UDK 582.632.2:581.9(497.6) In this paper is given an overview of the rare occurrence of beech with oaklike bark (Fagus sylvatica var. quercodies) in Bosnia and Herzegovina, which is recorded for the first time by Karlo Malý in 1934. In addition to basic information about the distribution of this taxon we gave a detailed description of a one its remarkable recent tree from Kreševo area. These unusual phenomena induce scientific interest and require special protection and conservation actions.

Abstract The Buffelgrass (Cenchrus ciliaris L., Poaceae) is one of the most important pasturage grasses due to its high productivity and good forage qualities. This species possess a high adaptability to bioclimatic constraints of arid zones and may be used for the restoration of degraded arid ecosystems. Tunisian populations present three ploidy levels (4x, 5x and 6x) with a basic chromosome number x=9. This study reported for the first time the distribution of the ribosomal genes (rRNA) for pentaploid and hexaploid cytotypes of Cenchrus ciliaris. Molecular cytogenetic study using double fluorescence in situ hybridization has shown that the two rDNA families, 5S and 18S-5.8S-26S (18S), displayed intraspecific variation in number of loci among different ploidy levels. Each ploidy level was characterized by specific number of both 5S and 18S rDNA loci (two loci in tetraploid, five in pentaploid and six in hexaploid level). For three studied cytotypes (4x, 5x and 6x) all 5S rDNA loci were localized on the subcentromeric region of chromosomes, while 18S loci were situated on the telomeric region of short chromosome arms. Data of the FISH experiments show proportional increase of ribosomal loci number during polyploidization processes.

Habitati na serpentinskim substratima predstavljaju nepovoljne uvjete za razvoj biljaka. Karakteriziraju se sa malim brojem vrsta, ali prisustvom velikog broja endema. U ovoj studiji se po prvi put prezentira serija podataka o velicini genoma serpentinofita, njihovom hromosomskom broju, nivou ploidije, sklonosti ka supstratu, tipu životnog ciklusa i životne forme na krajnjem sjeverozapadnom dijelu serpentinskog podrucja Balkanskog poluostrva. Uzorak je obuhvatao 308 svojti iz 213 rodova, sa novim vrijednostima za 28 rodova i 99 vrsta. Prema Leitch-evim kriterijima, vise od polovine analiziranih svojti (55.63%) pripadale su grupi vrlo malih genoma, 22.19% malim, 18.75% srednjim, 3.13% velikim i samo 0.31% vrlo velikim genomima. U odnosu na sklonost ka supstratu, glavnina vrsta (171) su bile indiferentne ili fakultativne serpentinofite (103). U zavisnosti od životnog ciklusa, ~ 4% vrsta su bile jednogodisnje, a 88.31% visegodisnje, od kojih je 57% imalo vrlo male genome. Hemikriptofite su predstavljale dominantnu životnu formu (48.38%), koju slijede fanerofite (17%), hamefite (15%), terofite (9%) i geofite (9%). Iz dobivenih rezultata proizilazi da vodni stres, visoke temperature i prisustvo teskih metala u serpentinskim habitatima imaju visok selektivni pritisak i favoriziraju visegodisnje vrste sa vrlo malim genomom.Narcissus poeticus (Amaryllidaceae), fakultativna serpentinofita, je predak kultiviranih narcisa. Ovo je prva studija o N. poeticus i njegovoj rizosferi u prirodnim populacijama. Ova vrsta pokazuje toleranciju na promjene pH vrijednosti u dijapazonu od 4.64 do 7.85. Totalne koncentracije nikla, kobalta i magnezija u serpenitnskim tlima su bile vece nego u krecnjackim. Narcissus poeticus se karakterizirao vecom akumulacijom mangana, nikla i magnezija u nadzemnim dijelovima biljke. Suprotno, kobalt je imao skoro istu totalnu koncentraciju u svim dijelovima biljke. Druga neuobicajena karakteristika N. poeticus je najveci iskazani molarni odnos Ca/Mg u podzemnim dijelovima, vjerovatno zbog njegove životne forme (geofita) i ljetne dormancije. Ocito je da iako N. poeticus akumulira određene kolicine istraživanih teskih metala (Mn, Ni, Co, Fe) on se ne moze smatrati nije njihovim hiperakumulatorom.Važan dio ove studije se odnosi na varijabilnost hromosomske strukture, velicine genoma, nivoa ploidije i prisustva B-hromosoma u 13 prirodnih populacija N. poeticus koje rastu na razlicitim geoloskim supstratima i pod razlicitim okolisnim uslovima. Koristena je tehnika protocne citometrije za određivanje velicine genoma, fluorescentna in situ hibridizacija (FISH) za fizicko mapiranje rDNK, fluorohrom banding za organizaciju heterohromatina i bojenje srebrenim nitratom za utvrđivanje aktivnosti ribozomalnih gena. Organizacija ribosomalnih gena i prisustvo prirodnih triploida su u ovoj studiji saopceni po prvi put. Uoceno je prisustvo individua koje nose B-hromosome (u 9 od 13 populacija) i hromosomske translokacije. Poseban sistem B-hromosoma je predstavljen sa tri razlicita morfotipa. Najcesci submetacentricni tip pokazuje cetiri razlicita obrasca u organizaciji heterohromatina i rDNK. Bojenje s AgNO3 je pokazalo da se formirani broj nukleolusa povecava u prisustvu B-hromosoma koji nose ribosomalne gene, sto potvrđuje njihovu aktivnost. Dobiveni rezultati pokazuju da N. poeticus ima dinamican genom sa razlicitom kolicinom DNK usljed prisustva poliploidije, B-hromosoma i hromosomskih rearanžmana. Uocene promjene najvjerovatnije odražavaju odgovor genoma na razlicite okolisne uslove u kojima individue koje posjeduju B-hromosome imaju izvjesnu selektivnu prednost.

