This article investigates the relationship between the dimensions of the Theory of Planned Behaviour (which includes attitude toward behavior, subjective norm, and perceived control behavior) and entrepreneurial intentions and intrapreneurial intentions, considering entrepreneurial orientation as a moderator. Using the snowball sampling method, cross-sectional data were collected from 437 respondents. After testing for reliability and validity using confirmatory factor analysis, hypotheses were tested using hierarchical regression. The results indicate that the attitudes toward entrepreneurship and perceived behavioral control positively relate to entrepreneurial intentions. In contrast, attitude toward intrapreneurship is the only dimension of the theory of planned behavior positively related to intrapreneurial intentions. The moderating role of entrepreneurial orientation is only significant for the relationship between attitude toward entrepreneurship and entrepreneurial intentions. Based on the results, several recommendations are made for businesses and policymakers to boost entrepreneurial activity among the current labor force.

Objetivo: Apresentar a implantação e desenvolvimento de um processo sistematizado de prescrição de Projeto Terapêutico Singular (PTS) para pacientes internados em um Centro de Reabilitação do Centro-Oeste brasileiro e seus desdobramentos e potencialidades para aplicação em diversos perfis de pacientes hospitalizados. Relato de experiência: A metodologia de prescrição e gerenciamento de PTS nos serviços da instituição conta com o envolvimento do paciente, sua família e equipe multiprofissional, que desempenham diversos papéis durante o processo: desde a avaliação de diagnóstico e prognóstico, até o gerenciamento de dados e desenvolvimento de planos de ação de melhoria. A implantação do PTS conforme a metodologia proposta se mostrou adaptável e funcional para gerenciar a jornada do paciente e auxiliou a organização e manejo da equipe diante dos prognósticos apresentados. A prescrição do PTS trouxe mais agilidade e funcionalidade na avaliação e manejo dos casos, proporcionando condutas mais assertivas voltadas às necessidades e desejos dos pacientes. Considerações finais: O gerenciamento do PTS contribui para o alcance de diversas metas institucionais e proporciona melhores desfechos clínicos aos pacientes, impactando diretamente na qualidade de vida dos mesmos, além da redução de custos e melhoria da performance da gestão sócio-econômica da saúde pública.

In Herzegovina table grapes have been grown on smaller private areas. One of the most important factors that affect the yield and quality of vines, grapes and the profit of production are the viruses. The aim of this work is to monitor the occurrence of 4 viruses (GLRaV-1, GLRaV-3, ArMV and GFLV) on 6 table grape varieties: Prima, Black Magic, Cardinal, Demir-kapija, Victoria, Alphonse Lavallée. The research was conducted in the period from November 2019 to September 2020 on one of the larger plantations north of Mostar.Studies have shown different susceptibility of different varieties to these viruses. Of the 60 samples examined, the presence of ArMV virus was not recorded. The presence of GLRaV-1, GLRaV-3 and GFLV viruses was proven by ELISA. The variety Victoria is most susceptible to infection with the GLRaV-3 virus, in which each sample of this variety is infected. The second variety that is most susceptible is Demir kapija with 80% of infected samples, followed by Cardinal with 70% and Alfonso with 50%. The most resistant varieties according to this research are Prima with 30% of infected samples and Black Magic with 10% of infected samples.

