Hurricane Ida ferociously affected many south-eastern and eastern parts of the United States, making it one of the strongest hurricanes in recent years. Advanced forecast and warning tool has been used to track the path of the ex-Hurricane, Ida, as it left New Orleans on its way towards the northeast, accurately predicting significant supercell development above New York City on September 01, 2021. This advanced method accurately detected the area with the highest possible level of convective instability with 24-h lead time and even Level 5, devised in the categorical outlooks legend of the system. Therefore, an extreme level implied a very high probability of the local-scale hazard occurring above the NYC. Cloud model output fields (updrafts and downdrafts, wind shear, near-surface convergence, the vertical component of relative vorticity) show the rapid development of a strong supercell storm with rotating updrafts and a mesocyclone. The characteristic hook-shaped echo signature visible in the reflectivity patterns indicates a signal for a highly precipitable (HP) supercell with the possibility of tornado initiation. Open boundary conditions represent a good basis for simulating a tornado that evolved from a supercell storm, initialized with initial data obtained from a real-time simulation in the period when the bow echo and tornado-like signature occurred. Тhe modeled results agree well with the observations.

Apoptosis induction is a promising approach in targeting tumor cells. As halogenated boroxine (HB) shows antitumor activity, but its mechanism of action in hematological tumors remains unclear, in this study, we aimed to analyze apoptosis triggering in normal and UT‐7 leukemia cells by HB. Methods for assessing cell viability and cytotoxicity, apoptosis detection, relative expression of 84 apoptosis‐associated genes and BCL‐2, and functional analysis were applied. Pronounced HB activities in inhibition of cell viability, cytotoxicity, and apoptosis induction with measurable differences between tumor and normal cells were found. HB modulated the expression of 21 genes, predominantly downregulated the antiapoptotic genes in leukemia. The functional association revealed HB's impact on inhibition of NF‐κB signaling pathway. BCL‐2 expression decreasing was found only in UT‐7 leukemia. This study identified HB as an apoptosis inducer affecting leukemia but not normal cells considering mechanisms of selective activity that may be a great advantage of HB applications.

Drying of textiles in industrial facilities represents an energy-intensive process where a large number of measures for energy and production cost savings can be introduced. Typical measures include the introduction of energy management, waste-heat recovery, process optimization and so on. Drying is a complex process with coupled heat and mass transfer between the heated air and humid textile, where parameters such as the air flow rate, air velocity and its flow regime and textile velocity and water content represent significant influential factors. The distribution of air temperature and density inside the drying section of an industrial stenter frame is analyzed in detail using three-dimensional numerical simulation, where the textile is modeled as a porous medium to analyze moisture diffusion within the textile. Heated air is introduced into a chamber by inlet nozzles and removed by exit nozzles, the distribution of which is based on actual machine configuration. A humid textile is introduced into a section, where temperature and density distribution within the textile are calculated for selected time periods. During the simulation in the Fluent program, models of specific component transport, multiphase air flow, turbulent flow, porosity and evaporation were used. The results represent a valuable data set that provides an in-depth insight into the drying process in the industrial stenter machine.

During the execution of construction projects, uncertain events, such as delays, prolongations and disruptions of project activities, have the potential to cause a significant deviation between the planned and realized state of a project. As a result, progress on important project objectives can decrease and this leads to critical delays as well as heavy profit loss. For this reason, we propose the implementation of the customized evolutionary algorithm to generate resilient baseline schedules which include a sufficient number of time floats to absorb the negative impact of uncertainty. This way, the baseline solution is searched as a trade-off between project duration, its final profit and the overall baseline stability. The proposed algorithm is applied to real construction project data and the results of the analysis suggest improved stability for resilient baseline schedules. Application of the genetic algorithm to solve the existing multi-objective problem enables practical implementation of new technologies and methods in construction management. Resilient baseline schedules can be used in an uncertain environment to achieve more accurate predictions and support decision making in the areas of construction scheduling and costing.

Healthcare workers (HCW) in primary healthcare centres in the Republic of Srpska, Bosnia and Herzegovina, are on the first combat line with COVID-19. This study aimed to assess the seroprevalence of SARS-CoV-2 among HCW at the primary healthcare centres and to analyse the risk exposure to COVID-19, clinical signs and vaccination status. A cross-sectional study was conducted among HCW at the selected primary healthcare centres between 19 March and 30 April 2021. Antibodies against the SARS-CoV-2 virus were detected by enzyme-linked immunosorbent assay (ELISA). A total of 1,023 HCW (mean age 45 years; 71% female) were included in the study. The anti-SARS-CoV-2 antibodies were detected in 69.5% of all participants. There was a significant difference in seropositivity among primary healthcare centres from different geographical regions. As many as 432 (42%) of all participants had confirmed COVID-19 symptoms before the study and, 84.8% of them were seropositive. This study showed that 702 primary HCW were vaccinated with any of these vaccines: Sputnik V, Sinopharm, Pfizer/Biontech. High titre of SARS-CoV-2 antibodies was found amongst those who received one (92.6%) or both (97.2%) doses of vaccines. In this study, we report high prevalence of SARS-CoV-2 antibody among HCW in primary healthcare in the Republic of Srpska, Bosnia and Herzegovina during the third pandemic wave.

