Abstract This study aimed to investigate the effects of dietary supplementation with modified-hen-egg-yolk on plasma lipids and lipoprotein profiles in rats. During the four-week-experiment, 64 Wistar rats were divided into four groups of 16 (eight of both sexes), and fed commercial rat food (group C); food containing 70% commercial rat mixture and 30% freshly cooked egg yolk originating from laying hen eggs fed with 3% fish oil (group F); 3% palm olein (group P), or 3% lard (group L). The cooked egg yolk in the rat diet affected the concentrations of plasma total and LDL-cholesterol in males of the P and L groups. Cholesterol and total fat in the diet did not have a hypercholesterolemic effect on their own, but when in combination with fatty acid composition, they could contribute to an increase in plasma total and LDL cholesterol concentrations in rats. HDL-cholesterol was the most resilient plasma lipoprotein of rats to dietary treatments in our experiment. Compared to the control group, the addition of hen egg yolk to the rat diet regardless of its quality, adversely affected the values of HDL-C/TC and HDL-C/LDL-C in both males and females.

The research was performed on 40 chicken broilers of Cobb provenience to investigate the effect of dietary black cumin seed oil on some blood biochemical parameters in chicken broilers. 40 chicken broilers who were included in the 42 days lasting experiment were divided into two groups, each one of 20 individuals. Group K was the control – with no added oil in feed mixture and the second group P was the experimental one – chickens were fed with 0,025 g of p.o. administered black cumin seed oil. Feed and water supplies were ad libitum . Microclimate conditions (light, temperature and airflow) were maintained according to the technological procedure. Blood samples were taken from the wing vein of all animals from both groups at age of 25, 32 and 39 days. The following blood serum biochemical parameters were determined: total lipids, triglycerides, total cholesterol, albumen and calcium. Dietary black cumin seed oil significantly (P<0.05) affected serum lipid components, particularly total lipids, but not albumen and calcium in chicken broilers. Possible reason for these findings could be dietary fat component as well as fatty-acid composition of added black cumin seed oil. Key words: black cumin seed oil, chicken broilers, blood serum biochemical parameters, fatty acid composition of oil

The aim of this study was to research the impact of a diet supplemented with egg yolks of modified content, having in mind the type of fat added to the laying hens diet, on the brain lipids and their fatty acid composition in rats. During four weeks of the experiment, 64 Wistar rats, divided into four groups of 16 animals each (eight animals of both sexes), were fed the commercial rat feed (group C), or the feed that contained 70% of the commercial rat feed and 30% of freshly boiled yolks from the eggs originating from laying hens fed with 3% fish oil (group F), 3% palm olein (group P) or 3% lard (group L). Concentration and content of total lipids and total cholesterol, as well as the fatty-acid composition of the total brain lipids were determined in the lipid extracts of the rats brains. Under unfavourable conditions, which in our case could be high dietary intake of the total fat due to egg yolk addition, the amount of total fat in the brain tissue or the mass of the organ itself can be changed. Applied dietary treatments could also influence the level of de novo synthesis of total cholesterol in the rat brain. High dietary fat intake, as well as the fat quality regarding its fatty acid composition, appear to be able to significantly influence the fatty acid profile of the total brain lipids in adult rats, whereas the level and quality of the changes also depend on sex.

The aim of this study was to examine the effects of diets supplemented with egg yolks of modified composition on the fatty-acid composition and lipid content in rat’s liver. During four weeks of the experiment 64 Wistar rats were divided into four groups of 16 individuals each (eight individuals of both sexes) and fed a commercial feed mixture for rats (group C) or diet containing 70% commercial mixture for rats and 30% freshly cooked egg yolks from laying hens fed diets with 3% fish oil (group F), 3% palm olein (group P) or 3% lard (group L). Dietary supplementation with egg yolks significantly increased the hepatic cholesterol pool in rats, regardless of the type of fat in the diet of laying hens from which the eggs originated. The content of α-linolenic acid in the liver of male rats in group P was 4-6 times higher compared to males in the other groups. Liver lipids and their fatty-acid composition differ by both, sex and dietary modified egg yolk composition in rats.

