UDK 581.19:547.56]:582.736.3 The aim of this study was to determine the total concentrations of some phenolic compounds and antioxidant and antimicrobial activity of methanol extracts of different parts of Illyrian endemic Petteria ramentacea. Concentrations of phenolic compounds were determined with UV/VIS spectrophotometry. The antioxidant activity of plant extracts was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. Antimicrobial activity of extracts was evaluated by measuring the inhibition's zone against six selected test bacteria and two fungi. The highest average total phenols concentrations were in seeds (10.78 mg GAE g-1 DW), root (10.51 mg GAE g-1 DW) and bark (10.40 mg GAE g-1 DW), and the lowest in inflorescences (2.99 mg GAE g-1 DW) and leaves (3.12 mg GAE g g-1 DW). The total flavonoids concentrations were determined only in leaves (8.25 mg CE g-1 DW) and in stem (5.66 mg CE g-1 DW). Both flavanols and proanthocyanidins (0.75 mg CE g-1 DW and 3.49 mg CE g-1 DW, respectively) were found only in leaves. Analysis of variance indicated presence of significant differences between total phenols and flavanols concentrations (p<0.05), and Duncan's test confirmed the presence of intraspecies variability according to their concentrations. The highest antioxidant activity (IC50) was observed for the seed's extract (6.86%) and the lowest for the bark’s (51.31%). All methanol extracts showed the most pronounced antibacterial activity against Staphylococcus epidermididis, and the lowest against S. aureus subsp. aureus. Antifungal activity against Candida albicans was moderate. Since Peteria ramentacea methanol extracts are potential natural antioxidant and antimicrobial preparations against selected microorganisms, it is necessary to continue with more detailed analysis.

Abstract The main objective of endodontic treatment is to remove vital and necrotic remnants of pulp tissue and microorganisms and their toxic products from the root canal. During chemo-mechanical endodontic preparation, a smear layer is formed on the wall of the canals. Due to an inability to remove all tissue remnants and the smear layer from the root canal by mechanical instrumentation, it is necessary to use irrigation to ensure sufficient cleaning and disinfection of the largest part of the root canalicular system. The most commonly used irrigants are sodium hypochlorite (Na-OCl), ethylenediaminetetraacetic acid (EDTA), citric acid and chlorhexidine (CHX). Recently, the irrigants QMix and MTAD have been introduced to the market. They are a mixture of different components having antimicrobial, organolytic and mineralytic effects on canal detritus and the smear layer. This review article investigates irrigants in terms of the nature of their effect, their efficiency, optimal concentration, and method of use, and the interactions between the irrigants most commonly used in endodontic therapy are discussed, with special emphasis on QMix and MTAD.

Abstract The paper provides an overview of the trend in the concentration of the total assets of banks in Croatia for the period from 2007 to 2016 with the aim of analysing and presenting the changes that occurred in the system. Also, the paper shows the theoretical framework of the indicators used in the research as well as the comparison of their obtained values. The data used to calculate the total assets concentration are taken from the Croatian National Bank. The concentration indices used in the study include the entropy measure, the Theil entropy, the Gini coefficient, the Pietra index, the Atkinson index and the coefficient of variation. The results indicate a very slight decrease in concentration over the past several years, while the coefficient of variation points to the heterogeneity of the system, as well as to inequalities among the banks, which are most evident in the size of banks assets.

Even though coordinate measuring machines (CMM) still achieve the most accurate measurement results, non-contact (optical) measuring systems are applied more and more in industry. The reasons of using optical scanners are in the higher speed of acquisition, higher density of data-points and better surface description, the ability to scan complex and non-rigid surfaces etc in comparison to contcact devices. This paper gives a review of state-of-the-art measuring contact and non-contact inspection technologies in industrial applications. Listed are the devices, principles and systems that are used at the data-acquisition (triangulation, time-of-flight and interferometry). The description of contact measuring machines (portable CMM and stationary CMM) and devices for non-contact scanning (laser scanner, structured light scanner and CT scanner) is given, and their advatages and disadvantages are mentioned with corresponding literature review.

