The aim of this study was to investigate liver function in dairy sheep during summer and consequences made by heat stress. The experiment was performed on 117 Pramenka sheep at two different locations (Travnik and Livno) during summer grazing period (July, August and September). Levels of serum enzymes were determined for alanine aminotransferase (ALT), aspartate aminotransferase (AST)), gamma-glutamyl transferase (GGT), alkaline phosphatase (AP) and lactate dehydrogenase (LDH). Enzyme activities resulted in significant (P<0.05) variation except GCT and a moderate increase followed by dcrease in activity, which is likely to be correlated with the energy balance of animals. Values of serum profile above reference range may indicate intense metabolic processesasaliver response toa negative energy balance. The results of this research couldbeused for the determination ofhealth status.

espanolEl objetivo del presente estudio fue determinar el efecto de la duracion y la temperatura de almacena-miento en los globulos rojos y los globulos blancos, asi como el volumen de celulas empaquetadas en mues-tras de sangre de un control deslizante de orejas rojas de adultos sanos. Se recogieron muestras de sangre de seis controles deslizantes de orejas rojas adultos. Las muestras de sangre se analizaron inmediatamente despues del muestreo para obtener el valor de referencia (BV) del recuento de globulos rojos, el recuento de globulos blancos y el volumen de celulas empaquetadas. Posteriormente, la sangre se almaceno a 4°C y los analisis hematologicos exactos se realizaron despues de 24 h, 48 h y 72 h. Nuestra investigacion sobre muestras de sangre de resbaladero de orejas rojas mostro el mismo nivel de estabilidad para el recuento de globulos rojos y globulos rojos durante 72 horas de almacenamiento a 4°C y para PCV durante 48 horas. El manejo de las muestras de sangre, asi como la duracion del almacenamiento, pueden influir significa-tivamente en los resultados de las determinaciones hematologicas. En consecuencia, los resultados de las determinaciones hematologicas de muestras de sangre almacenadas o manipuladas incorrectamente pueden dar resultados enganosos. EnglishThe aim of present study was to determine the effect of storage duration and temperature on the red blood cells and white blood cells, as well as packed cell volume in blood samples from healthy adult red-eared slider. Blood samples were collected from six adult red-eared sliders. Blood samples were analyzed immediately after sampling to obtain the baseline value (BV) of red blood cells count, white blood cell count and packed cell volume. Afterwards the blood was stored at 4 C and the exact hematological analyses were performed after 24h, 48h and 72h. Our research on red-eared slider blood samples showed same level of stability for RBC and WBC count during 72 hours of storage at 4°C and for PCV during 48 hours. Handling of blood samples, as well as duration of storage can significantly influence the results of hematological de-terminations. Consequently, results of hematological determinations of improperly stored or handled blood samples can yield misleading results.

Stray dogs are the ones not microchipped, which live across the streets and other public surfaces unattended, and so represent a serious public-health problem. Lack of human support for the stray dogs causes a range of problems from territorial status to ensuring food. Such conditions force them to activate a self-preservation mechanism and return to natural behavioral patterns. Regarding the fact that several thousands of stray dogs were recorded in observed regions of Bosnia and Herzegovina in the period from 2008 – 2009, it was obvious that such conditions could endanger the health of both humans and domestic animals. The problem in Bosnia and Herzegovina attempted to be solved using various approaches during the period from 1996 – 2009. Some solutions were: using hygiene services of public utility companies in some places, or establishing dog shelters. The third solution for the problem was the employment of hunting associations and their active participation for reducing the number of stray dogs. Minimization of large number of stray dogs required number of measures and activities such as aplicable legal framework, education for dog owners, neutering of dogs, building shelters, euthanasia of ill and agressive dogs and permanent hosting of dogs.