Providing the population with sufficient quantities of hygienically healthy food is the foundation of public health protection. According to the current BiH Law on Food, the main goal is to ensure a high level of human health protection, and the measures applied should be based on risk assessment. Regular food monitoring is one of the key public health problems, and this paper presents the quantitative risk of copper intake by consuming mint herbal tea infusions. The average copper concentration in the 20 analyzed samples of mint herbal tea infusions was 0.0028 ± 0.0125 mg / kg, while the value of the average daily intake was less than the oral reference dose for copper, so that the hazard coefficient itself was <1 , and consuming the analyzed infusions of herbal mint tea has no harmful effects on the health of the surveyed children.

The modern concept of lightweight design (LW) requires the application of different materials in one structure (multi-material structures). The structure of different materials has a good perspective for application in the automotive and aerospace industries but only if it is possible to achieve a quality joint between different materials. The most used technology for joining different materials in the automotive industry is Resistance spot welding (RSW). Due to different mechanical, physical, and chemical properties, the joining of different materials by RSW technology does not provide a quality joint, and accordingly, alternative technologies for joining different materials have emerged. Resistance element welding (REW) was developed to enable joint of different materials. This paper presents the welding of AW 5754 H22 Al alloy (1.0 mm-thick) and DP500 steel (1.5 mm-thick) using novel REW. The peak load, absorption energy, microstructure, microhardness and fatigue strength of the REW joint has been investigated. The joint of the same materials has been done also using conventional RSW to compare some results. The results that will be presented in this paper show that that REW can achieve reliable joining of the two materials at relatively low welding currents compared to RSW. Using REW process with a significantly lower welding current, satisfactory mechanical characteristics of the weld joint can be achieved, so peak load is between 2300–2500 N, displacement is between 2.5–3 mm and the absorption energy is between 3.3–5.7 J. REW joints showed fatigue strength with the fatigue limit of 882 N.

ABSTRACT Due to its economic worth, cotton (Gossypium hirsutum L.) is grown in almost 70 countries and provides income for more than 250 million people. Therefore, producing cotton with having some desired characteristics that includes extended biotic and abiotic stress tolerance, improved fiber quality, promoted nutritional content and increased yield is the main objective for cotton biotechnology. To achieve this goal, many tissue culture and gene transfer techniques are being developed and used throughout the years. As applications for the gene transfer, the Agrobacterium-mediated, particle bombardment and pollen tube pathway-mediated methods are most successfully used and in conjunction with this, meristematic shoot tips as explants are efficiently utilized in gene transfer methods. In this study, the main objective was to report an efficient protocol for a greater recovery of transgenic cotton plant using Agrobacterium tumefaciens-mediated transformation. For this, one of the cotton strains (Cukurova 1518) cultivated widely in Turkey was chosen and meristematic shoot tips as explant sources, and GFP and NPTII genes as reporter and marker genes were used, respectively. The effective post co-cultivation conditions were provided via using the selection regime in vitro. Finally, the current results showed highly reproducible protocol developed could be used to produce transgenic cotton plants expressing desired traits or can be utilized as a model system to study the expression of particular genes.

Abstract Background We compared the accuracy of preoperative transvaginal ultrasound (TVUS) versus magnetic resonance imaging (MRI) for the assessment of myometrial invasion (MI) in patients with endometrial cancer (EC), while definitive histopathological diagnosis served as a reference method. Patients and methods Study performed at a single tertiary centre from 2019 to 2021, included women with a histopathological proven EC, hospitalized for scheduled surgery. TVUS and MRI were performed prior to surgical staging for assessment MI, which was estimated using two objective TVUS methods (Gordon’s and Karlsson’s) and MRI. Patients were divided into two groups, after surgery and histopathological assessment of MI: superficial (≤ 50%) and deep (> 50%). Results Sixty patients were eligible for the study. According to the reference method, there were 34 (56.7%) cases in the study with MI < 50%, and 26 (43.3%) with MI > 50%. Both objective TVUS methods and MRI showed no statistical significant differences in overall diagnostic performance for the preoperative assessment of MI. The concordance coefficient between both TVUS methods, MRI and histopathology was statistically significant (p < 0.001). Gordon’s method calculating MI reached a positive predictive value (PPV) of 83%, negative predictive value (NPV) of 83%, 77% sensitivity, 88% specificity, and 83% overall accuracy. Karlsson’s method reached PPV of 82%, NPV of 79%, 69% sensitivity, 88% specificity, and 80% overall accuracy. Accordingly, MRI calculating MI reached PPV of 83%, NPV of 97%, 97% sensitivity, 85% specificity, and 90% overall accuracy. Conclusions We found that objective TVUS assessment of myometrial invasion was performed with a diagnostic accuracy comparable to that of MRI in women with endometrial cancer.