Abstract Websites are one of the most widely distributed information resources. Educational institutions use this resource to ensure that the best quality of information transmission is achieved. As such, academic sites have become a very important aspect of academic institutions, one that affects their overall quality. Bearing in mind the importance of university websites’ quality, the authors of this paper presented a multi-criteria model for evaluating the quality of university websites. This paper presents the hybrid IR-AHP-MABAC (Interval Rough Analytic Hierarchy Process - MultiAttributive Border Approximation Area Comparison) model. The model is adapted to group decision making as based on the application of a new approach to treating uncertainties through the use of interval rough numbers (IRN). The modified IR-AHP method was used to determine the weight coefficients of the criteria in the group decision-making process. The results of the IR-AHP model are compared with results provided by the traditional AHP method and the fuzzy AHP approach. The IR-MABAC model was used for the evaluation of university websites. In order to verify the results of the IRN based approach, the IR-MABAC model was compared to the F-TOPSIS (Fuzzy Technique for Order of Preference by Similarity to Ideal Solution), F-VIKOR (Fuzzy MultiCriterion Optimization and Compromise Solution), F-COPRAS (Fuzzy COmpressed PRoportional ASsessment), F-MAIRCA (Fuzzy MultiAtributive Ideal-Real Comparative Analysis), and F-TODIM (an acronym of Interactive and Multi Criteria Decision Making in Portuguese) models. The credibility of the IR-AHP-MABAC model was demonstrated by comparing the results of different multi-criteria techniques and analyzing viability. The results of the IRN approach and fuzzy comparison have shown that the new approach to dealing with imprecision yields credible, reputable ranks.

Summary Introduction Caries and orthodontic anomalies in school-age children lead to disturbed aesthetics, oral functions (chewing, swallowing, and speech), predisposition to trauma and the onset of periodontal diseases. The aim was to assess dental health and frequency of orthodontic anomalies in children aged 8-9 years in the municipality of Foča. Methods The research was conducted in the primary school Sveti Sava in Foča, where the total of 112 children age 8-9 years were examined. An informed consent was obtained from parents and school director for each student. Dental examination was performed using standard dental method, a mirror and a probe under artificial lighting. Children received instructions on proper nutrition, oral hygiene, tooth protection and elimination of bad habits. Results Among 112 examined boys and girls of selected ages, very high person caries index (PCI) was found (78.57%). A total of 548 caries affected teeth were found (boys 331 (24.62%), girls 217 (16.14%) (p <0.05)). Fifty-nine children had caries lesions on permanent teeth (boys 39 (2.9%), girls 20 (1.5%) (p <0.05)). Sagital abnormalities of the bite were present in 39 (34.82%) children, while 38 (33.92%) anomalies were related to vertical bite irregularities, 10 (8.92%) of them had open bite and 28 subjects (25%) had deep bite (p> 0.05). Conclusion Large number of teeth was affected with caries lesions (548) in eighth-year-olds, while orthodontic anomalies, mostly sagital abnormalities of bite were found in 39 subjects.

Aerogels are the least dense and most porous materials known to man, with potential applications from lightweight superinsulators to smart energy materials. To date their use has been seriously hampered by their synthesis methods, which are laborious and expensive. Taking inspiration from the life cycle of the damselfly, a novel ambient pressure‐drying approach is demonstrated in which instead of employing low‐surface‐tension organic solvents to prevent pore collapse during drying, sodium bicarbonate solution is used to generate pore‐supporting carbon dioxide in situ, significantly reducing energy, time, and cost in aerogel production. The generic applicability of this readily scalable new approach is demonstrated through the production of granules, monoliths, and layered solids with a number of precursor materials.

