Nigella sativa is a herb that has been used for centuries to treat various ailments, including infectious diseases. The aim of this work was to show in laboratory conditions the influence of different temperature regimes and methods of storage of Nigella Sativa oils on their physicochemical properties and microbiological stability. Analyzes have established that the temperature and method of storage have a significant influence on the change in the quality of the oil, especially when it comes to the total acidity of the oil and the peroxide value, where the values increased with the time of storage. The lowest obtained value for the peroxide number was at the first measurement, Pb = 9.99, which meets the prescribed standards for safe use, then the highest obtained value was 34.47 for the sample that was constantly exposed to the sun in a bright bottle. Also, the values of the iodine number increased with time, where the lowest value obtained was 14.56, and the highest was 102.48. Therefore, it is extremely important that the oil is stored in suitable packaging in dark glass bottles, and that it is protected from direct sunlight and high temperatures.

New accurate, precise, and sensitive spectrophotometric method were developed for the assay of L-ascorbic acid in pharmaceutical preparations. The determination of L-ascorbic acid was based on its oxidation by potassium peroxydisulfate in the presence of Ag(I) as a catalyst. The molar absorptivity of the proposed method was found to be 8.61 · 103 L mol-1 cm-1 at 248 nm. Beer's law was obeyed in the concentration range of 0.46–20.0 μg mL–1. Other compounds commonly found in vitamin C and multivitamin products did not interfere with the determination of L-ascorbic acid. The proposed method was successfully applied for the determination of L-ascorbic acid in pharmaceutical formulations. The results obtained with the proposed method showed good agreement with those given by the titrimetric method using iodine.

Physical chemical milk is an emulsion of milk fat in an aqueous solution of proteins, milk sugar and mineral salts. The high molar conductivity of goat milk samples compared to cow's milk indicates a high content of mineral substances. That goat milk is rich in total proteins is also indicated by the protein content in the samples, which are higher than the cow's milk samples. However, higher fat content was recorded in cow's milk samples, which also results in higher surface tension of cow's milk. The freezing point and refractive index of goat milk are higher compared to literature data and cow milk samples. The acidity of goat's milk comes from the acidic properties of casein, citrate, phosphate, etc. it is lower than cow's milk and is in accordance with literature data. The viscosity of pasteurized goat's milk at all temperatures is also higher than that of cow's milk.

Waste water in the galvanic process contains high concentrations of heavy metals that pose a direct danger to humans and the environment. Conventional methods for their removal are quite expensive and generate a large amount of waste. The development of new and improvement of existing methods for the removal of heavy metals from galvanic wastewater are the subject of many studies. Compared to other purification methods, the adsorption is becoming an increasingly popular method of wastewater purification, especially if the adsorbent is cheap, easily available and does not require any other treatment before use. Therefore, the aim of the work was to investigate the possibility of using natural bentonite for the removal of heavy metal ions from multi-component water systems of the galvanic industry. For this purpose, the physico-chemical characterization of natural bentonite was performed, and then the influence of pH value, time and temperature on the adsorption efficiency was examined. The results of adsorption showed that natural bentonite can be used as an adsorbent for the removal of heavy metal ions from waste galvanic waters, and that at pH 5 it achieves the maximum removal efficiency for Cu(II):Cr(III):Ni(II) ions in the percentage ratio 100 : 99.990 : 99.998. The results showed that the highest removal efficiency for Cu (II) ions was achieved in the first 10 minutes, and 20 minutes for Cr (III) and Ni (II) ions. The maximum efficiency of Cu (II) removal was achieved at all temperatures, while for Cr (III) 99.99% and Ni (II) 100% maximum efficiency was achieved at 35°C, which indicates that the adsorption process is endothermic. The experimental results of the adsorption of Cu (II) metal ions are in good agreement with the Langmuir and Freundlich theoretical models, while for Cr (III) and Ni (II) ions they are in better agreement with the Langmuir adsorption model.

