New approach in food safety and hygiene legislation gives the central role to HACCP for all food business operators, with an exception of primary production. Slowly ongoing transposition of the EU food safety legislation into regulatory framework of Bosnia and Herzegovina (B&H) also puts HACCP as an obligatory system for food operators. However, the implementation of HACCP is still on relatively low level, with some misunderstandings. The reasons are commonly found in lack of quality plans for implementation and control of implementation of the adopted regulation. As far there was not any systematic research of food operators' approaches and positions toward HACCP as obligatory and, according to some researches, costly system. The aim of the study was to find familiarity of B&H food business operators with impact of new regulatory framework on their business. The research was done by survey method in 18 companies. the surveyed operators, depending on their profile, had highly different opinions regarding costs pertaining to HACCP, administrative burden of HACCP implementation, and overall aims and benefits of its implementation. The results of the study pointed out necessity of further training and education of food business operators in B&H regarding food hygiene legislation and its purposes.

Wild boars are present in the country as game freely living in the nature, but in a controlled breeding as well, for the purposes of training hunting dogs. Monitoring the health status of animals is important both from the clinical and epidemiological stand points. Studies of the health status of wildlife in Bosnia and Herzegovina are very rare, and we do not know any earlier information on the study of biochemical parameters of blood of these animals in our country. Our aim was not only to establish starting results but also to affirm similar research. The study was conducted on two farms with 23 and 16 young wild boars. The animals were in good health condition and housed in a controlled, enclosed part of the natural habitat. After fixing and restraint of animals, blood was sampled from V. auricularis magna into vacutainers, without anticoagulant. The serum values of the following biochemical parameters were determined: potassium, sodium, calcium, urea, creatinine, alanine aminotransferase and alkaline phosphatase. The differences as regards activities of certain parameters were evidenced among farms as well as in comparison with the results obtained by other authors. These and other studies aiming the protection of game health are important steps toward understanding the pathology and the pathological physiology of these animals. This research may be considered a pioneering step in that direction for Bosnia and Herzegovina and should be continued in order to reach the most credible results with an aim of improving the protection of health of wild but also of the domestic animals and humans.

Ionising radiation is known to cause chromosomal instability, which is observed as increased frequency of chromosomal aberration and micronuclei. These are listed as reliable criteria in biological dosimetry. Numerous experiments conducted on both animal and plant models demonstrated that increase in radiation dosage is followed by increased mutation frequency, and that mutations occur even at the lowest exposure. We used horse blood in vitro irradiated by low doses of ionizing radiation. Cultivation of peripheral blood lymphocytes and micronucleus test were used as biomarkers of genetic damage. The observed aberrations were recorded and classified in accordance with the International System of Cytogenetic Nomenclature. Micronuclei were identified on the basis of criteria proposed by Fenech et al . (8). Analysis of chromosomal aberration showed increased frequency of aberrations in blood cultures exposed to 0,1 Gy and 0,2 Gy compared to the controls. Microscopic analysis of chromosomal damage in in vitro micronucleus test revealed that the applied radiation dose induced micronuclei while no binucleated cells with micronuclei were found in lymphocytes that were not irradiated. In this paper we analysed the influence of low dose ionising radiation on frequency of chromosomal aberration and micronuclei in horse lymphocytes following in vitro exposure to X-rays (0,1 Gy and 0,2 Gy). Key words : chromosomal aberrations, micronuclei, ionising radiation, horse lymphocytes