COVID-19 pandemic, caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), was declared in 2020 by the World Health Organization. New mutations have been identified, leading to various variants of this virus, including Alpha, Beta, Gamma, Delta, and Omicron, which are classified as variants of concern (VOCs) and have raised considerable concerns for global public health. Such constant spread and changes in the genome of the virus require continuous monitoring. This research focuses on the evolution of SARS-CoV-2 through a detailed presentation of the viral genome, protein structure and interpretation, with the presentation of phylogenetic characteristics and patterns. We obtained the sequence data from the European region focusing on the S, E, and RdRp proteins from the publicly available NCBI database. We next used the MEGA11 package to generate the multiple sequence alignments and create phylogenetic trees. The SWISS-MODEL server was connected to the Protein Data Bank to obtained tertiary structure images of all the proteins presented in the paper. Stability studies of obtained mutations were performed via MUpro online tool. The results indicate a substantial impact of the Omicron variant relative to others, particularly concerning the alterations and mutations observed in the spike (S) protein, which is crucial in the infection process.

Purpose. The aim of the study was to examine the effects of five weeks detraining period on body composition changes in elite professional Indonesian male soccer players. Material and methods. The body composition of 24 top-level male soccer players (age: 26.33 ± 4.06 years) competing in First League of Indonesia was measured before and after the 5-weeks detraining period. Body composition variables, including body mass, body fat percentage, fat-free mass, and total body water percentage, were measured using the bioelectrical impedance method. The results. The paired samples T-test showed significant changes in the means of body composition variables for body fat percentage, fat-free mass, and total body water percentage. Mean values for body fat percentage increased during the detraining period from 10.87 ± 2.47% to 11.98 ± 2.75% (10.3%). A decrease in mean values for fat-free mass (61.34 ± 5.74 kg to 59.80 ± 6.22; 2.49%) and total body water percentage (from 65.13 ± 1.84% to 64.40 ± 1.99%; 1.12%) was observed. No significant changes occurred in body mass (from 69.05 ± 8.07 kg to 69.05 ± 8.48 kg; 0.04%) from initial to final testing. Conclusions. Since there was a significant difference in body fat percentage, fat-free mass, and total body water percentage% before and after detraining period., it is suggested that 5 weeks of detraining period.  for the professional soccer players may not be necessary, and the duration should be shortened to maintain body composition status.

We introduce Mathador-LM, a new benchmark for evaluating the mathematical reasoning on large language models (LLMs), combining ruleset interpretation, planning, and problem-solving. This benchmark is inspired by the Mathador game, where the objective is to reach a target number using basic arithmetic operations on a given set of base numbers, following a simple set of rules. We show that, across leading LLMs, we obtain stable average performance while generating benchmark instances dynamically, following a target difficulty level. Thus, our benchmark alleviates concerns about test-set leakage into training data, an issue that often undermines popular benchmarks. Additionally, we conduct a comprehensive evaluation of both open and closed-source state-of-the-art LLMs on Mathador-LM. Our findings reveal that contemporary models struggle with Mathador-LM, scoring significantly lower than average 3rd graders. This stands in stark contrast to their strong performance on popular mathematical reasoning benchmarks. The implementation of Mathador-LM benchmark is available at https://github.com/IST-DASLab/Mathador-LM.

ABSTRACT Background In 2020, the coronavirus disease 2019 (COVID-19) pandemic caused disruptions in kidney replacement therapy (KRT) services worldwide. The aim of this study was to assess the effect of the COVID-19 pandemic in 2020 on the incidence of KRT, kidney transplantation activity, mortality and prevalence of KRT across Europe. Methods Patients receiving KRT were included from 17 countries providing data to the European Renal Association Registry. The epidemiology of KRT in 2020 was compared with average data from the period 2017–2019. Changes occurring during the first and second waves of the pandemic were also explored. Results The incidence of KRT was 6.2% lower in 2020 compared with 2017–2019, with the lowest point (−22.7%) during the first wave in April. The decrease varied across countries, was smaller in males (−5.2%) than in females (−8.2%) and was moderate for peritoneal dialysis (−3.7%) and haemodialysis (−5.4%) but substantial for pre-emptive kidney transplantation (−23.6%). The kidney transplantation rate decreased by 22.5%, reaching a nadir of −80.1% during the first wave, and was greatest for living donor kidney transplants (−30.5%). While in most countries the kidney transplantation rate decreased, in the Nordic/Baltic countries and Greece there was no clear decrease. In dialysis patients, mortality increased by 11.4% and was highest in those 65–74 years of age (16.1%), in those with diabetes as the primary renal disease (15.1%) and in those on haemodialysis (12.4%). In transplant recipients, the mortality was 25.8% higher, but there were no subgroups that stood out. In contrast to the rising prevalence of KRT observed over the past decades across Europe, the prevalence at the end of 2020 (N = 317 787) resembled that of 2019 (N = 317 077). Conclusion The COVID-19 pandemic has had a substantial impact on the incidence of KRT, kidney transplant activity, mortality of KRT and prevalence of KRT in Europe with variations across countries.

