The purpose of this study was to examine the effect of initial periodontal therapy on the periodontal status of patients with type 2 diabetes mellitus, in correlation with glycosylated hemoglobin (HbA1c) levels. A total of 60 patients diagnosed with type 2 diabetes mellitus and periodontal disease underwent initial periodontal therapy. Relevant data on HbA1c laboratory test results and periodontal parameters were recorded at baseline and again three months later. Statistical analysis was performed using the SPSS software package (version 13.0; SPSS Inc., Chicago, IL, USA). To compare periodontal indices between the initial and follow-up examinations, the General Linear Model – Repeated Measures (GLM-RM) was used. Additional variables that could potentially influence the outcome (therapeutic option, HbA1c levels, and presence of diabetes mellitus) were included as covariates. Descriptive statistics are presented as absolute values (n), relative values (%), and as means with standard deviations or interquartile ranges. Comparisons between variables were conducted using the paired t-test or the Wilcoxon test, depending on the distribution of the data. Based on our study, it can be concluded that initial periodontal therapy in patients with type 2 diabetes mellitus, when analyzed in correlation with HbA1c levels, resulted in a reduction in clinical periodontal inflammatory parameters. The duration of type 2 diabetes mellitus and HbA1c levels did not have a statistically significant effect on the periodontal indices monitored in this study.

Background: The etiology of oral ulceration is multicausal with numerous predisposing factors. Studies by various authors cite Helicobacter pylori infection as a possible cause of certain oral ulcerations. Objective: The aim of the study is to prove the presence of Helicobacter pylori in the oral cavity of patients with oral ulcerations, as well as to examine the relationship between the presence of Helicobacter pylori in the oral cavity and the development of oral ulcerations. Methods: The study included regular patients at the Department and Clinic of Oral Medicine and Periodontology, Faculty of Dental Medicine, University of Sarajevo, suffering from oral ulcerations, as well as healthy patients without oral diseases. The diagnosis of oral ulceration is based on a thorough history, clinical examination, and exclusion of other oral diseases. All patients were taken anamnestic data on the existence of digestive system diseases, and by reviewing medical documentation, a previously diagnosed digestive system disease by a gastroenterologist was recorded. A cytological smear was taken in all 80 cases, in patients with ulcerative lesions a smear was taken from the lesion and in healthy subjects from the mucous membrane of the palate, cheek and tongue. Highly sensitive and specific polymerase chain reaction (PCR) was used to determine the presence of Helicobacter pylori in the oral cavity and oral lesions. Results: The results of our study showed that there is no statistically significant difference between the presence of Helicobacter pylori in the oral cavity of patients with oral ulcerations and subjects with healthy mucosa. Conclusion: Helicobacter pylori is not a risk factor for the development of oral ulcerations and can be found on the oral mucosa as a transient pathogen.

Introduction: Developmental anomalies and inflammations are common occurrences and are often associated with certain systemic conditions, such as diabetes, allergies, and anemia, as well as with harmful habits. The use of tobacco products and alcohol is frequently linked to changes on the tongue. Objective: This study aims to present the frequency of developmental anomalies and tongue inflammations in the student population of the fourth year at the Faculty of Dentistry in Sarajevo. Materials and Methods: The study was conducted on 50 participants, fourth-year students at the Faculty of Dentistry in Sarajevo. General anamnestic data were collected through a survey, including information on harmful habits such as cigarette smoking, hookah use, and alcohol consumption. A clinical examination of the oral cavity, focusing on the tongue, was performed for each participant, along with vitro-pression, vitro-adhesion tests, and native findings on Candida albicans. Results: The most common developmental anomaly was lingua plicata (4%). The most frequent tongue inflammation in our sample was lingua geographica (14%). Among the 6% of patients with developmental anomalies, 6% had a positive family history. Conclusion: A significant etiological factor is genetic predisposition. In our sample, among 6 participants with developmental anomalies, 6% had a positive family history. The most common etiological factors for the occurrence of developmental abnormalities and tongue inflammations were harmful habits such as cigarette smoking (20%) for over 5 years (14%) and hookah smoking (12%) for over 5 years.