Juvenile systemic sclerosis (jSSc) is an orphan disease with a prevalence of 3 in 1 000 000 children. In adult patients there are significant differences between the clinical presentation of diffuse and limited subtypes. We reviewed clinical differences in presentation of subtypes in patients in the juvenile systemic scleroderma inception cohort (jSScC).To study the clinical presentation of jSSc patients with diffuse (djSSc) and limited (ljSSc) subtypes.We reviewed the baseline clinical characteristics of the patients, who were recruited to the jSScC till December 2022. jSScC is a prospective cohort of jSSc patients, who developed the first non-Raynaud´s symptom before the age of 16 years and are under the age of 18 years at the time of inclusion.The JSScC included 232 patients, 68% (n=159) had diffuse subtype. The median age at onset of Raynaud phenomenon was 10.4 years (7.3-12.9), at the first non-Raynaud symptom 10.9 years (7.3-13.0) and median disease duration 2.5 years (1.0-4.6). The female/male ratio was significantly lower in the djSSc subtype (3:1 versus 5:1, p<0.001). Antibody profile was similar, with the exception of a significantly higher number of anticentromere positive patients in the ljSSc. Decreased FVC<80% was found in approximately 30% and decreased DLCO<80% was found in around 40% in both subtypes. Abnormal HRCT findings were found in 44% of patients. Pulmonary hypertension assessed by ultrasound occurred in approximately 5% in both groups and gastrointestinal involvement in 43% of djSSc and 36% in ljSSc (p=0.303). Patients with djSSc had significantly higher modified Rodnan Skin Score, more frequently sclerodactyly, a history of digital ulceration active ulceration, telangiectasia, a decreased Body Mass Index z score ≤ -2 and decreased joint range of motion. Patients with ljSSc had significantly higher rate of cardiac involvement. Regarding patient related outcomes assessed by VAS 0-100 djSSc patients had more severe disease also physician related outcome assessed by VAS 0-100 were significantly higher in djSSc (see Table 1).Table 1.Comparison of subtypes at time of inclusion in the cohortWhole Group N=232Diffuse Subtype N=159Limited Subtype N=73P value Anticentromere5% (7/156)2% (2/106)10% (5/50)0.022 MRSS, median (IQR)10 (4 – 20)16 (8 - 27)4 (0 – 8)0.001 Gottron Papules26% (59/228)31% (48/155)15% (11/73)0.011 Sclerodactyly75% (165/219)85% (127/150)55% (38/69)<0.001 Telangiectasia37% (77/209)44%(62/141)22% (15/68)0.002 History of ulceration52% (119/229)62% (98/158)30% (21/71)<0.001 Active ulceration17% (39/229)21% (33/158)8% (6/71)0.021 Only Cardiac involvement5% (12/232)3% (4/159)11% (8/73)0.007 BMI<- 2 z score15% (33/217)20% (29/148)6% (4/69)0.008 Joints with decreased range59% (136/231)64% (101/158)48% (35/73)0.022Physician Reported (Median, IQR) Physician global disease activity30 (20 – 45)n=19735 (20– 50)n=13820 (10 – 30)n=590.001 Physician global disease damage30 (15 – 40)n=19530 (20 – 45)n=13820 (5 – 30)n=570.004 Physician ulceration activity0 (0 – 16)n=2165 (0 – 20)n=1540 (0 – 0)n=620.018Patient Reported (Median, IQR) Patient global disease activity40 (20 – 50)n=17840 (20 – 50)n=12930 (15 – 55)n=490.024 Patient global disease damage30 (15 – 60)n=17740 (20 – 60)n=12825 (5 – 55)n=490.001 Patient Raynaud activity30 (10 – 60)n=20230 (10 – 60)n=14515 (0 – 55)n=570.001 Patient ulceration activity0 (0 – 30)n=20310 (0 – 30)n=1450 (0 – 20)n=580.001In the largest jSSc cohort in the world, djSSc patients have a significantly more severe disease. Patients and physician related outcomes were significantly more severe in djSSc group. Interestingly, we found no differences regarding interstitial lung disease, pulmonary hypertension or gastrointestinal involvement, although the number of patients with decreased BMI ≤ -2 z score was significantly higher in the djSSc patients.NIL.NIL.None Declared.

