In this study we have analyzed the cyclosporine levels in patients after kidney transplantation. The cyclosporine levels are determined by using fluorescence polarization immune-assay and monoclonal whole blood test. We analyzed nine patients with transplanted kidneys who were on specific immunosuppressive cyclosporine therapy. The cyclosporine determination was performed in these patients three times in the span of 14 to 29 days. The cyclosporine levels in seven patients were in reference interval between 100 and 300 ng/ml of whole blood. In the first determination the values in two patients were out of reference interval, one value was lower, and the other one was higher then reference interval. In second determination after dose correction in patients who had lower cyclosporin level the new level was normal and the other patient cyclosporine value was lower then in the first determination, but still a little higher then reference values.

Renal amyloidosis is a rare disease when compared to other kidney diseases. During the period of last fifteen years, at the Institute of Nephrology and Immunology in Sarajevo renal amyloidosis was diagnosed with 15 patients. The disease occurred more often with men than with women. Only during 1988, renal amyloidosis was revealed and followed up with five patients. The most common clinical manifestations of renal amyloidosis are nephrotic syndrome and chronic renal failure, with respective laboratory findings. Using immunofluorescent analysis of the kidney biopsy material, we discovered deposits of immunoglobulins of different intensity and deposits of lambda and kappa light chains of immunoglobulins. The intensity of lambda light chains is greater than that of kappa chains. The analysis of light microscopy showed nodular mesangial deposits and deposits along GBM without proliferation. The diagnosis of amyloidosis was confirmed by staining of amyloid. Application of therapy for amyloidosis was without any effect. Although renal amyloidosis is a rare disease, we want to point out disease as being an etiologic factor in nephrotic syndrome.

The intention of this work is to show the usefulness of use of enzymatic essay in the anti-body detection against GBM in the serum of the Patient with linear deposits of IgG. Full success was obtained. Maximal sensibility of test sensibility and reproduction, as well as the advantage that tests are simply enough for the empiric application in everyday clinical practice was achieved through multiplied vibration of anti-bodies. Test were done 30 patients in the Immunology Laboratory of the Institute for Nephrology in Sarajevo.