Introduction: The purpose of the current study was to estimate the biocompatibility of endodontic sealers with different bases on L929 mouse fibroblasts permanent cell line using Multiscan EX Spectrophotometer. Materials and Methods: Endodontics sealers used in this study were GuttaFlow (Roeko) silicone based sealer, AH plus (De Tray-DENTSPLY) epoxy resin based, Apexit (Vivadent) calcium hydroxide based and Endorez (Ultradent) methacrylate based sealer. Sealer were tested trough time, freshly mixed 24 h, 48h and 7 days after setting. Biocompatibility was determinate on permanent cell lines L929 mouse fibroblasts trough cytotoxicity using MTT assay. Level of absorption was measured with multi scan EX spectrophotometer on length 420-600 nm. Results: Sealer based on calcium hydroxide Apexit Plus, GuttaFlow silicone based sealer and AH plus epoxy resin based sealer, have shown a low cytotoxicity through the all periods of time on culture of L292 mouse fibroblasts. Methacrylate based sealer, Endorez showed moderate cytotoxicity when freshly mixed and after 7 days. After 24 hours the visibility of the cells was 74,0% and after 48 hours 65,1%. which is slightly cytotoxic. Conclusions: According to results of this study there is a statistically significant difference among the groups p<0,05 for all the tested sealers. Apexit Plus, GuttaFlow and AH plus can be considered as biocompatibile. EndoREZ sealer which is based on methacrylate, after 7 days shows 50,1% of visible live cells which is considered as moderate cytotoxicity.

The goal of this in vitro study was to evaluate the relative biocompatibility of four endodontic sealers on the cell culture of the human fibroblast through cytotoxicity. Materials and Methods: In this study four endodontics sealers was used GuttaFlow (Roeko)silicone based sealer, AH plus (De Tray-DENTSPLY) epoxy resin based, Apexit (Vivadent) calcium hydroxide based and Endorez (Ultradent) methacrylate based sealer. Sealers were tested on primary cell lines of human gingival fibroblasts. Experiments were preformed in laboratories of Hacettepe University of Ankara, Turkey and Faculty of Dentistry, University of Sarajevo, Bosnia and Herzegovina Cytotoxicity was determinate using WST-1 assay. Results: Results were analyzed by SPSS 19 program. Kolgomorov-Smirnov test, Shapiro-Wilk and descriptive statistics also were used, as well as Kriskall-Wallis, ANOVA test and T- test. According to our results all four sealers showed different cytotoxicity effects on human gingival fibroblast cell culture, but all of them are slightly cytotoxic. Conclusions: According to results of this study it can be concluded: all four sealers showed different cytotoxicity effects on primary cell lines of human gingival fibroblasts, but all of them are slightly cytotoxicity.

Resumen pt: Objeti vo: Determinar a incidencia de carie de radiacao em pacientes com câncer de cabeca e pescoco na populacao Bosnia. Metodo: Trinta pacientes com dia...

Radiotherapy has an important role in the treatment of head and neck carcinoma, but it may result in severe dento-oral adverse effects of the radiation. The purpose of this research was to determine the incidence of radiation induced mucositis in conventionally treated patients with head and neck cancer in Bosnian population. A number of 30 patients with malignant diseases were included into this study, from the Maxillofacial Surgery Clinic and Otorhinolaryngology Clinic. All the patients were treated with radiotherapy in the region of head and neck, at the Institute for Oncology, Clinical Centre of University in Sarajevo. Ass well, these thirty patients were examined in four time periods: before the radiation, 3 weeks after commencing the radiation, three months after commencing the radiation and six months after commencing the radiation. After 3 weeks commencing the radiation, 21 (70%) patients developed radiation mucositis with ulcerations and pseudomembranes. Three months after commencing the radiation 7 (23%) patients had mucositis characterized by erythema and six months after commencing the radiation therapy only one (3%) patient had mucositis. Radiation mucositis is only one of many dento-oral complications affecting patients undergoing radiation therapy for head and neck cancer. All complications have a tremendous impact on their quality of life during and after radiotherapy. Therefore, it would be necessary to improve dental and oral following up and treatment of the patients during the radiation therapy, which has not been case in Bosnia and Herzegovina.