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Selma Zukić

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Selma Zukić, Edin Bujak, Edita Vučić

Previous research on archaeological skeletal remains in Bosnia and Herzegovina rarely included odontological analysis. The objective of this research was biological profiling through odontological analysis (dental morphological characteristics, estimation of sex and dental age at death) in medieval archaeological skeletal remains samples from Krešića greblje in Paoča (Čitluk municipality, Bosnia and Herzegovina). After the excavations of the archaeological site, odontological analysis was performed before genetic analysis. The sample comprised 22 permanent teeth from 6 individuals. Odontological analysis included odontometric estimation of sex, estimation of age at death by two morphological methods, and an analysis of dental traits and dental pathology. Genetic analysis was used for sex determination. The results of odontological analysis classified all individuals as adults, with estimated age at death 20-25 in one individual, 30-35 in three individuals, 35-45 in one individual, and one individual older than 45 years of age. Odontometric sex estimation was in high concordance with sex determination by genetic analysis, and correctly classified five individuals’ sex (four females and one male). In one individual odontometric sex was assessed as female while genetic analysis revealed male. Morphological dental traits and dental pathology were investigated as well, adding to a better understanding of the odontological characteristics of the medieval population in the area of Bosnia and Herzegovina. Sex and dental age estimation given by odontological analysis provide fast and cost-effective information, and should be performed as an auxiliary before genetic analysis.

Background/Aim:Dental age estimation in adults is a challenging process due to the lack of sufficient information on the accuracy of dental methods applied in the identification of persons without information on identity. Previous studies on dental age estimation in adults suggested that each method should be tested on a population other than one which was used to develop the specific method. The aim of this study was to estimate dental age in adults from Bosnia and Herzegovina with the radiographic analysis of the pulp/tooth ratio of lower canines and to determine differences from chronological age. Material and Methods: The sample for the research comprised 50 periapical radiographs, with visible intact lower canine teeth, derived from an archive of the Department of Dental Morphology, Dental Anthropology and Forensics. After the selection process, all radiographs were digitalized. Additional information used is the chronological age and sex of the person to whom the images belonged. The measurements of the pulp space and the lower canine tooth were performed using the ImageJ computer methods. The results of measurements were entered in formulas for assessments of dental age, as per equations given by the authors. Results: The research has shown that there is a significant difference in examined population between the dental age estimated by the analysis of pulp/tooth ratio of lower canine teeth and the chronological age. The standard error of regression for Cameriere model was 14.12 years, and this model proved to be statistically significant (p= 0.0017). Kvaal-Solheim model from 1994 has shown a standard error of 15.07 years, and did not prove to be statistically significant (p= 0.054), while another model from 1995, by the same authors, shows a standard error of 14.64 years, and it proved to be statistically significant (p= 0.011). Conclusions: It is possible to estimate dental age by means of pulp/tooth ratio. However, it is necessary to conduct further research that will include a larger number of examinees and different age groups and also consider the specificity of teeth in Bosnia - Herzegovina's population to acquire more accurate data on accuracy and reliability of those methods in this population.

Introduction: Dental fear and anxiety (DFA) is a ubiquitous entity among dental patients in terms of their prevalence and incidence. It is among the major clinical problems in dentistry. In addition, the differences in DFA prevalence were present considering the age and gender of patients and over time, but with some opposite reports. The aim of this study is to examine the prevalence of DFA presence in children concerning their age, gender, and over time. Methods: The survey sample comprised 200 of 9–12-year-old children. The DFA presence was determined twice by the modified version of the CFSS-DS scale (CFSS-DS-mod scale) during a 6-months long period between the first and the subsequent dental appointment due to the need for restorative dental treatment. The scale was applied before the restorative treatment started on both occasions. Results: The prevalence of DFA was 17.5% in the study sample and decreased over time. It was slightly higher in girls. Conclusions: The DFA prevalence in 9–12-year-old children is decreasing over time. Latent manifestations of DFA presence should be considered for evaluation in the future.

