—The problem of transport optimization is of great importance for the successful operation of distribution companies. To successfully find routes, it is necessary to provide accurate input data on orders, customer location, vehicle fleet, depots, and delivery restrictions. Most of the input data can be provided through the order creation process or the use of various online services. One of the most important inputs is an estimate of the unloading time of the goods for each customer. The number of customers that the vehicle serves during the day directly depends on the time of unloading. This estimate depends on the number of items, weight and volume of orders, but also on the specifics of customers, such as the proximity of parking or crowds at the unloading location. Customers repeat over time, and unloading time can be calculated from GPS data history. The paper describes the innovative application of machine learning techniques and delivery history obtained through a GPS vehicle tracking system for a more accurate estimate of unloading time. The application of techniques gave quality results and significantly improved the accuracy of unloading time data by 83.27% compared to previously used methods. The proposed method has been implemented for some of the largest distribution companies in Bosnia and Herzegovina.

Background & Aims YAP (Yap1) and TAZ (Wwtr1) are transcriptional co-activators and downstream effectors of the Hippo pathway, which play crucial roles in organ size control and cancer pathogenesis. Genetic deletion of YAP/TAZ has shown their critical importance for embryonic development of the heart, vasculature, and gastrointestinal mesenchyme. The aim of this study was to determine the functional role of YAP/TAZ in adult smooth muscle cells in vivo. Methods Because YAP and TAZ are mutually redundant, we used YAP/TAZ double-floxed mice crossed with mice that express tamoxifen-inducible CreERT2 recombinase driven by the smooth muscle–specific myosin heavy chain promoter. Results Double-knockout of YAP/TAZ in adult smooth muscle causes lethality within 2 weeks, mainly owing to colonic pseudo-obstruction, characterized by severe distension and fecal impaction. RNA sequencing in colon and urinary bladder showed that smooth muscle markers and muscarinic receptors were down-regulated in the YAP/TAZ knockout. The same transcripts also correlated with YAP/TAZ in the human colon. Myograph experiments showed reduced contractility to depolarization by potassium chloride and a nearly abolished muscarinic contraction and spontaneous activity in colon rings of YAP/TAZ knockout. Conclusions YAP and TAZ in smooth muscle are guardians of colonic contractility and control expression of contractile proteins and muscarinic receptors. The knockout model has features of human chronic intestinal pseudo-obstruction and may be useful for studying this disease.

The main OFDM drawbacks are Carrier Frequency Offset (CFO) and large Peak-to-Average Power Ratio (PAPR), which both degrade the Bit Error Rate (BER). Specifically, we consider here clipping or any other PAPR reduction method sufficient to prevent the nonlinear high-power amplifier from generating errors. Moreover, in small cells, the signal-to-noise ratio is large, while the small time dispersion allows the OFDM symbol cyclic prefix to prevent intersymbol interference. This retains the CFO to solely determine the BER and vice versa, enabling indirect estimation of CFO-induced phase distortion by simple BER testing. However, a particular problem is measuring very low BER values (generated by alike residual CFO), which could last a long time in order to acquire statistically enough errors. The test time can be drastically reduced if the noise margin is reduced in a controllable way, by adding the interfering signal to each subcarrier at the receiver. This approach is shown to enable efficient and accurate short-term BER (and so CFO phase error) testing.

