This paper presents major gaps and challenges for implementing the UN Decade of Ocean Science for Sustainable Development (2021-2030) in the Mediterranean region. The authors make recommendations on the scientific knowledge needs and co-design actions identified during two consultations, part of the Decade preparatory-phase, framing them in the Mediterranean Sea’s unique environmental and socio-economic perspectives. According to the ‘Mediterranean State of the Environment and Development Report 2020’ by the United Nations Environment Programme Mediterranean Action Plan and despite notable progress, the Mediterranean region is not on track to achieve and fully implement the Sustainable Development Goals of Agenda 2030. Key factors are the cumulative effect of multiple human-induced pressures that threaten the ecosystem resources and services in the global change scenario. The basin, identified as a climate change vulnerability hotspot, is exposed to pollution and rising impacts of climate change. This affects mainly the coastal zones, at increasing risk of extreme events and their negative effects of unsustainable management of key economic assets. Transitioning to a sustainable blue economy is the key for the marine environment’s health and the nourishment of future generations. This challenging context, offering the opportunity of enhancing the knowledge to define science-based measures as well as narrowing the gaps between the Northen and Southern shores, calls for a joint (re)action. The paper reviews the state of the art of Mediterranean Sea science knowledge, sets of trends, capacity development needs, specific challenges, and recommendations for each Decade’s societal outcome. In the conclusions, the proposal for a Mediterranean regional programme in the framework of the Ocean Decade is addressed. The core objective relies on integrating and improving the existing ocean-knowledge, Ocean Literacy, and ocean observing capacities building on international cooperation to reach the “Mediterranean Sea that we want”.

Abstract Background The neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), and lymphocyte-to-monocyte ratio (LMR) were analyzed in various carcinomas and their potential prognostic significance was determined. The objective of present study was to determine the correlation between these parameters and the survival of patients with small cell lung cancer (SCLC), since very few studies have been published on this type of carcinoma. Patients and methods One hundred and forty patients diagnosed with SCLC at University Hospital Center Zagreb, between 2012 and 2016 were retrospectively analyzed. Extensive-stage disease (ED) was verified in 80 patients and limited-stage disease (LD) in 60 patients. We analyzed the potential prognostic significance of various laboratory parameters, including NLR, PLR, and LMR, measured before the start of treatment. Results Disease extension, response to therapy, chest irradiation and prophylactic cranial irradiation (PCI), as well as hemoglobin, monocyte count, C-reactive protein (CRP), and lactate dehydrogenase (LDH) showed a prognostic significance in all patients. When we analyzed the patients separately, depending on the disease extension, we found that only skin metastases as well as LDH and NLR values, regardless of the cut-off value, had a prognostic significance in ED. Meanwhile, the ECOG performance status, chest irradiation, PCI, and hemoglobin and creatinine values had a prognostic significance in LD. Conclusions NLR calculated before the start of the treatment had a prognostic significance for ED, while PLR and LMR had no prognostic significance in any of the analyzed groups of patients.

Quercetin (QU), a hyperthermic sensitizer, when combined with cisplatin (CP) affects tumor growth. To determine the effects of QU and CP and their interactions, multimodal treatment in vitro and in vivo models under physiological and hyperthermic conditions was performed. In vitro, different sensitivity of T24 and UMUC human bladder cancer cells was observed after short-term exposure to QU (2 h) and CP (1 h). Effects of both compounds were investigated at low and high micromolar concentrations (1 and 50 µM, respectively) under both thermal conditions. QU acted in additive or synergistic manner in combination with CP between physiological condition and hyperthermia. As determined by 3-(4,5-dimethylthiazol2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, short-term application of QU and CP reduced cell viability. Clonal assay also indicated that combined treatment with QU and CP is lethal to bladder cancer cells in both conditions. In vivo, CP (5 or 10 mg kg−1) and QU (50 mg kg−1) acted synergistically with hyperthermia (43 °C) and inhibited tumor growth, activated immune effectors and increased mice survival. Our results demonstrate that combined treatment with CP and QU may increase death of tumor cells in physiological and hyperthermic conditions which could be clinically relevant in locoregional chemotherapy.

This article presents the third part of data on selected new and noteworthy taxa ensuing from the revision of Herbarium collection of the Institute for Nature Conservation of the Vojvodina province (PZZP). Selected taxa include 11 species, three subspecies, three varieties, one infraspecific taxon with indetermined status [stat. indet.] and one nothospecies placed within seven genera (Filago L., Ludwigia L., Luzula DC., Lycopus L., Lythrum L., Phlomis L. and Silene L.). Two varieties (Luzula campestris subsp. campestris var. elata, Silene flos-cuculi subsp. floscuculi var. latifolia) and one infraspecific taxon with indetermined status (Luzula multiflora subsp. multiflora [stat. indet.] b. uliginosa) are new for a flora of Serbia. Additionally, one species (Ludwigia palustris) and one nothospecies (Lythrum ×scabrum) are confirmed for Serbia.

