Case summary A 2-year-old domestic longhair crossbred female cat was referred for a second opinion on a non-healing surgical wound after left eye enucleation. In addition to the left orbital lesion, ulcerative granular masses protruding from the left nostril and on the base of the left ear were noted. A diagnosis of cryptococcosis was established using histopathological examination and a latex cryptococcal antigen agglutination test. The cat was successfully treated with itraconazole. Relevance and novel information Cryptococcosis, commonly reported in Australia, western Canada and the western USA, is rarely reported in companion animals in Europe. This marks the first report of cryptococcosis in cats in Bosnia and Herzegovina, emphasising the need to raise awareness within the veterinary community, both local and regional, about this disease.

An atrioventricular valve insufficiency is a common form of cardiac disorder in mammals, whereas it has rarely been reported in reptiles. A two-year-old male bearded dragon (Pogona vitticeps) was presented with acute onset of lethargy, inappetence and general weakness, increased respiratory effort, dark skin color, and arched back. A gastrointestinal foreign body was suspected, but radiography showed coelomic fluid accumulation. Ultrasound heart examination revealed turbulent blood flow at the level of the atrioventricular valves. Electrocardiography by modified Einthoven lead system (lead configuration 1) as described for Squamata, did not show significant changes. A tentative diagnosis of congestive heart failure as a result of valvulopathy was made. Treatment consisted of parenteral furosemide and fluid administration and assisted feeding with appropriate food intended for adult bearded dragons. The patient was weekly evaluated and successfully recovered over the four months. Echocardiography and electrocardiography were repeated 12 months later. The prescribed treatment was successful with complete resolution of clinical signs, and long-term surveillance of the bearded dragon (Pogona vitticeps).

In previous years interest has grown in investigating the attitudes and capabilities of veterinarians regarding the recognition, quantification and treatment of animal pain throughout different parts of the world and encompassing various species. This is the first report exploring the attitudes and self-rated abilities of veterinarians in Bosnia and Herzegovina (B&H) concerning recognition and quantification of pain in domestic animals. A study questionnaire was made available to 535 general practice veterinarians throughout B&H and 73 (14%) responded in full. The questionnaire contained polar, multiple choice, ordinal and interval scale questions and consisted of sections asking about demographic data, attitudes to pain recognition and quantification, use and availability of analgesics, estimates of pain intensity during specific surgical procedures, and the perceived need for pain assessment and continuing education programmes for analgesia. Half of the respondents considered the recognition and quantification of pain to be difficult while 89% did not make use of pain assessment scales. Of the respondents, (33/73; 45%) felt a certain level of pain to be advantageous since it reduces the activity of the healing animal, whereas 52% (38/73) did not agreed with this concept. Cost was a consideration when deciding whether or not to use analgesics for 58% (42/73) of the respondents with the most commonly used types being NSAIDs (72/73;99%) and opioids (60/73; 82%). Practitioners in B&H displayed awareness of the importance of pain assessment and management however a significant proportion were unaware of pain scales and relied upon physiological indicators of pain.

Little is known about vaginal and uterine bacteria in clinically healthy cats and their correlation with different stages of the oestrus cycle. The differences in vaginal bacterial flora between household and stray queens remains unknown. The aim of this study was to investigate the occurrence of vaginal and uterine bacteria in clinically healthy household and stray queens and to correlate culture findings with specific stages of the oestrous cycle. Vaginal and uterine samples from 40 clinically healthy queens were collected for isolation of bacteria and cytological examination. Bacteria were isolated from 31 vaginal swabs (77.5%) from stray (16/20; 80%) and household (15/20; 75%) cats. The isolates were more frequently detected in pure culture (18/31; 58%) than in mixed cultures (13/31; 41.9%). Streptococcus spp. was the most commonly identified bacteria (n = 16; 51.6%), followed by coagulase negative Staphylococcus spp. (n = 15; 48.4%) and E. coli (n = 12; 38.7%). A mixed bacterial culture of E. coli and Streptococcus spp. was commonly detected (50%), mainly in households (66.7%), whereas a mixed culture of Staphylococcus spp. and Streptococcus spp. (41.6%) was commonly isolated from stray cats (60%).  The frequency of isolation of pure or mixed bacterial cultures and the isolates did not vary significantly during the different stages of the oestrus cycle. All uterine samples tested were negative for bacteria. This study identified the most common bacteria in the vagina of clinically healthy cats. The isolation of vaginal bacteria in pure or mixed cultures should be considered as normal finding. The stage of the oestrous cycle apparently does not affect vaginal bacterial flora. Vaginal bacteria may differ between stray and households cats. No bacteria can be isolated from the uterus of clinically healthy cats.