Abstract Objectives To develop two ethical checklists to evaluate (i) management of ethical concerns in research projects and (ii) awareness of ethical conduct of healthcare laboratory professionals. Methods Comprehensive discussion among the members of IFCC Task Force on Ethics based on pertinent literature. Results This Checklist for Clinical Research Projects should be useful to evaluate research proposals from an ethical perspective before submitting it to an IRB or its equivalent, thereby diminishing rejection rates and resulting in more time-effective projects. The checklist designed to evaluate the ethical conduct in laboratory medicine could be useful for self evaluation (internal audits) and for certification/accreditation processes performed by third parties. Conclusions These checklists are simple but powerful tools useful to guide professionals to adhere to ethical principles in their practice, especially in developing countries where accredited ethics committees may be difficult to find.

Purpose The present study aimed to investigate a) the associations between bilateral performance utilizing countermovement jump (CMJ), squat jump (SJ), speed and unilateral CMJ, isokinetic peak torque in knee extension and flexion with angular velocities of 60°/s and 180°/s and tensiomyography (TMG) parameters; b) whether the asymmetries derived from unilateral tests are associated with bilateral CMJ, SJ and speed in elite female soccer players. Methods Thirty-five elite female soccer players (average age: 20 ± 5 years) completed CMJ, SJ, speed, isokinetic muscle strength and TMG tests. Results Compared to the non-dominant leg, the dominant leg demonstrated greater peak torque output in both knee flexion (7.4%) and knee extension (5.6%) isokinetic tasks, as well as m. vastus medialis contraction time (7.6%), and soccer-specific agility test (4.1%). Conversely, the hamstring to quadriceps peak torque ratio at 180°/s (8.5%) was significantly greater in the non-dominant leg. The associations between CMJ, SJ and speed performance were positive and ranged from weak (r = 0.350) to high (r = 0.710). For speed and TMG-derived variables, correlations were negative and ranged from weak (r = −0.345, p = 0.042, for vastus medialis contraction time) to moderate (r = −0.530, p = 0.001, for biceps femoris contraction time). Furthermore, both bilateral CMJ and SJ negatively correlated with TMG-derived variables, ranging from weak (r = −0.350, p = 0.039, for vastus lateralis contraction time) to moderate (r = −0.537, p = 0.003, for rectus femoris contraction time). Conclusion The overall significant, albeit inconsistent, correlations between the diverse performance scores obtained highlight the necessity for a multifaceted and thorough diagnostic strategy in female soccer players.

The Internet of Things (IoT) is one of the key technologies for the digital transformation of both business and society. The paper primarily aims to investigate the structure and dynamics of academic publications in English dealing with issues of IoT development – from a marketing perspective, using methods of systematic mapping, i.e., co-citation analysis, bibliographic coupling, and analysis of the co-occurrence of keywords in the Scopus and Web of Science (WoS) databases. Analysis of research papers enables the identification of the most influential articles, papers, and journals, as well as visualization of the keywords and co-authorship co-occurrences. There has been an exponential increase in studies on IoT applications in marketing, indexed in Scopus and WoS citation databases. An insight into analyzed papers shows that companies utilize large amounts of data generated by the IoT to gain insights into customers’ supply and value creation, which helps strengthen their relationships with customers and pursue more efficient marketing policies and practices, ultimately leading to a competitive advantage. The IoT is expected to evolve into a vast network that includes smart devices and significantly influences people’s behavior, especially decision-making at different stages of the purchasing process.