BACKGROUND Although agility is an important quality in basketball, factors associated with basketball specific pre-planned-agility (change-of-direction-speed, CODS) and non-planned-agility (reactive agility, RA) are rarely investigated. The aim of this study was to evaluate relationship between anthropometric and motor indices with basketball-specific CODS and RA in male basketball players of high performance level. METHODS We tested 88 high-level male basketball players (height: 194.62±8.09 cm; body mass: 89.13±10.81 kg; age: 21.12±3.47 years). The sample was randomly divided into validation (N.=44) and cross-validation (N.=44) subsamples. The study variables included: broad-jump, countermovement-jump, reactive-strength-index, visual-reaction-time, body height, body mass, and body fat percentage (predictors); as well as basketball-specific CODS and RA (criteria). Univariate associations were assessed by Pearson's correlation coefficients. Multivariate relationships between the predictors and the criteria were assessed with multiple regression analysis for the validation subsample, which was then cross-validated. RESULTS The established multiple regression models were successfully cross-validated for CODS (R2=0.40 and 0.36; P=0.01) and RA (R2=0.38 and 0.41; P=0.01, for validation and cross-validation subsample, respectively). The broad-jump (i.e., horizontal displacement) is important predictor of CODS (Beta=-0.41; P=0.01); anthropometrics and body build are specifically associated with RA (Beta=0.51, -0.61 and 0.41 for body height, body mass and body fat percentage, respectively; all P<0.05), while reactive-strength-index is directly related both to CODS (Beta=-0.41, P=0.02), and RA (Beta=-0.40, P=0.03). CONCLUSIONS While basketball players are differentially oriented toward specific game duties, specific capacities should be developed in order to meet specific sport requirements.

Introduction Since the discovery and clinical success of the platinum(II) anticancer drug, cisplatin, researchers are putting much effort to develop more efficient metal-based therapeutic compounds, with fewer side-effects and greater cytoselectivity. Ruthenium complexes arose as promising anticancer agents, due to the success of some ruthenium drug candidates in clinical trials. Here we report comparison of in vitro cytotoxic activity and mechanisms of action of cisplatin and four newly synthesised ruthenium(III) complexes with bidentate anionic schiff base derived from 5-methylsalicilaldehyde and methylamine: (complexes 1– 4). Material and methods Cytotoxicity was tested on four human cancer cell lines (K562, A549, EA.hy926, MDA-MB231) and one human non-tumour cell line (MRC-5), by MTT assay. Being the most cytotoxic of all four tested complexes, complex 1 (C1) (Na[RuLCl2], L=N-propyl-5-chlorosalicideniminato) is selected for further analyses of molecular mechanisms underlying its activity toward MDA-MB231 cells. Results and discussions The average IC50 values were in the low micromolar range 2–23 µM, depending on cell line. Investigated complexes displayed an apparent cytoselective profile, as they reduced the viability of tested tumour cell lines more efficiently than of the non-tumour MRC-5 cells. Cisplatin resistant MDA-MB231 cells showed to be ten times more sensitive to C1 (IC50=2 µM) than to cisplatin. 24 hour treatment of MDA-MB231 cells with IC50 values of C1 and cisplatin induced minor cell cycle alterations, while 48 hour treatment induced substantial accumulation of cells in Sub-G1 region, up to 22.4% (C1) and 86.4% (cisplatin), versus control 4.8%. Acridine orange/ethidium bromide dual staining confirmed the Annexin V-FITC/PI assay results of notable reduction in cell number after the treatment with C1 and cisplatin. While cisplatin-treated cells prominently die of necrosis, C1-treated cells after 24 hour treatment show apoptotic morphology, but after prolonged treatment, necrosis becomes predominant. Decrease in the intracellular levels of reactive oxygen species was comparable in the cisplatin-treated and C1-treated cells, with cisplatin displaying more conspicuous effects at higher dose. C1 entered the cells more efficiently compared to cisplatin. Intracellular C1 concentration after 4 hour treatment exceeded that of cisplatin by 7.8 times approximately. Conclusion Present study pointed out interesting activity of this type of ruthenium(III) complex and need for further biological studies and its chemical structure optimisation.

