This paper presents the first estimation of polymorphism of the Bosnian-Herzegovinian-Croatian Shepherd dog Tornjak in Bosnia and Herzegovina using 10 microsatellite loci, which are an integral part of StockMarks® for Canine Genotyping Kit (Applied Biosystems, Foster City, CA, USA). Ten microsatellite loci used in this study are appropriate for assessing the genetic diversity for this breed. Measures of genetic diversity were estimated based on allelic and genotypic calculations, observed (HO) and expected (HE) heterozygosities, deviations from Hardy-Weinberg equilibrium and polymorphism information content (PIC). The lowest genetic diversity was estimated for locus PEZ20, and the highest for PEZ6 locus. Observed and expected mean heterozygosities were 0.7261 and 0.7392, respectively. Statistically significant deviation (p<0.05) from Hardy-Weinberg equilibrium was found for PEZ1, PEZ12, PEZ3 and PEZ6 loci. The PIC values suggested that all markers (100%) are very informative (PIC > 0.5) in terms of their suitability for genetic diversity studies. When all observed parameters are taken into account (observed and expected heterozygosities, PIC, number of detective and effective alleles, number of detected and maximum possible genotypes, major allele frequency and major allele frequency index), we can conclude that PEZ6 locus shows the highest genetic diversity while PEZ3 displays the lowest. However, assuming values of observed and expected heterozygosities, as well as PIC, we consider loci PEZ20 to be the least diverse, but this locus has more effective alleles and more genotypes present than PEZ3. These preliminary results are the first genetic diversity survey of the Bosnian-Herzegovinian-Croatian Shepherd dog Tornjak in Bosnia and Herzegovina and could be useful to the dog breeders in designing and managing breeding strategies. Summarizing the information above, we can conclude that the population of the Bosnian-Herzegovinian-Croatian Shepherd dog Tornjak from B&H is not affected by substantial loss of genetic diversity. Results of our study indicate presence of reasonably high level of genetic variability and lead to a better understanding of this dog breed.

Lameness in sheep, especially in semi-extensive breeding of Dubska pramenka, which starts in the interdigital region, was the motive of our morphological and histological research, in order to and in some way, bring the structure of the interdigital sinus in correlation with the appearance of this pathological state that causes extensive economical losses in the domestic sheep breeding,. The anatomical location of the interdigital diverticulum, its histological structure, which point to abundance of sebaceous glands around the hair follicles surrounding the interdigital opening and interdigital glands in deeper layers, point to the suggestion that their secretion increases due to poor hygienic keeping conditions. Pathogens - anaerobic microorganisms, which are most commonly causing the contagious lameness find the ideal conditions for their growth and reproduction because the secretion of these glands is likely "to close" the only "air" communication - interdigital opening.

The possibility of using different probiotic products primarily in animal food represents a special direction in the modern production of broiler meat. Intensive broiler production has been improved in the last few decades especially in the fields of genetics, nutrition and technology, in order to achieve maximum growth broilers, the optimal utilization of food and high yields, especially white meat. Considering the above mentioned research was conducted in order to examine the impact of commercial probiotic products on the body conformation of the carcasses of chickens after 42 days of fattening. In experiment was used a total of 30 chickens provenience Cobb divided into two groups (P and K). Chickens from both groups were vaccinated according to the plan and program of immunoprophylaxis, and selected technical and technological solutions of accommodation, as well as feeding and watering chickens were in accordance with the recommendations of the selectors for the floor system rearing. Chickens of experimental group (P) were given in drinking water, a commercial probiotic Probios ®, a soluble powder for poultry, product of a company Chr. Hansen A / S, Denmark. Probiotic treatment of experimental groups of chickens was conducted the first three days of life and three days during the vaccination of chickens (the day before, on the day and the day after vaccination). Measures of body conformation (the breast circumference, keel length, breast depth, drumsticks circumference, shank length and breast angle) were determined on carcasses after the fattening of 42nd day using standard tools in livestock breeding. The results obtained from this study suggest that the usage of commercial probiotics in broiler chickens in intensive fattening has proved to be statistically significant on tested measure of body conformation.