Artemisinin and its derivatives kill malaria parasites and inhibit the proliferation of cancer cells. In both processes, heme was shown to play a key role in artemisinin bioactivation. We found that artemisinin and clinical artemisinin derivatives are able to compensate for a mutation in the yeast Bcs1 protein, a key chaperon involved in biogenesis of the mitochondrial respiratory complex III. The equivalent Bcs1 variant causes an encephalopathy in human by affecting complex III assembly. We show that artemisinin derivatives decrease the content of mitochondrial cytochromes and disturb the maturation of the complex III cytochrome c1. This last effect is likely responsible for the compensation by decreasing the detrimental over-accumulation of the inactive pre-complex III observed in the bcs1 mutant. We further show that a fluorescent dihydroartemisinin probe rapidly accumulates in the mitochondrial network and targets cytochromes c and c1 in yeast, human cells and isolated mitochondria. In vitro this probe interacts with purified cytochrome c only under reducing conditions and we detected cytochrome c-dihydroartemisinin covalent adducts by mass spectrometry analyses. We propose that reduced mitochondrial c-type cytochromes act as both targets and mediators of artemisinin bioactivation in yeast and human cells.

Background: Necrotizing enterocolitis (NEC) is the most common life-threatening gastrointestinal emergency associated with prematurity. Timely diagnosis and adequate treatment are crucial to reduce the morbidity and mortality of the affected infants. The aim of this study was to evaluate the diagnostic yield of bowel dilatation on plane abdominal radiography (AR) in the early diagnosis and NEC severity in preterm infants. Methods: We retrospectively reviewed initial ARs of 50 preterm infants with NEC ≥ stage II admitted to the neonatal intensive care unit (NICU) in a tertiary-care hospital. The largest bowel loops diameters (AD), the latero-lateral diameters of the peduncle of the first lumbar vertebra (L1), and the distance of the upper edge of the first lumbar vertebra and the lower edge of the second one, including the disc space (L1–L2), were measured. All anteroposterior ARs were done in a supine projection on the day of onset of the initial symptoms of NEC. Results: Preterm infants with surgical NEC showed a statistically significant increase in the AD/L1 ratio (p < 0.001) and AD/L1-L2 ratio (p < 0.001) compared with preterm infants with medical NEC. We found no significant association between the site of the most distended bowel loop and the severity of NEC (p > 0.05). Conclusion: Bowel loop distension on initial AR may serve as an additional diagnostic tool in the early diagnosis and severity of stages II/III NEC. Further prospective clinical studies should validate the results from this study.

Nonorthogonal multiple access (NOMA) significantly improves the connectivity opportunities and enhances the spectrum efficiency (SE) in the fifth generation and beyond (B5G) wireless communications. Meanwhile, emerging B5G services demand for higher SE in the NOMA-based wireless communications. However, traditional ground-to-ground (G2G) communications are hard to satisfy these demands, especially for the cellular uplinks. To solve these challenges, this article proposes a multiple unmanned-aerial-vehicles (UAVs)-aided uplink NOMA method. In detail, multiple hovering UAVs relay data for a half of ground users (GUs) and share the spectrums with the other GUs that communicate with the base station (BS) directly. Furthermore, this article proposes a K-means clustering-based UAV deployment scheme and location-based user pairing (UP) scheme to optimize the transceiver association for the multiple UAVs-aided NOMA uplinks. Finally, a sum power minimization-based resource allocation problem is formulated with the lowest Quality-of-Service (QoS) constraints. We solve it with the message-passing algorithm and evaluate the superior performances of the proposed scheduling and paring schemes on SE and energy efficiency (EE). Extensive simulations are conducted to compare the performances of the proposed schemes with those of the single UAV-aided NOMA uplinks, G2G-based NOMA uplinks, and the proposed multiple UAVs-aided uplinks with a facility location framework-based UAV deployment. Simulation results demonstrate that the proposed multiple UAVs deployment and UP-based NOMA scheme significantly improves the EE and the SE of the cellular uplinks at the cost of only a little relaying power consumption of UAVs.

