This research presents the first findings on thrombopoiesis for Wistar rats. Haemopoietic cells from the femur and the sternum were analysed by light microscopy in combination with infrared and near-ultraviolet light for fine cytoplasmic structure analysis. Five main types of thrombocyte precursor cells were identified in the bone marrow samples: megakaryoblast, promegakaryocyte and megakaryocyte (basophilic, acidophilic and thrombocytogenic). More intensive thrombopoiesis and morphologically differentiated cells were found in sternum samples.

U ovom radu ponudit će se rodna analiza potreba i mogućih pravaca reforme izbornog zakonodavstva Bosne i Hercegovine. Fokus rada će biti na tri centralna istraživačka pitanja. Prvo, analizirat će se u kojoj mjeri i na koji način konsocijacijski politički sistemi o(ne)mogućavaju predstavljanje žena u politici. Pri tome će se u obzir uzeti različite dimenzije predstavljanja (formalno, deskriptivno, sadržajno i simbolično predstavljanje). Drugo, analizirat će se da li je Izborni zakon Bosne i Hercegovine usklađen sa Zakonom o ravnopravnosti spolova Bosne i Hercegovine. I treće, ispitivat će se u kojem pravcu se trebaju i mogu odvijati reforme izbornog zakonodavstva BiH. U tom kontekstu biće predstavljene i preporuke CEDAW komiteta i različite rodno odgovorne inicijative za reforme izbornog zakonodavstva koje dolaze kako od institucija nadležnih za rodnu ravnopravnost, tako i iz sfere civilnog društva.

MicroRNA-based medicines have drawn attention as a promising tool for the treatment of various diseases. Due to the poor biomembrane permeation, cellular uptake, and enzymatic instability of naked microRNA, the clinical success of gene therapy is mostly dependent on formulating efficient and safe transfection vectors [1]. Therefore, we aimed to develop cationic solid lipid nanoparticles (SLN) for gene therapy purposes. SLN containing 0.15% of stearylamine, 4.85% of Precirol ATO 5 (solid lipid), 1% of Tween 80, and 1% of Poloxamer 188, as non-ionic surfactants, were produced using a high-pressure homogenization process (800 bar and three cycles). microRNA 27-a was further complexed with SLN in the following SLN/microRNA ratios: 1:5, 1:2.5, 1:1, 2.5:1, 5:1, 10:1, 25:1 and characterized using dynamic light scattering, electrophoretic light scattering, and gel retardation assay. The SLN had a mean diameter of 112 ± 0.5 nm (PDI of 0.202 ± 0.011) and a zeta potential (ZP) value of +30.6 ± 1.25 mV. Complexation of SLNs with microRNA decreased a particle size from 244.8 ± 2.7 to 120.4 ± 0.4 nm with an increasing weight ratio of SLNs, while the biggest particle size was observed in 1:1 ratio (1146 ± 110.2 nm) due to low ZP values (3.45 ± 0.2 mV). Further, ZP increased from -14.3 ± 0.4 mV to + 39.7 ± 0.5 mV. Both ELS data and gel retardation assay results revealed that complete complexation could be attained above the weight ratio of 5:1. Our investigations suggest that SLN poses a high potential to be non-viral gene carriers in miRNA replacement therapy.

......................................................................................................................... 6 Introduction .................................................................................................................... 7 Theory ............................................................................................................................ 9 Computational Details and Methodology .......................................................................... 13 Results and Discussion Gibbs Reaction Energies ...................................................................................... 16 Dipoles and First Hyperpolarizabilities ................................................................... 22 Gibbs Activation Energies and Thermal Half-Lives ................................................. 25 Wiberg Indices .................................................................................................... 29 Conclusions .................................................................................................................. 33 References .................................................................................................................... 34

In this paper, the tradeoff between spectrum efficiency (SE) and energy efficiency (EE) is investigated in terms of interference management and power allocation for heterogeneous networks (HetNets) with non-orthogonal multiple access (NOMA). The EE and SE tradeoff is modeled as a multi-objective problem (MOP) under the maximum power and quality of service (QoS) constraints, which is non-convex. The MOP is relaxed into a convex single objective problem (SOP) by adopting a weighted sum strategy with the hypograph transformation. The SOP is solved in two steps. In the first step, we propose a power allocation technique based on non-cooperative (NC) game for EE and SE in NOMA HetNets. In the proposed NC game, the macro base station (MBS) and the small BSs (SBSs) compete with an equal priority in order to optimize their transmit powers towards maximizing the weighted sum of SE and EE. In the second step, a closed-form formula is proposed to control the power allocated to users taking into account both QoS constraint and successive interference cancellation (SIC) condition. From simulations, the proposed technique can, in some dedicated settings, considerably improve the tradeoff between EE and SE over conventional techniques.