Fluoride release is important characteristic of glass-ionomer cements. Quantity of fluoride ions released from the glass-ionomer cements has major importance in definition of their biological activity. The objectives of this study were to define the quantity of fluoride ions released from the experimental glass-ionomer cements and to define the effect of fluoride ions released from the experimental glass-ionomer cements on their cytotoxicity. Concentrations of the fluoride ions released in the evaluated glass-ionomer cements were measured indirectly, by the fluoride-selective WTW, F500 electrode potential, combined with reference R503/D electrode. Statistical analyses of F-ion concentrations released by all glass-ionomers evaluated at two time points, after 8 and after 24 hours, show statistically higher fluoride releases from RMGICs: Vitrebond, Fuji II LC and Fuji Plus, when compared to conventional glass-ionomer cements: Fuji Triage, Fuji IX GP Fast and Ketac Silver, both after 8 and after 24 hours. Correlation coefficient between concentrations of fluoride ion released by evaluated glass-ionomer cements and cytotoxic response of UMR-106 osteoblast cell-line are relatively high, but do not reach levels of biological significance. Correlation between concentrations of fluoride ion released and cytotoxic response of NIH3T3 mouse fibroblast cell line after 8 hours is high, positive and statistically significant for conventional GICs, Fuji Triage and Fuji IX GP Fast, and RMGIC, Fuji II LC. Statistically significant Correlation coefficient between concentrations of fluoride ion released and cytotoxic response of NIH3T3 cell line after 24 hours is defined for RMGIC Fuji II LC only.

Fluoride release is important characteristic of glass-ionomer cements. Quantity of fl uoride ions released from the glass-ionomer cements has major importance in defi nition of their biological activity. Th e objectives of this study were to defi ne the quantity of fl uoride ions released from the experimental glass-ionomer cements and to defi ne the eff ect of fl uoride ions released from the experimental glass-ionomer cements on their cytotoxicity. Concentrations of the fl uoride ions released in the evaluated glass-ionomer cements were measured indirectly, by the fl uoride-selective WTW, F electrode potential, combined with reference R/D electrode. Statistical analyses of F-ion concentrations released by all glass-ionomers evaluated at two time points, after  and after  hours, show statistically higher fl uoride releases from RMGICs: Vitrebond, Fuji II LC and Fuji Plus, when compared to conventional glass-ionomer cements: Fuji Triage, Fuji IX GP Fast and Ketac Silver, both after  and after  hours. Correlation coeffi cient between concentrations of fl uoride ion released by evaluated glass-ionomer cements and cytotoxic response of UMR- osteoblast cell-line are relatively high, but do not reach levels of biological signifi cance. Correlation between concentrations of fl uoride ion released and cytotoxic response of NIHT mouse fi broblast cell line after  hours is high, positive and statistically signifi cant for conventional GICs, Fuji Triage and Fuji IX GP Fast, and RMGIC, Fuji II LC. Statistically signifi cant Correlation coeffi cient between concentrations of fl uoride ion released and cytotoxic response of NIHT cell line after  hours is defi ned for RMGIC Fuji II LC only. ©  Association of Basic Medical Sciences of FB&H. All rights reserved

Objective: To examine process inside plaque after application of three topical fluoride solutions: 1% TiF4, 1% NaF and Aminfluorid soluti on. Efficiency of t...

Simultaneous separation of eleven steroid hormones and synthetic anabolics: progesterone, trenbolone acetate melengestrol acetate, 17-β-estradiol, 19-nortestosterone, fluoxymesterone. norethandrolone, 4-chloro-δ-1-methyl testosterone, clostebol acetate, 6-β-hydroxymethandienone and oxymetholone, was performed on HPTLC plates, 10 x 10 cm, silicagel 60 F 254 (Merck) by. horizontal elution in chloroform-acetone mobile phase. The investigated steroids were successfully visualised under UV light (254 nm). and after spraying with an ethanolic solution of/Moluenesulphonic acid. The efficacy of chromatographic system was checked using simulated real samples for some of the examined steroids, melengestrol acetate and trenbolone acetate, usually misused as growth promoters in cattle and stored unchanged in animal tissue.

Derivatives of chlorophenoxy carboxylic acids have been the first class of herbicides in continuous use since 1947. The main interest for these substances is due to their evident chronic toxicity and carcinogenic effect. On the other hand, they can cause acute toxicity and have significant role in suicidal attempts. In this paper we have investigated analytical approaches that could be used for rapid identification and determination of chlorophenoxy herbicides in modestly equipped laboratories for the clinical toxicology. Thin layer chromatography on cellulose layer using neutral red as ion pairing reagent gave the best results in separation of different herbicides, making possible visualisation at the daylight without further reagents or equipment. High performance liquid chromatography (HPLC) methods for separation on C8 and C18 phases with and without ion-pairing reagent were compared. It was found that HPLC on C18 phase utilising ion-suppression mode has the best reproducibility, linearity and mass limit of detection suitable for quantification of chlorophenoxy herbicides after acute poisoning.

In this paper we present introduction and development of some new analytical methods for identification of anabolic steroids, their metabolites and certain hormones, especially determination of exogenous testosterone by means of gas chromatography-mass spectrometry. Identification of central nervous stimulants and corticosteroides has been performed by high performance liquid chromatography. In desire to achieve better results, to increase strength and endurance, to sharpen reflexes and to reduce stress and anxiety athletes as well as other people use different pharmacological substances, hormones or even illicit drugs. Use of these substances without medical supervision can lead to adverse effects to one's health or even cause a death. At the same time, use of such substances means a kind of cheat that could not be accepted. This is why International Olympic Committee started at 1968 with official doping control that is permanently carried out and continuously increasing number of banned substances. Doping control demands for discover and development of new sensitive and specific methods for detection of banned substances and their metabolites in urine and blood.

Metabolite of 17-methyl-17-hydroxy-2-(hydroxymethylen)-androstan-3-one (oxymetholane) in urine after a single oral administration was monitored by gas chromatography+mass spectrometry. During the investigation prepared TMS-ethers and TMS-enol-ethers of conjugated steroid fraction two new metabolites of oxymetholone have been identified: tetrahydrooxymetholone and tetrahydro-6-hydroxy-oxymetholone.