The data on the relationship on gastroesophageal reflux (GER) and brief resolved unexplained events (BRUE) in infants are scarce. The aim of this study was to investigate the diagnostic usefulness of combined multichannel intraluminal impedance-pH (MII-pH) monitoring in children with suspected GER-related BRUE. Infants hospitalized due to BRUE and who were referred for 24-hour MII-pH monitoring from January 2012 to September 2017 were prospectively included in the study. All children underwent 24-hour MII-pH monitoring using a MIIpH ambulatory system (Ohmega, MMS, Enschede, Netherlands). The impedance recordings were analyzed using criteria described in a consensus statement on indications, methodology and interpretation of combined MII-pH monitoring in children. Twenty-one infants (mean age 4.7 months; range 0.9 to 8.9 months; male/female 10/11) participated in the study. Irregular breathing/apnea associated with GER was found in 10 (52.4%) of infants. Based on RI on pH-metry alone only 7 (33.3%) infants would be diagnosed with GERD. More than 100 GER episodes detected by MII were found in 10 (47.6%) infants. Nineteen percent of infants had GERD diagnosed based on both pH-metry and MII. Acid and non-acid reflux seems to have a significant role in the pathogenesis of GER-related BRUE.

Introduction: This research aimed to determine the impact of horseback riding on the balance ability of first-grade students. Material and methods: Fifty-eight students were divided into two sub-groups: twenty-eight riders and thirty non-riders. The subtest of balance is composed of nine items which were grouped into five variables: Total balance, Static balance, Dynamic balance, and Open and Closed eyes balance. The Mann-Whitney U test was used to determine the significance of the difference between the groups, and the strength of the effect of intergroup differences was determined using the Cohen criteria. Results: The results showed that there were statistically significant differences between groups in Total balance, Static, and Balance with closed eyes. Cohen's criterion indicates that there was a strong effect of intergroup differences in performance demanding balance tasks of 7-year-old children. Conclusion: It can be observed that horseback riding has the effect of reducing the difference between the maximum and minimum balance points by enhancing competencies at the minimum level.

In this randomized controlled pilot trial, we investigated the effects of a 6-month intake of hydrogen-rich water (HRW) on several molecular and phenotypic biomarkers of aging in older adults aged 70 years and over. Forty older adults (20 women) were randomly allocated in a parallel-group design to receive 0.5 L per day of HRW (15 ppm of hydrogen) or control drink (0 ppm of hydrogen) during a 6-month intervention period. The biomarkers assessed at baseline and 6-month follow up were molecular markers in the blood (DNA and chromosomes, nutrient sensing, protein, and lipid metabolism, oxidative stress and mitochondria, cell senescence, inflammation), brain metabolism, cognitive functioning, physical function and body composition, resting blood pressure, facial skin features, sleep outcomes, and health-related quality of life. The mean age, weight, and height of study participants were 76.0 ± 5.6 years, 78.2 ± 16.1 kg, height 167.5 ± 11.5 cm, respectively. A significant treatment vs. time interaction was found for telomere length (P = 0.049), with the length increased after HRW intervention (from 0.99 ± 0.15 at baseline to 1.02 ± 0.26 at follow up) and decreased after drinking control water (from 0.92 ± 0.27 to 0.79 ± 0.15). A marker of DNA methylation (Tet methylcytosine dioxygenase 2, TET2) expression at 6-month follow-up increased in both groups, yet the degree of elevation was significantly higher in HRW (from 0.81 ± 0.52 at baseline to 1.62 ± 0.66 at follow up) comparing to the control water (from 1.13 ± 0.82 to 1.76 ± 0.87) (P = 0.040). A strong trend for treatment vs. time interaction was found for a degree of DNA methylation (P = 0.166), with the methylation increased in the HRW group (from 120.6 ± 39.8 ng at baseline to 126.6 ± 33.8 ng at follow up) and decreased after taking control water (from 133.6 ± 52.9 ng to 121.2 ± 38.4 ng). HRW was superior to control water to increase brain choline and NAA levels in the left frontal grey matter, brain creatine at the right parietal white matter, and brain NAA at the right parietal mesial grey matter (P < 0.05). No significant differences were found between interventions for other outcomes (P > 0.05), except for a significantly improved chair stand performance after HRW intervention compared to the control water (P = 0.01). Owing to pleiotropic mechanisms of hydrogen action, this simple biomedical gas could be recognized as a possible anti-aging agent that tackles several hallmarks of aging, including loss of function and telomere length shortening. The study was registered at ClinicalTrials.gov (NCT04430803).

