The aim was to study the relationship between the isoflavone content in different soybean cultivars and in the soymilk. Seven cultivars of soybean seeds from two locations with different levels of isoflavones were processed to soymilk. The content of total and individual isoflavones was determined by high performance liquid chromatography. The total phenolic content, oil and protein content in soybean cultivars were also determined. Significant differences in the content of individual isoflavones were observed within the soybean cultivars. The strong positive correlations were observed between total isoflavone content in soybean seeds and in soymilk. The total phenolic content in soybean cultivars ranged from 83 to 143.4 mg GAE/100g of soybean. The total isoflavone content in soybean seeds ranged from 71.2 to 133.8 mg/100g of soybean which comprise from 76.7 to 98.8% of the total phenols. In soymilks, total isoflavone content ranged from 16.1 to 61.0 mg/kg of soymilk. The most abundant isoflavone in soybean seeds was genistein while in soymilk it was genistin. There was statistically significant difference (p < 0.05) among two locations in total and individual isoflavone contents.
Summary The isoflavone content and antioxidant properties of five Croatian soybean seed cultivars from two locations were analysed. The content of total and individual isofavones was determined by high performance liquid chromatography. For determination of antioxidant properties scavenging capacity on DPPH radicals has been applied. The total phenolic content, oil and protein content in soybean cultivars were also determined. Significant differences in the content of individual isoflavones were observed within the soybean cultivars. The total phenol content in soybean cultivars ranged from 87.2 to 216.3 mg GAE/100g of soybean. The total isoflavone content in soybean seeds ranged from 80.7 to 213.6 mg/100g of soybean. The most abundant isoflavone in soybean seeds was genistein. There was statistically significant difference (p < 0.05) among two locations in total and individual isoflavone contents. The highest contents of total isoflavones were found in cultivar "os55-95". Conversely, cultivars poor in isoflavones also showed low levels of DPPH-radical scavenging activity.
During the war in Bosnia and Herzegovina a large number of chemicals had been released into the environment. This research was conducted to evaluate the health safety of honey (meadow, chestnut, acacia, amphorae and honeydew) produced at 18 different locations in the region of Una-Sana Canton in the northwestern part of Bosnia and Herzegovina. The determination of the pesticides, heavy metals, radioactive elements and antibiotic residues in 46 honey samples were done. The content of pesticides and heavy metals (Pb, Cd, As) were not found and the concentration of Cu, Fe, Zn in all honey samples was in accordance with EU standards. The statistically significant differences (ANOVA) in honey samples were found according to concentration of Cu (0.09 - 0.18 mg/kg), Fe (0.29 - 0.92 mg/kg) and Zn (0.18 - 1.38 mg/kg). The radionuclide content was determined by means of low-level (<5 Bq/kg), using high-resolution gamma-spectroscopy (137Cs). Antibiotics residues were carried out by disc plate method of microbiological antibiotic assay and all honey samples was negative. The results of this work indicate that this area is not polluted and is suitable for development of beekeeping.
During the war in Bosnia and Herzegovina a large number of chemicals were released into the environment. This research was conducted to evaluate the health safety of honey (meadow, chestnut, acacia, amphorae, and honeydew) produced at 18 different locations in the region of Una-Sana Canton in the northwestern part of Bosnia and Herzegovina. We determined the pesticides, heavy metals, radioactive elements, and antibiotic residues in 46 honey samples. The content of pesticides and heavy metals (Pb, Cd, As) were not found, and the concentration of Cu, Fe, Zn in all honey samples was in accordance with EU standards. Statistically significant differences (ANOVA) in honey samples were found according to concentrations of Cu (0.09-0.18 mg/kg), Fe (0.29-0.92 mg/kg), and Zn (0.18-1.38 mg/kg). The radionuclide content was determined by means of low-level (<5 Bq/kg), using high-resolution gamma-spectroscopy ( 137 Cs). Antibiotic residues were carried out by disc plate method of microbiological antibiotic assay and all honey samples were negative. The results of this work indicate that this area is not polluted and is suitable for the development of beekeeping.