Background and Purpose: Bark thickness and its share in the volume of roundwood are the most important characteristics of the bark, particularly in the process of timber harvesting, and during scaling of processed logs. Therefore it is very important to have at disposal relatively accurate data regarding these characteristics of bark for particular tree species. The main goal of this paper is to investigate the thickness of the bark and its share in the volume of roundwood of Norway spruce. Materials and Methods: The research was carried out in the area of the Canton 10 of the Federation of Bosnia and Herzegovina and it encompassed 393 trees of Norway spruce from 10 cm to 115 cm of thickness at breast height. Measuring of the mean diameter and double thickness of bark was conducted by section method. In total, 4,647 diameters and bark thicknesses were measured in different relative lengths of stems or in average 10.6 measurements per one stem. Results: As an optimal model for the evaluation of double thickness of the bark of Norway spruce depending on mean diameter of roundwood the function with designated determination coefficient of 0.7142 was selected. The obtained results have confirmed the previously defined relations of investigate characteristics, which are as following: a) with the increase of mean diameter of roundwood (section) double bark thickness is increased from 9.26 mm (thickness class 12.5 cm) to 31.65 mm (thickness class 92.5 cm); b) with the increase of mean diameter of roundwood the share of bark in its volume decreased from 14.26% (thickness class 12.5 cm) to 6.73% (thickness class 92.5 cm). Conclusions: By the actual method of estimating bark thickness or the share of bark in the volume of roundwood of Norway spruce in the forestry of the Federation of Bosnia and Herzegovina a significant error was created which increases with the increase of mean diameter. The obtained results point to the necessity of investigation of these bark characteristics in Bosnia and Herzegovina and represent an inevitable starting point for making adequate tables of bark thickness and its percentage share in the volume of roundwood of Norway spruce.

Background Lung cancer is the leading cause of cancer-related death worldwide, with 5-year overall survival less than 15%. Therefore, it is essential to find biomarkers for early detection and prognosis. Aberrant DNA methylation is a common feature of human cancers and its utility is already recognized in cancer management. The aim of this study was to explore the diagnostic and prognostic value of the promoter methylation status of the ASC/TMS1/PYCARD and MyD88 genes, key adaptor molecules in the activation of the innate immune response and apoptosis pathways. Methods A total of 50 non-small cell lung cancer (NSCLC) patients were enrolled in the study. Methylation of bisulphite converted DNA was quantified by pyrosequencing in fresh frozen malignant tissues and adjacent non-malignant tissues. Associations between methylation and lung function, tumor grade and overall survival were evaluated using receiver-operating characteristics (ROC) analysis and statistical tests of hypothesis. Results Methylation level of tested genes is generally low but significantly decreased in tumor tissues (ASC/TMS1/PYCARD, P<0.0001; MyD88, P<0.0002), which correlates with increased protein expression. Three CpG sites were identified as promising diagnostic marker candidates; CpG11 (-63 position) in ASC/TMS1/PYCARD and CpG1 (-253 position) and 2 (-265 position) in MyD88. The association study showed that the methylation status of the ASC/TMS1 CpG4 site (-34 position) in malignant and non-malignant tissues is associated with the overall survival (P=0.019) and the methylation status of CpG8 site (-92 position) is associated with TNM-stage (P=0.011). Conclusions The methylation status of the ASC/TMS1/PYCARD and MyD88 promoters are promising prognostic biomarker candidates. However, presented results should be considered as a preliminary and should be confirmed on the larger number of the samples.

