In oral and written communication there exist various linguistic tools by which one tells or asks their interlocuter to do something. In such cases, one most often uses the imperative, even though in different languages there are different ways to attain a goal, i.e., to realize the intention or request of the message sender. In Latin, there existed along with the imperative a mood called the subjunctive through which speakers could express commands or prohibitions, but also suggestions, desires, requests, counsel, or encouragement for the message receiver to complete a certain act. The content mentioned can be expressed using the present (e.g., You are shutting up now!) or the future tenses (e.g., You are going to the library tomorrow!), or with certain periphrastic forms (e.g., This job ought to be finished!). The form, then, does not always correspond with the anticipated content; that is, one type of content can be expressed using various forms. This paper is directed at the observation and analysis of the various forms and functions of commands used by Cicero in his epistles, as well as on their classification according to which of them prevail in individual groups of letters. Along with this, seeing as in some examples ambiguous forms of the imperative are found, whose function is conditioned due to various pragmatic parameters and contextual frameworks, the goal is to also to point out the importance of context and its elements, especially those of a socio-political, cultural or historical environment and the role of participants in the choice, usage, and interpretation of forms of the command.

Pyrophyllite was investigated in this study as a potential adsorbent for the removal of glyphosate, a common herbicide for the control of a wide variety of weeds. There are several publications in the literature that discuss how glyphosate interacts with clay minerals such as montmorillonite, kaolinite, and bentonite, but there is no information regarding the possibility of glyphosate removal from aqueous solution using pyrophyllite. In this study, the adsorption of glyphosate from water samples onto pyrophyllite, a mineral clay from the Parsovici, Konjic mine, was investigated. The ninhydrin reaction was used to spectrophotometrically quantify glyphosate. At 570 nm, the purple Ruhemman product's absorbance was determined. Adsorption capacity (qe), removal efficiency (R), and the partition coefficient are used to express the results (Kd). The outcomes demonstrated that distinct ionic species of glyphosate at different pHs cause the adsorption of glyphosate on pyrophyllite to be pH-dependent. The findings demonstrated that during different contact times, the basic medium can remove up to 75% of glyphosate. According to results from fitting isotherm models, the Freundlich model is slightly better fitted. According to the results of kinetic investigations, the pseudo-2nd order model is more suitable for this kind of adsorption.

ABSTRACT Woman’s representation in politics is an ongoing issue in Bosnia and Herzegovina and Republic of Croatia. Even though these states have sufficient laws and legal documents that regulate woman’s political participation, situation in theory is still devastating. In this paper, we gave an overview of mentioned legal framework in case studies after which we analyzed current status of woman’s representation in politics through primary method of literature review. Data was collected through primarily and secondary sources. Primarily sources were based on the qualitative research of previous work, meta-analysis and comprehensive literature reviews while secondary data was collected from official webpages from relevant election commissions from Bosnia and Herzegovina and Republic of Croatia, with emphasis on the mixed-methods approach and small-n comparison. We came to conclusion that both countries have strong legal framework for ensuring higher level of female participation but still, it’s revival in today’s political world is still missing. Finally, we connected results of elected females with Society 5.0 Key words: woman’s representation; political participation; Bosnia and Herzegovina; Republic of Croatia; Society 5.0 ABSTRAK Representasi perempuan dalam politik merupakan isu yang sedang berlangsung di Bosnia dan Herzegovina dan Republik Kroasia. Meskipun negara-negara ini memiliki undang-undang dan dokumen hukum yang memadai yang mengatur partisipasi politik perempuan, situasi secara teori masih sangat memprihatinkan. Dalam makalah ini, kami memberikan tinjauan tentang kerangka hukum tersebut dalam studi kasus, setelah itu kami menganalisis status representasi perempuan dalam politik saat ini melalui metode utama kajian pustaka. Data dikumpulkan melalui sumber primer dan sekunder. Sumber utama didasarkan pada penelitian kualitatif dari pekerjaan sebelumnya, meta-analisis dan tinjauan literatur yang komprehensif sementara data sekunder dikumpulkan dari halaman web resmi dari komisi pemilu yang relevan dari Bosnia dan Herzegovina dan Republik Kroasia, dengan penekanan pada pendekatan metode campuran dan kecil. -n perbandingan. Kami sampai pada kesimpulan bahwa kedua negara memiliki kerangka hukum yang kuat untuk memastikan tingkat partisipasi perempuan yang lebih tinggi, tetapi kebangkitannya di dunia politik saat ini masih belum ada. Terakhir, kami menghubungkan hasil perempuan terpilih dengan Society 5.0 Kata kunci: representasi perempuan; partisipasi politik; Bosnia dan Herzegovina; Republik Kroasia; Masyarakat 5.0

