey and 20% propolis) and A3 (60% honey, 20% pollen and 20% propolis extract) inhibited the growth of all tested microorganisms. Chestnut honey andthree apimixtures (A1, A2 and A3) showed the highest antibacterial action against all tested Gram-negative bacteria and MRSA compared to other investigated samples. In this study, examined honeybee products from Bosnia and Herzegovina and their mixtures had significant activity against tested bacteria, including strains with proven resistance to conventional antibiotics, MRSA and ESBL producing E. coli.

Background: The Harderian gland in domestic birds is a major paraocular excretory gland that has an important role in tear production as well as in the immune protection of the conjunctiva surface. Aim: The aim of this research was to investigate the arterial and venous supply of the gland in hens and provide valuable and useful information for future research. Methods: The research was conducted on 26 adult hens, provenience of Lohmann Brown. For the identification and determination of blood vessels, we used the vascular corrosion cast technique in conjunction with the transmission electron microscope (TEM). Results: The casts showed that the gland receives the arterial supply via branches of a. ophthalmotemporalis and a. nasalis communis and these arteries are accompanied by the corresponding veins. Ultrastructural analyses showed the presence of fenestrated capillaries, which indicates the possibility for permeability of larger molecules. Conclusion: The present research gives important and detailed information about the arterial and venous supply of the Harderian gland in hens that may serve as guidelines for future vascular and morphological investigations.

The use of digital teaching resources became widespread and very helpful during the COVID‐19 pandemic as an alternative to a traditional course with cadavers. Technologies such as augmented reality (AR), virtual reality (VR), 3D models, video lectures and other online resources enable three‐dimensional visualization of the anatomical structures and allow students to learn more interactively. The aim of this study was to compare students' performance in the traditional anatomical courses in teaching neuroanatomy and technology‐based learning methods such as video lectures, 3D models and 3D printed specimens. Four groups of first‐year students of Veterinary Faculty established for the practical classes during the academic year 2021/2022 took part in this research. The total number of students participating in this research was 72. Each group attended separately the theoretical lecture with a demonstration based on a different technique; the control group used formalized specimens, while the three experimental groups used video lectures, 3D models and 3D printed specimens, respectively. Subsequently, all groups completed the same questionnaire testing their short‐term memory of the neuroanatomical structures. After four weeks students were tested for their long‐term memory of the neuroanatomy lecture with the follow‐up test containing an identical list of questions. The test scores using video lectures and 3D printed models were significantly higher compared with the group that learned in the traditional way. This study suggests that alternative approaches such as technology‐based digital methods can facilitate memorization of anatomical terms and structures in a more interactive and sensory engaging way of learning.

Abstract Lesser mole-rats (Nannospalax leucodon) are members of the Rodentia order’s Spalacidae family, and they are found in Northeastern Africa, the Balkans, Southeastern Europe, Central Asia, the Middle East, and Caucasia. The shape of the skull has a significant impact on the phenotypic appearance of animal heads, and although many domestic species have been studied, there is a lack of evidence on the macro-anatomical characteristics of the skeletal system in mole-rats. The current research was focused on the morphological, morphometric, and radiographic properties of lesser mole-rats skull in Bosnia and Herzegovina. The research was conducted on five lesser mole-rats from Bjelasnica Mountain, Bosnia and Herzegovina. We compared the results of the previously published studies, and we found a lot of similarities between Nannospalax leucodon in Bosnia and Herzegovina and Nannospalax ehrenbergi in North Iraq, as well as the Nannospalax nehringi from Eastern Anatolia.

Dairy cattle breeding is one of the most important branches of livestock production, which has been facing, for several decades, the chronic problem of declining reproductive performance. In 2005, the number of cattle worldwide was about 1,370,000,000, while in 2015 that number dropped below one billion, and in 2021 it shows a slight recovery as it was 1,000,970. This indicates the importance of applying different reproductive protocols in order to increase the number of cows in production. The type of bedding on which the animals stay, as well as the characteristics of the lying area itself, shows a significant impact on numerous physiological functions such as food intake, chewing, milk yield, but also levels of sex hormones. The type of bedding and lying area, which causes chronic pain and stress, leads to disorders of physiological and reproductive processes, since stress has direct negative impact on numerous cellular functions. A total of 66 dairy cows, 50 Holstein-Friesian cows kept on PD Butmir and 16 Simmental cows kept on a private mini farm, were included in the study. At PD Butmir, cows were kept in tie-stall housing system, while on a mini-farm they were kept free. Hormonal protocols of estrus and ovulation synchronization were used in April, May and June 2019. Cows were subjected to two estrus synchronization and ovulation protocols, Ovsynch and Cosynch72. At PD Butmir, 25 cows were subjected to Ovsynch and Cosynch72 protocols, respectively. At the mini-farm only Ovsynch protocol was applied. The Ovsynch protocol applied on PD Butmir had success in conception rate of 12% (n = 3), while the Cosynch72 protocol gave a score of 36% (n = 9). On the mini-farm, Ovsynch resulted in a conception of 25% (n = 4). Based on our results, the Cosynch72 protocol, compared to the Ovsynch protocol, was a better choice in the case of Holstein-Friesian cows kept in the tie-stall housing system. In the Simmental cows kept in the free stall system, the Ovsynch protocol proved to be better choice in achieving conception, compared to the Holstein-Friesian cows. Therefore, it is necessary to test several different protocols of estrus and ovulation synchronization, in order to find the most optimal one for a certain breed, type of keeping and breeding.