To develop a consensus on diagnosis and treatment of acromioclavicular joint instability. A consensus process following the modified Delphi technique was conducted. Panel members were selected among the European Shoulder Associates of ESSKA. Five rounds were performed between October 2018 and November 2019. The first round consisted of gathering questions which were then divided into blocks referring to imaging, classifications, surgical approach for acute and chronic cases, conservative treatment. Subsequent rounds consisted of condensation by means of an online questionnaire. Consensus was achieved when ≥ 66.7% of the participants agreed on one answer. Descriptive statistic was used to summarize the data. A consensus was reached on the following topics. Imaging: a true anteroposterior or a bilateral Zanca view are sufficient for diagnosis. 93% of the panel agreed on clinical override testing during body cross test to identify horizontal instability. The Rockwood classification, as modified by the ISAKOS statement, was deemed valid. The separation line between acute and chronic cases was set at 3 weeks. The panel agreed on arthroscopically assisted anatomic reconstruction using a suspensory device (86.2%), with no need of a biological augmentation (82.8%) in acute injuries, whereas biological reconstruction of coracoclavicular and acromioclavicular ligaments with tendon graft was suggested in chronic cases. Conservative approach and postoperative care were found similar A consensus was found on the main topics of controversy in the management of acromioclavicular joint dislocation. Each step of the diagnostic treatment algorithm was fully investigated and clarified. Level V.

The scope, scale, and intensity of real, as well as potential attacks, on the Smart Grid have been increasing and thus gaining more attention. An important component of Smart Grid cybersecurity efforts addresses the availability and access to the power and related information and communications infrastructures. We overload the term, Denial-of-Service (DoS), to refer to these attacks in the Smart Grid. In this paper, we provide a holistic and methodical presentation of the DoS attack taxonomies as well as a survey of potential solution techniques to help draw a more concerted and coordinated research into this area, lack of which may have profound consequences. To the best of our knowledge, the literature does not have such a comprehensive survey study of the DoS attacks and solutions for the Smart Grid.

Recent studies show that several tree species are spreading to higher latitudes and elevations due to climate change. European beech, presently dominating from the colline to the subalpine vegetation belt, is already present in upper montane subalpine forests and has a high potential to further advance to higher elevations in European mountain forests, where the temperature is predicted to further increase in the near future. Although essential for adaptive silviculture, it remains unknown whether the upward shift of beech could be assisted when it is mixed with Norway spruce or silver fir compared with mono-specific stands, as the species interactions under such conditions are hardly known. In this study, we posed the general hypotheses that the growth depending on age of European beech in mountain forests was similar in mono-specific and mixed-species stands and remained stable over time and space in the last two centuries. The scrutiny of these hypotheses was based on increment coring of 1240 dominant beech trees in 45 plots in mono-specific stands of beech and in 46 mixed mountain forests. We found that (i) on average, mean tree diameter increased linearly with age. The age trend was linear in both forest types, but the slope of the age–growth relationship was higher in mono-specific than in mixed mountain forests. (ii) Beech growth in mono-specific stands was stronger reduced with increasing elevation than that in mixed-species stands. (iii) Beech growth in mono-specific stands was on average higher than beech growth in mixed stands. However, at elevations > 1200 m, growth of beech in mixed stands was higher than that in mono-specific stands. Differences in the growth patterns among elevation zones are less pronounced now than in the past, in both mono-specific and mixed stands. As the higher and longer persisting growth rates extend the flexibility of suitable ages or size for tree harvest and removal, the longer-lasting growth may be of special relevance for multi-aged silviculture concepts. On top of their function for structure and habitat improvement, the remaining old trees may grow more in mass and value than assumed so far.

Recent studies show that several tree species are spreading to higher latitudes and elevations due to climate change. European beech, presently dominating from the colline to the subalpine vegetation belt, is already present in upper montane subalpine forests and has a high potential to further advance to higher elevations in European mountain forests, where the temperature is predicted to further increase in the near future. Although essential for adaptive silviculture, it remains unknown whether the upward shift of beech could be assisted when it is mixed with Norway spruce or silver fir compared with mono-specific stands, as the species interactions under such conditions are hardly known. In this study, we posed the general hypotheses that the growth depending on age of European beech in mountain forests was similar in mono-specific and mixed-species stands and remained stable over time and space in the last two centuries. The scrutiny of these hypotheses was based on increment coring of 1240 dominant beech trees in 45 plots in mono-specific stands of beech and in 46 mixed mountain forests. We found that (i) on average, mean tree diameter increased linearly with age. The age trend was linear in both forest types, but the slope of the age–growth relationship was higher in mono-specific than in mixed mountain forests. (ii) Beech growth in mono-specific stands was stronger reduced with increasing elevation than that in mixed-species stands. (iii) Beech growth in mono-specific stands was on average higher than beech growth in mixed stands. However, at elevations > 1200 m, growth of beech in mixed stands was higher than that in mono-specific stands. Differences in the growth patterns among elevation zones are less pronounced now than in the past, in both mono-specific and mixed stands. As the higher and longer persisting growth rates extend the flexibility of suitable ages or size for tree harvest and removal, the longer-lasting growth may be of special relevance for multi-aged silviculture concepts. On top of their function for structure and habitat improvement, the remaining old trees may grow more in mass and value than assumed so far.

