Sleep deprivation is a significant contributor to various diseases, leading to poor cognitive function, decreased performance, and heart disorders. Insomnia, the most prevalent sleep disorder, requires more effective diagnosis and screening for proper treatment. Actigraphic data and its combination with physiological sensors like electroencephalogram (EEG), electrocardiogram (ECG), and body temperature have proven significant in predicting insomnia using machine learning methods. Studies focusing solely on actigraphic data achieved an accuracy of 84%, combining it with other wearable devices increased accuracy to 88%, and 2-channel EEG alone yielded an accuracy of 92%, but limits scalability and practicality in real-world settings. Here we show that using the hybrid approach of incorporating both recursive feature elimination (RFE) and principal component analysis (PCA) on sleep and heart data features yields outstanding results, with the multi-layer perception (MLP) achieving an accuracy of 95.83% and an F1 score of 0.93. The top-ranked features are predominantly sleep-related and time-domain RR interval. The dependent variables in our study have been extracted from the self-report Pittsburgh Sleep Quality Index questionnaire responses. Our findings emphasize the importance of tailoring feature sets and employing appropriate reduction techniques for optimal predictive modeling in sleep-related studies. Our results demonstrate that the ensemble classifiers generalize well on the dataset regardless of the feature count, while other algorithms are hindered by the curse of dimensionality.

Wastewater must be treated before discharge into the recipient to such an extent that it meets standards and regulations on wastewater quality, so as not to damage the environment. Depending on the quality of the influent, different technological procedures are applied, which are more or less energy intensive. Also, with the tightening of the conditions related to the quality of the effluent, the application of more energy-intensive purification technologies occurs, and thus the energy consumption at the plants increases. Wastewater treatment plants are among the biggest consumers of energy. In this paper, electric energy consumption at wastewater treatment plants was analyzed depending on different indicators of specific energy consumption, the applied technological process, and the level of purification.

Introduction: 2,4-Diaminobutyric acid (2,4-DABA) is an excitatory amino acid with neurotoxic, hepatotoxic, and potentially carcinogenic effects, found in aquatic ecosystems with a tendency to accumulate in plants and animals. Due to its potential impact on development, its embryotoxicity is being studied. Zebrafish (Danio rerio), with high homology to the human genome, serve as an excellent model for studying development and developmental abnormalities. Objective: To investigate the effect of 2,4-DABA on proteins crucial for zebrafish eye development using molecular docking. Methods: Proteome screening was conducted using the FINDSITEcomb software, selecting 1119 proteins based on homology, tissue specificity, and expression timing. Six proteins that met the criteria were analyzed using molecular docking in the AutoDock Vina program. Results: The interaction of the fzd8a protein with 2,4-DABA showed the lowest Gibbs free energy value of -4.6 kCal/mol, while the interaction with the pbx4 protein had the highest value of -3.4 kCal/mol. A similarity was observed in the amino acid sequence of proteins that bind to 2,4-DABA, particularly in those containing an -SH group. Conclusion: 2,4-DABA may affect eye structure development in zebrafish by interacting with proteins throughout the entire development period. The results of in silico analyses provide a basis for further in vivo research, which should be conducted on live embryos to confirm these findings.

Introduction/Aim: Procaine is a local anesthetic from the amino ester group that blocks voltage-gated sodium channels. Since it can cross the placenta in its ionized form, its potential impact on embryonic development is of concern. Zebrafish, which have a significant degree of homology with the human genome, allow for the study of cardiovascular system development with reliable extrapolation to humans. Investigation of the effects of procaine on key proteins involved in zebrafish heart development using molecular docking. Methods: First, a screening of the interaction between procaine and the entire human proteome was performed using FINDSITEcomb software. Based on significant interactions with a high degree of analysis precision, 113 proteins were selected. Using the ZFIN database, the degree of homology between the selected human proteins and zebrafish proteins, tissue specificity, and expression timing were determined. Nine proteins met all the criteria: kcnh6a, kcnh7, kcnh5a, kcnh2a, psen2, rbfa, and zfpl1, and were further investigated through molecular docking in the AutoDock Vina program. Results: Most of the proteins were highly expressed during the blastula stage. Docking results showed that the scn1laa protein and procaine had the lowest Gibbs free energy value (-6 kCal/mol), while the zfp11 protein had the highest value (-4.4 kCal/mol). Procaine binding to the tested proteins revealed similar amino acid sequences within the same protein family. Conclusion: Procaine interacts with proteins involved in zebrafish heart development under in silico conditions. Further analyses on live embryos are needed to complement these findings.