It is often difficult to be certain which genes underlie the effects seen in association studies. However, variants that disrupt the protein, such as predicted loss of function (pLoF) and missense variants, provide a shortcut to identify genes with a clear biological link to the phenotype of interest. Glycosylation is one of the most common post-translationalmodifications of proteins, and an important biomarker of both disease and its progression. Here, we utilised the power of genetic isolates, gene-based aggregation tests and intermediate phenotypes to assess the effect of rare (MAF<5%) pLoF and missense variants from whole exome sequencing on the N-glycome of plasma transferrin (N=1907) and immunoglobulin G (N=4912), and their effect on diseases. We identified significant gene-based associations for transferrin glycosylation at 5 genes (p<8.06x10-8) and for IgG glycan traits at 4 genes (p<1.19x10-7). Associations in three of these genes (FUT8, MGAT3 and RFXAP) are driven by multiple rare variants simultaneously contributing to protein glycosylation. Association at ST6GAL1, with a 300-fold up-drifted variant in the Orkney Islands, was detectable by a single-point exome-wide association analysis. Glycome-associated aggregate associations are located in genes already known to have a biological link to protein glycosylation (FUT6, FUT8 for transferrin; FUT8, MGAT3 and ST6GAL1 for IgG) but also in genes which have not been previously reported (e.g. RFXAP for IgG). To assess the potential impact of rare variants associated with glycosylation on other traits, we queried public repositories of gene-based tests, discovering a potential connection between transferrin glycosylation, MSR1, galectin-3, insulin-like growth factor 1 and diabetes. However, the exact mechanism behind these connections requires further elucidation.

From February 2023 (Volume 23, Issue 1), the title of the Bosnian Journal of Basic Medical Sciences will be changed to Biomolecules and Biomedicine. The new title reflects the increasing number of published research done on subcellular/molecular level as well as translational and clinical research contained in the term Biomedicine. Biomolecules and Biomedicine will continue to be published by the Association of Basic Medical Sciences of the Federation of Bosnia and Herzegovina. Read more in the PDF.

Adil Zulfikarpašić, Put u Foču, Sarajevo: Bošnjački institut – Fondacija Adila Zulfikarpašića, 2022. 140 str., ISBN 978-9958-743-43-6.

The present research was undertaken to investigate polyphenolic profiles of peel, pulp and juices made from two standard commercial and five traditional apple cultivars from Bosnia and Herzegovina. The main goal of the study was to monitor the distribution and changes of polyphenolic profiles through different phases of apples’ processing into cloudy and clear juices, with regard to L-ascorbic acid pretreatment. Quantitative determination of phenolic compounds was carried out by using high-performance liquid chromatography with diode-array detection. The obtained results showed that traditional cultivars, namely ‘Paradija’ and ‘Prijedorska zelenika’, displayed significantly higher content of these compounds compared to commercial ones. Flavan 3-ols and flavonol glycosides were mostly found in peels of all cultivars (21.2–44.1 and 5.40–33.3%, respectively), while phenolic acids along with flavan 3-ols were predominant in the pulp (8.20–30.8 and 5.10–13.9%, respectively). Apples’ processing into juices caused decrease (more than 90%) in the content of all polyphenols and the distribution of these compounds from fruits to final products had a negative trend, particularly evident in clear juices. The most drastic loss occurred in the flavonol glycosides and dihydrochalcones content, while chlorogenic acid displayed quite stable distribution from apples to final products due to its good solubility. Apple mash pretreatment with L-ascorbic acid had a positive impact on the preservation and retention of polyphenols.

