Fluoride release is important characteristic of glass-ionomer cements. Quantity of fluoride ions released from the glass-ionomer cements has major importance in definition of their biological activity. The objectives of this study were to define the quantity of fluoride ions released from the experimental glass-ionomer cements and to define the effect of fluoride ions released from the experimental glass-ionomer cements on their cytotoxicity. Concentrations of the fluoride ions released in the evaluated glass-ionomer cements were measured indirectly, by the fluoride-selective WTW, F500 electrode potential, combined with reference R503/D electrode. Statistical analyses of F-ion concentrations released by all glass-ionomers evaluated at two time points, after 8 and after 24 hours, show statistically higher fluoride releases from RMGICs: Vitrebond, Fuji II LC and Fuji Plus, when compared to conventional glass-ionomer cements: Fuji Triage, Fuji IX GP Fast and Ketac Silver, both after 8 and after 24 hours. Correlation coefficient between concentrations of fluoride ion released by evaluated glass-ionomer cements and cytotoxic response of UMR-106 osteoblast cell-line are relatively high, but do not reach levels of biological significance. Correlation between concentrations of fluoride ion released and cytotoxic response of NIH3T3 mouse fibroblast cell line after 8 hours is high, positive and statistically significant for conventional GICs, Fuji Triage and Fuji IX GP Fast, and RMGIC, Fuji II LC. Statistically significant Correlation coefficient between concentrations of fluoride ion released and cytotoxic response of NIH3T3 cell line after 24 hours is defined for RMGIC Fuji II LC only.

In this paper we present introduction and development of some new analytical methods for identification of anabolic steroids, their metabolites and certain hormones, especially determination of exogenous testosterone by means of gas chromatography-mass spectrometry. Identification of central nervous stimulants and corticosteroides has been performed by high performance liquid chromatography. In desire to achieve better results, to increase strength and endurance, to sharpen reflexes and to reduce stress and anxiety athletes as well as other people use different pharmacological substances, hormones or even illicit drugs. Use of these substances without medical supervision can lead to adverse effects to one's health or even cause a death. At the same time, use of such substances means a kind of cheat that could not be accepted. This is why International Olympic Committee started at 1968 with official doping control that is permanently carried out and continuously increasing number of banned substances. Doping control demands for discover and development of new sensitive and specific methods for detection of banned substances and their metabolites in urine and blood.

The location of metallurgic complex, together with power plants and coke production in an urban centre, results in an appropriate atmospheric pollution part of which are organic compounds in a significant amount. A long-term exposit ion to some organic compounds may result in chronic toxic or genotoxic effects, so identification of such compounds means a necessity in planning of permanent monitoring. It was the aim of this paper. Concerning Lite nature of air pollution in this case, various classes of organic compounds were expected in atmosphere which caused a different sampling procedure to be applied. Samples were collected on glass fibre filters, activated charcoal, silica-gel Layers, on filters made of specially prepared coal tissue and in impinges filled with toluene. The identification was performed by gas chromatography and gas chromatography — mass spectrometry. Alkyl substituted phenols, alkyl derivatives of benzene, polycyclic aromatic hydrocarbons and their alkyl derivatives, normal, branched and cyclic alkanes and alkenes, aminonaphthalenes, aliphatic alcohols and ketones and heterocyclic compounds with nitrogen and sulphur were identified.

Alprenolole is subject to a fast-metabolic transformation like other beta blocking agents. Until now 4-hidroxy alprenolol and N-dcsizopropyl alprenolol are identified as products of such metabolic transformations. In the paper Identification of methoxy-hydroxy alprenolol, a new metabolite of alprenolol, performed by selective derivatization and GC-MS structure examination. is described.