Myocardial infarction (MI) is a serious cardiovascular disease and the primary cause of mortality, with a complex etiopathology. Identifying the genetic basis of myocardial infarction (MI) is essential for developing personalized medical treatments. This study examined the possible association between polymorphisms in the methylenetetrahydrofolate reductase (MTHFR) gene and MI. In the study, 120 patients with MI and 120 age-and-sex-matched controls were genotyped for C677T and A1298C MTHFR polymorphisms by the allele-specific or amplification refractory mutation systempolymerase chain reaction (ARMS-PCR). In the case of the C677T polymorphism, the T/T and C/T genotypes were associated with a significantly increased risk of MI under the dominant genetic model (odds ratio (OR)=2.060; P=0.006). Although there was no significant association between the A1298C variant and MI, this polymorphism was linked to a higher level of creatinine in MI patients (P<0.002). A similar association was observed for the C677T polymorphism (P=0.003). An A-T haplotype represented an increased risk for MI (OR=1.630; P=0.014), whereas the A-C haplotype had a protective role (R=0.517; P=0.002). These findings indicate that C677T MTHFR polymorphism is strongly associated with and increased risk of MI, making it a potential genetic risk factor and a possible predictor of MI.

In medieval Bosnia, the existence of a unified legal code that defined the legal principles, duties, and obligations of the parties involved in a particular dispute was not recorded. One of the most significant relationships in the history of medieval Bosnia was the relationship between the ruler and the nobility. The rights and obligations of the nobles in relation to the ruler in the medieval Bosnian state were defined through the institution of "faithful service" on one side and "noble faith" on the other. Simply put, as long as the nobles faithfully served the ruler, they were secure and their possessions were protected. This article focuses on instances of treason against the ruler and the judicial body responsible for investigating charges and delivering judgments according to established legal customs. The nobles mentioned in the Bosnian charters played a role in defining the relationship between the ruler and the respective noble, as did members of the hierarchy of the Bosnian Church. The Bosnian Church was a religious institution formed in the territory of Bosnia after the displacement of the Catholic diocese in the mid-13th century and was first mentioned in the 1320s. It was considered a heretical movement by the Roman Church, and its followers were excommunicated. It was accepted by the Bosnian ruling dynasty of Kotromanić and by the majority of noble families. From the perspective of neighboring states, the hierarchy of this church was considered to be the authority characterized by exceptional moral values. The aim of this article is to provide the research results regarding the role of the head (djed) of the Bosnian Church in determining the culpability of Bosnian nobles during the 14th and 15th centuries.

This study aims to assess and monitor the health of an urban protected area by analyzing the levels of persistent organic pollutants (POPs) and mercury (Hg) in soil and sediments. Based on the results, the detected concentrations of organochlorine pesticides (OCPs), polychlorinated biphenyls (PCBs), polycyclic aromatic hydrocarbons (PAHs), and Hg are above the threshold maximum values for soils, and the prescribed target values for sediments. In the investigated protected area PCBs pose a very high ecological risk. The presence of 16 priority PAHs in analyzed soils and sediments poses a moderate to high cancer risk and Hg poses a considerable health risk to children. The research suggests that preserving urban protected areas is crucial for environmental and urban sustainability. In urban environments these areas should be evaluated in terms of their environmental, eco-geochemical, economic, and socio-cultural dimensions. The value of the existence of this natural oasis lies in its aesthetic and psycho-hydrological impact, local climate regulation, residential isolation, and significant art-architectural and horticultural shaping. The connection between eco-geochemical and management practices, planning, and urban green spaces policy should become an adopted innovation in the cities in the future.