Introduction: information and communication technology (ICT) has multiplied its availability and use, resulting in enormous usage, especially among school-age children, who use it for an average of 7.5 hours per day. The amount of time adolescents spend in front of electronic screens has increased significantly between the ages of 10 and 14, but there are significant gender changes. For boys, this increase is 41.6 minutes per day, while for girls it is 22.7 minutes per day. Methods: The study is designed as an epidemiological, cross-sectional, descriptive-analytic, comparative study. The study will be conducted in the form of an online questionnaire on the Microsoft Forums platform during the period 12/15/2021 to 12/30/2021. Respondents were able to access the request for consent using a link and a QR code, which was previously signed by a parent/guardian giving consent for children to participate in this study. Results: The results of this study show that school-aged children generally use cell phones with a touch screen between 2 and 3 hours daily during the work week (39.2%) and TV (39.5%). Pain caused by ICT use in the past 12 months occurred mainly in the neck/shoulder area (42.4%), while pain in the previous month occurred mainly in the lower extremities (29.1%). Conclusion: Increased use of ICT devices was associated with higher levels of musculoskeletal symptoms. The higher pain burden in various body parts is of concern, and further research on the effects of ICT device use in adolescents is needed and warranted.

In der Gesamtbehandlungsplanung nimmt die Medikation unter spezifischen Gesichtspunkten eine spezielle Bedeutung ein. Individuelle, innerpsychisch-libidoökonomische, objektbeziehungstheoretische Konzepte werden entlang des Konzepts der „Resomatisierung“, des Handlungsdialogs, des Übergangsphänomens diskutiert. Krankheitsspezifische Bedeutungen bekommen anhand der Fallvignette Raum. In overall treatment planning, medication gains a special significance from specific points of view. Individual, inner-psychic, libido-economic, object relation-theoretical concepts are discussed along the concept of “re-somatization,” the action dialogue, and the transition phenomena. Disease-specific meanings are given space based on the case vignette.

Mogućnost umrežavanja makromolekula i konformaciona promenljivost lanaca spadaju u bazna svojstva, ne samo polimerne strukture, već molekulskog sveta uopšte. Elastične mreže su dugo privlačile pažnju relativno uskih krugova inženjera koji su se bavili proučavanjem proizvoda od gume. Savremeno dizajniranje bio-kompozitnih materijala, ne može se zamisliti bez polimernih mreža. Sa druge strane, elastične polimerne mreže kao primarne strukturne jedinice postaju predmet pažnje u naučnim disciplinama u kojima ranije nisu pominjane, kao što su medicina i biohemija, a dobijaju zasluženo mesto i u nizu drugih disciplina srodnih nauci o materijalima.

Objetivo: Avaliar o papel do enfermeiro na assistência de enfermagem as crianças com transtorno do espectro do autismo. Método: O estudo será desenvolvido por meio de uma pesquisa com uma revisão integrativa da literatura nas bases de dados BVS ( Biblioteca Virtual de Saúde) e SCIELO ( Scientific Eletronic Library Online), de materiais publicados no período de 2015 e 2021, em português. Resultado: Foram analisados 24 artigos, 09 foram escolhidos para formar este artigo. Discussão: De acordo com as pesquisas na literatura os enfermeiros tem uma importância significativa na assistência e nos cuidados prestados ao portador de TEA e a sua família, tanto no atendimento quanto na orientação. Conclusão: Após a análise dos estudos foi possível concluir que o enfermeiro tem um papel muito significativo e indispensável as crianças portadora de TEA, necessitam ter conhecimento deste transtorno em sala de aula, mais especificamente na disciplina de saúde mental que foca tão pouco em transtornos o que acaba deixando os estudantes leigos após a formação.

Functional recovery after spinal cord injury is guided by the formation of new spinal detour circuits. The authors show that the formation of these circuits is enhanced by targeted chemogenetic stimulation of supraspinal and spinal neuron populations. Supraspinal and spinal coordinated stimulation potentiated behavioral recovery.