Purpose The present study aims to explore “what” type of knowledge is shared, “how” it is shared and what information and communication tools are used to share knowledge among health-care professionals in the context of emerging “digital laggard” country in the South-Eastern European region (i.e. Bosnia and Herzegovina [B&H]). Design/methodology/approach This study applies the qualitative research methodology and thematic analysis for collecting, analysing and interpreting data. Primary data is collected using semi-structured interviews (n = 9). Key informants are employees of the main primary health-care institution in the capital of B&H. Findings The results indicate that knowledge sharing among primary health-care practitioners is the interplay of formal and informal exchange of knowledge, facilitated by both traditional communication media (face-to-face interaction) and ICT-based tools. In regards to ICT tools, our findings suggest that instant messing applications (predominantly Viber) are viewed as common media for sharing tacit knowledge within a community of practice. Research limitations/implications This study is limited to one emerging digital laggard country; thus, future research regarding ICT-based knowledge sharing in other emerging digital laggard countries is welcomed. Originality/value In the context of “digital laggard”, societies, informal, spontaneous and dynamic system of social interactions can be a successful substitute for ICT-based formalised and structured system of knowledge sharing.

Luteolin and delphinidin are the flavonoids with known protective roles. They inhibit genotoxic effects induced by halogenated boroxine (HB) in vitro. Statistically significant decrease in the frequency of micronuclei and nuclear buds and suppression of the occurrence of aberrant cells were observed before, but mechanism of its anti-genotoxic activity is still not clear. In our experiment we aimed to quantify HB effects on the relative expression of CAT (catalase) gene and explore antioxidative effects of luteolin and delphinidin via restoration of CAT gene activity. Cell cultures from peripheral blood lymphocytes of five healthy donors were established and treated with independent and concomitant treatments of HB with luteolin or delphinidin. Total RNA was isolated from harvested cells and reverse-transcribed. SYBR based Real-Time PCR amplification method was used. Analysis of results included normalization of ratio of target (CAT) and housekeeping (GAPDH) gene and statistical analysis (REST®). Luteolin itself lead to downregulation of relative CAT gene expression as well as HB. But simultaneous treatment of HB and bioflavonoids lead to upregulation. Delphinidin as independent treatment and as simultaneous treatment caused upregulation of relative CAT gene expression. Obtained results may indicate protective role of delphinidin and luteolin to oxidative damage caused by HB, and also that new approaches to the treatment applications of HB should include bioflavonoids and monitoring corresponding antioxidant system. Our findings indicate that there is a quantifiable effect of luteolin and delphinidine on antioxidant genes which could be used in exact monitoring of oxidative stress related events.