Background Chagas disease (CD) is a major public health concern in Latin America and a potentially serious emerging threat in non-endemic countries. Although the association between CD and cardiac abnormalities is widely reported, study design diversity, sample size and quality challenge the information, calling for its update and synthesis, which would be very useful and relevant for physicians in non-endemic countries where health care implications of CD are real and neglected. We performed to systematically review and meta-analyze population-based studies that compared prevalence of overall and specific ECG abnormalities between CD and non-CD participants in the general population. Methods Six databases (EMBASE, Ovid Medline, Web of Science, Cochrane Central, Google Scholar and Lilacs) were searched systematically. Observational studies were included. Odds ratios (OR) were computed using random-effects model. Results Forty-nine studies were selected, including 34,023(12,276 CD and 21,747 non-CD). Prevalence of overall ECG abnormalities was higher in participants with CD (40.1%; 95%CIs=39.2-41.0) compared to non-CD (24.1%; 95%CIs=23.5-24.7) (OR=2.78; 95%CIs=2.37-3.26). Among specific ECG abnormalities, prevalence of complete right bundle branch block (RBBB) (OR=4.60; 95%CIs=2.97-7.11), left anterior fascicular block (LAFB) (OR=1.60; 95%CIs=1.21-2.13), combination of complete RBBB/LAFB (OR=3.34; 95%CIs=1.76-6.35), first-degree atrioventricular block (A-V B) (OR=1.71; 95%CIs=1.25-2.33), atrial fibrillation (AF) or flutter (OR=2.11; 95%CIs=1.40-3.19) and ventricular extrasystoles (VE) (OR=1.62; 95%CIs=1.14-2.30) was higher in CD compared to non-CD participants. Conclusions This systematic review and meta-analysis provides an update and synthesis in this field. This research of observational studies indicates a significant excess in prevalence of ECG abnormalities (40.1%) related to T. cruzi infection in the general population from Chagas endemic regions, being the most common ventricular (RBBB and LAFB), and A-V B (first-degree) node conduction abnormalities as well as arrhythmias (AF or flutter and VE). Also, prevalence of ECG alterations in children was similar to that in adults and suggests earlier onset of cardiac disease.

BackgroundHBsAg immune-escape mutations can favor HBV-transmission also in vaccinated individuals, promote immunosuppression-driven HBV-reactivation, and increase fitness of drug-resistant strains. Stop-codons can enhance HBV oncogenic-properties. Furthermore, as a consequence of the overlapping structure of HBV genome, some immune-escape mutations or stop-codons in HBsAg can derive from drug-resistance mutations in RT. This study is aimed at gaining insight in prevalence and characteristics of immune-associated escape mutations, and stop-codons in HBsAg in chronically HBV-infected patients experiencing nucleos(t)ide analogues (NA) in Europe.MethodsThis study analyzed 828 chronically HBV-infected European patients exposed to ≥ 1 NA, with detectable HBV-DNA and with an available HBsAg-sequence.The immune-associated escape mutations and the NA-induced immune-escape mutations sI195M, sI196S, and sE164D (resulting from drug-resistance mutation rtM204 V, rtM204I, and rtV173L) were retrieved from literature and examined. Mutations were defined as an aminoacid substitution with respect to a genotype A or D reference sequence.ResultsAt least one immune-associated escape mutation was detected in 22.1% of patients with rising temporal-trend. By multivariable-analysis, genotype-D correlated with higher selection of ≥ 1 immune-associated escape mutation (OR[95%CI]:2.20[1.32–3.67], P = 0.002). In genotype-D, the presence of ≥ 1 immune-associated escape mutations was significantly higher in drug-exposed patients with drug-resistant strains than with wild-type virus (29.5% vs 20.3% P = 0.012). Result confirmed by analysing drug-naïve patients (29.5% vs 21.2%, P = 0.032). Strong correlation was observed between sP120T and rtM204I/V (P < 0.001), and their co-presence determined an increased HBV-DNA.At least one NA-induced immune-escape mutation occurred in 28.6% of patients, and their selection correlated with genotype-A (OR[95%CI]:2.03[1.32–3.10],P = 0.001).Finally, stop-codons are present in 8.4% of patients also at HBsAg-positions 172 and 182, described to enhance viral oncogenic-properties.ConclusionsImmune-escape mutations and stop-codons develop in a large fraction of NA-exposed patients from Europe. This may represent a potential threat for horizontal and vertical HBV transmission also to vaccinated persons, and fuel drug-resistance emergence.