Background Type 1 diabetes mellitus (T1DM) management in children and adolescents requires intensive supervision and monitoring to prevent acute and late diabetes complications and to improve quality of life. Digital health interventions, in particular diabetes mobile health apps (mHealth apps) can facilitate specialized T1DM care in this population. This study evaluated the initial usability of and satisfaction with the m-Health intervention Diabetes: M app, and the ease of use of various app features in supporting T1DM care in rural and remote areas of Bosnia-Herzegovina with limited access to specialized diabetes care. Methods This cross-sectional study, performed in February–March 2023, evaluated T1DM pediatric patients who used the Diabetes: M app in a 3-month mHealth-based T1DM management program, along with their parents and healthcare providers (HCPs). All participants completed self-administered online questionnaires at the end of the 3-month period. Data were analyzed by descriptive statistics. Results The study population included 50 T1DM patients (children/parents and adolescents) and nine HCPs. The mean ± SD age of the T1DM patients was 14 ± 4.54 years, with 26 (52%) being female. The mean ± SD age of the HCPs was 43.4 ± 7.76 years; all (100%) were women, with a mean ± SD professional experience of 17.8 ± 8.81 years. The app was reported usable in the domains of ease-of-use and satisfaction by the T1DM children/parents (5.82/7.0), T1DM adolescents/young adults (5.68/7.0), and HCPs (5.22/7.0). Various app features, as well as the overall app experience, were rated positively by the participants. Conclusion The results strongly support the usability of mHealth-based interventions in T1DM care, especially in overcoming care shortage and improving diabetes management and communications between HCPs and patients. Further studies are needed to compare the effectiveness of apps used to support T1DM management with routine care.

Introduction: Acute lymphoblastic leukemia (ALL) is the most frequent pediatric leukemia; it can be defined according to chromosomic and genomic data. Cytogenetic analyses and determination of chromosomal numbers (such as hypo- or hyperdiploidy) and/or specific chromosomal rearrangements are basic for ALL classification and treatment. Even though cure rates of childhood ALL are at ~95%, pharmacogenetic aspects are of raising importance. Material and Methods: We have analyzed the literature for ALL subtypes, corresponding therapy options, and pharmacogenetic implications. Results: Data for ALL subtypes such as B-ALL, T-ALL, Ph-like ALL, DS-ALL, ETP-ALL, BCR-ABL1-like ALL are presented here. The gene polymorphism which lead to metabolizability of 6-MP are ITPA variants (94C>A) and IVS2+21A>C, in conjunction with TPMT (238G>C, TPMT*3B 460G>A and *3C 719A>G and NUDT15 (415C>T). For methotrexate metabolism gene polymorphisms are found for gene MTHFR as C677T and A1298C. Conclusion: In the last decade in many hospital laboratories, pharmacogenetic aspects gain more and more importance. Application of many molecular biology methods provided progress in treatment and diagnosis of ALL patients. Combination therapy is proposed as an alternative to single drug treatments.

To investigate a robot’s role in children’s welfare and safety, we conducted interviews with 8 Subject Matter Experts and Professionals (SMEs) across the disciplines of robotics, child technology, psychology, and psychiatry disciplines. Through qualitative analysis, we synthesize the challenges of safeguarding, compounding limitations, and potential solutions for involving robots in safeguarding as broadly defined in SME interviews. While they agree robots should not be responsible for making judgement calls, the experts also identified the ways robots can be a valuable addition to the safeguarding team. However, more conversations spanning disciplines need to occur to inform policy and legal frameworks that will better establish a robot’s role in intimate spaces in children’s lives. While this line of inquiry is specific to robots in safeguarding, many of the themes reflect the nuances of finding appropriate places for child-robot interactions in the context of children’s welfare.

Background: Recent findings point to the key role of cathepsin S (CTSS) in the survival of malignant cells, as well as the significance of the anti-apoptotic properties of high-density lipoprotein (HDL) that contribute to enhanced cell survival. The purpose of this study is to analyze CTSS as a potential biomarker in lymphoma. Also, in order to better understand the role of CTSS in the origin and development of lymphoma, its association with cystatin C (Cys C), lipids, and inflammatory markers was analyzed.  Methods: The study included 90 subjects: 11 Hodgkin (HL) and 44 B-cell non-Hodgkin lymphoma (NHL) patients, and 35 healthy subjects. CTSS was determined using the Invitrogen ELISA kit (Thermo Fisher Scientific, Inc., Waltham, MA, USA). Results: The level of CTSS was significantly higher in NHL patients than in control subjects: 12.20 (9.75-14.57) vs 9.97 (8.44-10.99), P<0.001. In NHL patients, there was a positive correlation between CTSS and the proportions of HDL3a, HDL3b, and the sum of the HDL3 subclasses (r=0.506, P<0.001; r=0.411, P=0.006, r=0.335, P=0.026, respectively). In addition, the area under the receiver operating characteristic curve (AUC curve) of CTSS was 0.766 (CI: 0.655-0.856). Conclusions CTSS is significantly elevated in patients with NHL and has the potential to be a new diagnostic biomarker. Moreover, demonstrating a correlation between CTSS levels and the proportion of anti-apoptotic HDL3a and HDL3b subclasses improves understanding of NHL, as well as contributes to the development of new therapeutic strategies for this cancer.

