Background : As antibiotic resistance  in microorganisms is  increasing and becoming a global problem, essential oils should be considered as new chemical substances with potenial therapeutic effects. Aim of the study : Determine the susceptibility of beta-lactamase clinical isolates of Pseudomonas aeruginosa to antibiotics and essential oils, define the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) for tested oils. Material and Methods : This study included 120 P. aeruginosa isolates from clinical material. A disc diffusion method was used to for determination of antibiotic and essential oil susceptibility profile. For the phenotypic detection of beta-lactamase isolates, a disk diffusion method was used according to the CLSI guidelines with clavulanic acid. MIC was determined by microdilution test in broth. MBC was recorded after determination of MIC, it was corresponded to the lowest concentration of the essential oil yielding negative subcultures after incubation at appropriate temperature for 24 h. Results : Of the three tested oils, Origanum compactum had the strongest antimicrobial effect (MIC 6.4 mg/ml - 9.3 mg / ml) on P. aeruginosa isolates followed by Thymus serpylum (MIC 13 mg / ml to 78 mg / ml) and Origanum majorana ( MIC 21,5 mg/ml do 43 mg/ml ). Conclusion : Beta-lactamase producing P. aeruginosa isolates resistant to third and forth genetration of cephalosporin antibiotics, showed susceptibility against tested essential oils.

The aim of this paper is to present the preliminary results of the monitoring study of the frequency of congenital heart disease in newborns in Tuzla Canton (Bosnia and Herzegovina), and their distribution by sex of the newborn and maternal age. The study used the data from the book of protocols and case records of the Clinic for Gynecology and Obstetrics, the University Clinical Center in Tuzla. The analysis of 8,521 newborns between 1 January 2007 and 31 December 2008 has resulted in the frequency of 1.76%, i.e. 1.31% for the mature newborns and 0.45% for the premature newborns respectively. Of the total number of registered anomalies, 10% was associated with congenital anomalies of other systems. No statistically significant differences were found in the subsamples of both mature and premature newborns when it comes to the distribution of congenital heart disease by sex of newborns and maternal age. The frequency registered in the analyzed period suggests the necessity of screening and monitoring congenital heart disease in the observed population.

Genetic structure of two human populations, indigenous and migratory population in the area of the Tuzla region, was analyzed with respect to some static and dynamic-morphological properties. The analyzed genetic data were collected by direct observation and survey of 472 pupils of school age. The investigated parameters of genetic heterogeneity were estimated by: analysis of the frequency of recessive phenotypes for every observed property, t-test analysis, and assessment of intragroup variation by analysis of Wahlund variance. Based on the obtained results it was observed that the frequency of the recessive homozygote for most of the analyzed properties was slightly higher in migratory populations. All values obtained by Wahlund variance analysis indicated a small genetic differentiation.