Sexual violence represents a widespread social problem associated with serious lifelong consequences. In many cases, an outcome of sexual violence is the victim's unwanted pregnancy, usually ended in an abortion. The objective of this paper is to report five rape cases, including rapes of a minor and young woman, two incest cases and a case of human trafficking for sexual exploitation, where every case resulted in the victim's pregnancy. In each case, pregnancy was terminated in the first trimester or at the beginning of the second trimester in the relevant medical center or clinic. Fresh fetal blood or aborted tissue samples were delivered to our laboratory in order to perform paternity testing for the purpose of proving the crime. DNA extraction using Qiagen Dneasy™ Tissue Kit was optimized according to the sample type. Amplification of autosomal STR (Short Tandem Repeat) markers was performed using the PowerPlex®16 System. In two cases, mixtures of maternal and fetal DNA in the aborted fetal material were found. Using the LRmix Studio v.2.1.5 Software for interpreting DNA mixtures based on a probabilistic model, the likelihoods of maternal contribution and presence of fetal allelic variants inherited from the alleged father/suspect were calculated. Based on these results, we confirmed the presence of assumed fetal fractions (determined before software analysis) in the mixtures. In all cases, positive paternity proved the crime (probabilities of paternity >99.9999%). This cases report once again pointed out the importance of DNA analysis in the process of clarifying and solving forensic cases and demonstrated that the LRmix Studio v.2.1.5 Software can deal with complex cases such as sexual assaults.

This is the first study to use moss transplants as a biomonitoring technique and inductively plasma mass spectrometry to investigate airborne potentially toxic element pollution and air quality in urban areas on a regional scale across nine Southeastern European countries. The mosses in bags were exposed for 2 months during the winter season (2019/2020) at five sites in each of the selected urban areas next to air quality (AQ) monitoring stations. The sites were selected concerning different land use classes (typical urban, residential, urban background, and rural sites). The concentrations of 35 elements were determined using inductively coupled plasma mass spectrometry. A comparison with elemental analysis performed by total reflection X-ray fluorescence is also presented. Concentrations of the potentially toxic element in moss bags exposed in urban areas were significantly higher, while those exposed in rural areas did not differ significantly from the initial content of unexposed moss. Linear regression analysis revealed no significant differences between the average concentrations of elements in H. cupressiforme and S. girgensohnii moss bags (linearity R ^2 = 0.94). It shows similar trends in elements in both moss species. The median values of contamination factors in both moss species ranged mostly from 1.2 to 2.17, indicating the exposure sites show no contamination to slight contamination status (C1 to C2 scales). High values of relative accumulation factors were found for Cr, Cu, Fe, Ni, Sb, and V in moss bags, indicating these elements are the most abundant in most sampling sites, which may increase human exposure through inhalation and could lead to harmful health problems.

Background The aim of the study was to explore the mutual relationship between oxidative stress, inflammation and metabolic biomarkers in subjects with prediabetes (PRE), newly diagnosed type 2 diabetes patients (NT2D) and overt type 2 diabetes (T2D) using principal component analysis (PCA) as a thorough statistical approach. Methods Glycated hemoglobin, lipid parameters, inflammation (IL-6, CRP and fibrinogen) and oxidative stress markers pro-oxidants (AOPP, PAB, TOS) and antioxidants (PON1, tSHG, TAS) were measured. PCA was applied to explore the factors that the most strongly influenced glucoregulation. Results A total of 278 subjects were (i.e., 37 PRE, 42 NT2D and 99 T2D) were compared with 100 healthy subjects as a control group (CG). PCA emphasized 4 different factors explaining 49% of the variance of the tested parameters: oxidative stress-dyslipidemia related factor (with positive loading of TG and tSHG, and with negative loading of HDL-c and TAS), dyslipidaemia related factor (i.e., total cholesterol and LDL-c, both with positive loading), Anthropometric related factor (i.e., waist and hip circumference, both with positive loading) and oxidative stressInflammation related factor (i.e., PAB, fibrinogen, and CRP all with positive loading). Out of these 4 factors, only oxidative stress - dyslipidaemia related factor showed a significant predictive capability towards poor glucoregulation. An increase in this factor by one unit showed a 1.6 times higher probability for poor glucoregulation. Conclusions Redox imbalance (determined with lower TAS and higher tSHG), in addition to higher TG and lower HDLc was associated with poor glucoregulation.