Objective: This comprehensive research aimed to thoroughly examine the effectiveness of a diode laser (445 nm) in combination with non-surgical treatment in patients with chronic periodontitis (CP) by evaluating a wide range of clinical and microbiological parameters. Materials and methods: Thirty-one subjects diagnosed with CP were included in this study. The total number of treated periodontal pockets was 862. The subjects were randomly assigned to group 1, which underwent scaling and root planing and laser therapy (SRP+L), and group 2, which underwent scaling and root planing (SRP) only. All respondents underwent a periodontal diagnostic protocol. The parameters plaque index (PI), gingival index (GI), bleeding on probing index (BOP), probing depth (PD), clinical attachment level (CAL), and tooth mobility (TM) were registered. Clinical periodontal measurements were performed at baseline and one and three months after therapy. Microbiological analysis was conducted on Aggregatibacter actinomycetemcomitans (Aa), Porphyromonas gingivalis (Pg), and Tannerella forsythia (Tf) using real-time polymerase chain reaction (PCR). For microbiological analysis, samples were taken at baseline, immediately after therapy, and after three months. Laser irradiation was performed immediately after SRP. Results: All clinical parameters improved statistically from baseline to three months after therapy. For all examined clinical parameters, better results were achieved in group 1 than in group 2. This study showed a more significant reduction in Pg and Tf from baseline to three months in group 1 compared to group 2. Conclusion: These results showed that the diode laser wavelength 445 nm was also usable in treating periodontal diseases as an additional method to SRP.

Background: Non-surgical periodontal therapy, including mechanical debridement and root planning, is the gold standard in the treatment of periodontal diseases. The most commonly used instruments for non-surgical therapy are sonic/ultrasonic devices and manual instruments such as curettes. Objective: This study evaluates the clinical effectiveness and patient experience of non-surgical periodontal therapy using the Vector® Paro Pro ultrasonic device, emphasizing its impact on periodontal indices and patient comfort. Methods: Fifty patients with gingivitis, periodontitis, or peri-implantitis participated. Baseline data, including Plaque Index (PI), Gingival Index (GI), Papillary Bleeding Index (PBI), Probing Depth (PD), and Clinical Attachment Loss (CAL), were recorded. Patients underwent treatment using the Vector® Paro Pro system. Evaluations were conducted at baseline, two weeks, and one month post-therapy. Statistical analyses utilized repeated measures ANOVA and paired t-tests, with a significance threshold of p<0.05. Patient experience was assessed via a questionnaire. Results: All periodontal indices showed significant improvement post-therapy. The mean PI decreased from 1.18±0.12 to 0.52±0.08 after two weeks and 0.44±0.09 after one month (p<0.001). Significant reductions were observed in PBI (from 1.68±0.10 to 0.46±0.09) and PD (>4 mm pockets: from 5.55±0.19 to 3.65±0.45; p<0.001). CAL improved significantly (from 0.80±0.18 to 0.70±0.16 after one month; p<0.001). Patient-reported outcomes revealed that 98% experienced no pain during therapy. Conclusion: The Vector® Paro Pro ultrasonic device demonstrated significant clinical improvements in periodontal health, particularly in bleeding reduction and deep pocket management. Its hydroxyapatite-enhanced fluid effectively polished root surfaces and reduced post-therapeutic sensitivity. Patients reported minimal discomfort, underscoring the device’s potential as a comfortable, efficient alternative for non-surgical periodontal therapy. Further studies with longer follow-up periods are recommended to confirm these findings.