Streptococcosis causes economic losses due to the high mortality in Nile tilapia (Oreochromis niloticus). The present study was carried out to detect the streptococci by PCR and chemical analysis in cultured tilapia fish. A total of 100 cultured tilapia fishes and 20 water samples were collected from 4 different fish farms at different locations in Kafr El-sheikh Governorate for bacteriological and chemical analysis. The results of water quality parameters examination revealed that the mean values of pH, dissolved oxygen, unionized ammonia, and nitrite were 8.2 ± 0.73, 7.44 ± 0.54 parts per million (ppm), 0.05 ± 0.008 ppm, and 0.00 ppm, respectively. All water quality parameters except for ammonia were within the permissible limit. The bacterial isolation results revealed 38 % positive samples for Streptococcus species isolated from tilapia fishes and 40 % were positive for Streptococcus species isolated from water samples. Of those 38 positive fish samples, 25 (65.78%) were Enterococcus faecalis, 15 (39.47%) were Streptococcus pyogenes, 6 (15.78%) were Enterococcus faecium, 5 (13.15 %) were Streptococcus agalactiae, and 3 (7.89 %) were Streptococcus iniae. On the other hand, from 8 positive farms water samples, 7 (87.5%) were Enterococcus faecalis, 2 (25%) were Streptococcus pyogenes , 2 (25%) were Enterococcus faecium, 3 (37.5%) were Streptococcus agalactiae, and 1 (12.5%) was Streptococcus iniae . Using mPCR to identify Streptococcus for some fish and water isolates, 6 Enterococcus faecalis with 310 base per (bp) were detected while 3 Streptococcus agalactiae with 153 bp and 2 Enterococcus faecium at 215 bp were detected. However, the mPCR from Streptococcus species directly from organs from fish and water samples revealed that 5 Enterococcus faecalis were detected at 310 bp while 2 Streptococcus agalactiae was identified at 153 bp. The biochemical results indicated that the infected fish with the streptococcal species had reduced total protein, albumin, and globulin in the blood serum while total cholesterol, urea, creatinine levels, and AST, ALT, GPX, CAT, SOD activities significantly increased, compared to noninfected fish. O R IG IN A L A R T IC L E p i: S 2 3 2 2 4 5 6 8 2 0 0 0 0 3 7 -1 0 R eeiv ed : 1 1 Ju l 2 0 2 0 A ccep ed : 2 0 A u g 2 0 2 0

The research included 116 subjects who have declared that they had participated in sports, sport activities and recreation. It is a targeted sample, competent to declare their views on questions of why they practice sports and sport activities and what are the reasons (causes) for young people to quit the sport which they participated in. The results obtained on reasons for participation in sports and sport activities show that good physical shape is the primary reason, followed by health, social interaction, and friendship and making acquaintances, while a sporting career and financial benefit are by far lowest on the scale. When it comes to reasons why young people quit sports and sport activities, the majority quit because of indolence, followed by injuries, social circles, and lack of free time, pressure from overambitious parents, poor attitude of the coach, excessive exertion, and ultimately, lack of talent. When it comes to differences between attitudes on quitting sports between the sexes, occupations, smokers/non-smokers, alcohol consumers/non-consumers, and types of residential areas, the results have shown that neither the subject’s sex nor alcohol consumption have an impact on attitudes on reasons for quitting sports. Some difference was observed in level of education (high school or university student), smoking (smokers or non-smokers), and type of residential area (city, suburbs, countryside).

Distributed and collaborative computer-aided design (CAD) environments include building information modeling (BIM) and geographical information systems (GISs) in civil engineering and architecture, or product data management/ product life cycle management (PDM/PLM) in mechanical engineering. It is essential to keep the data integrity in these computer applications as it contributes to building users’ confidence in CAD/BIM/PDM data. Blockchain technology, the core founda-tion of cryptocurrencies, is increasingly being used for other purposes and could solve the data integrity issue in collaborative CAD environments. However, it has some disadvantages such as the transparency of data and the slowness of storing data in the blockchain due to distributed consensus. Increasing demand by the Industry 4.0, IoT, Smart Cities, and other initiatives could foster the best what blockchain has to offer: data integrity, reliability, and traceability. This chapter explains how blockchain works, how can it be utilized in distributed CAD environments, what are the major challenges for implementation, and how CAD vendors could use it to increase CAD data integrity.