Objectives Oral health in children is additionally burdened with the presence of dental fear and anxiety (DFA). These clinical psychologic entities in their progressive stages inevitably lead to avoidance of dental appointments, which makes prevention and therapy of oral diseases more difficult. Upon the onset of DFA in general, as one of the emotional outcomes of stress in a dental office, most children patients could define the specific stressors that were most intense for them, which could predict the presence of DFA. Aim To examine the predictors of DFA presence in 9-12-year-old children, and investigate how they could explain the DFA occurrence in study participants. Material and Methods The sample consisted of 200 children aged from 9 to 12 years. The DFA presence was determined by the modified version of the CFSS-DS scale. The child's behavior in the dental office during the treatment was evaluated by the trained observer using Venham anxiety and behavior rating scales. Socioeconomic status, characteristics of dental office visits, and previous caries experience were also analyzed. Results The main DFA predictors were related to invasive dental treatments, where the behavior during dental treatment was the most accurate expression of the DFA appearance. Conclusions Invasive dental procedures are the main stressful factor for DFA occurrence. Predisposing factors could strengthen the DFA occurrence.

Belma Jusić, M. Dzehverovic, A. Pilav, Samra Terzić, Selma Zukić, Edin Bujak, J. Cakar

During 2019, 28 grave constructions were excavated at cemetery Metaljica (Hadzici municipality, Bosnia and Herzegovina). Archaeological excavation have revealed 17 well preserved skeletons that were subjected to anthropological, odontological and genetic analyses. To determine sex by genetic analysis, amelogenin and DYS391 loci were taken into consideration. Concordance between results of anthropological, odontological and genetic analyses applied in this research occurred in one sample out of 17. In four samples, sex was determined only by genetic analysis, since sexual dimorphism indicators were not preserved. Concordance between odontological and genetic determinations was observed in two samples, while affirmative results of anthropological and genetic analysis were obtained in only one sample. Discrepancy in the majority of samples can be atributted to the state of preservation of skeletal remains, interpopulation differences and allele drop-out. Moreover, anthropological and odontological analyses were not applicable to juvenile/subadult skeletons, since sexually dimorphic characteristics relevant for metric and morphological analysis are not developed well at that developmental period. This study emphasizes the importance of combining anthropological, odontological and genetic methods in order to determine sex of archaeological skeletal remains accurately.

Belma Jusić, M. Dzehverovic, A. Pilav, Samra Terzić, Selma Zukić, Edin Bujak, Jasmina, Cakar

1Laboratory for Forensic Genetics, University of Sarajevo-Institute for Genetic Engineering and Biotechnology, Bosnia and Herzegovina 2Department of Archaeology, Faculty of Humanities and Social Sciences, University of Zagreb, Croatia 3Department of Dental Morphology, Dental Anthropology and Forensics, University of Sarajevo-Faculty of Dentistry with Clinics, Bosnia and Herzegovina 4Department of Archeology, Faculty of Philosophy, University of Sarajevo, Bosnia and Herzegovina

Alma Gavranović-Glamoč, Lejla Kazazić, Sanela Strujić-Porović, E. Berhamović, Amela Džonlagić, Selma Zukić, Selma Jakupović, Selma Tosum Pošković

Introduction: Perceptions of the esthetic appearance of teeth vary in different individuals and can affect satisfaction with the dental appearance, attitudes, and the need for appropriate dental treatment. The research aims to examine the factors influencing the satisfaction with the appearance of the dentition and the attitude toward treatments which improve dental esthetics among students of the faculty of dentistry in comparison to the attitudes of students of non-dental faculties.Methods: The research included a total of 358 students of the Faculty of Dentistry and Faculty of Architecture in Sarajevo who voluntarily filled out a questionnaire created for this research. The questionnaire contained questions related to satisfaction with the appearance of teeth in general, tooth color, tooth position, questions related to the previous, and future desired esthetic restorations and treatments.Results: Female subjects expressed statistically significantly greater dissatisfaction with the appearance of the dentition and did or plan to do treatments that could improve dental esthetics compared to male subjects. Students of the Faculty of Dentistry at final years were significantly more satisfied with the general appearance of teeth and tooth color compared to freshmen students of the Faculty of Dentistry and students of the Faculty of Architecture.Conclusion: Satisfaction with dental appearance is a subjective experience that is influenced by various factors, level, and type of education is among them.