Background: Periodontal complications are one of the common side effects associated with orthodontic therapy. Objective: This study aimed to evaluate the periodontal changes in patients before, during, and after the therapy with a fixed orthodontic appliance. Methods: Out of 38 healthy adolescents with permanent dentition who were indicated for fixed orthodontic therapy were included in this study. Patients were selected from Class I, treated by non-extraction methods, by using conventional orthodontic braces. After their examination and treatment by an orthodontist, the patients were referred to the periodontist before the placement of a fixed orthodontic appliance. The patients underwent the application of a periodontal anamnestic-diagnostic protocol, and the clinical-radiological evaluation. After a fixed orthodontic appliance was placed, the respondents were referred to the periodontist for regular mandatory check-ups, initially, after three months, and later on-after 6 months, after 1 year and after 2 years until the end of orthodontic therapy. Results: An increase in the mean value of the Plaque Index and Sulcus Bleeding Index was found at each check-up after the placement of a fixed orthodontic appliance. There is a statistically significant difference in the presence of gingival hyperplasia found by monitoring the changes after three and six months, and after one and two years following the start of orthodontic therapy. Conclusion: The assessment of periodontal changes in patients before, during and after the completion of fixed orthodontic therapy revealed that there is a strong need for mutual and close cooperation between orthodontist and periodontist during orthodontic therapy.

Amaç: İnterlökin-6’nın (IL-6) periodontal hastalık patogenezi ve periodontal doku yıkımındaki rolü geniş çapta ele alınmıştır. Çalışmanın amacı, evre IV periodontitis hastalarının tükürüklerindeki IL-6 seviyelerini değerlendirmektir. Gereç ve Yöntemler: Araştırmaya evre IV periodontitis teşhisi konulan 28 hasta ile 22 hastadan oluşan kontrol grubu dahil edildi. Hastaların tümü sistemik olarak sağlıklıydı. Tükürük örnekleri toplandı ve sondalama derinliği (SD), klinik ataşman seviyesi (KAS), papil kanama indeksi (PKİ), sondalamada kanama yüzdesi (SK %), plak indeksi (Pİ) ve diştaşı indeksini (Dİ) içeren klinik periodontal ölçümler kaydedildi. Her bir hastanın uyarılmamış tükürükleri, tükürük toplayıcı ile toplandı ve örneklerdeki IL-6 seviyesi enzim bağlı immünosorbent testi ile analiz edildi. Bulgular: Evre IV periodontitis hastalarının ortalama IL-6 değeri 22,18±5,96 pg/mL idi. Kontrol grubunun ortalama IL-6 değeri ise 2,23±2,17 pg/mL idi. Periodontitis Objective: The role of interleukin-6 (IL-6) in the pathogenesis of periodontal disease and tissue destruction at the periodontal site has been widely reported. This study aimed to evaluate the salivary IL-6 levels in patients with stage IV periodontitis. Materials and Methods: The study included 28 patients who were diagnosed with periodontitis stage IV and the control group of 22 periodontally healthy patients. All the patients were systemically healthy. Saliva samples were collected, and clinical periodontal measurements, including probing depth (PD), clinical attachment level (CAL), papilla bleeding index (PBI), the percentage of sites with bleeding on probing (BOP) %, plaque index (PI) and calculus index (CI), were recorded. The unstimulated saliva of each patient was collected by a saliva collector, and all samples were analysed using the enzyme-linked immunosorbent assay method for the detection of IL-6. Results: The mean value of salivary IL-6 in patients with periodontitis stage IV was 22.18±5.96 pg/mL. In the control group, the average measured value of IL-6 was 2.23±2.17 pg/mL. The periodontitis group had a significantly higher salivary IL-6 levels than the control group. A strong positive correlation was observed between the salivary IL-6 and clinical periodontal parameters (PD, CAL, PBI, BOP %, PI and CI) in patients with periodontitis stage IV (p<0.0001). Conclusion: We demonstrated a statistically significant relationship between periodontal parameters and salivary IL-6 in patients with periodontitis stage IV. New studies are needed to accurately establish salivary IL-6 potential as a biomarker for periodontal disease monitoring, including all stages and grades of periodontitis.