There is no doubt that milk and dairy products have a high nutritional value. However, their current consumption is increasingly dependent on their dietary and health properties. It is noticeable that in recent years, increasing popularity is given to those dairy products that have a positive effect on the human body. The aim of this study was to determine the fatty acid composition of sheep's milk from the area of Livno and Travnik (Vlašić), with special reference to the content of bioactive fatty acids that have a positive effect on human health. The fatty acid composition of the tested milk samples was specific due to the content of fatty acids which have been proven to have an extremely beneficial effect on human health. Concentrations of most bioactive fatty acids varied between areas, and the differences were statistically significant for arachidonic, eicosapentaenoic, docosahexaenoic, and rumenic. Milk samples from the tested areas had an almost ideal ratio of n-6/n-3 fatty acids, which makes them very favorable foods from a health point of view.

Microvascularization of domestic fowl kidneys was studied using scanning electron microscopy (SEM) of vascular corrosion casts (VCCs). Two types of nephrons, mammalian‐type (MT) and reptilian‐type (RT) nephrons and their glomerular structure were analysed quantitatively by 3D morphometry. A significant difference in shape and size between the MT and RT glomeruli was found. The mean diameter of the RT glomeruli was about 56 µm, while that of MT glomeruli was significantly larger, namely about 80 µm. The afferent arterioles in mammalian‐type glomeruli usually bifurcated into two lobular branches and formed a complex glomerular capillary network with numerous loops. Reptilian‐type glomeruli consisted of a single capillary forming few loops and leaving the glomerulus as efferent arteriole. Diameters of afferent and efferent arteriolar replicas were similar in all three kidney divisions of MT and RT nephrons. The absence of the interconnecting branches between the MT nephron capillaries at the gross inspection suggests that the mammalian‐type nephron glomeruli, although more complex than the reptilian type, are not equivalent to those in mammalian kidneys.

This paper presents a comprehensive anatomic overview of normal pulmonary vascularization in an American mink (Neovison vison), with emphasis on venous and arterial peculiarities as regards its course and potential disruptions. The study is designed as macro-morphological and vascular network analysis of lungs of fifteen minks of different age and gender. Dissection is conducted along with injection corrosion method in order to clearly visualize the vascular topology, bronchial tree, arterial and venous trees, and their interconnections. The specimens exhibit consistence in the arterial branching pattern with negligible range of alterations. It was noticed that upon leaving the right ventricle of heart, pulmonary trunk divides ventrally from the site of tracheal bifurcation. The divisions were identified as left and right pulmonary arteries, which then ramified in the left and right lung, respectively. Left a. pulmonalis further divides into two major branches ramus lobi cranialis and ramus lobi caudalis, while the right a. pulmonalis gives five major branches for lobes in the right lung. Total of five pulmonary veins leave pulmonary lobes and enter left atrium of the heart. Mink has a distinct niche in biomedical research, proving as a suitable model to enhance the understandings of the various diseases. Gained insights are valuable as reference values for examination of the vasculature in other Mustelidae species, recognition of potential deviations and vascular remodeling due to respiratory diseases.