A revision of the genus Gymnospermium (Berberidaceae) in the Balkan Peninsula is carried out. Three species are recognised. Gymnospermium maloi is described as a new species from Mt. Picari in Gjirokastra district, southern Albania. It is compared with the closely related G. scipetarum which has a different habitat and distribution in central Albania and southern Montenegro. The chromosome number and karyotype features of G. maloi are provided for the first time. The chromosome formula of 2n = 2x = 14 (1 metacentric, 1 meta-submetacentric and 5 submetacentric chromosome pairs) is unusual as 2n = 16 has been reported for other members of the genus. The nuclear DNA content (2C-value) of all three species was determined. The genome size of G. maloi is 29.44 (+/- 0.47) pg, for G. scipetarum (chromosome number still unknown) 29.55 (+/- 1.35) pg, and for G. peloponnesiacum (2n = 2x = 16) 31.93 (+/- 2.38) pg. These values are the first genome size measurements for the genus. All three species are mapped and fully illustrated. A key to the European species is also presented.

Genus Artemisia is thought to have reached the Americas across the Bering Strait from Asia during the late Tertiary, but the systematic position of the South American endemic species and the migration routes towards the south have not yet been studied. We used nuclear DNA sequences to unravel the interspecific relationships among the South American Artemisia and their connections with the remaining species of the genus, as well as using fluorescent in situ hybridisation and genome size assessments to characterise this polyploid complex. Most of the species are clustered in a monophyletic clade, nested within the American endemic clade, with the exception of A. magellanica Sch. Bip., which appears segregated from the other American species and constitutes a clade together with A. biennis Willd. Fluorescent in situ hybridisation and genome size data revealed that monoploid genome size remains quite constant across ploidy levels and a proportional increase of ribosomal loci was detected, a dynamic not usually found in this genus. The results are discussed in the light of evolutionary processes which occur in plants, and plausible origins for the South American endemic species are hypothesised.

ABSTRACT This study elaborates the risogenesis induction of black currant, and possibility of its mass production by means of vegetative reproduction, with usage of ripe cuttings. Furthermore, we presented the influence of the affected cuttings and different hormonal treatments on the risogenesis induction, on a number of cuttings and on the quality of the root system (length and thickness of the lateral roots and the number of second line roots). The cuttings were treated with three types of plant hormones (IAA, IBA, and NAA) in the mould form, in addition to the control group, without hormonal treatment. The cuttings were raised in containers, in a mixture of the substrate of sphagnum and sand, in 3:1 ratio. From the denominated results, the best hormonal treatment for risogenesis induction of black currant was with IAA for the affected cuttings, which coincides with the results reported by other researches.