Lungs are among the most common sites for development of both primary and metastatic carcinomas. Tumor cells expression (TC) of PD-L1 is an important predictor of the of response to immune check-point inhibition in NSCLC, while the composition of the immune cells (IC) in the tumor microenvironment including PD-L1+ cells is believed to predict responses in tumors of some other primary sites. Total mutational load (TML) and microsatellite instability (MSI) also play a role in response to the immune checkpoint blockade. We investigated immune microenvironment characteristics (PD-1, PD-L1, CD8) of 257 lung biopsies including 81 primary (NSCLC) and 176 metastatic tumors to the lungs. TML and MSI were calculated from massively parallel sequencing (592-gene panel). TC expression of PD-L1 was more common in NSCLC than in metastatic carcinomas (28% vs. 10%, p=0.009), while PD-L1-positive IC were present at relevant percentages (1-5%) exclusively in metastatic carcinomas (31% IC positive vs. 0%, p<0.001). Metastatic carcinomas carried significantly lower TML in comparison with the NSCLCs (6.6 mutations on average vs. 10, p=0.01). All primary NSCLC were microsatellite stable, and only 2 metastatic carcinomas exhibited MSI-H status. The number of PD-1+ and CD8+ tumor infiltrating lymphocytes did not differ significantly between the primary and metastatic carcinomas. Our study revealed significant differences in tumor immune microenvironment (PD-L1 in IC and TC), and its relationship to TML between NSCLC and metastatic cancers. These differences could determine the choice of a predictive biomarker test and subsequently effect(s) of the immune therapy treatments in various advanced cancers.

Abstract Knautia sarajevensis is an endemic plant of the Dinaric Alps and is mainly distributed on Bosnian Mountains. Due to the quite large flower heads and easy maintenance, this plant has a potential use as a substitute ornamental plant for K. arvensis in perennial beds. The current study evaluated the germination process in different treatments in an attempt to suppress dormancy and increase germination rate, and to develop a successful protocol for micropropagation. An over 60% germination rate was achieved through cultivation of seeds on MS basal medium with reduced mineral nutrient composition and the absence of sucrose. On the other hand, a below 10% germination rate was achieved with untreated seeds. Suppression of apical dominance was achieved through application of high concentrations of kinetin, apical shoot decapitation or cultivation of shoots in liquid media. Overall, liquid cultures were more successful as a micropropagation system for this plant. Shoots spontaneously developed roots on multiplication treatments and were successfully acclimatized. Moreover, phenolic compound profile was analysed in the light of the possible medicinal potential of this plant. Variable amounts of total phenolic compounds as well as individual phenolics were recorded, according to treatment and solidification of media. An increase in rosmarinic acid content was reported for kinetin treatments and acclimatized plants comparing to mother plants in natural habitat. The present study shows that choice of cytokinin concentration, explant type as well as culture type influences not only shoot proliferation and apical dominance suppression but also in vitro production of phenolics.

The aim of this paper was to identify differences in the perception of social support for young people with and without disabilities in the age of 18-35 in the Tuzla Canton. The sample of examinees consists of two sub-classes. The first subsample is made of young people with disabilities, chronological age 18-35, both sexes (N = 50). The second subsample consists of young people with no disabilities, chronological age 18-35, both sexes (N = 50). The data obtained by the research were processed by the method of parametric and nonparametric statistics. The frequencies, percentages and measures of central tendency (arithmetic mean, standard deviation) are calculated. P-values were used to investigate the differences between the examined variables, and the variance analysis was used to investigate the significance of differences. The results of research on differences in the perception of social support among young people with disabilities and young people without disabilities show that there is a statistically significant difference in the most applied variables. Based on the results obtained in the case of youth with disabilities, it would be advisable to start with integration into social life at all levels.

<p style="text-align: justify;">This paper deals with the resistance of the shed of a steel hall in Drvar according to technical regulations for snow from 1961 and EC1 regulations. The paper aims to check the reliability of the shed with probabilistic approach. That also implies calibration of the steel hall construction analyzed with classic deterministic method. Statical system of the shed of a steel hall is a beam with a given span, Figure 1. Steel sections are hot rolled INP sections with specified statistic parameters. The length of the beam span is &lambda; = 2,4 m. The reliability indexes have to be analyzed in point 1(relevant statical forces and displacements for ULS and SLS) and according to equations of limit states.</p>