This study aimed to assess the efficiency of ketamine and medetomidine by two different doses and routes on anesthesia depth and cardiac stability in red-eared sliders. Each turtle was anesthetized two times, with seven days wash-out period. Induction of anesthesia consisted of a bolus combination of ketamine (10 mg/kg) and medetomidine (0.2 mg/kg) administered in the left brachial biceps in the intramuscular protocol, or a bolus combination of ketamine (20 mg/kg) and medetomidine (0.2 mg/kg) administered in subcarapacial sinus after clear blood presence confirmation in the intravenous protocol. Vital signs, reaction on the skin palpation, manual mouth opening for endotracheal intubation, palpebral and cloacal reflex, and the withdrawal reflex of the front and hind limbs were measured and recorded every 5 minutes for 60 minutes after anesthesia injection. Atipamezole (1 mg/kg) was administered in the right brachial biceps one hour after ketamine and medetomidine administration. Needle insertion and possible painful reactions to drug administration were also evaluated and recorded. Obtained data were analyzed for normality and paired t-tests, Wilcoxon, or McNamar tests were performed where appropriate. The values of P ≤ 0.05 were considered significant. A significantly less pronounced decrease in heart rate was observed with intravenous anesthesia protocol. Both protocols recorded complete anesthesia recovery 60 minutes after intramuscular atipamezole administration. A ketamine-medetomidine dose combination administered intravenously provides a more stable and consistent anesthetic plane in red-eared sliders than ketamine-medetomidine administered intramuscularly.

A case of umbilical hernia in a 7-month-old female rabbit was presented to the Surgery Clinic of the Veterinary Faculty, University of Sarajevo. The owner noticed inappetence and lethargy four days before arrival at the clinic. Clinical parameters on physical examination were within the physiological range. Bruxism and lethargy were noted as signs of discomfort due to gas accumulation in colon. A reponible, nonpainful mass in the umbilical scar area was palpated but intestinal peristalsis was not altered. General anesthesia was induced by intramuscular administration of ketamine with medetomidine and maintained with isoflurane. Surgical treatment of hernia included the peritoneal sac dissection and amputation, repositioning of small intestines, and correction of abdominal wall defect. Intraoperative multimodal analgesia approach was used to reduce inhalant anesthesia requirements and to prevent pain-related and stress-related complications. In this case report we described a surgical and veterinary treatment of the reponible umbilical hernia in a rabbit.

This case report describes the remission of the fibrovascular corneal lesions in both eyes after bilateral superficial keratectomy combined with medical treatment in a German Shepherd dog. A completely blind German Shepherd dog diagnosed with chronic superficial keratitis (CSK, pannus) underwent bilateral superficial keratectomy along with subconjunctival administration of 0.1 mL of bevacizumab and mitomycin C, which where topically applied over the degenerated area of the cornea. Following the surgical procedure, cyclosporine topical ointment was given daily as life-long therapy. Notable improvements in behavior and physical activity were evident in the patient three to four weeks after the treatment. During a follow-up period over 24 months, complete remission of the fibrovascular corneal lesions was observed in both eyes, with minor seasonal conjunctival hyperemia successfully managed by administering dexamethasone and mitomycin C eye drops. This is the first report of using this specific combination of drugs during and after keratectomy in the dog, resulting in remission of CSK with no reported side effects. Superficial keratectomy, along with the administration of bevacizumab via subconjunctival injection, may present a novel approach for addressing superficial corneal neovascularization in dogs.

