Metabolic derangements in Type 2 diabetes mellitus (T2D) are associated primarily with the carbohydrate and lipid levels disturbances. Increased flow of free fatty acids (FFAs) into the blood that is coming from the adipocytes as well as an elevated flux of FFAs from de novo lipid synthesis in the liver contribute to these metabolic disturbances. Previous studies suggested a strong association of the hepatic activity of certain enzymes, such as aspartate and alanine transferase (AST, ALT), gamma glutamyl transpeptidase (GGT), and alkaline phosphatase (ALP) with the progression of T2D. In this study, the potential association of the hepatic activities of the liver enzymes and FFAs levels in T2D was examined. Analysis of the activities of ALT, AST, GGT and AP, as well levels of FFAs, fasting plasma glucose (FPG), and lipid profile was performed in 40 healthy control and 71 diabetic subjects. All participants were free of hepatitis, viral infections or active liver damage. The results showed a positive association between levels of palmitic and oleic acids with ALT activity (p<0.05), while the activity of GGT was significantly associated with the levels of palmitic, stearic, and oleic fatty acids (p<0.01). Interestingly, in control group, a positive correlation was found between palmitoleic acid levels with ALT activity (p<0.05), and a negative correlation palmitoleic acid with ALP activity (p<0.05) was observed. Obtained data suggest that an elevation of free fatty acid levels and the hepatic fat accumulation in insulin-resistant conditions affect the hepatic enzymes activities, which might contribute further to the progression of Type 2 diabetes and its complications.

Magnesium is an essential element and the intracellular divalent cation involved in many biochemical functions. People with magnesium deficiency must increase their intake of magnesium, usually in the form of various supplements. A common form of magnesium supplement widely available in pharmacies is magnesium oxide (MgO). In this work, the content of MgO was determined in pharmaceutical supplementations using spectrophotometry, based on the reaction between magnesium ions and eriochrome black T at a wavelength of 535 nm. The analysed content of MgO ranged from 360.5 to 386.5 mg MgO, which corresponds to the daily Mg recommended values (300 to 400 mg).

Introduction: Several decades of basic science and animal research provided considerable support for significant role of plasma free fatty acids (FFAs) in etiology of Type 2 diabetes mellitus (T2DM). Contradicting data related to signifi cance of elevated FFAs in plasma of patients with Type 2 diabetes prompted us to study concentrations of palmitic acid, stearic acid, and linoleic acid, in patients and healthy controls in an attempt to possibly use them as potential biomarkers in progression of the disease. Since aging is associated withincreased plasma glucose and insulin levels that are consistent with an insulin resistant state, in this study,age differences in the concentration of the above mentioned acids were tested.Methods: Progressive changes in their concentrations were followed through a period 6 months. All subjects included in the study were free of evidence of hepatitis B or C viral infection or active liver and kidney damage. Analysis of glucose and glycated hemoglobin levels were performed on BT PLUS 2000 analyzer using standard IFCC protocols, while concentrations of FFAs were analyzed by gas chromatography.Results: Our data demonstrated signifi cantly higher FFA values in plasma of diabetic patients as compared to healthy controls. There was a trend of correlation of FFAs levels with the blood glucose levels in diabetic patients, which was more prominent in diabetic men than in women.Conclusion: With aging, levels of free fatty acids signifi cantly increased in plasma of diabetic patients, and this effect was also more profound in male than in female diabetics.