Medicinal plants have become an increasing subject of interest worldwide due to the large amount of biologically active substances that have potential beneficial health properties. One of the more interesting popular medicinal plants is hawthorn (Crataegusspp.), a deciduous branched shrub that is increasingly used for health purposes. Various parts of this plant, including berries, flowers and leaves, are rich in nutrients and beneficial bioactive compounds that are effective in the treatment of numerous diseases. Honey is a natural sweetener produced by bees from plant secretions. Known for its nutritional and medicinal values, it gives strength to the body, which is why it is indispensable in the human diet. The combination of these two ingredients represents a significant source of vitamins and minerals in daily use, but also for use in various pharmaceutical and medical purpose. Taking into account all of the above, the aim of the work is to test samples of the mixture of honey and hawthorn in different proportions and determine the physical and chemical characteristics: pH value, electrical conductivity, refractive index, viscosity, water activity, HMF, DPPH. Based on the analysis, appropriate conclusions will be drawn and more information will be obtained about their quality and possible use as a food supplement. On the basis of the conducted analyses, it can be concluded that the parameter values are within the permitted limits defined by the Rulebook (Official Gazette Bosnia and Herzegovina No. 37/09). The analysis of the mentioned parameters showed that the chemical composition of the sample plays a major role in the value of the measurement results, and that Sample III has the best antioxidant properties. KEYWORDS:physicochemical characteristics; honey; hawthorn (Crataegus spp.);mixture; food suplement

Various synthetic and natural antioxidants are used to reduce oxidation and its negative impact on the oil during the food frying process. Considering that some studies show the negative impact of synthetic antioxidants on the health of consumers, natural alternatives are being used more and more. BHA and BHT are synthetic antioxidants that are widely used in the food industryand a large number of natural compounds such as phenols, anthocyanins, flavonoids, vitamins, etc. show antioxidant properties. In this study, the antioxidant capacity of oregano and rosemary essential oil was tested and compared with the antioxidant capacity of BHA and BHT. The antioxidant capacity was evaluated by the removal of DPPH radicals and by iron reduction (FRAP). The results of this study showed that the studied essential oils exhibited antioxidant capacity. These oils have a high antioxidant capacity, however, compared to synthetic antioxidants, they show a significantly lower antioxidant capacity but they can be used as natural antioxidants during food processing. KEYWORDS:antioxidant capacity; essential oils; synthetic antioxidants; lipidoxidation

Using natural and modified Ca-bentonite as an adsorbent to observe a satisfactory trend in the removal of heavy metal ions As(V) and Hg(II) from simulated wastewaters. In this original scientific paper, Ca-bentonite was modified in two ways, thermally activated at a temperature of 300 °C for 3 hours and acid activated with HCl and H2SO4, molar concentrations 0,4 mol/L. Ca-bentonite used in this original scientific work was used from the Shipovo mine (Šipovo mine), Bosnia and Herzegovina and proved to be an excellent bioadsorbent for the removal of present ions from simulated wastewaters. Also this is eco-friendly adsorbent and low costed compared to other expensive adsorbents. Due to its chemical composition in which two oxides predominate, namely SiO2 (48.28 mass %) and Al2O3 (23.04 mass%), it can be concluded that Ca-bentonite from the Shipovo mine (Šipovo mine), Bosnia and Herzegovina belongs to the group of refractory materials. The highest removal efficiency of As(V) ions expressed in % was 75.11 at the initial concentration of 1.5 mg/L and was recorded using HCl acid-activated Ca-bentonite. The efficiency of Hg(II) ion removal was the highest using thermally activated Ca-bentonite and this value was 99.66% at an initial concentration of 1 mg/L.

pH represents the concentration of free H+ in pine needles extracts (PNE) and is therefore an important initial parameter in quality control. Electrical conductivity and pH of samples of fresh and stored for 20 days of PNE with black cumin oil and olive oil had values of 0.00 due to the encapsulation of water molecules, pH and electrical conduction was not possible. The pH of the other samples was in a weakly acidic environment because the pH of natural pine needles is 3.8. Electrical conductivity values in all samples except pine needle extract and honey increased during storage. By monitoring the parameters of pH and electrical conductivity in the quality control of PNE, it gives us a significant insight into the physical state of the phases and the way of storage.

The aim of this study was to examine the content of total soluble substance (TSS), total acidity (TA), pH, ascorbic acid content (vitamin C) and total phenols in fresh strawberries, as well as in strawberries stored for 10 days by freezing. Chemical characterization of fresh and frozen strawberries was done on four different varieties: Clery, Maya, Asia, Joly grown in the area of Čelić, Bosnia and Herzegovina. The obtained results showed that frozen strawberries have slightly lower values of TSS, pH, vitamin C, while the content of TA and total phenols was slightly higher in frozen strawberries than in fresh ones, which can be attributed to increasing acidity.