Children infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) usually present minimal symptoms or are asymptomatic. Nevertheless, a subset of children 2-6 weeks after the initial SARS-CoV-2 infection develops a postinfectious SARS-CoV-2-related multisystem inflammatory syndrome in (MIS-C). Recently, transient expansion of TRBV11-2 T cell clonotypes in MIS-C was associated with signatures of inflammation and T cell activation, however, the underlying pathophysiology of MIS-C is not fully understood [1].The purpose of our project is to characterize the complexity of cell populations and capture cellular heterogeneity to uncover the regulatory networks and interactions that are disrupted during MIS-C flare with simultaneous profiling of gene expression and open chromatin regions from the same nuclei.Samples of peripheral blood mononuclear cells from patients with MIS-C diagnosed at the University Children’s Hospital, University Medical Center Ljubljana, were collected during the initial presentation before any treatment and at 6-12 months in remission. The primary aim is to identify which regulatory networks are driving inflammation in MIS-C flare, for which we are performing single cell Multiome ATAC + Gene Expression Sequencing. To enable simultaneous profiling of epigenomic landscape and gene expression from the same nuclei, we are using Chromium Next GEM Single Cell Multiome ATAC + Gene Expression kit from 10X Genomics.We included 32 patients with MIS-C from whom we collected paired blood samples during the initial presentation before treatment and at 6-12 months in remission. In single cell multiomic experiment we included 10 patients with paired samples, with the most viable cell count prior cryopreservation. All samples that are included into multiomic single cell analysis have 75% - 99% viability prior cryopreservation. In the protocol the key is to remove remaining granulocytes causing high mitochondrial RNA burden and extensively optimize the dilution factor of lysis buffer and the length of cell lysis step in order to get intact nuclei with no significant blebbing. Afterward, the single cell ATAC libraries as well as single-cell gene expression libraries are constructed and sequenced. Data are undergoing pairwise analysis to compare the cell population heterogeneity, expression profile and open chromatin landscape in the time of the initial presentation of MIS-C and in the remission, with Cell ranger software as well as with R package scREG [2], and custom scripting. In the second step we will inspect if the resulting altered transcriptomic signature from single-cell experiment is present on larger cohort. In that regard, we will perform bulk transcriptomic profiling on all paired collected samples during the initial presentation of MIS-C before treatment and at 6-12 months in remission.The results of this project are expected to enlighten the underlying pathophysiology of MIS-C flare and thus support clinical decision on more targeted treatment. The identified disrupted networks during MIS-C flare could lead the way to establish an early diagnosis and improve long-term outcome, including prevention of myocardial and neuropsychological impairment. Moreover, a better understanding of the disrupted regulatory networks that are driving inflammation in MIS-C, could lead to new insights into diseases with similar clinical presentations as is Kawasaki Disease.[1]Sacco, K., Castagnoli, R., Vakkilainen, S.et al.Immunopathological signatures in multisystem inflammatory syndrome in children and pediatric COVID-19.Nat Med28, 1050–1062 (2022).[2]Duren, Z., Chang, F., Naqing, F.et al.Regulatory analysis of single cell multiome gene expression and chromatin accessibility data with scREG.Genome Biol23, 114 (2022).This research was supported by Slovenian research agency grant J3-3061 and Interreg ITA-SLO project Cattedra.None Declared.

Obesity, a highly prevalent disorder and central diagnosis of the metabolic syndrome, is linked to mental health by clinical observations and biological pathways. Patients with a diagnosis of obesity may show long-lasting increases in risk for receiving psychiatric co-diagnoses. Austrian national registry data of inpatient services from 1997 to 2014 were analyzed to detect associations between a hospital diagnosis of obesity (ICD-10: E66) and disorders grouped by level-3 ICD-10 codes. Data were stratified by age decades and associations between each pair of diagnoses were computed with the Cochran-Mantel-Haenszel method, providing odds ratios (OR) and p values corrected for multiple testing. Further, directions of the associations were assessed by calculating time-order-ratios. Receiving a diagnosis of obesity significantly increased the odds for a large spectrum of psychiatric disorders across all age groups, including depression, psychosis-spectrum, anxiety, eating and personality disorders (all p _corr < 0.01, all OR > 1.5). For all co-diagnoses except for psychosis-spectrum, obesity was significantly more often the diagnosis received first. Further, significant sex differences were found for most disorders, with women showing increased risk for all disorders except schizophrenia and nicotine addiction. In addition to the well-recognized role in promoting disorders related to the metabolic syndrome and severe cardiometabolic sequalae, obesity commonly precedes severe mental health disorders. Risk is most pronounced in young age groups and particularly increased in female patients. Consequently, thorough screening for mental health problems in patients with obesity is urgently called for to allow prevention and facilitate adequate treatment.

