The study included a total of 127 sheep milk samples from two different areas (Livno and Travnik) in summer feeding period (July, August and September). Fatty acids in milk were determined by gas chromatography (GC). The animals were marked with the appropriate number of ear tags on the basis of which we always took samples from the same animals through different periods. Fatty acids in milk were determined by gas chromatography and the following fatty acids composition: butyric acid, caproic acid, caprylic acid, capric acid, stearic acid, oleic acid, linoleic acid, arachidonic acid, eicosapentaenoic acid, docosahexaenoic acid, rumenic acid. The fatty acid content of sheep's milk in this study showed a tendency of variation, both within and between sampling areas, and characterized by its relatively high content of saturated fatty acid (SFA) during the period of harvest.
A continual development of science, engineering and technology resulted in usage of reliable, durable instruments and devices which maintenance costs are reduced to minimum during their life-time. Nowadays, usage of flow-meter based on the Coriolis force on measuring places is increasing. An everyday flow measurement is known problem of calculation between mass and volumetric flow and it is stressed in fiscal measurement especially in the oil industry. To get accurate mass units from volumetric, beside volumetric meter, it is necessary to include additional measurement apparatus for measurement of temperature, pressure, density and other variables. All mentioned parameters in continual flow process makes calculation harder and in many cases makes uncertainty higher. In this paper, important parameters for proper design and selection of a certain flow meters will be presented. Also, basics of measurement uncertainty will be presented under conditions of relatively low measurement passes as well as basic information about appearing of Coriolis force and Coriolis mass flow meter.
Microscopic analysis of the skin and cuticle of wool fibers in Dubska pramenka show different qualitative parameters. The research study included different parts of the body and the samples of the wool fibers from different regions of the body. Histological description shows difference in the basic structurers of the skin in Dubska pramenka. A very pronounced thin epidermis, while dermis and hypodermis are more developed on the samples from the shoulder. Cornified flakes-cuticle of the root of the tail was of a fine structure, and cornified flakes from the rump showed the features of the rough wool fibers. “Transitional form of cuticle” was dominant on the shoulder. The qualitative parameters have a significant influence on the overall quality of wool in general, and the research contributes to a greater usability value of the wool and development of livestock farming in areas in a broader sense.
This paper presents the first estimation of polymorphism of the Bosnian-Herzegovinian-Croatian Shepherd dog Tornjak in Bosnia and Herzegovina using 10 microsatellite loci, which are an integral part of StockMarks® for Canine Genotyping Kit (Applied Biosystems, Foster City, CA, USA). Ten microsatellite loci used in this study are appropriate for assessing the genetic diversity for this breed. Measures of genetic diversity were estimated based on allelic and genotypic calculations, observed (HO) and expected (HE) heterozygosities, deviations from Hardy-Weinberg equilibrium and polymorphism information content (PIC). The lowest genetic diversity was estimated for locus PEZ20, and the highest for PEZ6 locus. Observed and expected mean heterozygosities were 0.7261 and 0.7392, respectively. Statistically significant deviation (p<0.05) from Hardy-Weinberg equilibrium was found for PEZ1, PEZ12, PEZ3 and PEZ6 loci. The PIC values suggested that all markers (100%) are very informative (PIC > 0.5) in terms of their suitability for genetic diversity studies. When all observed parameters are taken into account (observed and expected heterozygosities, PIC, number of detective and effective alleles, number of detected and maximum possible genotypes, major allele frequency and major allele frequency index), we can conclude that PEZ6 locus shows the highest genetic diversity while PEZ3 displays the lowest. However, assuming values of observed and expected heterozygosities, as well as PIC, we consider loci PEZ20 to be the least diverse, but this locus has more effective alleles and more genotypes present than PEZ3. These preliminary results are the first genetic diversity survey of the Bosnian-Herzegovinian-Croatian Shepherd dog Tornjak in Bosnia and Herzegovina and could be useful to the dog breeders in designing and managing breeding strategies. Summarizing the information above, we can conclude that the population of the Bosnian-Herzegovinian-Croatian Shepherd dog Tornjak from B&H is not affected by substantial loss of genetic diversity. Results of our study indicate presence of reasonably high level of genetic variability and lead to a better understanding of this dog breed.
The international standard ISO/IEC 17020 contains the requirements for work of various inspection bodies which aim to raise the level of their activities, as well as the confidence level of clients in performing inspection activities. Furthermore, the standard is aligned with other standards of series standards ISO/IEC 17000 and contains certain parts of guideline IAF/ILAC A4 as well as the mandatory parts of ISO/PAS documents. The standard is used as the reference document during an assessment process of inspection bodies by accreditation bodies. Based on the assessment, inspection bodies prove the fulfillment of the standard requirements, respectively their competencies for performing inspection activities and gain market confidence. The paper describes all necessary steps which should be taken into account by an inspection body so that it can fulfill general and technical requirements of the standard and demonstrate the necessary competencies for performing inspection activities. Especially, the paper will pay attention to impartiality, independence and competence of personnel, traceability of equipment, inspection methods, quality management system of an inspection body, etc.
The aim of this study was to determine the value of some hematological parameters in the blood plasma of Holstein-Friesian calves, and their effect on the nature of the changes in the first month of age. For this experiment we selected 20 calves (10 male and 10 female) aged one month, farm-raised, whose diet was based on the milk and starter mixture for the calves. Blood plasma of the examined animals was tested for the following hematological parameters: red blood cell count, white blood cell count, hematocrit, hemoglobin and red blood cell indices MCH, MCHC, MCV. Statistical analysis revealed decreased values of all hematological parameters in tested animals except RBC, PCV and MCV. The results indicate a highly significant impact of these values in male calves. The coefficient of correlation between the tested hematological parameters indicates the level of justification ranging from significant to highly significant differences in tested animals. Key words: calves, erythrocytes, hemoglobin, hematocrit, hematological indices
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