Una-Sana Canton (USC) has a large forest areas where chestnut Castanea sativa Mill. grows as a self-sprout tree. The aim of this paper was to determine the morphological characteristics of chestnut fruits from four self-sprout locations (Bužim, Bosanska Krupa, Cazin and Velika Kladusa), and plantation where the domestic tree is grafted with Italian Marroni. Number of fruits/kg, the useless fruit, the percentage of kernel, diameter, width, height and weight of fruits were determined. The number of fruit/kg ranged from 160-222.5, percentage of useless fruit varied between 0.88-6.7%. Percentage of kernel ranged from 78.5 to 87.3%. According to the diameter classification, width, height and weight of fruits, chestnuts fruit from USC enters the category of the smallest fruits of the Mediterranean area. Statistical significant differences (p≤0.01) in the number of fruits/kg and weight of fruits was found between locations, as well in the width of the fruit (p≤0.05), while there is no difference in the diameter and height of the fruit. For grafted chestnut, all the characteristics provided better quality.
Chestnut has been a staple food since ancient times and after being set aside for several centuries, it has finally regained appreciation within the group of patisserie connoisseurs and has been expanded among health conscious consumers. Being relatively cheap in a modern agricultural practice, a chestnut deserves a better appreciation as a source of valuable nutrients and in terms of its composition and properties which positively affect human health when included in various modern foods. Also, it contains a very large amount of vitamin C, considerable vitamin B 6 , thiamin, folate and riboflavin, large amounts of manganese, potassium, copper, phosphorus, magnesium and iron, a small amount of valuable unsaturated fatty acids and 40% of its dry matter is composed of non-resistant and resistant starch. Chestnut is gluten-free and that makes it useful as a food for celiac patients who suffer from gluten intolerance manifested in stomach discomfort and diarrhea, fatigue and many other symptoms. On the other hand, resistant starch, which survives the small intestines and decomposes in the rectum, serves as a bulking agent to provide a feeling of satiety and it transfers sugar to the blood via a sort of controlled-released mechanism. Results from investigations on the health benefits of chestnuts in modern foods in general and the effects on the well-being of celiac patients in particular are reviewed: patient's comfort, satiety, and blood sugar levels. The effect of storage conditions and the chestnut cultivar on starch digestibility are studied in a two-month period. A decrease of up to 30% regarding resistant starch composition is observed for some cultivars when stored at 20°C as opposed to storage at -18°C. This study shows that different storage conditions affect the content of non-resistant and resistant starch.
The results of a two-years investigation of the efficacy of fungicides used for seed treatments of the winter wheat variety Zlatka against T. tritici are presented. The following fungicides designed for seed disinfection were used: Dividend 030 FS, Vitavax 200 FF, Raxil 060 FS, Panoctine 350 IS. All of the tested fungicides were found to be highly effective, the top efficacy being archived by Dividend 030 FS, followed by Vitavax 200 FF (99.99%), Raxil 060 FS (99.98%), and Panoctine 350 (99.96%). Such high efficacy shows that it would be desirable to increase the use of these wheat seed disinfectants in North-Western Bosnia. Wheat yields were found to increase 15.5 - 26.3% in 2003 and 15.6 - 28.9% in 2004, compared to control. The differences in yields, in relation to control, were statistically significant, while the effect of fungicide treatments on weight per thousand kernels and hectolitre mass of seeds was not statistically significant.
From the aspects of fish research the Una Sana Canton (henceforth USK) waters belong to the less tested areas of Bosnia and Herzegovina. Taking into the consideration this fact the quality and quantity of Una river and some tributarie, Klokot and Krusnica, have been tested. Therefore, the morphometric and meristic characteristic as well as the quality of fish meat have been analysed. Fish samples were taken from tree locations of the river flow (river-head, midlle flow and river-bed) and also from fish farming. The research was carried out in spring-summer 2002. The aim of this study was to show the quality of fish meat from natural habit (three locations) and fish farming and to establish eventually significant differences in quality according to place of trout and locations of catch. However considering the most presented Salmonidae and Thymallidae family located in Una´s tributaries, we have analysed the meat quality of Brown Trout (Salmo truta m. fario), Californien Trout (Salmo gairdneri) and European Grayling (Thymallus thymallus). The following chemical parameters have been analysed: proteins, carbohydrates, fats, water, ash, minarals.
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