Alma Gavranović-Glamoč, Muhamed Ajanović, Lejla Kazazić, Sanela Strujić-Porović, Selma Zukić, Selma Jakupović, Alma Kamber-Ćesir, Lejla Berhamović

OBJECTIVE To evaluate and compare the solubility of three luting cements in three different solutions: distilled water and artificial saliva with different pH values (7.4 and 3.0). MATERIALS AND METHODS Resin-modified glass-ionomer cement (GC Fuji Plus) and two resin cements (Multilink Automix and Variolink II) were used. A total of 45 specimens, 15 specimens (15x1 mm) for each cement, were prepared according to ISO standard 4049:2009. The solubility of the cements was calculated by weighing the specimens before and after immersion and desiccation. Values of solubility in water (Wsl) in microgram/mm3 for each of the five specimens were calculated using the following formula (ISO 4049:2009): Wsl=(m1-m3)/V. The Mann-Whitney U nonparametric statistical method and Post hoc sample comparisons were applied. RESULTS GC Fuji Plus showed statistically significant higher solubility in comparison with Variolink II and Multilink Automix in all three solutions. In acidic artificial saliva (pH 3.0) Multilink Automix showed significantly higher values of solubility compared to Variolink II (P<0.016). By studying the effect of pH value on the solubility of GC Fuji Plus cement, significantly higher values of solubility in pH 3.0 artificial saliva were confirmed (P<0.009). The influence of the surrounding pH value on the solubility of the resin cements Multilink Automix and Variolink II was researched. No statistically significant difference was found. CONCLUSION Solubility values were mainly influenced by the proportion of hydrophilic matrix, the type and composition of filler, and the pH value of the solutions.

Amra Ahmić Vuković, Selma Jakupović, Selma Zukić, Anita Bajsman, Alma Gavranović Glamoč, S. Šečić

OBJECTIVE The aim of this paper was to analyze the distribution of stress and deformation on the mandibular first premolar under two types of loading (axial and para-axial load of 200 N) using the FEM computer method. MATERIALS AND METHOD For this research a µCT scan of the first mandibular premolar was used, and the method used in this research was FEM analysis under two types of loading. RESULTS The values of the von Mises stress measured in the cervical part of an intact tooth under axial load were up to 12 MPa, and under paraaxial load over 50 MPa. The values of the stress measured on the bottom of the noncarious lesion are very high ≈ 240 Mpa. Stress values in the cervical part of the intact tooth are higher in the zone of the sub-surface enamel. The deformation values of the tooth under para-axial loading were ≈ 10 times higher than the value of the deformation under axial load. The greatest deformations were seen in the area of the tooth crown. CONCLUSIONS Occlusal loading leads to significant stress in the cervical part of teeth. The values of the measured stress are greater under the action of paraxial load. The values of stress in abfraction lesions measured under a paraxial load are extremely high. Exposing the lesion to further stress will lead to its deepening. The total deformation of the entire tooth under paraxial load was ≈ 10 times higher compared to the deformation value of the tooth under axial load.

Jasmina Mlaćo Durek, A. Vuković, Selma Zukić, A. Tiro, Anita Bajsman, Nermana Kurić

Aim of this study was to examine the existence of  sexual dimorphism of the first permanent maxillary molars in the population of central Bosnia. The sample for the study comprised 94 subjects with intact crowns of the first permanent maxillary molars, without orthodontic anomalies. Mesiodistal and buccopalatal diameters were measured on the plaster models, based on calculated variables: robustness index or crown area (IRC) and sexual dimorphism (SD). Linear dimensions of the first permanent maxillary molars, as well as the IRC exhibited sexual dimorphism with larger values in males, except MD diameter on the left side. The greatest sexual dimorphism demonstrates IRC from the right. Linear dimensions of the first permanent maxillary molars and IRC can be used for sex determination in population of Central Bosnia.

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