Q-Fever nowadays presents the most diffuse disease in the world, caused by the microorganisms from the family Risckettiacae. This disease is Coxiellae burnetii. The laboratory diagnosis of the Q-Fever can be stated either by the isolation of the causer from the patient material, either by the proving of the specifically antibodies. The serologic diagnostics presents the choile method in Q-Fever. The aim of this work is to illustrate the results of the detection of the serum in patients with the clinical symptoms at the infeçtion Coxiellom burnetii. We tested the sera of the patient from the region of the Federation Bosnia and Herzegovina, which arrived in our laboratory in the period of November 2000 till May 2001. From the total 174 prepared sera specific IgM of the antibodies we found in cases, and the specific IgG of the antibodies in 54 sera.

Q-Fever is a worldwide zoonosis caused by Coxiella burnetti. C. burnetti is an obligate intracellular parasite. It lives in phagolysosome of the host cell. By its infection of the sensitive persons develops the acute noncharacteristic disease, which passes noncharacteristically, with the appearance of higher temperature, headache, fever, weakness of the organism or by the appearance of symptoms of the undifferentiated infection of the upper parties of the respiratory system. In the course of the infection is being developed the intersticial pneumonia, what is the reason of the infected hospitalization. Most often get sick the sheep, cows and goats, what showed also on our examined sample. In most animals the symptoms of this bacterial infection are not present, pass unobviously, and get turned out during their gravidity. The most important carriers of the causes of this disease on the domestic or wild animals are artropodes, in which within the kind is possible also the transvatial and transstadial transfer. The wild animals transfer the disease at the domestic ones, and people most often are infected by contact with these animals, their consuming of meat or milk or by contact with their secretions. Though, the most important way of getting infected of people is aerosol contaminated by the carrier as these bacteria for a long get kept in the contaminated dust, wool, animal skin, fur, straw and the excretions of the infected animals. In the illusorilly healthy and pregnant animals the bacteria are to be found in the fertile water, chorions, and placenta, that is C. burnetti becomes the cause of the premature birth or abortion in these animals. In this way comes to the bacterial contamination of the environment of the animal itself. The diagnosis of Q.-Fever is complement fixation test, indirect immunofluorescence assay (IFT) and enzyme immunoassay (EIA).

Human Cytomegalovirus are a ubiquitous herpesvirus establishing virus-infections which are usually asymptomatic in immunocompetent individuals. In patients with Acquired Immunodeficiency Syndrome (AIDS) and immunocompromised hosts, the virus causes primary, latent or chronic persistent infection. Primary CMV infection is very severe in immunocompromised patients as well as among healthy population. Among patients with AIDS Cytomegalovirus (CMV) is usually isolated from patients specimen in association with other pathogens (Pneumocystis carinii, Candida albicans). The prevalence of serious CMV (chorioretinitis, gastrointestinal disease, interstitial pneumonia and central nervous system disease), in AIDS population are respectable.

OBJECTIVES After four years of Sarajevo siege, the deblocade started on July 1995. Many soldiers involved in the deblocade developed a clinical symptoms of hemorrhagic fever indicating a possible epidemic. METHODS Suspected patients were treated in the war hospital Igman-Fojnica. Blood samples of all the patients were processed on IgM and IgG antibodies with ELISA test, using "the double sandwich" technique. RESULTS IgM and IgG were performed on Puumala (PVV), Hantaan (HTN) and Dobrava antigens. 38 out of 45 treated serums had high antibody titres. Sera of 28 patients had high titres of specific IgM antibodies on Hantaan antigen (12,800). A ten patients had a same titre level for specific antibodies of Puumala antigen. A 20 patients had specific IgG antibodies on Dobrava antigen with the titre 400. Our results confirmed the epidemic for which were responsible two serotypes of HFRS-PVV and HTN. They also proved the existence of a new serotypes appearing for the first time in Sarajevo region. This epidemic confirms that BiH especially Sarajevo region are among the biggest epidemic areas of HFRS in Europa.

In this report we proved that all of three methods for laboratory diagnosis of HSV infections which we used, have approximately the same degree of sensibility. Even though there is no statistically significant differences among sensibility of chosen methods, we think that IF test by monoclonal antibodies is the most suitable method for the routine work, in consideration of the high sensibility, typical specificity and fast results.