Chronic obstructive pulmonary disease (COPD) is a chronic disease characterized by a progressive decline in lung function due to airflow limitation, mainly related to IL-1β-induced inflammation. We have hypothesized that single nucleotide polymorphisms (SNPs) in NLRP genes, coding for key regulators of IL-1β, are associated with pathogenesis and clinical phenotypes of COPD. We recruited 704 COPD individuals and 1238 healthy controls for this study. Twenty non-synonymous SNPs in 10 different NLRP genes were genotyped. Genetic associations were estimated using logistic regression, adjusting for age, gender, and smoking history. The impact of genotypes on patients’ overall survival was analyzed with the Kaplan–Meier method with the log-rank test. Serum IL-1β concentration was determined by high sensitivity assay and expression analysis was done by RT-PCR. Decreased lung function, measured by a forced expiratory volume in 1 s (FEV1% predicted), was significantly associated with the minor allele genotypes (AT + TT) of NLRP1 rs12150220 (p = 0.0002). The same rs12150220 genotypes exhibited a higher level of serum IL-1β compared to the AA genotype (p = 0.027) in COPD patients. NLRP8 rs306481 minor allele genotypes (AG + AA) were more common in the Global Initiative for Chronic Obstructive Lung Disease (GOLD) definition of group A (p = 0.0083). Polymorphisms in NLRP1 (rs12150220; OR = 0.55, p = 0.03) and NLRP4 (rs12462372; OR = 0.36, p = 0.03) were only nominally associated with COPD risk. In conclusion, coding polymorphisms in NLRP1 rs12150220 show an association with COPD disease severity, indicating that the fine-tuning of the NLRP1 inflammasome could be important in maintaining lung tissue integrity and treating the chronic inflammation of airways.

Chainsaw is the main tool for work in a phase of wood felling and processing of forest harvesting in BiH. The aim of the research was to determine fuel and lubricant consumption of the chainsaws Husqvarna 365 and Dolmar PS – 7310 in the phase of felling and processing of wood assortments at the area of P.J. Forest Office „Zavidovići“, forest compartment 203. The volumetric method was applied for the measurement of fuel and lubricants consumption, with precise determination of fuel and lubricant quantity in chainsaw tanks. In total 140 trees of the sessile oak were felled (70 trees with chainsaw Husqvarna 365 and 70 trees with chainsaw Dolmar PS – 7310) during the research. The diameter at the breast height of felled trees ranged from 15 to 84 cm, while tree height ranged from 10,3 to 37,2 m. The total volume of processed assortments was 180,11 m3. The average fuel consumption per m3 processed assortments was 0,306 L/m3 for trees felled by chainsaw Husqvarna 365 which is for 0,042 L/m3 larger in comparison to chainsaw Dolmar PS – 7310 with average fuel consumption of 0,264 L/m3. The average lubricant consumption per m3 processed assortments was 0,102 L/m3 for trees felled by chainsaw Husqvarna 365 which is for 0,012 L/m3 larger in comparison to chainsaw Dolmar PS – 7310 with average lubricant consumption of 0,09 L/m3. Considering conducted research it can be assumed that there are differences in fuel and lubricant consumption between this two type of chainsaws for considered working conditions, but differences are not statistically significant.

In this article is presented the second part of data on selected new and noteworthy taxa for a flora of Serbia resulting from the ongoing process of examination and revision of the Herbarium collection of the Institute for Nature Conservation of the Vojvodina province (PZZP). These data are including 22 species, 14 subspecies, 1 variety, 1 form, 1 infraspecific taxon with indetermined taxonomical status [stat. indet.] and one nothospecies belonging to 11 genera (Leontodon L., Lepidium L., Leucanthemella Tzvelev, Leucanthemum Mill., Leucojum Mill., Limonium Mill., Limosella L., Linaria Mill., Lindernia All., Linum L. and Scorzoneroides Moench). One subspecies (Linum capitatum subsp. serrulatum), one nothospecies (Linaria ×oligotricha) and 3 taxa on the different infraspecific levels are new for a flora of Serbia.

Introduction. Parkinson?s disease is the second most common neurodegenerative disease with as many as 50-70% of patients experiencing psychotic symptoms during the course of the illness. Our aim was to provide an evidence-based review on the etiology, prevalence and management of psychotic symptoms in Parkinson?s disease. Material and Methods. We used references from the ?Medline? database published from 1999 to 2019. Results. The most common psychotic symptoms in Parkinson?s disease are visual hallucinations, which occur in 25-30% of patients, acoustic hallucinations in about 20%, and delusions in around 5% of these patients. The etiology of psychotic symptoms is not fully clarified, but researchers suggest a complex interrelationship of factors associated with the disease itself and the factors associated with antiparkinsonian medications. After exclusion of other etiologic causes of psychotic symptoms, it is necessary to revise the type and dose of antiparkinsonian drugs. Although pimavanserin has recently been approved by the United States Food and Drug Administration, the current treatment of choice for psychotic symptoms in Parkinson?s disease is still quetiapine. Only patients who do not tolerate or do not respond to quetiapine are treated with clozapine, which has been proven more effective, but with significant side effects. Conclusion. Timely diagnosis and adequate treatment of psychotic symptoms in Parkinson?s disease are essential, because they dramatically affect the quality of life of patients and their families. Therefore, it is necessary to establish more effective tools for screening and treatment of psychotic symptoms in Parkinson?s disease.