Over the past decades, recognised importance of prompt and valid pain recognition and quantification in veterinary medicine significantly increased interest in investigating attitudes and self-rated abilities of veterinarians towards pain assessment. However, giving that the owners are the ones who decide when to call the veterinarian, it is also essential to investigate their attitudes and knowledge regarding pain recognition and management. This is the first research investigating horse owners’ attitudes and self-rated abilities towards pain assessment and management in Bosnia and Herzegovina. The participants were invited via email or social media with attached link to online questionnaire created using the Google Forms platform. Surveyed population included adult sport or pleasure horse owners, and horse caretakers in Bosnia and Herzegovina. Study response rate was 33.33% (40 respondents). The questionnaire consisted of sections asking about respondents’ demographic data, general pain assessment and management, and attitudes towards pain assessment done by veterinarians compared with theirs. Study respondents considered their abilities for pain recognition and quantification as sufficient and sufficient/moderate, even though most of them were unfamiliar with pain scales, and only negligible number use them. This study indicates the need and importance of owners' education concerning pain recognition and quantification in horses, and their more effective communication with veterinarians.

The information regarding the risk of anesthesia-related death in veterinary medicine is scarce, and little is known about the mortality risk of specific anesthetics. The study conducted during 2019 at University of Sarajevo, Veterinary faculty, aimed to estimate the mortality risk of intermittent injectable ketamine-xylazine anesthesia in dogs and to investigate the potential relationship between mortality rate and anesthesiologists’ experience. Anesthetic records, where ketamine and xylazine combination was used for anesthesia induction and maintenance, were reviewed and divided into two groups: inexperienced (AN1) and experienced anesthesiologists (AN2). Inexperienced anesthesiologists were constantly supervised by experienced ones, whose corrective interventions were recorded. Overall detected mortality rate was 0.15%, with 0.18% and 0.11% in the AN1 and AN2 groups, respectively. A statistically significant difference was not found. Records of the AN1 group revealed interventions of experienced anesthesiologist in 92% of cases. Detected mortality rate was within the values previously established for inhalant anesthesia indicating high safety in usage of investigated protocol, if performed by experienced anesthesiologists. The high percentage of interventions of a senior anesthesiologist suggests that supervised upskilling of inexperienced anesthesiologists before their independent work could result in a better outcome.

Little is known about the presence of methicillin-resistant Staphylococcus aureus (MRSA) in a canary bird (Serinus canarius domesticus). Although MRSA in a canary bird was previously reported, to the best of our knowledge, this is the first full description of the isolation and antibiotic resistance pattern of MRSA in this pet bird. A swab was taken from the nonhealing wound on the lateral thigh of a four-year-old, caged, housed alone, male canary bird. After the identification of Staphylococcus aureus, the antibiotic susceptibility profile of the isolate was obtained by the disk diffusion test. According to the resistance to Oxacillin and Cefoxitin, the isolate was identified as MRSA. The mecA gene was confirmed by PCR. The bird was treated by offering drinking water medicated with an injectable enrofloxacin formulation at 200 mg/L over 10 days period. Two weeks after therapy, intensive contraction of the wound was observed with a reduction in size. A week later, the complete epithelization of the wound defect was verified. In this study, we could not confirm the source of infection in a canary bird, but we believe that transmission was from wild birds when the cage was putting out in order to allow the bird to sunbathe or more likely via contact with the owner. The results of this study underline the necessity for further investigations on the epidemiological role of canary birds as potential reservoirs of MRSA.

that the air sacs. The morphology of the air sacs system has been described in many domestic and wild bird Abstract | The research was conducted with the aim to investigate the morphology of air sacs system in Crimson Rosella ( Platycercus elegans ) parrots. Five adult birds, two males and three females were used in this study. The lungs and air sac system were injected via trachea with 26% solution of Vinylite mass. The obtained cast showed that these parrots have nine air sacs. The clavicular air sac was the only unpaired, while the cervical, cranial thoracic, caudal thoracic and abdominal air sacs were paired. The morphology of the air sacs was generally similar to that reported in other bird species, however, some specific features were identified. As most prominent among them were a partial fusion of the cervical air sacs, communication between the left and right subpectoral and perirenal diverticula and connection between the claviclar and cranial thoracic air sacs. The present investigation provided detailed and comprehensive data about the morphology of air sacs system in these parrot species and these findings will be very useful for future clinical examination and treatment of this birds.