Background: Dentine hypersensitivity (DH) is a frequent clinical problem that represents a long-term painful discomfort for the patients, and for the dentists, it represents a diagnostic and therapeutic challenge. Objective: The aim of the research is to verify the effect of the treatment with diode laser SiroLaser Blue (660nm) of DH alone or in combination with different impregnating agents. Methods: Fifty patients were included in this research, separated into five groups. All the patients have been asked to define the level of dentine hypersensitivity using VAS (0-10). The first group was treated with Fluor Protector, the second group after the application of Fluor Protector has undergone irradiation with SiroLaser Blue (660nm), the third group was treated with impregnating agent Vivasens, the fourth group, after the application of impregnating agent Vivasens, has had SiroLaser Blue (660nm) irradiation. The fifth group has just been treated with SiroLaser Blue (660nm). The efficiency of the treatment was checked using VAS for every group immediately after the conducted treatment, after 7 days and 1 month. Results: Our results showed that all of the desensitizing agents used in the research alone or in combination with a diode laser (660nm) have shown a reduction of DH. The difference has been proven statistically significant in mean values by groups and examinations. Vivasens and diode laser irradiation have provided the best results in the review of mean values after the first examination and one month after the treatment of DH (p<0,05). In our research, the application of diode laser alone has not proven superior to other treatment methods that have been used in the research. Conclusion: Vivasens plus diode laser irradiation has provided the best results even after one month since the treatment of DH.

Summary Background/Aim: This study aimed to evaluate the effects of Lactobacillus reuteri lozenges as an adjunctive therapeutic agent in combination with scaling and root planing in a randomized, clinical trial of volunteers with periodontitis stage IV. Material and Methods: The study included 40 patients diagnosed with periodontitis divided into 2 groups of 20 patients by random sample method. The first group of patients used Lactobacillus reuteri lozenges after nonsurgical periodontal therapy for a period of 40 days while the second group of patients was treated with non-surgical periodontal therapy without lozenges. Periodontal clinical parameters were registered for all patients before treatment and after 40 days. Samples of saliva from patients before and 40 days after treatment were analyzed by the PCR method for pathogens Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and Prevotella intermedia. Assessments were made on day 0 before treatment for patients of both groups and after 40 days. Results: Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis show a statistically significant difference between the two study groups. Results were not statistically significant for Prevotella intermedia (P= 0.5598). Conclusions: The present study confirms the positive effects of L. reuteri lozenges after non-surgical periodontal therapy and the maintenance phase of periodontal treatment. Considering the beneficial effects of probiotics, L.reuteri could serve as a useful adjunct or maybe even as an alternative to periodontal treatment when scaling and root planing might be contraindicated or has to be postponed.

Introduction: Cigarette or hookah smoking, as well as alcohol consumption and abuse, are considered to be the most common etiological factors for the onset of oral cavity diseases, such as changes on the lips, tongue, stomatopyrosis, glossopyrosis, candidiasis. Aim: Research aims to determine the harmful effect of smoking the hookah as well as other harmful factors on the oral health of the student population of the Faculty of Dentistry in Sarajevo. Methods: The fourth, fifth, and sixth-year students of the Faculty of Dentistry in Sarajevo participated in this cross-sectional study. All of them are systemically healthy and consume some harmful habits: smoking cigarettes, alcohol consumption, and hookah. All students participated in the study voluntarily and they signed informed consent before the clinical examination. All the subjects gave an extensive medical history which recorded all the data on oral hygiene, harmful habits, manner and length of consumption; they were also given a clinical examination of the oral mucosa and the periodontium, as well as determining periodontal indices, and oral tests that are used for diagnostic purposes. The data is entered into work charts specially designed for these purposes. Results: The results are statistically processed in the SPSS Statistics 21.0 program and are discussed along with the results of other authors, published in relevant databases. Conclusion: The conclusion consists of important facts that originate from the results and the discussion.