Aim of study: This research aims to determine if there are statistically significant differences among provenances of silver fir (Abies alba Mill.) as one of the most important tree species in Bosnia and Herzegovina, to choose a provenance with the best productivity for further silvicultural activities of silver fir in the country. Material and methods: The height and diameter at breast height were measured, and the basal area and volume of silver fir trees were calculated in the silver fir provenance test. The test contains nine provenances from Bosnia and Hezegovina and was launched in 1991, with 5-year-old seedlings (3+2). The data were collected in 2013. Main results: Variance analysis for all traits showed statistically significant differences among provenances. Average breast height diameter in 27-year-old plants for all provenances amounted to 11.0 cm, average height 8.4 m, average basal area 0.010342 m, and average volume 0.070845 m Highlights: The research results confirmed the existence of variability among silver fir provenances in Bosnia and Herzegovina, considering the morphological indicators. Provenance Bosanski Petrovac showed the highest values of all traits, which implies it is the best provenance for the further activities on seed collecting and producing planting material for afforestation in Bosnia and Herzegovina.

To shift the paradigm towards Industry 4.0, maritime domain aims to utilize shared situational awareness (SSA) amongst vessels. SSA entails sharing various heterogeneous information, depending on the context and use case at hand, and no single wireless technology is equally suitable for all uses. Moreover, different vessels are equipped with different hardware and have different communication capabilities, as well as communication needs. To enable SSA regardless of the vessel’s communication capabilities and context, we propose a multimodal network architecture that utilizes all of the network interfaces on a vessel, including multiple IEEE 802.11 interfaces, and automatically bootstraps the communication transparently to the applications, making the entire communication system environment-aware, service-driven, and technology-agnostic. This paper presents the design, implementation, and evaluation of the proposed network architecture which introduces virtually no additional delays as compared to the Linux communication stack, automates communication bootstrapping, and uses a novel application-network integration concept that enables application-aware networks, as well as network-aware applications. The evaluation was conducted for several IEEE 802.11 flavors. Although inspired by SSA for vessels, the proposed architecture incorporates several concepts applicable in other domains. It is modular enough to support existing, as well as emerging communication technologies.

The cyclotide T20K inhibits the proliferation of human immune cells and is currently in clinical trials for multiple sclerosis. Here, we provide novel functional data and mechanistic insights into structure–activity relationships of T20K. Analogs with partial or complete reduction of the cystine knot had loss of function in proliferation experiments. Similarly, an acyclic analog of T20K was inactive in lymphocyte bioassays. The lack of activity of non-native peptide analogs appears to be associated with the ability of cyclotides to interact with and penetrate cell membranes, since cellular uptake studies demonstrated fast fractional transfer only of the native peptide into the cytosol of human immune cells. Therefore, structural differences between cyclic and linear native folded peptides were investigated by NMR to elucidate structure–activity relationships. Acyclic T20K had a less rigid backbone and considerable structural changes in loops 1 and 6 compared to the native cyclic T20K, supporting the idea that the cyclic cystine knot motif is a unique bioactive scaffold. This study provides evidence that this structural motif in cyclotides governs bioactivity, interactions with and transport across biological membranes, and the structural integrity of these peptides. These observations could be useful to understand the structure–activity of other cystine knot proteins due to the structural conservation of the cystine knot motif across evolution and to provide guidance for the design of novel cyclic cysteine-stabilized molecules.

In this paper, we solve the problem of learning a generalized Nash equilibrium (GNE) in merely monotone games. First, we propose a novel continuous semi-decentralized solution algorithm without projections that uses first-order information to compute a GNE with a central coordinator. As the second main contribution, we design a gain adaptation scheme for the previous algorithm in order to alleviate the problem of improper scaling of the cost functions versus the constraints. Third, we propose a data-driven variant of the former algorithm, where each agent estimates their individual pseudogradient via zeroth-order information, namely, measurements of their individual cost function values. Finally, we apply our method to a perturbation amplitude optimization problem in oil extraction engineering.

Parkinson’s disease (PD) causes a progressive decline of motor and cognitive functions, often affecting postural control. Training through virtual reality has been shown to be effective in improving this condition. This study aims to analyse the effects of the Kinect Adventures! games in postural control of people with PD. Ten individuals diagnosed with idiopathic PD, in stages I to III of the Hoehn & Yahr scale, aged between 48 and 73 years, were selected. Fourteen training sessions of one hour each, twice a week, were performed. Individuals were evaluated pre, post-intervention and 30 days after the last session of intervention by a force platform that measured the oscillation area (COP) and velocity (VOS) of the centre of pressure in ten different sensory conditions and the Limits of Stability (LOS). LOS showed a statistically significant increase immediately after the training the 14 sessions, as were observed and there were no significant changes in COP and VOS immediately after the intervention or 30 days after the end of training. The results of this study indicate that the training with Kinect Adventures! Games improve the postural control of people with PD, by increasing the LOS. Keywords: Parkinson disease; postural balance; virtual reality exposure therapy; video game