Background Juvenile systemic scleroderma (jSSc) is an orphan disease, with an estimated prevalence of 3 per 1000 000 children. Most jSSc patients primarily present with Raynaud phenomenon (RP). We investigated in our patient of the juvenile scleroderma inception cohort, how fare patients with (RP+) and without (RP-) RP differed in their clinical presentation at enrolment. Methods The jSSc is a prospective cohort of jSSc patients. Patients were enrolled who were diagnosed with jSSc, had a jSSc onset age under 16 years and were younger as age of 18 years at the time of inclusion. The patients are prospectively assessed every 6 months according to a standardised protocol. We reviewed the organ involvement pattern of our patients currently followed in the cohort. Results 100 patients are currently followed in the cohort and 89 (89%) of them had RP. The female/male ratio was lower in the RP +group, 3.7:1 compared to 4.5:1(p=0.808). Diffuse subtype was more common in the RP +group, 72% compared to 63%. Mean age of onset of first non- Raynaud symptomatic was 10.4 years in both groups. Mean disease duration was slightly higher in the RP +group, 3.4 compared to 2.2 years. ANA positivity was higher in the RP +group, 88% compared to 70% (p=0.48). Anti-Scl70 was 34% in the RP +and 20% in the RP-group (p=0.34). Interestingly 7% of RP +but none of the RP +were anti-centromere positive. The mean modified skin score was lower in RP +group (mean of 14.8 compared to 17.0). There were significantly more nailfold capillary changes (70% compared to 18%, p=0.001) and a higher rate of history of ulceration in the RP +group (49% compared to 20%, p=0.083). Decreased DLCO and FVC <80% was higher in the RP-negative group with 45%/50% compared to 37.5%/31% respectively. Pulmonary hypertension occurred in 7% in the RP +group and there was no case in the RP- group (p=0.335). RP- group had a higher rate of urinary sediment changes 18% compared to 4.5% in the RP +group (p=0.07). No renal crisis or hypertension was reported in neither groups. Gastrointestinal involvement was similar between the two groups with around 35%. Occurrence of swollen joints was similar in both groups as the frequency of muscle weakness with around 20%. The tendon friction rub occurred around 10% in both groups. In the patient related outcomes, there was only a difference in rating of Raynauds activity. Conclusions The RP– group differed from RP +group in the clinical presentation at enrolment. The absence of Raynaud phenomenon was associated with a decreased rate of history of ulceration, no occurrence of pulmonary hypertension. Interestingly higher rate of urinary sedimentary changes and no anticentromere positivity was observed in RP- patients. Disclosure of Interest None declared

The pathogenesis, clinical course, and response to treatment in systemic juvenile idiopathic arthritis (SJIA) differ from other types of juvenile idiopathic arthritis and are similar to other interleukin-1 (IL-1)-mediated diseases. The main cytokine involved in the pathogenesis of SJIA is IL-1β, which can be neutralized by targeted anti-IL-1 therapy. In SJIA, no antibodies have been found and there is growing evidence that it is mainly an autoinflammatory and not an autoimmune disease. Before the era of biologic therapy, treatment of SJIA was primarily based on long-term treatment with high doses of glucocorticosteroids (GCS). The side effects of GCS could have a significant impact on the outcome of the disease and could cause long-term damage. Treatment with anti-IL-1 agents early in the disease course has revolutionized the management principles of SJIA. However, not all SJIA patients respond equally well to anti-IL-1 therapy, and it has been shown that age at the onset of disease, duration of the disease, number of affected joints, neutrophil count, and ferritin level can predict the response to anti-IL-1 therapy. In particular, an elevated ferritin level should prompt testing for macrophage activation syndrome (MAS), the most severe complication of SJIA. Anti-IL-1 therapy has been shown to be effective also in patients with MAS. Although anti-IL-1 agents are currently not recommended as first-line treatment, there is growing evidence that anti-IL-1 agents introduced at the beginning of SJIA could enable lower doses and a shorter duration of GCS therapy, change the long-term disease outcome, and even influence molecular disease patterns. There are currently three anti-IL-1 agents available: anakinra, canakinumab, and rilonacept. In this review, we present the current knowledge on the pathogenesis of SJIA, the rational for anti-IL-1 treatment, and future perspectives on the treatment of SJIA.