The aim of this study was to determine for how long cows´ blood can be stored without significant alterations of some of its hematological parameters during different storage temperatures. We examined alterations of selected parameters (red blood cells count, white blood cells count, hemoglobin concentration and packed cell volume) when blood samples were stored for 72 hours on fridge temperature (4 oC) and ambient temperature (18 °C). Samples were taken from six clinically healthy Holstein-Friesian cows. The results indicate that the length of storage time as well as the temperature of storage may affect some investigated hematological parameters. Differences were considered statistically significant whereas p<0,05. Key words: cows, hematology, storage temperature, duration of storage

The study was aimed at determining plasma concentrations of albumin and total protein (TP) in 480 dairy Holstein-Friesian breed cows on different localities in Bosnia and Herzegovina and in different seasons (diet conditions). The samples were taken from the cows in lactation, postpartum period within 15 days from calving, and the dry period. Seasonal variations affected both investigated parameters. Differences among localities were determined during summer for TP concentrations and during winter for albumin concentrations. Differences were considered statistically significant at p<0,05. Results show significant influence of the diet conditions on plasma concentrations of examined parameters so the conclusion is their constant monitoring during the production period is required. Key words: dairy cows, albumin, total protein

The research was conducted on 240 clinically healthy Holstein-Friesian cows in various stages of the reproductive cycle (lactation, dry period, and up to 15 days from calving). Sampling was taken during the summer and winter periods. Concentrations of calcium, phosphorus and magnesium in blood plasma were determined by using the appropriate method with the “Beckmann DU-64 UV/VIS” spectrophotometer. Significant differences were determined in the values of concentrations of the examined minerals during various stages of the reproductive cycle but in dependence on the season of sample taking.

Background: Hematology results are often influenced by the time between blood sampling and measurement, as well as storage conditions (e.g., temperature and time) during sample delivery between laboratories may further affect the resulting data. Hematological changes may occur in the measured parameters as a consequence of delayed analysis and may complicate interpretation of the data. Delayed analysis of blood samples may be caused by restricted access to laboratories. Blood samples collected from turkeys in remote locations (farms) often wait for laboratory processing. Objectives: The aim of this study was to investigate changes that occur in the packed cell volume (PCV), hemoglobin concentration, red blood cell (RBC) counts and mean corpuscular values (MCV) of turkey blood samples due to storage, including: blood samples stored at refrigerator (4°C), laboratory (24°C) and water bath (33°C) temperatures across a storage period of 72 h. Methods: Blood samples were collected from 25 adult turkeys (British United Turkey 600 hybrids – BUT 600). Hematological determinations were carried out on the blood samples immediately upon collection to obtain the baseline value (BV) and thereafter at specific time intervals across the 72-h duration of storage (DOS). Results: Results showed that for the samples stored at 4°C, there were no significant changes at p<0.05 level from the BV in the PCV, hemoglobin concentration and RBC counts, all through the 72-h DOS, but significantly increased values (p<0.05) were obtained when samples stored at 24°C and 33°C. Conclusion: Based on our research results we concluded following: blood samples obtained from turkey stored up to 72 hours at 4°C provides legitimate results for PCV, Hgb concentration and RBC count. MCV value is reliable if blood sample was stored up to 30 hours.

The study was aimed at determining levels of alanin aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) in the plasma of 229 dairy Holstein-Friesian breed cows in different stages of the re¬production cycle and regarding the seasonal changes (diet conditions). Cows were divided into the groups: lactation (Group A), postpartal period within 15 days from calving (Group B) and dry period (Group C). Seasonal variations affected AST activity within Groups A and B, while Group C showed no seasonal influence. Differences between Groups A and B were determined during winter, as well as among Groups A and C during the same season. The highest activity of ALT was measured in cows from Group A during the winter season. ALP activity showed no statistical difference among groups, though statistical significance was noted within seasonal changes. The activity of LDH showed no statistical significance within Group C with regard to the seasonal changes. Statistically significant difference was found between cows of Group A and C during the winter season. Differences were considered statistically significant when p<0,05. Research results showed a significant influence of milk production and dry period on the activities of measured enzymes in the blood plasma so we can conclude that there is a need for constant monitoring of the described parameters during the production period. Key words: dairy cows, ALT, AST, ALT, LDH