Abstract The study has aimed to investigate and determine the anatomical position, shape, size, and histological features of the ductus venosus, and its role as a shunt in the fetal circulatory system in domestic ruminants. The research was conducted on 19 bovine, 11 sheep and 5 goat fetuses, aborted at the late stage of pregnancy or deceased just after delivery. The general anatomy of the ductus venosus was investigated by in-situ dissection of the corrosive cast obtained by injection of 25% solution of Vinylite mass through the umbilical vein. For histological examination, the fetal tissue samples were stained with Hematoxylin and Eosin, Masson’s trichrome, Verhoeff-Van Gieson and Gomoriꞌs silver stain. The results showed that ruminant fetal ductus venosus is a curved, trumpet-shaped vessel, situated in the central part of the liver, above the porta hepatis. Its ventral part is constricted in the form of an isthmus, having a prominent lip-like thickening at the junction with the portal sinus. Histological examination showed the dominant presence of collagen and elastic fibers in its tunica media, with thin bands of smooth muscle fibers oriented in a longitudinal and circular direction indicating ability for vasoconstriction and vasodilatation.

| The aim of this study was to morphologically describe the air sac system in Rosy-faced parrots (Agapornis roseicollis) and provide useful data for future veterinary care and treatment of respiratory system diseases in these pets. The research was conducted on five birds. In order to obtain the casts of the air sacs, we applied the corrosion cast technique, where we used the 26% solution of Vinylite mass. The research showed that the anatomy of the air sacs in these parrots was very similar to that in other birds, but there was some specific characteristic regarding the arrangement and connection between the air sacs. In Rosy-faced parrots we identified nine air sacs, the one unpaired, saccus clavicularis and paired saccus cervicalis, thoracicus cranialis, thoracicus caudalis and abdominalis. The casts showed that clavicular and cranial thoracic air sacs established a connection through the diverticula sternalia. These anatomical characteristics can be common to birds from Psittaciformes order and additional research need to be performed to confirm these findings.

3D printing or additive manufacturing is one of the newest technologies that has begun to be used for production of anatomical models in veterinary medicine. The main aim of this research was to evaluate the total cost required for 3D printing of different anatomical models and the cost-effectiveness of use of these technologies in practice. For purpose of this study, we evaluated a cost of 3D printed model of the human heart, inner ear, cross-section of the animal cell and spinal cord, sheep, cow and cat brain and complete dog model with internal organs. The costs were calculated separately for raw material consumption and personnel operative work. The results show that 3D printing technologies are cost-effective for the production of anatomical models, and the total cost depends mostly on working time of the personnel.

that the air sacs. The morphology of the air sacs system has been described in many domestic and wild bird Abstract | The research was conducted with the aim to investigate the morphology of air sacs system in Crimson Rosella ( Platycercus elegans ) parrots. Five adult birds, two males and three females were used in this study. The lungs and air sac system were injected via trachea with 26% solution of Vinylite mass. The obtained cast showed that these parrots have nine air sacs. The clavicular air sac was the only unpaired, while the cervical, cranial thoracic, caudal thoracic and abdominal air sacs were paired. The morphology of the air sacs was generally similar to that reported in other bird species, however, some specific features were identified. As most prominent among them were a partial fusion of the cervical air sacs, communication between the left and right subpectoral and perirenal diverticula and connection between the claviclar and cranial thoracic air sacs. The present investigation provided detailed and comprehensive data about the morphology of air sacs system in these parrot species and these findings will be very useful for future clinical examination and treatment of this birds.

Leptospirosis is an acute, subacute and chronical contagious disease of animals and humans. Causative agents of this disease belong to the genus Leptospira, family Leptospiraca. As a disease of wild animals, leptospirosis is widespread through Europe. Certain wild animals (rodents, fox and wild boars) are important reservoirs and highly probable vectors for the spread of infection into domestic animals and humans. During the hunting season, hunting dogs are often in direct or indirect contact with wild animals that could be carriers of this disease, and the possibility of appearance and spreading within this cohort of dogs is very high. The main reasons for this study on the prevalence of Leptospirosis in Bosnia and Herzegovina are the regular contact between hunting dogs and wild animals (carriers), and the lack of dataaboutLeptospirosisinhuntingdogs.In total, 175 serum samples from 15 towns of Bosnia and Herzegovina were tested. Twelve serovars of L. interrogans were used in the microscopic agglutination test. Presence of antibodies of four serovars was confirmed. Prevalence of seropositive dogs was 15.4% (27/175). Most positive dogs had a reaction to the Pomona serovar 51.8% (n=14), while the prevalence of the Sejroe serovar was 33.3%, Icterohaemorrhagiae serovar 11.1% and Bratislava serovar 3.7%. The highest number of positive reactions 55.5% (n=15) was with serum dilution of 1:200. This study showed that most infections in dogs were caused by serovars that are currently not included in commercial vaccines. One of the most efficient preventive measure could be vaccination with the serovars most often found in wild animals, as they appear to be the most common source of the infection.