<p style="text-align: justify;">The aim of this paper was to investigate the connection between student motivation and Content and Integrated Language Learning (CLIL) approach at the technically-oriented faculties of the University of Zenica. The past few decades have been marked with ongoing debates on what teaching approaches are most motivating for foreign language learners. It has been agreed upon that student-centered approaches are the most efficient ones. CLIL is one of them. Inherently, CLIL uses foreign language for teaching a particular content, in our case &ndash; the engineering one. For the purpose of the research, a questionnaire was conducted among the CLIL students, subsequent to their conference - CLIL 2017, which is the last stage of the CLIL approach to English language teaching at the aforementioned faculties. We hypothesized that the CLIL approach bolsters student<br />motivation for English language learning more than the ex-cathedra approach. The questionnaire proved the hypothesis true as the results indicated high level of motivation in students. In addition, students expressed great satisfaction with the CLIL in terms of its effect on their foreign language proficiency as well as the knowledge in the content matter<br />related to engineering. Also, students seem to be well aware of the fact that good knowledge of English language and good knowledge of engineering content create a solid basis for employment.</p>

<p style="text-align: justify;">The paper analyzes the behavior of the plastic container during the buckling, ie during the load effect on the containers. The analysis was experimentally performed on several different types of containers. The container material is polypropylene. Experimental determination of pressure force and corresponding deformation was performed in the<br />laboratory at the Faculty of Technical Engineering Bihać. The analysis includes experimental testing on assembled containers and on container side. Places where deformation occurs on the container sides are shown.</p>

In order to assess the genetic purity of common buckwheat variety ‘Darja’ which is the most commonly produced variety of this crop in Bosnia and Herzegovina, 10 SSR markers have been used. Five samples have been collected from different production regions in B&H (Breza, Nisici Plateau, Ustikolina, Bihac and Bosanska Krupa) and compared to the reference ‘Darja’ sample obtained from an ex situ seed collection from Slovenia. Seven out of ten primer pairs used managed to amplify SSR alleles. Analyses of molecular variance (AMOVA) showed a significant differentiation between the reference and all analyzed ‘Darja’ samples. Furthermore, the factorial correspondence analysis revealed a clear differentiation between the reference and ‘Darja’ samples from the most known production regions of common buckwheat in B&H clustering four out of five analyzed samples very close together. The most divergent one among the analyzed samples was the one from Ustikolina. Genetic purity of varieties of all of cross pollinated species produced in Bosnia and Herzegovina is questionable due to the general use of farm-saved seeds.

The expert reports state that Bosnia and Herzegovina, despite the presence of diverse and valuable natural resources, lacks systematic, coordinated and harmonized pipeline for biomonitoring. Successful solutions to serious problems regarding environmental protection, management and research rely on the efficient use of exhaustive and unfailing information on the nature around us. However, more often than not, transitional and developing countries lack any centralized, nationally funded databases that could be used as dependable source of information in decision making process. University of Sarajevo-Institute for Genetic Engineering and Biotechnology (INGEB) developed the Regional Biodiversity Database – REBIDA with the aim to collate all known biological data on wild and domesticated natural resources of Bosnia and Herzegovina. This internet-based database represents a comprehensive, searchable and open access platform for science community, academia, governmental and non-governmental stakeholders and general public. Besides its scientific value, REBIDA will serve as an educational tool for discovering the diversity and importance of natural resources, with special emphasis on indigenous and endemic flora, fungia and fauna from the Balkans. It is the only such database in the country, consisting of three functionally connected segments: tissue database, DNA database and digital genetic database on plant, animal and human samples. To complement REBIDA, a mobile application called REBIDA SCANNER was also developed. It will be free to download for IOS and Android platforms and will enable professionals, nature enthusiasts and any other interested parties to contribute to REBIDA through data collection, field sampling and documentation of B&H wild life.

The region of Western Balkans has been inhabited since the Paleolithic era and was the route of the spread of farming from the Middle East to Europe during the Neolithic era. In the present study, Y-STR data from European populations have been used to construct median-joining networks. The study was performed using Whit Athey’s Haplogroup Predictor, Y Utility and Network 4 software packages to predict Y haplogroups, construct networks, perform clustering of closely related Y chromosomes and calculate time estimates between individual nodes. The results of the study imply that geographically close populations cluster together at both Balkan and European levels. It was observed that an elevated number of study populations and individual haplogroups increases the possibility that individuals of different ethnic background cluster within the same or neighboring clades of network. Subsequent time estimates, performed based on the mutation frequency between the ancestral node and its descendant nodes, revealed that I2a haplogroup within the Western Balkan region has the most compact clustering (age, estimated at 3109 years), followed by Hg E1b1b which has the second most compact clustering (4896 years). The obtained results are nonetheless in accordance with previously published research investigating the frequency of Y haplogroups based on Y-SNP variant frequencies, indicating that Western Balkan countries are mainly represented by I2a subclade (average for six countries 32.3%), followed by E1b1b and R1a (average for six countries of 21.5% and 17%, respectively).