Glandulla mammae is a secondary sexual characteristic. The composition and structure of the mammary gland depends on the functional state of the gland and is affected by hormones. The mammary gland reaches its full development during gestation and it becomes functional and secretory upon delivery. Lactocytes of the mammary gland may be squamous in shape, low or high prismatic, depending on the phase of the glandular cycle. Lactocytes are affected by LTH hormones due to which they are called prolactin. The function of the mammary gland is also affected by STH, and indirectly by TTH. Examined material was taken from Central Bosnia Canton, under field conditions of veterinary practice. The assay of histological features of the mammary gland of Dubska pramenka was conducted during the dry period as well as during lactation. The total number of animals was 16, eight (8) during the dry period and eight (8) during lactation period. Following the development of histological preparations, we conducted a descriptive overview - interpretation of the histological structures. The study of histological preparations of the mammary gland of Dubska pramenka during the dry period and lactation shows that the parenchyma is well developed, more or less, with reduced intersticium. Histological appereance of the lactocytes resembles that of the perifollicular tissue, the presence of different cells is higher during the lactation - the onset of glandural cycle. Well-developed lactiferous ducts with cavity, with or without secretion, but with preserved epithelium are the characteristics of both periods. The glandural tissue is well-developed and the secretion was present regardless of the examination period.

Pramenka je ovca kombiniranih svojstava, skromna u ishrani, vrlo otporna i dobro prilago ena našim uvjetima, ali slabih produktivnih osobina jer se uzgaja u brdsko-planinskim uvjetima, nepovoljnim za intenzivniju proizvodnju sto ne hrane, zbog ega je ishrana veoma esto kvantitativno i kvalitativno nedostatna. Na pravilnost ishrane ukazuje razina njihove proizvodnje, ali i njihov metaboli ki status koji se prosu uje odre ivanjem biokemijskih parametara krvi. Biokemijski parametri krvi mogu ukazati na energetsku, proteinsku i mineralnu opskrbu organizma, a istovremeno i na zdravstveni status životinje. Stoga smo proveli ispitivanje širokog spektra biokemijskih parametara u krvi ovaca s dva lokaliteta, op ine Livno i Travnik, u ljetnom razdoblju kada su životinje bile na ispaši karakteristi nog botani kog sastava navedenih podru ja, a sve s ciljem usporedbe sa standardnim vrijednostima parametara za ovce, ali i me usobnih podataka s dva razli ita podru ja. Krv je uzorkovana iz v. jugularis i u serumu su odre ivani sljede i biokemijski parametri: glukoza, Mg, P, Ca, ukupni proteini, LDH, GGT, AST, ALT, ALKP, kolesterol, trigliceridi, urea i albumini. Važnost istraživanja je u injenici da se prou avanjem metabili kog statusa unapre uju saznanja o interakciji lokalnog uzgoja i metabolizma ovce u funkciji održivosti autohtonih pasmina.

The aim of the study was to explore the effect of lactate on insulin-stimulated glucose uptake in rats. Thirty Wistar rats, weighing 250 - 300 g. were arbitrarily divided into one of three groups (n =10): insulin (1 IU/kg) treated group, lactate (80 mg/kg), and insulin plus lactate treated groups. Blood glucose levels were measured in venous samples collected from the tail vein over 3 hour period after insulin or/and lactate administration in 30-minute intervals. To estimate the influence of lactate on insulin blood level, a total of 20 rats were divided into 4 groups (n = 5): saline, insulin, lactate, and insulin plus lactate treated group, respectively. Sixty minutes after the appropriate application of the same doses of insulin, lactate, and lactate plus insulin, as in the previous part of the experiment, plasma insulin and blood glucose levels were determined in blood samples drawn from the abdominal aorta. Lactate in combination with insulin, in comparison to insulin application alone, caused a dramatic increase in plasma insulin level (p<0,001) and more profound hypoglicaemia (p<0,001). The results of this investigation indicate that lactate application significantly increases the rate of glucose uptake from peripheral blood caused by exogenous insulin action. The possible involvement of lactate in the mechanism of enhanced glucose uptake due to insulin action after physical exercise is discussed.