The paper proposes a problem-solving approach in the area of underground mining, related to the evaluation and selection of the optimal mining method, employing fuzzy multiple-criteria optimization. The application of fuzzy logic to decision-making in multiple-criteria optimization is particularly useful in cases where not enough information is available about a given system, and where expert knowledge and experience are an important aspect. With a straightforward objective, multiple-criteria decision-making is used to rank various mining methods relative to a set of criteria and to select the optimal solution. The considered mining methods represent possible alternatives. In addition, various criteria and subcriteria that influence the selection of the best available solution are defined and analyzed. The final decision concerning the selection of the optimal mining method is made based on mathematical optimization calculations. The paper demonstrates the proposed approach as applied in a case study.

This paper integrates a different perspective into the diaspora literature, by placing it within the frame of digital diasporas and war time engagement in actions and initiatives traditionally considered as diplomatic. We reconstruct how digital diaspora diplomacy developed during a time when the Internet was relatively new and diplomatic tools were limited due to an ongoing conflict in Bosnia and Herzegovina. We examine BOSNET, an online epistemic community of Bosnian diaspora IT pioneers, with a shared set of normative and principled set of beliefs about the independence of their homeland, and collected, shared and spread information about what was going on in their country. We label their work as ‘policy innovation’ engagement and performativity as 'informal' behaviour, as it was unscripted, uncoded and unregulated by any written conventions or state strategies.

We show that scattering from the boundary of static, higher-order topological insulators (HOTIs) can be used to simulate the behavior of (time-periodic) Floquet topological insulators. We consider D-dimensional HOTIs with gapless corner states which are weakly probed by external waves in a scattering setup. We find that the unitary reflection matrix describing back-scattering from the boundary of the HOTI is topologically equivalent to a (D-1)-dimensional nontrivial Floquet operator. To characterize the topology of the reflection matrix, we introduce the concept of `nested' scattering matrices. Our results provide a route to engineer topological Floquet systems in the lab without the need for external driving. As benefit, the topological system does not to suffer from decoherence and heating.

Abstract The goal of this study was to determine the content of 12 metals in 23 samples of teeth from two cities in Bosnia and Herzegovina (B&H): Sarajevo, a capital city with heavy traffic, industrial facilities, and long periods of smog during winter and Bihac, a picturesque small city, with no industrialization, settled among vivid national park and rivers. The teeth were separated into enamel and dentin. Dissolution of samples was performed in concentrated HNO3 with the addition of H2O2 followed by flame atomic absorption spectroscopy (FAAS) analysis. The results showed expected high contents of Ca, Na, Mg, and K, while elevated contents of Cu, Fe, and Zn were present in some samples. K and Na showed uniform distributions throughout enamel and dentin. Alkaline and earth-alkaline metals showed significant positive correlations. Zinc and manganese exhibited differences in the dentin content based on the place of residence. Zinc also displayed statistically significant differences between smokers’ and nonsmokers’ dentin samples. The differences were more pronounced between intra groups (within one sample) than for inter groups (within different groups, such as location, gender, and smoking).

Background White spot lesions (WSLs) are a common complication after orthodontic treatment. The aim of this study was to characterize and compare the antimicrobial properties of selenium-containing vs. fluoride-containing orthodontic materials. Material/Methods Antibacterial efficacy of orthodontic materials (SeLECT Defense bonding agent, Adhesive agent, Band Cement, Transbond Plus SEP bonding agent, Transbond Plus Adhesive agent, Fuji I Band cement, Fuji Ortho LC Adhesive agent, Ortho Solo Bonding agent, Transbond XT bonding agent, and Transbond XT primer) was tested with the inhibition of 2 bacterial strains: S. mutans (ATCC 10449) and L. acidophilus (ATCC 4356). The antimicrobial efficacy of the materials was measured by agar diffusion test. The diameters of inhibition zones around each disk were measured in millimeters (mm). Results Materials containing selenium and fluoride showed significant differences from the negative control (both p<0.001). Orthodontic materials containing fluoride as a potential antimicrobial agent showed larger zones of inhibition in total (9.1±2.6 mm), the selenium group was the second-most effective (4.7±4.9 mm), and the group without any potential antimicrobial agent showed the least antimicrobial effect (0.9±1.0 mm). Materials from the group with no antibacterial agent were not significantly different from the negative control group (p>0.05). Conclusions Materials containing selenium carried the most significance when comparing microorganisms with the agent, since they were the only ones showing difference between the 2 microorganisms. They showed statistically significant difference in efficacy against S. mutans, and poor antimicrobial effect against L. acidophilus. These data suggest that orthodontic materials containing selenium might have the potential to prevent WSLs due to their antimicrobial properties.