Epigenetic modifications play a vital role in the preservation of genome integrity and in the regulation of gene expression. DNA methylation, one of the key mechanisms of epigenetic control, impacts growth, development, stress response and adaptability of all organisms, including plants. The detection of DNA methylation marks is crucial for understanding the mechanisms underlying these processes and for developing strategies to improve productivity and stress resistance of crop plants. There are different methods for detecting plant DNA methylation, such as bisulfite sequencing, methylation-sensitive amplified polymorphism, genome-wide DNA methylation analysis, methylated DNA immunoprecipitation sequencing, reduced representation bisulfite sequencing, MS and immuno-based techniques. These profiling approaches vary in many aspects, including DNA input, resolution, genomic region coverage, and bioinformatics analysis. Selecting an appropriate methylation screening approach requires an understanding of all these techniques. This review provides an overview of DNA methylation profiling methods in crop plants, along with comparisons of the efficacy of these techniques between model and crop plants. The strengths and limitations of each methodological approach are outlined, and the importance of considering both technical and biological factors are highlighted. Additionally, methods for modulating DNA methylation in model and crop species are presented. Overall, this review will assist scientists in making informed decisions when selecting an appropriate DNA methylation profiling method.

The aim of this paper is to predict the positions of the first just noticeable difference (JND) points as optimal levels of compression for images and videos based on simple features derived from the original visual signals. Three image datasets and one video dataset with subjectively defined JNDs were used in the analysis. We show that the position of the first JND point can be successfully predicted on the basis of simple image features. The highest degree of agreement with subjectively assigned JNDs was reached by features based on the gradient magnitude, where on two datasets with JPEG images their correlation is greater than 95%. On the dataset with VVC images, which has a larger number of images and a wider range of image content, the degree of agreement between gradient-based predictions and the results of subjective tests is 84%, while the correlation on the dataset with video sequences is 80%.

This paper provides a comprehensive study on the learning models' power violation, sum-rate performance while taking into consideration power constraint, and computational efficiency in terms of training and execution times over a dynamic wireless channel. We propose a reward shaping method and modify learning models with the output scaling strategy to enforce them to fully respect the power constraints while optimizing the sum-rate performance. The proposed approach reaches close-to-optimal accuracy, i.e., up to 99.15%, while satisfying the predefined power constraint of the base station. Moreover, learning models are shown to be more computationally efficient compared to the traditional algorithm. However, solving the power allocation problem within the Orthogonal Frequency Division Multiplexing (OFDM) symbol duration of $16.7\mu \mathrm{s}$ is a remaining challenge.

This paper presents a comprehensive study on the efficiency and effectiveness of exploration policies for deep reinforcement (DRL) algorithms with applications to the power allocation problem in multi-carrier wireless systems. We propose three distinct exploration functions, i.e., linear, fast and slow, to balance exploration and exploitation in the dynamic wireless environment. We analyze the effect of exploration on the initial training length as well as learning models' sum-rate performance and power violation probabilities. Our results indicate that the DRL algorithms with the proposed exploration functions reach close-to-optimal sum-rate performance within only 1000 training episodes (i.e., equivalent to 8.01 s) while satisfying the predefined power constraint of the base station.

Objectives: vasculitis is a rare disease characterized by inflammation and necrosis of blood vessels. inflammation-induced thrombosis is a hallmark of several autoimmune diseases, such as systemic lupus erythematosus (sle), rheumatoid arthritis (ra), sjögren’s syndrome (ss)

This study aimed to compare sprint skating profile characteristics of the different playing positions of junior and senior bandy players. In total, 111 male national-level bandy players (age: 20.7 ± 5.0 years, height: 1.80 ± 0.05 m, body mass: 76.4 ± 0.4 kg, training experience: 13.8 ± 5.0 yrs) were tested on their sprint skating profile over 80 m. The main findings were that no differences between positions were found in sprint skating performance (speed and acceleration), but that elite players were in general heavier (p < 0.05) than junior players (80.0 ± 7.1 vs. 73.1 ± 8.1 kg), they could accelerate faster (2.96 ± 0.22 vs. 2.81 ± 0.28 m/s2), and they reached a higher velocity (10.83 ± 0.37 vs. 10.24 ± 0.42 m/s) earlier over 80 m than the junior players. This implies that junior level players should spend more time in power and sprint training to meet the specific demands of playing at a higher, elite level.