The influence of dietary palm olein, in comparison to fish oil and lard, on lipid levels in egg yolk and blood plasma, the fatty acid composition of egg yolk, and various production parameters were studied. Brown Lohman laying hens (n=45) were randomly assigned into three groups of 15 birds, and treated with experimental diets with either 3% palm olein (PO), fish oil (FO) or lard (L) for 6 weeks. At the end of the experiment, feed consumption was significantly lower for hens fed the PO diet, except week 6 of the experiment. The concentration of plasma triglycerides was increased by all experimental diets, whereas there were no significant increases of plasma total lipid and total cholesterol concentrations only in the PO group. For yolk lipids a decrease in triglycerides in the FO and L groups was observed, while total cholesterol and total lipid were significantly decreased in the PO group. Feeding with the PO diet resulted in the lowest concentrations of palmitic, stearic and linoleic acid, as well as in the highest concentration of monounsaturated oleic acid in the yolk total lipid. It was concluded that the composition of yolk lipids did not closely match the concentrations of lipids observed in experimental diets or plasma. Based on the current work it seems that the PO diet modulates egg yolk lipid content best.

Knowledge of spontaneous aberrations, namely, of their frequency in non-irradiated cells is of paramount importance not only in cytogenetic research, but also in contemporary animal production. The paper deals with research on spontaneously emerged chromosomal aberrations in the peripheral blood lymphocytes in the cattle of 'Busa' breed. To obtain metaphase chromosomes the conventional method of lymphocyte cultivation was used, albeit slightly modified and adapted to the examined animals and the laboratory conditions. The research findings indicate that a certain percent of spontaneously emerged chromosomal aberrations of chromatid type (gap and break) have been found in the peripheral blood lymphocytes in the cattle of 'Busa' breed.

The aim of this study was to investigate the possibility to modification the total lipid and cholesterol level, as well as fatty acid composition of egg yolks, by supplementing diets of laying hens with different fats. The trial was conducted in two six week experiments. Experiment I was conducted on 180 Isa Brown hens assigned to two age categories: 36 months - old (O), and 27 weeks of age - young (Y) hens. Both ago categories were divided into three groups: control groups fed a diet I with no supplemented fat (OC and YC) experimental groups fed a diet II supplemented with 3.2% of palm oil (OP and YP) and experimental groups fed a diet III supplemented with 2.5% of lard (OL and YL). In Experiment II 45 Lohman Brown hens of 56 weeks of age were randomly assigned into three groups of 15 birds each and were fed with three experimental diets supplemented with either 3% fish oil (group FO), 3% palm olein (group PO) or with 3% lard (group L). The results of our trial support the thesis of constant cholesterol content in egg yolk, that was accepted by the majority of researchers, although it was possible to affect the levels only in some conditions, as for example by the age of hens in Experiment I or by feeding Lohman Brown hens with 3% of supplemented lard in Experiment II. However, the experiment proved the possibility of altering egg yolk fatty acid composition, this being a trend in actual investigations of egg yolk cholesterogenic modification.

The authors report the problem of central venous catheter appliance to the children with malignant diseases, employed for the first time in Bosnia and Herzegovina with the aim of pediatric oncologic patients treatment. During 1997 central venous catheter type Hickman was used in nine children between two and half to eleven years old (average six years and one months). The average time of catheter placement was six months, in two cases catheter were eliminated after two and three months respectively since application (spontaneous elimination and repeated septic attacks, caused bu resistant bacterial strains). Gram-positive bacteria have been isolated with eight children (Staphylococcus aureus and Staphylococcus epidermidis), and gram-negative enterobacteriaceae (Serratia marcescens, Pseudomonas aeruginosa, Klebsiella oxytocia and pneumoniae, Escherichia coli, Salmonella group C and Enterococcus faecalis) in samples taken from the catheter and hemoculture. The central venous catheter is useful in treating oncological patients, but may cause serious consequences, like local infections or septicaemia.