Background Recent findings point to the key role of cathepsin S (CTSS) in the survival of malignant cells, as well as the significance of the anti-apoptotic properties of high-density lipoprotein (HDL) that contribute to enhanced cell survival. The purpose of this study is to analyse CTSS as a potential biomarker in lymphoma. Also, in order to better understand the role of CTSS in the origin and development of lymphoma, its association with cystatin C (Cys C), lipids, and inflammatory markers was analysed. Methods The study included 90 subjects: 11 Hodgkin (HL) and 44 B-cell non-Hodgkin lymphoma (NHL) patients, as well as 35 healthy subjects. CTSS was determined using the Invitrogen ELISA kit (Thermo Fisher Scientific, Inc., Waltham, MA, USA). The non-denaturing 3%-31% polyacrylamide gradient gel electrophoresis method was used to separate plasma HDL particles. Results The level of CTSS was significantly higher in NHL patients than in control subjects: 12.20 (9.75-14.57) vs 9.97 (8.44-10.99), P<0.001. In NHL patients, there was a positive correlation between CTSS and the proportions of HDL3a, HDL3b, and the sum of the HDL3 subclasses (r=0.506, P<0.001; r=0.411, P=0.006, r=0.335, P=0.026, respectively). In addition, the area under the receiver operating characteristic curve (AUC curve) of CTSS was 0.766 (CI: 0.655-0.856) for NHL patients. There was no significant difference in CTSS values between the control group and patients with HL, nor significant correlations between CTSS and HDL subclasses in the HL group. Conclusions CTSS is significantly elevated in patients with NHL and has the potential to be a new diagnostic bio - marker for the detection of NHL. Also, this study was the first to unveil the association between serum CTSS levels and the proportions of anti-apoptotic HDL3a and HDL3b subclasses in NHL patients.

Background Among many genes which have been analyzed to understand obesity and related metabolic traits among children and adolescents, not many studies are conducted on LGALS3 gene, especially in population of children. A positive correlation of circulating galectin 3 serum levels with impaired blood glucose, high blood pressure and higher values of serum lipids and was found in general population. The aim was to investigate possible association of rs4644 with body mass index, glycaemia, and lipid profile in Serbian adolescents. Methods The study included 72 boys and 79 girls, 14-15 years of age. Among boys 51 (67.1%) had normal values of BMI, 11 (14.5%) were overweight, and 14 (18.4%) were obese. Among girls, 53 (63.9%) had normal BMI, 16 (19.3%) were overweight, and 14 (16.9%) were obese. Diabetes type 1 or 2, genetic syndromes, generalized inflammation, cardiovascular and malignant diseases were criteria for exclusion. Genotyping was performed by Real time PCR.

Background: The COVID-19 pandemic had a catastrophic impact on the live music sector, putting the entire live entertainment industry into something nearing complete shutdown for more than two years. Purpose: The live music sector is still recovering from the impact of COVID-19. Even though most restrictions were lifted at the start of 2023, the industry is facing several challenges. These include rising costs and a saturated market after a long period of inactivity. There are also gaps in the workforce and some consumers are still hesitant to return to live events. Methods: To answer the research question “How did representatives of the music industry make a living during COVID-19”, we conducted two surveys: 1) A desk survey where we analyzed the measures taken by the national government. 2) An online survey where we collected data directly from representatives of the music industry. Results: In Slovenia, measures were taken to alleviate the fall in revenues. In Bosnia and Herzegovina, they were left to fend for themselves. Conclusions: Music events are an essential part of the local economy. They bring in tourists, create jobs, and generate income for local businesses. Live music events also provide a platform for musicians to showcase their talent and build a fan base.