3 . Anthropogenic emissions of heavy metals affect the ongoing pollution of the Tuzla area. Cultivated plants acquire large amounts of heavy metals and therefore there is a real possibility of their involvement in the food chain. Goals: The basic objective of this paper is to determine the content and dynamics of heavy metals: cadmium (Cd), chromium (Cr), copper (Cu) and zinc (Zn) in the fruit and leaves of plum (Prunus domestica L.) in the Tuzla area in order to obtain information about their impact on the environment. Materials and methods: Research consisted of fieldwork and laboratory analysis. Plant material was sampled at nine sites in the Tuzla area and prepared for chemical analysis of heavy metals. The content of heavy metals: chromium (Cr), cadmium (Cd), copper (Cu), zinc (Zn), in solutions of plant material samples was determined by atomic absorption spectrophotometry (AAS method), with the instrument "Perkin- Elmer" 3110 and graphite cuvette "Perkin-Elmer" HGA-440. Determination of heavy metals was carried out according to ASTM-E 1812-96 standard. Results: The determined values of cadmium and copper content in plum leaves were higher than the natural content for plants in non-polluted environments in most localities. Cadmium concentration in the fruit of plum on most sites exceeded natural values of 0.8 mg/kg. The content of zinc in the fruit of plum at all locations was within the limits of the average value. In plum leaves the identified concentrations at the site of Donji Bistarac were 2.5 times higher than the average value which is 30 mg/kg. The highest concentration of chromium in leaves of plum was found at the site Donji Bistarac (2.25 mg/kg), and lowest at the site Donji Pasci. Plum fruit has a much smaller amount of the mentioned metal than a leaf, except at the site Donji Pasci where the determined values were 3 times higher than the average. Conclusion: The highest concentrations of heavy metals were found on sites that are located near industrial plants. Therefore, in the industrial-urban areas there should be provided continuous monitoring of heavy metals content in order to produce healthy food and improve the quality of life of people.
Rosaceae is a numerous family including 115 genera and more then 3000 species spread throughout the world. The genus Fragaria (strawberry) embraces perennial herbaceous plants. The existing material was collected during different seasons and phenophases of the species at topographically and environmentally different locations around Tuzla. Parallel morphological analyses revealed that the species Fragaria vesca L. shows a high degree of variability for the traits analysed. Therefore, a detailed understanding of the morphological variability and ecological differentiation of the species population is an essential condition for the assessment of the biological potential and evolutionary status of the test herb.
The aim of this study was to compare total phenols and sulfur content in ramsons and two garlic species, autumn- and spring-garlic. Harvesting time for ramsons was May and for garlics was June. Total phenol content was determined by the Singleton-Rossi method, which is based on phenol oxidation using Folin-Ciocalteu reagent and spectrophotometric quantification of reduced blue-colored products. For total sulfur analysis, all sulfur molecular species were oxidised to the stable sulfate form, which was quantified by ion chromatography (HPIC). The quantity of phenolic compounds (mg phenols/g fresh sample) was the highest for the leaves of autumn-garlic (1.97mg/g), followed by leaves of spring-garlic (1.49mg/g) and ramsons (1.28mg/g). A lower phenol content was found in the bulbs: spring-garlic bulb (0.80mg/g), autumn-garlic bulb (0.48mg/g) and ramson bulb (0.46mg/g). The highest sulfur level (mg sulfur/g fresh sample) was found in spring-garlic leaf (1.10mg/g) while the quantity of sulfur for other samples were: ramsons bulb (0.93mg/g), ramsons leaf (0.74mg/g), spring-garlic bulb (0.70mg/g), autumn-garlic leaf (0.66mg/g) and autumn-garlic bulb (0.63mg/g). Levels of sulfur compounds and total phenol content in the bulbs and leaves correlated with the age of the plant. Garlic leaves can be used as a significant source of organosulfur compounds for middle to late spring.
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