There are only a few reported cases of abdominal ectopic pregnancy and consequential foetal mummification in dogs. The majority of the described cases indicate that this condition may exist for a prolonged period of time without any manifestation of illness. Therefore, sudden signs of abdominal distension, abdominal pain, episodes of epileptiform seizures, dark diarrhoea and lethargy, as were observed in the present case, are not likely to be considered as symptoms of an abdominal ectopic pregnancy. Furthermore, to the best of our knowledge, this is the first report of an eight-year long abdominal pregnancy in an ovariohysterectomised bitch. We present the clinical, laboratory, radiological and intraoperative findings, and the medical and surgical therapy for an abdominal ectopic pregnancy in a 14 year old Miniature Pinscher bitch, ovariohysterectomised eight years previously.

Abstract A ten-month-old male pet rabbit was referred to the Surgery Clinic of the University of Sarajevo Faculty of Veterinary Medicine with the history of several days gradual loss of appetite, decreased defecation frequency and the weight loss. Physical examination revealed low body condition score, tachypnea, tachycardia and distended abdomen painful on palpation. Abdominal radiographs were unremarkable displaying abdominal distention with gas accumulation in the intestines. Urgent explorative celiotomy was performed, and definitive diagnosis of intestinal volvulus was maded. Manual reposition of the intestines secured reestablishment of the intestinal blood flow, and after the observation of intestinal peristalsis the abdomen was closed in a routine fashion. Analgesics, antibiotics and supportive therapy were administered over the next seven days, and the case was finally concluded 14 days post-surgery without any complications detected. To the best of our knowledge, this is the first report of spontaneous intestinal volvulus in a pet rabbit diagnosed by exploratory laparotomy and successfully surgically resolved. Keywords: gastrointestinal stasis, distended abdomen, gas accumulation, surgical therapy

The objective of this study was to evaluate whether the addition of morphine or tramadol to lidocaine influences serum lidocaine concentrations in dogs administered these drugs epidurally prior to elective orchiectomy or ovariohysterectomy. Thirty dogs were divided into three groups of 10 each, with equal numbers of females and males. One group received epidurally 2% lidocaine combined with 0.9% NaCl, another received 2% lidocaine combined with morphine, and a third group received 2% lidocaine combined with tramadol. Blood samples for the determination of serum lidocaine concentrations were taken 10, 30, 60 and 90 min after epidural administration. Serum lidocaine concentrations were measured by gas chromatography-mass spectrometry (GC-MS). There was a total of 120 serum samples obtained. In most animals a peak serum lidocaine concentration was detected ten minutes after epidural administration. There were no significant differences in the detected serum lidocaine concentrations within observed groups of animals, or between males and females. Therefore, results suggest that morphine and tramadol can be used as additives to epidural lidocaine in dogs at the investigated doses, without significantly influencing lidocaine absorption from the epidural space and its serum concentration.

Equine pastern dermatitis (EPD), also known as grease, scratches or mud fever, represents variety of skin inflammatory conditions of the pastern region, all classified under this term. Therefore, it is rather considered a specific syndrome than a single disease diagnosis, with three main forms described. EPD has multiple potential etiologies (parasite (mite) infestation, bacterial infection, dermatophytosis, photosensitization, vasculitis, vaccinia, pemphigus foliaceus and primary irritant contact dermatitis), which are usually preconditioned with poor environmental conditions, particularly increased humidity and unappropriated hygiene. EPD has never been formally investigated in any area of Bosnia and Herzegovina. This is a preliminary investigation of the presence of EPD in the population of randomly selected horses in BiH. The research iincluded 40 horses of both genders and varied breeds and ages. EPD was detected in 12.5% of the examined horses with dominant presence in Arabian horses. Keywords: horse, equine pastern dermatitis, mud fever